期刊
SPRINGERPLUS
卷 2, 期 -, 页码 -出版社
SPRINGER INTERNATIONAL PUBLISHING AG
DOI: 10.1186/2193-1801-2-583
关键词
Ethanol production; Ethanol tolerance; Molasses; Mutagenesis; Saccharomyces cerevisiae
资金
- Kasetsart University Research and Development Institute (KURDI)
- Kasetsart University, Thailand
- Research Promotion and Technology Transfer Center (RPTTC), Faculty of Liberal Arts and Science, Kasetsart University Kamphaeng Saen Campus, Thailand
Ethanol tolerance is one of the important characteristics of ethanol-producing yeast. This study focused on the improvement of ethanol tolerance of Saccharomyces cerevisiae NR1 for enhancing ethanol production by random UV-C mutagenesis. One ethanol-tolerant mutant, UVNR56, displayed a significantly improved ethanol tolerance in the presence of 15% (v/v) ethanol and showed a considerably higher viability during ethanol fermentation from sugarcane molasses and sugarcane molasses with initial ethanol supplementation. A maximum ethanol concentration produced from molasses medium at 37 degrees C by UVNR56 was 10.3% (v/v), productivity of 1.7 g/l/h and a theoretical yield of 98.7%, while the corresponding values for the wild-type were 8.6% (v/v), 1.4 g/l/h and 83.3%, respectively. In addition, during molasses fermentation under initial supplementation of 5% (v/v) ethanol, the maximum ethanol concentration and productivity of UVNR56 was 25.7% and 42.9% higher than the wild-type, respectively.
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