Article
Biochemistry & Molecular Biology
Scarlett Puebla-Barragan, Emiley Watson, Charlotte van der Veer, John A. Chmiel, Charles Carr, Jeremy P. Burton, Mark Sumarah, Remco Kort, Gregor Reid
Summary: The study found that different strains of Lactobacillus crispatus in the vagina can produce or degrade different biogenic amines, leading to varying effects on vaginal pH and pathogens. Additionally, strains isolated from women with dysbiotic vaginal microbiota showed stronger antimicrobial effects against certain pathogens.
Article
Cell Biology
Ping Li, Kehong Wei, Xia He, Lu Zhang, Zhaoxia Liu, Jing Wei, Xiaomei Chen, Hong Wei, Tingtao Chen
Summary: The study showed that L. crispatus and other bacteria tested significantly affected sperm activity and vaginal microbiota composition, resulting in reduced pregnancy rates and impacts on offspring health.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Chenchen Ding, Huixian Wu, Xiuyun Cao, Zhangshan Gao, Zhihui Tang, Wentao Fan, Liping Yan, Bin Liu, Hong Lin, Suquan Song
Summary: A novel heteropolysaccharide (EPS 7-4) was isolated from Lactobacillus crispatus in this study. EPS 7-4 exhibited excellent antibacterial and anti-inflammatory abilities, inhibiting the proliferation, biofilm formation, and virulence factor gene expression of Salmonella typhimurium. It also effectively restricted bacterial translocation, upregulated the abundance of beneficial bacteria, and alleviated severe inflammatory response in the intestinal tract of mice.
Article
Biochemistry & Molecular Biology
Rosanne Hertzberger, Ali May, Gertjan Kramer, Isabelle van Vondelen, Douwe Molenaar, Remco Kort
Summary: Glycogen in the female lower reproductive tract is an important carbon source for colonization and acidification by vaginal Lactobacillus crispatus. This study characterizes the genetic variation and regulation of the pulA gene, which correlates with the ability to utilize glycogen for growth. The findings suggest that environmental and genetic factors contribute to the variation of Lactobacillus crispatus alpha-glucosidases in the vaginal environment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Anthony J. Kyser, Mohammadali Masigol, Mohamed Y. Mahmoud, Mark Ryan, Warren G. Lewis, Amanda L. Lewis, Hermann B. Frieboes, Jill M. Steinbach-Rankins
Summary: Bacterial vaginosis (BV) is characterized by low levels of lactobacilli and overgrowth of potential pathogens in the female genital tract. Three-dimensional (3D)-bioprinting of Lactobacillus crispatus-containing gelatin alginate scaffolds provides sustained release and proliferation of live bacteria over 28 days, without impacting viability of vaginal epithelial cells. This study presents a novel strategy using 3D-bioprinted scaffolds for long-term probiotic delivery and restoration of vaginal lactobacilli.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Infectious Diseases
Carola Parolin, Angela Abruzzo, Barbara Giordani, Josidel C. Oliver, Antonella Marangoni, Barbara Luppi, Beatrice Vitali
Summary: The study investigated the anti-Candida potential of HA and LCFS from Lactobacillus crispatus BC5, showing their effectiveness against clinical Candida isolates and even species generally resistant to conventional antifungals. The development of a vaginal formulation containing both agents demonstrated promising mucoadhesive ability and almost complete inhibition of Candida albicans growth, suggesting a new therapeutic strategy for VVC.
Article
Biochemistry & Molecular Biology
Farzaneh Fakharian, Amir Sadeghi, Farkhondeh Pouresmaeili, Neda Soleimani, Abbas Yadegar
Summary: This study aimed to investigate the inhibitory and modulatory effects of live and pasteurized Lactobacillus crispatus strain RIGLD-1 on H. pylori adhesion, invasion, and inflammatory response in AGS cell line. The results showed that both live and pasteurized L. crispatus significantly decreased H. pylori adhesion/invasion to AGS cells and modulated H. pylori-induced inflammation. Therefore, live and pasteurized L. crispatus may be potential probiotic candidates against H. pylori colonization and inflammation.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Jie Zhang, Lili Li, Tong Zhang, Jin Zhong
Summary: In this study, a new type of enzyme called GlgU was identified in L. crispatus, which showed optimal activity at acidic pH and exhibited substrate specificity towards glycogen. This finding contributes to the understanding of glycogen metabolism in the dominant species of human vaginal microbiota.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Maximilien Clabaut, Amandine Suet, Pierre-Jean Racine, Ali Tahrioui, Julien Verdon, Magalie Barreau, Olivier Maillot, Agathe Le Tirant, Madina Karsybayeva, Coralie Kremser, Gerard Redziniak, Cecile Duclairoir-Poc, Chantal Pichon, Sylvie Chevalier, Marc G. J. Feuilloley
Summary: Research shows that 17 beta-estradiol has direct effects on Lactobacillus crispatus, affecting membrane dynamics and promoting biosurfactant production and adhesion to vaginal cells. These effects are important for the physiology of the vaginal environment, contributing to lactobacillus adhesion to mucosa and protection against pathogens.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Takeshi Kawahara, Issei Shimizu, Yuuki Tanaka, Keisuke Tobita, Mikado Tomokiyo, Itsuki Watanabe
Summary: This study found that SLPs from Lactobacillus crispatus KT-11 can inhibit human rotavirus infection of intestinal epithelial cells. KT-11 LE showed a dose-dependent suppressive effect on DS-1 infection with pre-infection treatment, while KT-11 SLP concentrate significantly inhibited the proliferative process of DS-1 strain. These results provide crucial evidence for the antiviral effect of SLPs.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Chiara Argentini, Federico Fontana, Giulia Alessandri, Gabriele Andrea Lugli, Leonardo Mancabelli, Maria Cristina Ossiprandi, Douwe van Sinderen, Marco Ventura, Christian Milani, Francesca Turroni
Summary: This study investigates the colonization mechanism and antimicrobial activity of Lactobacillus crispatus in the vaginal ecological niche. The results reveal the genetic variability of this species and its adaptation to the niche to enhance competitiveness for colonization.
MICROBIOLOGY SPECTRUM
(2022)
Article
Food Science & Technology
Margherita D'Alessandro, Flavia Pisanu, David Baldo, Carola Parolin, Gianfranco Filippini, Beatrice Vitali, Rosalba Lanciotti, Francesca Patrignani
Summary: The study found that all microbial mixtures exhibited high viability for 90 days of storage, and there was a more significant decrease in cell viability for non-encapsulated microbial combinations compared to the encapsulated strains in the in vitro digestion results.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Microbiology
Annelot C. Breedveld, Heleen J. Schuster, Robin van Houdt, Rebecca C. Painter, Reina E. Mebius, Charlotte van der Veer, Sylvia M. Bruisten, Paul H. M. Savelkoul, Marjolein van Egmond
Summary: This study found that the vaginal microbiota dominated by Lactobacillus crispatus is associated with higher levels of bacterial IgA coating compared to other microbiota compositions. Similar to its role in the intestinal tract, IgA may be involved in maintaining the L. crispatus-dominated microbiota in the female genital tract, which may contribute to the health benefits associated with L. crispatus.
Article
Microbiology
Youngkyoung Lee, Hoonhee Seo, Sukyung Kim, M. D. Abdur Rahim, Youjin Yoon, Jehee Jung, Saebim Lee, Chang Beom Ryu, Ho-Yeon Song
Summary: Tuberculosis, caused by Mycobacterium tuberculosis, remains a significant public health issue globally with high mortality rates. The emergence of drug-resistant strains poses a threat to public health, and current antibiotic treatments have limited efficacy and severe side effects. Probiotics, such as Lactobacillus crispatus PMC201, show promise as potential therapeutic agents against tuberculosis, with the ability to reduce bacterial load and maintain a healthy microbial ecosystem. Further research is needed to evaluate the in vivo efficacy and mechanism of action of these probiotics as alternative drug candidates in the treatment of tuberculosis.
JOURNAL OF MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Nkechi Martina Odogwu, Chinedum Amara Onebunne, Jun Chen, Funmilola A. Ayeni, Marina R. S. Walther-Antonio, Oladapo O. Olayemi, Nicholas Chia, Akinyinka O. Omigbodun
Summary: This study characterized the vaginal microbiome of Nigerian women and found that E2 and P1 levels significantly influenced the vaginal microbiome, with an increasing trend of L. crispatus abundance correlating with increasing E2 and P1 concentrations during pregnancy. A shift from Lactobacillus to non-Lactobacillus profile was observed postnatally in most women, indicating a species-specific link between pregnancy steroid hormone concentration and L. crispatus abundance.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Eduard Y. Kostetsky, Vladimir N. Uversky
Summary: This study discusses the formation of the matrix mechanism in protocells, focusing on the involvement of mineral complexes and gas phase radicals. The structures of apatite and carbonate-apatite play a role in determining the formation of DNA and RNA, while organic matter gradually replaces the mineral matrix. The study provides a detailed description of the preparation and launch of the matrix mechanism, as well as discussing several related issues.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Letter
Biochemistry & Molecular Biology
Vladimir N. Uversky, Fatma Elrashdy, Abdullah Aljadawi, Elrashdy M. Redwan
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Microbiology
Andrey V. Karlyshev
Summary: The complete genome sequence of Campylobacter jejuni strain X, which contains two plasmids similar to pVir and pTet identified in strain 81-176, was reported. Analysis of other complete genome sequences in GenBank revealed the presence of similar plasmids in several other strains, suggesting a common origin.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Infectious Diseases
Vyacheslav M. Abramov, Igor Kosarev, Andrey Machulin, Tatiana Priputnevich, Irina O. Chikileva, Evgenia Deryusheva, Tatiana N. Abashina, Almira D. Donetskova, Alexander N. Panin, Vyacheslav G. Melnikov, Nataliya E. Suzina, Ilia N. Nikonov, Marina Selina, Valentin S. Khlebnikov, Vadim K. Sakulin, Raisa N. Vasilenko, Vladimir A. Samoilenko, Vladimir N. Uversky, Andrey Karlyshev
Summary: Limosilactobacillus fermentum strain 3872 (LF3872) is a strain isolated from the breast milk of a healthy woman, which exhibits antibacterial activity and immunoregulatory properties. It shows strong bacteriolytic action against Gram-positive pathogens and co-aggregation with Gram-negative pathogens.
Review
Immunology
Kazuhide Takada, Vyacheslav G. Melnikov, Ryoki Kobayashi, Shihoko Komine-Aizawa, Noriko M. Tsuji, Satoshi Hayakawa
Summary: This review discusses the bidirectional communication between the female reproductive tract (FRT) and remote/versatile organs in the body. The framework of the FRT-organ axes, including various axes such as the vagina-gut axis and uterus-brain axis, is comprehensively discussed. Each axis is not only involved in gynecological diseases, but also diseases occurring outside the FRT. The investigation of the FRT-organ axes provides a multi-centric approach to understand the homeostatic mechanism of women's bodies and may lead to new therapeutic approaches for women's health.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ekaterina L. L. Nemashkalova, Marina P. P. Shevelyova, Andrey V. V. Machulin, Dmitry D. D. Lykoshin, Roman S. S. Esipov, Evgenia I. I. Deryusheva
Summary: Vascular endothelial growth factor-A (VEGF-A) is a glycoprotein involved in regulating angiogenesis. This study investigated the effect of heparin (HE) on the structural and physicochemical properties of recombinant human VEGF(165). HE binding increased the exposure of hydrophobic surfaces in VEGF(165) without altering its secondary structure. The binding also led to oligomerization/aggregation of VEGF(165). Molecular modeling was used to understand the mechanism behind the VEGF(165)/HE system.
Article
Microbiology
E. Y. Abidueva, E. B. Kudryashova, E. V. Ariskina, Sh. -W. Liu, Ch. -H. Sun, A. V. Karlyshev
Summary: A gram-stain-negative, rod-shaped and motile bacterium, Alg18-2.2(T), was isolated from a highly alkaline-saline lake in Buryatia, Russia. The bacterium can tolerate high pH and salt concentrations and belongs to the family Lysobacteraceae. Based on phylogenetic and genomic analysis, a new genus and species Alkalisalibacterium limincola gen. nov., sp. nov. is proposed.
Article
Immunology
Ramiro Ortiz Moyano, Fernanda Raya Tonetti, Kohtaro Fukuyama, Mariano Elean, Mikado Tomokiyo, Yoshihito Suda, Vyacheslav Melnikov, Haruki Kitazawa, Julio Villena
Summary: Cp and BLPs can stimulate alveolar macrophages and enhance the humoral immune response induced by the commercial pneumococcal vaccine. Mice immunized with Cp + PCV or BLPs + PCV showed higher levels of specific antibodies and lower levels of pneumococcal cell counts and lung damage compared to the control group. These findings demonstrate the potential of Cp and BLPs as adjuvants to enhance the immune response against respiratory pathogens.
Article
Microbiology
Tatiana Z. Esikova, Tatiana O. Anokhina, Nataliya E. Suzina, Tatiana V. Shushkova, Yonghong Wu, Inna P. Solyanikova
Summary: In this work, a new strain named Ch2 was isolated from polluted soils and found to have the unique capability of utilizing toxic compounds like CAP and GP as carbon and energy sources. Analysis of Ch2's 16S rRNA gene revealed its belonging to the Pseudomonas putida species. The strain could degrade CAP through a megaplasmid, and intensive GP utilization occurred during active growth with unique changes in the cytoplasm, indicating the potential role of metabolosomes. Additionally, Ch2 showed the ability to produce PHAs, highlighting its potential in bioremediation and the production of bioplastics.
Article
Infectious Diseases
Vyacheslav M. Abramov, Igor V. Kosarev, Andrey V. Machulin, Tatiana V. Priputnevich, Evgenia I. Deryusheva, Ekaterina L. Nemashkalova, Irina O. Chikileva, Tatiana N. Abashina, Alexander N. Panin, Vyacheslav G. Melnikov, Nataliya E. Suzina, Ilia N. Nikonov, Marina V. Selina, Valentin S. Khlebnikov, Vadim K. Sakulin, Vladimir A. Samoilenko, Alexey B. Gordeev, Gennady T. Sukhikh, Vladimir N. Uversky, Andrey V. Karlyshev
Summary: LF3872 strain from a lactating woman produces a thermolabile class III bacteriolysin (BLF3872) that exhibits antimicrobial activity against antibiotic-resistant Staphylococcus aureus strains. The study is important for developing new drugs against antibiotic-resistant S. aureus strains circulating in both human and animal environments.
Article
Microbiology
A. A. Belova, E. N. Kaparullina, N. V. Agafonova, D. S. Grouzdev, D. S. Kopitsyn, A. V. Machulin, N. V. Doronina
Summary: A new facultative methylotroph strain, 6x-1(T), was isolated from the phyllosphere of oak using methanol as a medium. It belongs to gram-negative short ovoid rods and has specific physiological and chemical characteristics. Based on gene sequence and genome analysis, this strain represents a new species of the genus Ancylobacter.
Article
Infectious Diseases
Vyacheslav M. Abramov, Igor V. Kosarev, Andrey V. Machulin, Evgenia I. Deryusheva, Tatiana V. Priputnevich, Alexander N. Panin, Irina O. Chikileva, Tatiana N. Abashina, Ashot M. Manoyan, Anna A. Ahmetzyanova, Olga E. Ivanova, Tigran T. Papazyan, Ilia N. Nikonov, Nataliya E. Suzina, Vyacheslav G. Melnikov, Valentin S. Khlebnikov, Vadim K. Sakulin, Vladimir A. Samoilenko, Alexey B. Gordeev, Gennady T. Sukhikh, Vladimir N. Uversky
Summary: The LS7247 strain of Ligilactobacillus salivarius has shown high probiotic potential against Salmonella Enteritidis and Salmonella Typhimurium, with high tolerance to gastric and intestinal stress and strong anti-Salmonella activity. It produces a high concentration of lactic acid and bacteriocins, which contribute to its antimicrobial properties. The cell-free culture supernatant of LS7247 has been shown to inhibit the adhesion of Salmonella pathogens to human and animal enterocytes, and when combined with the prebiotic Actigen, it demonstrates a significant combined effect in inhibiting adhesion.
Article
Nanoscience & Nanotechnology
V. A. Arlyapov, L. S. Kuznetsova, A. S. Kharkova, D. Provotorova, E. D. Nenarochkina, O. A. Kamanina, A. Machulin, O. N. Ponamoreva, V. A. Alferov, A. N. Reshetilov
Summary: The possibility of using redox-active polymers based on bovine serum albumin and chitosan covalently linked to neutral red, thionine, and ferrocene mediators, and containing carbon nanotubes for glucose-oxidase immobilization is investigated. The systems containing a redox-active polymer based on BSA modified with a ferrocene mediator are found to be the most promising. Biosensors formed using nanocomposite matrices have a high sensitivity and high correlation with the results of determining the glucose content in human blood by the standard method.
NANOBIOTECHNOLOGY REPORTS
(2022)