Article
Biophysics
Ee-Seul Kang, Huijung Kim, Yoojoong Han, Yeon-Woo Cho, Hyungbin Son, Zhengtang Luo, Tae-Hyung Kim
Summary: Graphene derivatives show promise for stem-cell-based regenerative therapies, especially in bone regeneration. A graphene oxide-based hybrid platform was developed to guide the osteogenesis of human adipose-derived mesenchymal stem cells effectively. The GOHP exhibited efficiency comparable to high-quality CVD-grown graphene in inducing osteogenesis by upregulating specific genes.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Review
Biochemistry & Molecular Biology
Laidi Wu, Zhixin Liu, Li Xiao, Mi Ai, Yingguang Cao, Jing Mao, Ke Song
Summary: This review comprehensively elucidates the specific roles of Gli1+ MSCs in craniofacial bone osteogenesis and tissue repair, offering new insights into bone regeneration therapy.
Review
Cell Biology
Huan Zhou, Lei Zhang, Yue Chen, Chun-Hui Zhu, Fa-Ming Chen, Ang Li
Summary: Bone formation is a complex regeneration process regulated by multiple signaling pathways, with the Hedgehog (Hh) pathway playing a crucial role. Dysregulation of the Hh pathway in adults is associated with bone-related diseases, while during repair processes, it can be reactivated to modulate bone formation. Current therapeutic strategies targeting the Hh cascade for osteogenesis coordination have been reviewed.
CELL PROLIFERATION
(2022)
Article
Biochemistry & Molecular Biology
Brandon Davis, Peng Shi, Erin Gaddes, Jinping Lai, Yong Wang
Summary: This study investigated the reversible display of supramolecular nanomaterials on the surface of living cells. The data showed that DNA initiators could induce the self-assembly of DNA-alginate conjugates to form supramolecular nanomaterials and amplify the fluorescence signals on the cell surface. Complementary DNA (cDNA), DNase, and alginase could all trigger the reversal of the signals from the cell surface.
Article
Biochemistry & Molecular Biology
Daniel Garcia-Sanchez, Alberto Gonzalez-Gonzalez, Itziar Alvarez-Iglesias, Monica del Dujo-Gutierrez, Alfonso Bolado-Carrancio, Matilde Certo, Maria Isabel Perez-Nunez, Jose A. Riancho, Jose Carlos Rodriguez-Rey, Jesus Delgado-Calle, Flor Maria Perez-Campo
Summary: The secretome from MSCs in which Sfrp1 has been silenced shows positive effects on bone regeneration by enhancing osteogenic differentiation, decreasing the expression of Wnt inhibitor Dkk1, and inhibiting osteoclastogenesis. The secretome also increases mineralization in human primary MSCs from osteoporotic patients. Proteomic analysis reveals enrichment of osteogenesis-related proteins in the secretome, suggesting that this approach can enhance the osteo-regenerative properties of MSCs' secretome.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Kangkang Zha, Yue Tian, Adriana C. Panayi, Bobin Mi, Guohui Liu
Summary: Bone tissue engineering offers a new approach to treating bone defects, with mesenchymal stem cells being a promising seed cell due to their functions in osteogenic differentiation. Improving methods of origin selection, culture conditions, and biophysical stimulation can enhance MSC osteogenesis and contribute to the development of MSC-based bone tissue engineering.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Haoyu Zhao, Yachao Jia, Feng Wang, Yimin Chai, Chunfu Zhang, Jia Xu, Qinglin Kang
Summary: In this study, mesoporous silica-coated magnetic nanoparticles doped with cobalt ions were synthesized to accelerate bone repair. The results showed that local injection of these nanoparticles significantly accelerated bone healing in a mouse model. This study’s findings are important for expediting the treatment process of bone fractures.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Veterinary Sciences
Antonella Liza Pantaleoni Andrietti, Sushmitha S. Durgam, Brittany Naumann, Matthew Stewart
Summary: This study compared osteogenesis in equine bone marrow-, synovium- and adipose-derived cells and found that bone marrow-derived cells had higher basal expression of Osterix, indicating their higher osteogenic potential. In osteogenic medium, these cells quickly developed dense multicellular aggregates with strong mineralization and alkaline phosphatase activity. On the other hand, synovial and adipose cell cultures showed less matrix mineralization.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Review
Cell Biology
Jing Wang, Yi Zhang, Jin Cao, Yi Wang, Nadia Anwar, Zihan Zhang, Dingmei Zhang, Yaping Ma, Yin Xiao, Lan Xiao, Xin Wang
Summary: The skeletal system is crucial for muscle attachment, organ protection, mineral storage, hematopoiesis, and participation in the endocrine and immune system. Autophagy plays a vital role in bone regeneration by controlling molecular degradation and organelle renewal. Autophagy is involved in various bone diseases and can be modulated through autophagy regulators and the use of biomaterials. Understanding autophagy in bone diseases has significant clinical implications.
Review
Engineering, Biomedical
Ziyi Feng, Meiqi Jin, Junzhi Liang, Junning Kang, Huazhe Yang, Shu Guo, Xiaoting Sun
Summary: Bone damage may require bone grafting and bone tissue engineering can be an alternative strategy. Mesenchymal stem cells (MSCs) are important for tissue engineering due to their ability to differentiate into various cell types. The regulation of MSCs differentiation and the role of mitochondria in this process are crucial for efficient bone regeneration.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Physical
Xiaobin Guo, Jiaxiang Bai, Gaoran Ge, Zhidong Wang, Qing Wang, Kai Zheng, Huaqiang Tao, Lirong Zhang, Hongbo Zhang, Dongqing Wang, Xiaogang Zhang, Hongxia Li, Guoqing Pan, Dechun Geng
Summary: The study showed that peptide-decorated titanium implants inspired by mussel adhesion design can effectively inhibit inflammation around implants, promote bone formation, prevent prosthesis loosening, and improve clinical outcomes.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Endocrinology & Metabolism
Ziyi Li, Yafei Wang, Shilun Li, Yukun Li
Summary: Bone regeneration is a complex process involving the coordination of osteogenesis and osteoclastogenesis, with the balance between osteogenesis and adipogenesis of BMSCs playing a critical role. Recent studies have shown that exosomes from M2 macrophages facilitate osteogenesis and reduce adipogenesis through the miR-690/IRS-1/TAZ axis, potentially serving as a therapeutic tool for bone loss diseases.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lukas Skoloudik, Viktor Chrobok, Jan Laco, Jana Dedkova, Daniel Diaz Garcia, Stanislav Filip
Summary: This study evaluated the effect of cyclosporin A (CsA) and a combined biomaterial in the healing process of postoperative temporal bone defects in an animal model. The results showed that the therapeutic efficacy of the combination of MSCs with CsA is greater than the sole application of MSCs in a hydroxyapatite carrier.
Article
Anatomy & Morphology
Yawen Cheng, Yangge Du, Xiao Zhang, Ping Zhang, Yunsong Liu
Summary: Craniofacial bone defects cause significant problems to patients, but bone regeneration using mesenchymal stem cells (MSCs) can be a potential solution. However, the properties of craniofacial bone tissue MSCs are different from MSCs derived from long bones, and the role of CDC20 in craniofacial bone tissue is unknown. This study found that conditional knockout of Cdc20 in mice resulted in distinctive cranial and mandibular bone loss, and impaired osteogenic differentiation potential of cranial suture-derived MSCs and mandibular bone marrow-derived MSCs. These findings provide new insights into craniofacial bone regeneration and the treatment of craniofacial bone-related diseases.
Article
Chemistry, Multidisciplinary
Phillip Chambers, Monika Ziminska, Ahmed Elkashif, Jordan Wilson, John Redmond, Antzela Tzagiollari, Cole Ferreira, Auden Balouch, Jasmine Bogle, Seth W. Donahue, Nicholas J. Dunne, Helen O. Mccarthy
Summary: Bone-related injuries and diseases are a common cause of morbidity worldwide. This study demonstrates the potential of using microRNA (miRNA) gene therapy, specifically miR-26a, for bone healing. The researchers found that delivering miR-26a using the peptide RALA was effective in regulating osteogenic signaling in mesenchymal stem cells. They also showed that treating critical bone defects with RALA/miR-26a nanoparticles resulted in increased bone volume, density, and blood vessel formation. These findings highlight the utility of miRNA gene therapy in promoting bone healing, calling for further investigation in in vivo models.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Engineering, Biomedical
Rachit Agarwal, Tiago M. Volkmer, Peiyi Wang, L. Andrew Lee, Qian Wang, Andres J. Garcia
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2016)
Article
Chemistry, Multidisciplinary
Yingjuan Sun, Guihua Xiao, Yuan Lin, Zhaohui Su, Qian Wang
Article
Chemistry, Analytical
Kaylee R. Mastrianni, L. Andrew Lee, William E. Brewer, Nagaraju Dongari, Michael Barna, Stephen L. Morgan
JOURNAL OF ANALYTICAL TOXICOLOGY
(2016)
Article
Chemistry, Analytical
Pongkwan Sitasuwan, Cathleen Melendez, Margarita Marinova, Kaylee R. Mastrianni, Alicia Darragh, Emily Ryan, L. Andrew Lee
JOURNAL OF ANALYTICAL TOXICOLOGY
(2016)
Article
Polymer Science
Xiaolei Zhang, Xia Zhao, Jittima Amie Luckanagul, Jing Yan, Yuzhe Nie, L. Andrew Lee, Qian Wang
Article
Biotechnology & Applied Microbiology
Xiaolei Zhang, Pongkwan Sitasuwan, Gary Horvath, Jia Yang, Yuzhe Nie, Margarita Marinova, L. Andrew Lee, Qian Wang
ENZYME AND MICROBIAL TECHNOLOGY
(2018)
Article
Chemistry, Analytical
Pongkwan Sitasuwan, Cathleen Melendez, Margarita Marinova, Michelle Spruill, L. Andrew Lee
JOURNAL OF ANALYTICAL TOXICOLOGY
(2019)
Article
Biochemical Research Methods
B. Todd Mullis, Sunil Hwang, L. Andrew Lee, Anton Iliuk, Rebekah Woolsey, David Quilici, Qian Wang
Article
Biochemical Research Methods
Patrick A. Kates, John J. Tomashek, David A. Miles, L. Andrew Lee
Article
Biotechnology & Applied Microbiology
Caleb R. Schlachter, Amanda C. McGee, Pongkwan N. Sitasuwan, Gary C. Horvath, Nanda G. Karri, L. Andrew Lee, John J. Tomashek
Summary: Researchers improved the activity of β-GUS on drug metabolites by designing enzyme chimeras and site-saturation variants, and discovered a chimera from enzymes of different species that exhibited a significant increase in activity on morphine-3-glucuronide.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Article
Chemistry, Analytical
L. Andrew Lee, Amanda C. McGee, Pongkwan Sitasuwan, John J. Tomashek, Chris Riley, Ana Celia Munoz-Munoz, Lawrence Andrade
Summary: Different enzymes exhibit varying performances in clinical urine specimens, showing distinct substrate preferences and pH optima. Urine-specific properties affect the hydrolytic effectiveness of beta-glucuronidases, with some enzymes being more significantly impacted. Diluting urine with buffer greatly improves achieving target pH and reduces the impact of endogenous compounds on enzyme performance.
JOURNAL OF ANALYTICAL TOXICOLOGY
(2022)
Article
Biochemical Research Methods
Yuko Ogata, Pamela M. Quizon, Nancy S. Nightlinger, Pongkwan Sitasuwan, Casey Snodgrass, L. Andrew Lee, Jeffrey D. Meyer, Richard S. Rogers
Summary: The study presented a fully automated MAM sample preparation protocol, achieving rapid desalting using miniaturized size-exclusion chromatography columns on an automated liquid handler. Compared to manual preparation, this automated method showed no significant change in product attributes and their quantities, while enhancing sample recovery and reproducibility.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2022)
Article
Biochemistry & Molecular Biology
Kangjing Wang, Liting Zhao, Ting Li, Qian Wang, Zhongyang Ding, Weifu Dong
Summary: The application of Ni-chelated ion exchange resin in enzyme immobilization and protein purification is reported in this paper. Acrylic weak acid cation exchange resin (D113H) was selected as the carrier resin, and phosphomannose isomerase (PMI) was successfully immobilized on Ni-chelated D113H resin. The immobilized enzyme showed excellent reusability and retained 92% of its initial activity. Additionally, PMI was successfully purified using an affinity chromatography column prepared by Ni-chelated D113H resin, indicating the potential for combining immobilization and purification in one step.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Caleb R. Schlachter, Andrea O'Malley, Linda L. Grimes, John J. Tomashek, Maksymilian Chruszcz, L. Andrew Lee
Summary: Sulfatases are enzymes that hydrolyze sulfate from sulfated organic substrates. They can be used in biotechnology to analyze sulfated metabolites in humans. This study focused on the characterization of a sulfatase from Pedobacter yulinensis and found new activities that are advantageous for biotechnological applications.
Article
Medicine, Research & Experimental
Pongkwan Sitasuwan, Thomas W. Powers, Tiffany Medwid, Yuting Huang, Bradley Bare, L. Andrew Lee
Summary: The multi-attribute method (MAM) is a recent advancement in liquid chromatography-mass spectrometry for simultaneous monitoring of multiple product quality attributes in the pharmaceutical industry. While MAM offers automated data processing and reporting, its sample preparation remains cumbersome and low throughput.