Data Descriptor: Generation and characterization of thiol-deficient Mycobacterium tuberculosis mutants

Mycothiol (MSH) and ergothioneine (ERG) are thiols able to compensate for each other to protect mycobacteria against oxidative stress. Gamma-glutamylcysteine (GGC), another thiol and an intermediate in ERG biosynthesis has detoxification abilities. Five enzymes are involved in ERG biosynthesis, namely EgtA, EgtB, EgtC, EgtD and EgtE. The role of these enzymes in the production of ERG had been unclear. On the other hand, the enzyme MshA is known to be essential for MSH biosynthesis. In this manuscript, we describe the raw data of the generation and characterization of Mycobacterium tuberculosis (M.tb) mutants harbouring a deletion of the gene coding for each of these enzymes, and the raw data of the phenotypic characterization of the obtained thiol-deficient M.tb mutants. High throughput screening (HTS) of off-patent drugs and natural compounds revealed few compounds that displayed a higher activity against the thiol-deficient mutants relative to the wild-type strain. The mode of action of these drugs was further investigated. Raw data displaying these results are described here. Design Type(s) parallel group design . genetic modification design . replicate design Measurement Type(s) metabolite profiling . Impaired Cell Membrane Integrity . regulation of membrane lipid distribution . population growth phenotype . oxidative stress assay . enzyme activity assay . cellular response to nitrosative stress . aggregation of unicellular organisms . biofilm formation Technology Type(s) liquid chromatography- tandem mass spectrometry . flow cytometry assay . scanning electron microscopy . spectrophotometer . microplate reader . multimode microplate reader . photography Factor Type(s) Species . genotype . Organism Strain . replicate . experimental condition Sample Characteristic(s) Mycobacterium tuberculosis . Mycobacterium bovis . Mycobacterium smegmatis