Article
Fisheries
Xue Zhang, Jingfeng Sun, Zhuoran Han, Feng Chen, Aijun Lv, Xiucai Hu, Xueliang Sun, Hongli Qi, Yongjun Guo
Summary: Homeostasis of intestinal microbiota is crucial to host health, and pathogen invasion leads to dynamic changes in gut microbes. Vibrio parahaemolyticus is an important pathogen of Pacific white shrimp, causing high mortality. The TJA114 strain lacks the pir gene but showed strong pathogenicity, altering bacterial composition in shrimp gut. Metabolites involved in various pathways were identified, providing new insights into vibriosis pathogenesis in shrimp.
Article
Immunology
Mediha Yildirim-Aksoy, Rashida Eljack, Eric Peatman, Benjamin H. Beck
Summary: This study evaluated the changes in hematology, immunology, and biochemical values in the hemolymph of shrimp infected with Vibrio parahaemolyticus. The results showed that shrimp's immune response was altered, and the hemolymph parameters could be used to assess the health status of shrimp.
MICROBIAL PATHOGENESIS
(2022)
Article
Fisheries
Miles D. Lange, Jason Abernathy, Anna A. Rawles, Dunhua Zhang, Craig A. Shoemaker, Troy J. Bader, Benjamin H. Beck
Summary: Vibrio parahaemolyticus, commonly found in marine and estuarine environments, is a Gram-negative bacterium that causes acute hep-atopancreatic necrosis disease in the shrimp aquaculture industry. The major virulence factors, PirA and PirB proteins, play a significant role in the development of this disease. In this study, recombinant PirA and PirB proteins were administered to Pacific white shrimp, and the host response was investigated through RNA sequencing and gene expression analysis. The results suggest that these proteins initiate an early host response involving cell survival signaling and innate immune processes.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Muhamad Gustilatov, Widanarni, Julie Ekasari, Gde Sasmita Julyantoro Pande
Summary: This study evaluates the application of a biofloc system in protecting Pacific white shrimp from pathogenic Vibrio parahaemolyticus infection. The results show that the biofloc system can significantly reduce the density and biofilm activity of V. parahaemolyticus, and improve the survival, growth, and immune response of the shrimp. This suggests that biofloc system could be an effective method to control vibriosis in shrimp culture.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Chemistry, Applied
Fengyan Liang, Chengbo Sun, Sidong Li, Tingting Hou, Chengpeng Li
Summary: The chitosan-gentamicin conjugate (CS-GT) has been shown to enhance the immune response and disease resistance of Litopenaeus vannamei against Vibrio parahaemolyticus, possibly by stimulating lysosomal phagocytosis of pathogens.
CARBOHYDRATE POLYMERS
(2021)
Article
Microbiology
Yi-Ting Chang, Hao-Ting Ko, Ping-Lun Wu, Ramya Kumar, Han-Ching Wang, Hsiao-Pei Lu
Summary: This study revealed that shrimp gut microbiota responded differently to pathogenic and non-pathogenic Vibrio parahaemolyticus strains, with pathogenic Vibrio-infected shrimp showing enrichment of Photobacterium and Vibrio genera, while non-pathogenic Vibrio-infected shrimp had Candidatus Bacilliplasma enriched. Functional predictions suggested that changes in taxonomic compositions would affect normal metabolic functions, highlighting the importance of maintaining gut microbiota equilibrium. Several biomarkers associated with specific microbial taxa and functional pathways were identified, aiding in predicting disease outcomes.
MICROBIOLOGY SPECTRUM
(2023)
Article
Fisheries
Qian Zhang, Yang Yu, Zheng Luo, Fuhua Li
Summary: In this study, the correlation between hepatopancreas color and the infection process of V. parahaemolyticus was analyzed to find a convenient method for evaluating shrimp resistance to AHPND. The hepatopancreas color changes can reflect the stages of infection process, and help us evaluate shrimp resistance to Vibrio, accelerating the breeding of disease-resistant shrimp.
Article
Fisheries
Shengwen Niu, Yuening Zhu, Ran Geng, Nuo Chen, Mengting Luo, Hongliang Zuo, Linwei Yang, Shaoping Weng, Jianguo He, Xiaopeng Xu
Summary: In this study, a novel Toll receptor, Toll10, has been identified and studied in relation to the immune response to bacterial infection in the pacific white shrimp. Toll10 is mainly located on the cell membrane and its expression is upregulated during bacterial infection. It directly interacts with bacteria, and its silencing increases the susceptibility of shrimp to Vibrio parahaemolyticus infection and inhibits phagocytosis by hemocytes. Toll10 also activates the NF-KB family member Dorsal and regulates the expression of antimicrobial peptide genes.
Article
Microbiology
Yi-Ting Chang, Hao-Ting Ko, Ping-Lun Wu, Ramya Kumar, Han-Ching Wang, Hsiao-Pei Lu
Summary: This study reveals the different responses of shrimp gut microbiota to pathogenic and non-pathogenic Vp strains, with bacterial genera Photobacterium and Vibrio enriched in pathogenic Vp-infected shrimp, and Candidatus Bacilliplasma enriched in non-pathogenic Vp-infected shrimp. Moreover, changes in taxonomic compositions further affect normal metabolic functions, emphasizing the importance of sustaining an equilibrium in the gut microbiota. Several biomarkers associated with specific microbial taxa and functional pathways were identified, which help predict the incidence of disease outcomes.
MICROBIOLOGY SPECTRUM
(2023)
Article
Fisheries
K. G. Aguilar-Rendon, S. A. Soto-Rodriguez, B. Gomez-Gil, R. Lozano-Olvera, B. Yanez-Rivera
Summary: This study investigated the impact of pathogenic strains causing acute hepatopancreatic necrosis disease (AHPND) on the water microbiome. The results showed that the virulence of the strains and the duration of the experiment could alter the water microbiome, particularly leading to a significant reduction in the reads assigned to Neptuniibacter spp. This depletion suggests a possible dual function of the type VI secretion system 1 (T6SS1) in the pathogenic strains, killing surrounding bacteria and damaging the hepatopancreas.
Article
Multidisciplinary Sciences
Yu-Ju Lin, Jui-Jen Chang, Huai-Ting Huang, Chih-Ping Lee, Yeh-Fang Hu, Mao-Lun Wu, Chih-Yang Huang, Fan-Hua Nan
Summary: This study compared the effects of natural astaxanthin derived from Kluyveromyces marxianus (TB) with chemically synthetic astaxanthin (CP and LP) on the red coloration, immune parameters, and resistance to Vibrio infection in white shrimp. Results showed that shrimps fed with AST-supplemented feeds, especially TB100, TB200, and TE200, exhibited improved redness values and higher immune responses.
SCIENTIFIC REPORTS
(2023)
Article
Fisheries
Tirawat Rairat, Niti Chuchird, Arunothai Keetanon, Paolo Carcano, Marcello Comi, Wolfgang Koppe
Summary: Yeast-derived nucleotides (NT) and RNA have positive effects on the growth performance, survival, immune responses, and disease resistance of Pacific white shrimp.
AQUACULTURE REPORTS
(2022)
Article
Fisheries
Yusuf Jibril Habib, Haifu Wan, Yulong Sun, Jialong Shi, Chengjie Yao, Jiaming Lin, Hui Ge, Yilei Wang, Ziping Zhang
Summary: This study conducted a genome-wide study of Toll-like receptor genes in Pacific white shrimps for the first time, identifying 11 TLR genes. The majority of these genes were found to be ubiquitously expressed in various tissues and significantly upregulated at different time points after infection, suggesting their significant roles in the immune response of Litopenaeus vannamei.
Article
Fisheries
Maria Agustina Pardede, Widanarni Widanarni, Sukenda Sukenda, Munti Yuhana
Summary: This study aimed to evaluate the different effects of dietary microencapsulated synbiotics on the growth, intestinal histology, immune responses, and gut microbiota diversity of white shrimp. The results showed that synbiotics with different effects resulted in higher growth performance, wider gut microbiota diversity, improved intestinal histology, and elevated immune responses compared with controls.
AQUACULTURE INTERNATIONAL
(2023)
Article
Fisheries
Guowei Liao, Qiuping Wu, Binhua Mo, Jiang Zhou, Junyi Li, Jixing Zou, Lanfen Fan
Summary: This study investigated the impact of Vibrio alginolyticus on the intestinal histomorphology and microbiome composition in Litopenaeus vannamei. The results showed that Vibrio alginolyticus infection caused exfoliation of intestinal mucosal epithelial cells and altered the tissue morphology of the intestine. It also affected the abundance and diversity of intestinal microbiome.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Niti Chuchird, Tirawat Rairat, Arunothai Keetanon, Putsucha Phansawat, Chi-Chung Chou, Joy Campbell
Summary: This study found that adding 4.5-6% SDP to the diet significantly improved the body weight, survival rate, and feed conversion ratio of Pacific white shrimp. It also enhanced the immune parameters and resistance to bacterial infection in the shrimp.
Article
Veterinary Sciences
Tirawat Rairat, Yi-Kai Liu, Julia Chu-Nin Hsu, Chia-Yu Hsieh, Niti Chuchird, Chi-Chung Chou
Summary: Prudent antimicrobial use requires understanding of pharmacokinetics, which is influenced by water temperature and salinity. This study found that temperature has a larger effect on pharmacokinetics compared to salinity.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Fisheries
Tirawat Rairat, Shih-Min Chen, Yi-Ping Lu, Julia Chu-Nin Hsu, Yi-Kai Liu, Chi - Chung Chou
Summary: The study investigates the advantage of using temperature-dependent optimal dosages of antimicrobials in aquaculture. The findings suggest that adjusting the dosing regimen based on rearing temperature can improve treatment efficacy and reduce withdrawal time, while avoiding over- or under-dosing.
Article
Fisheries
Tirawat Rairat, Sunisa Kumphaphat, Niti Chuchird, Prapansak Srisapoome, Putsucha Phansawat, Arunothai Keetanon, Yi-Kai Liu, Chi-Chung Chou
Summary: This study investigated the pharmacokinetic behavior of florfenicol in Asian seabass cultured at 25 degrees C. The optimal dosage and withdrawal time were determined. The findings can potentially be applied for the treatment of bacterial diseases in farming Asian seabass.
JOURNAL OF FISH DISEASES
(2023)
Article
Chemistry, Applied
Tirawat Rairat, Ming-Kun Hsieh, Wan-Cih Ho, Yi-Ping Lu, Zhu-Ying Fu, Niti Chuchird, Chi-Chung Chou
Summary: Water temperature has a significant impact on drug behavior in Asian seabass. This study investigated the differences in pharmacokinetics, optimal dosages, tissue depletion, and withdrawal time of florfenicol in Asian seabass reared at different temperatures. The results showed that temperature affected the elimination half-life and optimal dosages of the drug, but had minimal impact on tissue depletion and withdrawal time.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2023)
Article
Fisheries
Niti Chuchird, Tirawat Rairat, Arunothai Keetanon, Daranee Seguin, Rutchanee Chotikachinda, Lukas Manomaitis, Chuchai Kanjanamayoon
Summary: This study aimed to investigate the effects of feed enzyme combination and functional immunostimulant combination in improving the overall health of Pacific white shrimp fed a soybean-based diet. The results suggest that the addition of feed enzymes and functional immunostimulants to the diet containing 30% soybean meal improved the overall performance, immune function, and resistance to Vibrio parahaemolyticus infection of the shrimp.
JOURNAL OF THE WORLD AQUACULTURE SOCIETY
(2023)
Article
Pharmacology & Pharmacy
Tirawat Rairat, Ming-Kun Hsieh, Yi-Ping Lu, Wan-Cih Ho, Ho-Chien Wu, Chi-Chung Chou
Summary: Starvation has an impact on the pharmacokinetics of drugs in land animals, and this study examined the effects of starvation on the pharmacokinetics of florfenicol in Asian seabass. Starvation resulted in changes in the absorption, distribution, and elimination rates of florfenicol, leading to a lower optimal dosage in starved fish.
JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS
(2023)
Article
Fisheries
Tirawat Rairat, Niti Chuchird, Arunothai Keetanon, Paolo Carcano, Marcello Comi, Wolfgang Koppe
Summary: Yeast-derived nucleotides (NT) and RNA have positive effects on the growth performance, survival, immune responses, and disease resistance of Pacific white shrimp.
AQUACULTURE REPORTS
(2022)
Article
Fisheries
Tirawat Rairat, Putsucha Phansawat, Arunothai Keetanon, Parattagorn Kachapol, Sunisa Kumphaphat, Lalitphan Kitsanayanyong, Chi-Chung Chou, Niti Chuchird
Summary: The study investigated the effect of tributyrin on the growth, survival, hypoxia tolerance, and nutrient digestibility of Pacific white shrimp fed different levels of soybean meal. Tributyrin supplementation at 0.1% significantly improved the weight gain and survival rate of shrimp fed 25% soybean meal. It also reduced Vibrio spp. counts and enhanced hypoxia tolerance. Overall, tributyrin was beneficial for the health of shrimp, especially those fed moderate levels of soybean meal (25%).
JOURNAL OF THE WORLD AQUACULTURE SOCIETY
(2023)