期刊
ONCOIMMUNOLOGY
卷 2, 期 4, 页码 -出版社
LANDES BIOSCIENCE
DOI: 10.4161/onci.23564
关键词
adoptive T cell therapy; CD4(+) T cells; CD8(+) T cells; chNKG2D; ID8; immunotherapy; NKG2D; ovarian cancer; tumor immunology
资金
- National Institutes of Health [CA130911, T32 AI007363]
- Norris Cotton Cancer Center
Conditioning strategies that deplete host lymphocytes have been shown to enhance clinical responses to some adoptive T-cell therapies. However, host T cells are capable of eliminating tumor cells upon the relief of immunosuppression, indicating that lymphodepletion prior to T-cell transfer may reduce optimal tumor protection elicited by cell treatments that are capable of shaping host immunity. In this study, we show that adoptively transferred T cells bearing a chimeric antigen receptor (CAR) harness endogenous T cells for optimal tumor elimination and the development of a tumor-specific memory T cell response. Mice bearing ID8 ovarian cancer cells were treated with T cells transduced with a NKG2D-based CAR. CAR-expressing T cells increased the number of host CD4(+) and CD8(+) T cells at the tumor site in a CXCR3-dependent manner and increased the number of antigen-specific host CD4(+) T cells in the tumor and draining lymph nodes. In addition, the administration of CAR-expressing T cells increased antigen presentation to CD4(+) T cells, and this increase was dependent on interferon and granulocyte-macrophage colony-stimulating factor produced by the former. Host CD4(+) T cells were sufficient for optimal tumor protection mediated by NKG2D CAR-expressing T cells, but they were not necessary if CD4(+) T cells were adoptively co-transferred. However, host CD4(+) T cells were essential for the development of an antigen-specific memory T-cell response to tumor cells. Moreover, optimal tumor elimination as orchestrated by NKG2D CAR-expressing T cells was dependent on host CD8(+) T cells. These results demonstrate that adoptively transferred T cells recruit and activate endogenous T-cell immunity to enhance the elimination of tumor cells and the development of tumor-specific memory responses.
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