Review
Medicine, General & Internal
Yu Ding, Beibei Gao, Jinyu Huang
Summary: Mitochondrial tRNA mutations in cardiomyopathy are not well understood, but they may affect mitochondrial function and contribute to disease susceptibility. This review provides an overview of tRNA biology and discusses molecular mechanisms underlying mitochondrial dysfunction caused by tRNA mutations.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Feilong Meng, Mi Zhou, Yun Xiao, Xiaoting Mao, Jing Zheng, Jiaxi Lin, Tianxiang Lin, Zhenzhen Ye, Xiaohui Cang, Yong Fu, Meng Wang, Min-Xin Guan
Summary: This study revealed the pleiotropic effects of a deafness-associated tRNA mutation on mitochondrial functions, such as affecting tRNA structure and function, impairment of mitochondrial translation, respiratory deficiency, and increasing production of reactive oxygen species.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Gui-Xin Peng, Xue-Ling Mao, Yating Cao, Shi-Ying Yao, Qing-Run Li, Xin Chen, En-Duo Wang, Xiao-Long Zhou
Summary: The study found that mitochondrial aminoacyl-tRNA synthetases (mito aaRSs) partially colocalize with mitochondrial RNA granules (MRGs), possibly facilitated by their tRNA-binding capacity. Additionally, several mito aaRS-containing complexes were discovered, and their activities were influenced by interactions. These findings deepen our understanding of the functional and regulatory mechanisms of mito aaRSs.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Medicine, Research & Experimental
Jingdian Zhang, Camilla Koolmeister, Jinming Han, Roberta Filograna, Leo Hanke, Monika Adori, Daniel J. Sheward, Sina Teifel, Shreekara Gopalakrishna, Qiuya Shao, Yong Liu, Keying Zhu, Robert A. Harris, Gerald Mcinerney, Ben Murrell, Mike Aoun, Liselotte Baeckdahl, Rikard Holmdahl, Marcin Pekalski, Anna Wedell, Martin Engvall, Anna Wredenberg, Gunilla B. Karlsson Hedestam, Xaquin Castro Dopico, Joanna Rorbach
Summary: This study investigates the impact of pathogenic mutations in mitochondrial tRNA genes on the immune system. The results show that memory T and B cells have lower mutation burdens compared to their antigen-inexperienced naïve counterparts, and this reduction is less pronounced in myeloid lineages. The rapid dilution of the mutations in T and B cells can be induced by antigen receptor-triggered proliferation and is accelerated under metabolic stress conditions. In addition, the pathogenic mutations affect the metabolic remodeling and IFN-gamma production in CD8(+) T cells.
Article
Biochemistry & Molecular Biology
Gui-Xin Peng, Yong Zhang, Qin-Qin Wang, Qing-Run Li, Hong Xu, En-Duo Wang, Xiao-Long Zhou
Summary: The study revealed the crucial role of GTPBP3 in tRNA modification and its pathogenic mutations affecting structure, function, or localization. Additionally, a novel cytoplasm-localized isoform of hGTPBP3 was identified, suggesting potential noncanonical functions.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Multidisciplinary Sciences
Bernhard Kuhle, Marscha Hirschi, Lili K. K. Doerfel, Gabriel C. C. Lander, Paul Schimmel
Summary: Animal mitochondrial gene expression relies on specific interactions between nuclear-encoded aminoacyl-tRNA synthetases and mitochondria-encoded tRNAs. Their evolution involves an antagonistic interplay between strong mutation pressure on mtRNAs and selection pressure to maintain their essential function. A human mitochondrial seryl-tRNA synthetase and mtRNA(Ser) complex reveals a rewiring of intermolecular recognition rules driven by strong mutation pressure on mtRNA genes.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Rachid Boutoual, Hyunsun Jo, Indra Heckenbach, Ritesh Tiwari, Herbert Kasler, Chad A. Lerner, Samah Shah, Birgit Schilling, Vincenzo Calvanese, Matthew J. Rardin, Morten Scheibye-Knudsen, Eric Verdin
Summary: In this study, a novel mitochondrial protein mt-ELP3 was identified, which was found to regulate the post-transcriptional modification of mitochondrial-transfer RNAs (mt-tRNAs). Overexpression of mt-ELP3 led to increased mitochondrial translation and expression of OXPHOS complexes.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Yan Lin, Xuebi Xu, Wei Wang, Fuchen Liu, Dandan Zhao, Duoling Li, Kunqian Ji, Wei Li, Yuying Zhao, Chuanzhu Yan
Summary: This study identified a mitochondrial tRNA(Ser(UCN)) mutation causing severe mitochondrial myopathy, leading to impaired mitochondrial translation, respiratory deficiency, ROS overproduction, and decreased MMP. These findings offer valuable insights into the pathogenic mechanism and functional consequences of the mt-tRNA mutation.
Article
Cell Biology
Jing Bai, Qiang Ma, Yunfeng Lan, Yating Chen, Shanshan Ma, Jiaxin Li, Chuanbin Liu, Zihao Fu, Xu Lu, Yun Huang, Yang Li
Summary: This study explores how the mitochondrial tRNA 15910 C>T mutation leads to maternally inherited essential hypertension (EH), revealing its significant role in the pathogenesis of the disease. Family members carrying this mutation have a significantly higher incidence of hypertension and mutation-carrying cells exhibit increased viability and proliferative capacity, contributing to the occurrence of EH.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
Yoshitaka Murakami, Fan-Yan Wei, Yoshimi Kawamura, Haruki Horiguchi, Tsuyoshi Kadomatsu, Keishi Miyata, Kyoko Miura, Yuichi Oike, Yukio Ando, Mitsuharu Ueda, Kazuhito Tomizawa, Takeshi Chujo
Summary: A study found that a mitochondrial tRNA modification enzyme, NSUN3, is crucial for embryonic development in mice. Whole-body knockout leads to embryonic death, while heart-specific knockout causes heart aberration in older mice. The research highlights the importance of mitochondrial tRNA anticodon modification for mammalian embryonic development and demonstrates that the loss of a single mitochondrial tRNA modification can induce tissue aberration that worsens with age.
COMMUNICATIONS BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Tianlin Zheng, Qiang Luo, Chengxuan Han, Jiejun Zhou, Jianke Gong, Lei Chun, X. Z. Shawn Xu, Jianfeng Liu
Summary: Reducing the rate of translation can extend lifespan in multiple organisms, but different types of ARS genes play opposing roles in lifespan regulation.
Article
Biochemistry & Molecular Biology
Tingting Yu, Yi Zhang, Wen-Qiang Zheng, Siqi Wu, Guoqiang Li, Yong Zhang, Niu Li, Ruen Yao, Pengfei Fang, Jian Wang, Xiao-Long Zhou
Summary: Mitochondrial translation is crucial for cellular energy homeostasis, and variations in mitochondrial aaRSs can lead to various human diseases. This study identified two novel variants of SARS2 that cause a multisystem disorder. These variants exhibited reduced tRNA binding and aminoacylation capacities, resulting in mitochondrial dysfunction and decreased tRNA abundance due to RNA degradation. The findings highlight the importance of reduced tRNA(Ser)(AGY) abundance in the development of SARS2-related diseases.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Jessica M. Warren, Thalia Salinas-Giege, Deborah A. Triant, Douglas R. Taylor, Laurence Drouard, Daniel B. Sloan
Summary: Research on mitochondrial genomes of Silene plants reveals the process of tRNA gene loss and replacement, as well as the import of nuclear-encoded tRNAs. Even in systems with recent mitochondrial tRNA gene loss, nuclear-encoded counterparts may likely replace mitochondrial tRNAs, providing insight into the evolutionary history of plant mitochondrial genomes.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Review
Biochemistry & Molecular Biology
Cristina Mazzaccara, Bruno Mirra, Ferdinando Barretta, Martina Caiazza, Barbara Lombardo, Olga Scudiero, Nadia Tinto, Giuseppe Limongelli, Giulia Frisso
Summary: Mitochondrial Cardiomyopathy is a common manifestation of multi-organ Mitochondrial Diseases, presenting diagnostic complexities due to clinical and genetic heterogeneity. Molecular screening plays a crucial role in MD management and identifying disease genes can aid in developing targeted gene panels for accurate diagnosis of patients with clinical suspect of mitochondrial cardiomyopathies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
James P. Held, Gaomin Feng, Benjamin R. Saunders, Claudia Pereria, Kristopher Burkewitz, Maulik R. Patel, Xiaochen Wang
Summary: Researchers have discovered a new RNA-based cellular pathway, mediated by the enzyme HOE-1, that activates the mitochondrial unfolded protein response (UPRmt) by regulating the transcription factors ATFS-1 and DVE-1. This pathway does not rely on the integrated stress response and is likely mediated by tRNAs. The subcellular localization of HOE-1 is responsive to mitochondrial stress and is negatively regulated by ATFS-1.
Article
Clinical Neurology
Carolina Croci, Matteo Cataldi, Serena Baratto, Claudio Bruno, Federica Trucco, Stefano Doccini, Alessandro Romano, Claudia Nesti, Filippo Maria Santorelli, Chiara Fiorillo
Summary: This article describes the clinical and genetic characteristics of a patient with PDH deficiency presenting isolated peripheral nervous system involvement. It emphasizes that the diagnosis of PDH deficiency should be considered in children with unexplained peripheral neuropathy, especially in cases of early onset and limited response to treatment. The analysis of lactic acid could help confirm the diagnosis.
Article
Genetics & Heredity
Claudia Dosi, Anna Rubegni, Jacopo Baldacci, Daniele Galatolo, Stefano Doccini, Guja Astrea, Angela Berardinelli, Claudio Bruno, Giorgia Bruno, Giacomo Pietro Comi, Maria Alice Donati, Maria Teresa Dotti, Massimiliano Filosto, Chiara Fiorillo, Fabio Giannini, Gian Luigi Gigli, Marina Grandis, Diego Lopergolo, Francesca Magri, Maria Antonietta Maioli, Alessandro Malandrini, Roberto Massa, Sabrina Mata, Federico Melani, Sonia Messina, Andrea Mignarri, Maurizio Moggio, Elena Maria Pennisi, Elena Pegoraro, Giulia Ricci, Michele Sacchini, Angelo Schenone, Simone Sampaolo, Monica Sciacco, Gabriele Siciliano, Giorgio Tasca, Paola Tonin, Rossella Tupler, Mariarosaria Valente, Nila Volpi, Denise Cassandrini, Filippo Maria Santorelli
Summary: We developed a novel unsupervised cluster analysis method to study 600 patients with suspected inherited myopathy. By integrating genetic, morphological, and clinical data, we identified 4 clusters with distinctive clinical and morphological features among 64 patients with RYR1 variants. This study provides more precise genotype-phenotype correlations for RYR1-related myopathy.
Article
Clinical Neurology
Domenico Giannese, Vincenzo Montano, Piervito Lopriore, Claudia Nesti, Annalisa LoGerfo, Maria Adelaide Caligo, Flavio Dal Canto, Gianandrea Pasquinelli, Angelo Giovanni Bonadio, Diego Moriconi, Gabriele Siciliano, Michelangelo Mancuso
Summary: This article reports a case of a 49-year-old Italian female with encephalomyopathy, chronic proteinuric kidney disease, and a new heteroplasmic m.3274 3275delAC MT-TL1 gene mutation, which has not been described in the literature. Conclusion: This case demonstrates a systemic mitochondrial disease caused by the heteroplasmic m.3274 3275delAC MT-TL1 gene mutation, and a mitochondrial disease should be suspected in case of complex multisystem phenotypes.
JOURNAL OF NEUROMUSCULAR DISEASES
(2023)
Article
Genetics & Heredity
Dmitrijs Rots, Taryn E. Jakub, Crystal Keung, Vissers E. L. M. Lisenka, Siddharth Banka, Rolph Pfundt, Bert B. A. de Vries, Richard H. van Jaarsveld, Saskia M. J. Hopman, Ellen van Binsbergen, Irene Valenzuela, Maja Hempel, Tatjana Bierhals, Fanny Kortuem, Francois Lecoquierre, Alice Goldenberg, Jens Michael Hertz, Charlotte Brasch Andersen, Maria Kibaek, Eloise J. Prijoles, Roger E. Stevenson, David B. Everman, Wesley G. Patterson, Linyan Meng, Charul Gijavanekar, Karl De Dios, Shenela Lakhani, Tess Levy, Matias Wagner, Dagmar Wieczorek, Paul J. Benke, Maria Soledad Lopez Garcia, Renee Perrier, Sergio B. Sousa, Pedro M. Almeida, Maria Jose Simoes, Bertrand Isidor, Wallid Deb, Andrew A. Schmanski, Omar Abdul-Rahman, Christophe Philippe, Ange-Line Bruel, Laurence Faivre, Antonio Vitobello, Christel Thauvin, Jeroen J. Smits, Livia Garavelli, Stefano G. Caraffi, Francesca Peluso, Laura Davis-Keppen, Dylan Platt, Erin Royer, Lisette Leeuwen, Margje Sinnema, Alexander P. A. Stegmann, Constance T. R. M. Stumpel, George E. Tiller, Danielle G. M. Bosch, Stephanus T. Potgieter, Shelagh Joss, Miranda Splitt, Simon Holden, Matina Prapa, Nicola Foulds, Sofia Douzgou, Kaija Puura, Regina Waltes, Andreas G. Chiocchetti, Christine M. Freitag, F. Kyle Satterstrom, Silvia De Rubeis, Joseph Buxbaum, Bruce D. Gelb, Aleksic Branko, Itaru Kushima, Jennifer Howe, Stephen W. Scherer, Alessia Arado, Chiara Baldo, Olivier Patat, Demeer Benedicte, Diego Lopergolo, Filippo M. Santorelli, Tobias B. Haack, Andreas Dufke, Miriam Bertrand, Ruth J. Falb, Angelika Riess, Peter Krieg, Stephanie Spranger, Maria Francesca Bedeschi, Maria Iascone, Sarah Josephi-Taylor, Tony Roscioli, Michael F. Buckley, Jan Liebelt, Aditi I. Dagli, Emmelien Aten, Anna C. E. Hurst, Alesha Hicks, Mohnish Suri, Ermal Aliu, Sunil Naik, Richard Sidlow, Juliette Coursimault, Gael Nicolas, Hanna Kuepper, Florence Petit, Veyan Ibrahim, Deniz Top, Francesca Di Cara, Raymond J. Louie, Elliot Stolerman, Han G. Brunner, Lisenka E. L. M. Vissers, Jamie M. Kramer, Tjitske Kleefstra
Summary: This study examines the clinical and molecular spectrum of individuals with KDM6B variants and challenges the accuracy of the current description of the disorder. Cognitive deficits are consistently observed, but the overall phenotype varies greatly. The study also demonstrates the disruptive effect of certain KDM6B variants on protein structure and introduces a functional testing paradigm for assessing these variants. The findings highlight the importance of international collaboration and rigorous functional analysis in diagnosing rare disorders.
AMERICAN JOURNAL OF HUMAN GENETICS
(2023)
Article
Clinical Neurology
Francesca Sardina, Davide Valente, Gaia Fattorini, Ettore Cioffi, Gianmarco Dalla Zanna, Alessandra Tessa, Daniela Trisciuoglio, Silvia Soddu, Filippo M. Santorelli, Carlo Casali, Cinzia Rinaldo
Summary: The study developed an automated, simple, fast, and non-invasive cell imaging-based method to quantify microtubule cytoskeleton organization changes in lymphoblastoid cells and peripheral blood mononuclear cells. The results showed that individuals affected by SPG4-hereditary spastic paraplegia have a polarized microtubule cytoskeleton organization. The method was able to discriminate SPG4-hereditary spastic paraplegia from healthy donors and other subtypes, and detect the effects of spastin protein level changes.
EUROPEAN JOURNAL OF NEUROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Rosario Licitra, Valentina Naef, Maria Marchese, Devid Damiani, Asahi Ogi, Stefano Doccini, Baldassare Fronte, Jingyu Yan, Filippo M. M. Santorelli
Summary: Milk oligosaccharides, including both sialylated human milk oligosaccharides and bovine-derived oligosaccharides, have been found to support brain proliferation and maturation in zebrafish. Early nutrition plays a role in modulating nervous system development and may lead to epigenetic imprinting. The treatments of increasing sialylated oligosaccharide content did not show significant effects on mortality rates, locomotor behavior, and gene expression, but did result in increased test plate exploration in the dark.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Emanuele Monda, Michele Lioncino, Martina Caiazza, Vincenzo Simonelli, Claudia Nesti, Marta Rubino, Alessia Perna, Alfredo Mauriello, Alberta Budillon, Vincenzo Pota, Giorgia Bruno, Antonio Varone, Vincenzo Nigro, Filippo Maria Santorelli, Giuseppe Pacileo, Maria Giovanna Russo, Giulia Frisso, Simone Sampaolo, Giuseppe Limongelli
Summary: This study describes a group of patients with cardiomyopathy caused by neuromuscular diseases or mitochondrial diseases. The clinical phenotype of these patients was described through comprehensive clinical, molecular, and histological characteristics. A multidisciplinary evaluation and genetic testing play a key role in diagnosing these rare diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Letter
Clinical Neurology
Claudia Nesti, Chiara Ticci, Anna Rubegni, Stefano Doccini, Giusi Scaturro, Annalisa Vetro, Renzo Guerrini, Filippo M. Santorelli, Elena Procopio
JOURNAL OF NEUROLOGY
(2023)
Letter
Cardiac & Cardiovascular Systems
Michele Lioncino, Emanuele Monda, Martina Caiazza, Vincenzo Simonelli, Claudia Nesti, Alfredo Mauriello, Alberta Budillon, Alessandro Di Santo, Giorgia Bruno, Antonio Varone, Vincenzo Nigro, Filippo Maria Santorelli, Giuseppe Pacileo, Maria Giovanna Russo, Giulia Frisso, Simone Sampaolo, Giuseppe Limongelli
CIRCULATION-GENOMIC AND PRECISION MEDICINE
(2023)
Review
Clinical Neurology
Deborah Leccese, Gabriele Rosario Rodolico, Martina Sperti, Denise Cassandrini, Marco Bartolini, Assunta Ingannato, Benedetta Nacmias, Laura Bracco, Alessandro Malandrini, Filippo Maria Santorelli, Valentina Bessi, Sabrina Mata
Summary: This study reports a novel VCP missense mutation in an Italian family with FTD and compares the findings with literature. The mutation was found in all affected family members and is associated with progressive muscle weakness, cognitive disorders, and bone abnormalities.
CLINICAL NEUROLOGY AND NEUROSURGERY
(2023)
Article
Biochemistry & Molecular Biology
Maria Caputo, Ilaria Martinelli, Nicola Fini, Giulia Gianferrari, Cecilia Simonini, Rosanna Trovato, Filippo Maria Santorelli, Alessandra Tessa, Jessica Mandrioli, Elisabetta Zucchi
Summary: Mutations in the TBCD gene lead to neurodevelopment regression, epilepsy, and mental retardation. We report a rare homozygous variant in TBCD, causing distal motorneuronopathy and mild mental retardation, with evidence of corpus callosum hypoplasia on neuroimaging. The molecular interpretation of this unique phenotype expands the literature on tubulinopathies associated with TBCD mutations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Sara Carpi, Stefano Quarta, Stefano Doccini, Anella Saviano, Noemi Marigliano, Beatrice Polini, Marika Massaro, Maria Annunziata Carluccio, Nadia Calabriso, Martin Wabitsch, Filippo Maria Santorelli, Marco Cecchini, Francesco Maione, Paola Nieri, Egeria Scoditti
Summary: The two compounds TIIA and CRY found in Danshen were found to reduce inflammation in adipocytes and prevent inflammatory response through modulation of gene and miRNA expression. This offers a new approach for preventing and treating obesity-related diseases.
Meeting Abstract
Clinical Neurology
Isabella Di Sarno, Stefano Tozza, Filippo Maria Santorelli, Rosa Iodice, Raffaele Dubbioso, Lucia Ruggiero, Maria Nolano, Fiore Manganelli
JOURNAL OF THE PERIPHERAL NERVOUS SYSTEM
(2023)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)