期刊
IMA FUNGUS
卷 6, 期 2, 页码 363-372出版社
BMC
DOI: 10.5598/imafungus.2015.06.02.08
关键词
Ascomycota; DNA isolation; insect collections; Laboulbeniales-specific primers; ribosomal DNA; unculturable fungi
类别
资金
- Harvard University Graduate School of Arts and Sciences
- American Museum of Natural History (Theodore Roosevelt Memorial Grant)
- National Park Service
- 'AntLab' Marie Curie Career Integration Grant within the 7th European Community Framework Programme
- 'Bolyai Janos' scholarship of the Hungarian Academy of Sciences (MTA)
Laboulbeniales is one of the most peculiar orders of Ascomycota. These fungi are characterized by an ectoparasitic life-style on arthropods, determinate growth, lack of an asexual stage, high species richness, and intractability to culture. The order Laboulbeniales, sister to Pyxidiophorales, has only recently been assigned a separate class, the Laboulbeniomycetes, based on very few ribosomal DNA sequences. So far, DNA isolations and PCR amplifications have proven difficult. Here, we provide details of isolation techniques and the application of commercially available kits that enable efficient and reliable genetic analyses of these fungi. We provide 43 newly generated Laboulbeniales ribosomal DNA sequences, among which are the first published sequences for species in the genera Gloeandromyces, Herpomyces, Laboulbenia, Monoicomyces, and Polyandromyces. DNA extractions were possible using from 1 to 30 thalli from hosts preserved in ethanol (70-100 %). In two cases, we successfully isolated DNA from thalli on dried insect collections. Laboulbeniales molecular systematics could be substantially enhanced through these improved methods by allowing more complete sampling of both taxa and gene regions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据