Article
Biology
ShylajaNaciyar Mohandass, Mangalalakshmi Ragavan, Dineshbabu Gnanasekaran, Uma Lakshmanan, Prabaharan Dharmar, Sushanta Kumar Saha
Summary: Synthetic azo dyes are widely used but their improper discharge poses a threat to the environment. Genetically modified cyanobacteria with enhanced H2O2 accumulation through SOD overexpression can effectively degrade these dyes, suggesting a valuable bioremediation strategy.
Article
Cell Biology
Dong-Dong Wu, Sheng Jin, Ruo-Xiao Cheng, Wen-Jie Cai, Wen-Long Xue, Qing-Qing Zhang, Le-Jie Yang, Qi Zhu, Meng-Yao Li, Ge Lin, Yi-Zhen Wang, Xue-Pan Mu, Yu Wang, Igor Ying Zhang, Qi Zhang, Ying Chen, Sheng-Yang Cai, Bo Tan, Ye Li, Yun-Qian Chen, Pu-Juan Zhang, Chen Sun, Yue Yin, Ming-Jie Wang, Yi-Zhun Zhu, Bei-Bei Tao, Jia-Hai Zhou, Wei-Xue Huang, Yi-Chun Zhu
Summary: This study investigates the possibility of a mechanism beyond post-translational modifications to regulate protein function. It shows that hydrogen sulfide (H2S) can bind to the active-site copper of Cu/Zn-SOD, enhancing electrostatic forces and facilitating electron transfer. The physiological relevance of this H2S effect is demonstrated in in vitro and in vivo models where the cardioprotective effects of H2S depend on Cu/Zn-SOD.
Article
Nanoscience & Nanotechnology
Xiangshi Sun, Kongtong Yu, Yulin Zhou, Shiyan Dong, Wenji Hu, Yating Sun, Yuhuan Li, Jing Xie, Robert J. Lee, Fengying Sun, Yifan Ma, Shengnian Wang, Betty Y. S. Kim, Yifan Wang, Zhaogang Yang, Wen Jiang, Youxin Li, Lesheng Teng
Summary: The newly synthesized amphiphilic polymers can self-assemble into micellar nanoparticles with favorable hydrolysis kinetics, biocompatibility, and long circulation time. These materials not only exhibit better inflammation-targeting effects, but also do not induce an acidic microenvironment during degradation.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Nutrition & Dietetics
Mariangela Rondanelli, Niccolo Miraglia, Pietro Putignano, Ignazio Castagliuolo, Paola Brun, Stefano Dall'Acqua, Gabriella Peroni, Milena Anna Faliva, Maurizio Naso, Mara Nichetti, Vittoria Infantino, Simone Perna
Summary: The study showed that supplementation with Saccharomyces boulardii and Superoxide Dismutase led to significant weight loss and reduction in fat mass in obese adults, while maintaining fat free mass. Additionally, a decrease in HOMA index and uric acid levels were observed, providing added benefits in obesity management. Moreover, there was a significant increase in Vitamin D levels in the intervention group, which warrants further investigation.
Article
Biochemistry & Molecular Biology
Bindu Chandrasekharan, Claudia Montllor-Albalate, Alyson E. Colin, Joshua L. Andersen, Young C. Jang, Amit R. Reddi
Summary: Cu/Zn superoxide dismutase (Sod1) catalyzes the breakdown of superoxide radicals into oxygen and hydrogen peroxide, which acts as a signaling molecule. In yeast, Sod1 is involved in a redox signaling pathway that connects nutrient availability to energy metabolism control. This pathway stabilizes plasma membrane kinases Yck1/2, promoting glycolytic growth and repressing respiration.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Chemistry, Multidisciplinary
Namrata Singh, G. R. Sherin, Govindasamy Mugesh
Summary: Nanozymes, nanomaterials with enzyme-mimicking activity, have attracted significant interest in biomedical applications, particularly in oxidative stress-mediated disorders. The challenge lies in designing nanozymes that can selectively differentiate between physiological oxidative stress and pathological oxidative stress and mediate appropriate redox reactions in cells, tissues, and organs. This review summarizes the advancements in redox-active nanozymes and their therapeutic significance in various disease models, such as cancer, neurodegeneration, and cardiovascular diseases. It also discusses the future prospects and challenges associated with the biomedical applications of nanozymes.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Ahmet Can Timucin, Suleyman Selim Cinaroglu, Osman Ugur Sezerman, Emel Timucin
Summary: The metallation status of human Cu/Zn superoxide dismutase 1 (SOD1) is crucial in the development of amyotrophic lateral sclerosis (ALS). Mutations at the ALS-linked positions H63R and K136A significantly affect dimer dynamics, leading to demetallation and potential ALS toxicity. This study highlights the potential use of atomistic simulations in studying disease variants related to SOD1.
FRONTIERS IN CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Phitsanu Pinmanee, Kamonwan Sompinit, Jantima Arnthong, Surisa Suwannarangsee, Angkana Jantimaporn, Mattaka Khongkow, Thidarat Nimchua, Prakit Sukyai
Summary: Superoxide dismutase (SOD), an antioxidant enzyme, plays a crucial role in protecting cells against reactive oxygen species (ROS). In this study, the high SOD production of Saccharomyces cerevisiae TBRC657 was utilized. By optimizing the culture medium, molasses and yeast extract were identified as the major components that significantly increased SOD production. The optimized medium did not require induction, making it suitable for large-scale production. The SOD formulation, with trehalose as the best additive, exhibited enhanced stability and maintained activity after storage. The obtained SOD demonstrated cytotoxicity and ROS elimination properties in fibroblast cells. Therefore, S. cerevisiae TBRC657-derived SOD in the optimized medium could be a potential ROS scavenger for various industries.
FERMENTATION-BASEL
(2022)
Article
Plant Sciences
Li Tian, Junjiao Li, Caimin Huang, Dandan Zhang, Yan Xu, Xingyong Yang, Jian Song, Dan Wang, Nianwei Qiu, Dylan P. G. Short, Patrik Inderbitzin, Krishna Subbarao, Jieyin Chen, Xiaofeng Dai
Summary: The accumulation of ROS is a common defense mechanism in higher plants against pathogen attack. Previous studies showed that Cu/Zn-SOD1 (VdSOD1) in plants is upregulated following infection, indicating its role in pathogen virulence. Deletion of VdSOD1 leads to increased sensitivity to ROS generator menadione and relies on Golgi reassembly stacking protein for secretion. Overall, VdSOD1 plays a significant role in scavenging ROS and promoting virulence in V. dahliae.
MOLECULAR PLANT PATHOLOGY
(2021)
Article
Fisheries
Alfredo Loor, Dongdong Wang, Peter Bossier, Nancy Nevejan
Summary: This study examines the immunostimulatory capacity of yeast cells in bivalves and finds that the cell wall components play a crucial role in immune activation. Feeding bivalves with feed containing Amnn9 can significantly enhance their resistance against bacterial infections.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
William M. Shaw, Lucie Studena, Kyler Roy, Piotr Hapeta, Nicholas S. McCarty, Alicia E. Graham, Tom Ellis, Rodrigo Ledesma-Amaro
Summary: CRISPR gene activation and inhibition is a powerful synthetic tool for influencing gene expression, with applications in foundational studies, cellular reprogramming, and metabolic engineering. The authors developed a method for inducible expression of multiple gRNAs, increasing the capacity for CRISPR gene activation and inhibition and enhancing flexibility in target gene manipulation. They also created a highly regulated and user-friendly CRISPR toolkit in yeast, enabling simultaneous activation and repression of 11 genes in central metabolism, resulting in a significant increase in succinic acid production.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Francisco Javier Martinez-Noguera, Pedro E. Alcaraz, Raquel Ortolano-Rios, Stephane P. Dufour, Cristian Marin-Pagan
Summary: This study compared the differences in endogenous antioxidant enzymes and hemogram between professional and amateur cyclists, finding higher values in CAT, GSSG, and GSSG/GSH ratio and lower values in SOD in the professional group. There was an inverse correlation between power produced at ventilation thresholds 1 and 2 and GSSG/GSH ratio in the professional group, suggesting a relationship between these parameters and moderate and submaximal exercise performance.
Article
Plant Sciences
Yueyun Pan, Shousong Zhu, Na Song, Pengyu Hou, Xinyi Yu, Jian Zang, Xiaolei Niu, Honggang Wang, Yinhua Chen
Summary: The study found that the antioxidant enzyme Cu/Zn-SOD gene discovered in Ceriops tagal significantly improves salt tolerance in yeast.
PAKISTAN JOURNAL OF BOTANY
(2021)
Article
Chemistry, Multidisciplinary
Wentao Wang, Yumeng Gao, Wang Xu, Yan Xu, Ninglin Zhou, Yuanyuan Li, Ming Zhang, Ben Zhong Tang
Summary: This study presents a new approach for treating chronic wounds caused by bacterial infections. By using nanoparticles, this method can simultaneously scavenge inflammation, alleviate hypoxia conditions, and promote the penetration of therapeutic agents in wound tissues. Experimental results showed that this approach inhibits bacterial activities and its effectiveness was demonstrated in animal experiments.
ADVANCED MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Jose Teixeira, Farhan Basit, Peter H. G. M. Willems, Jori A. Wagenaars, Els van de Westerlo, Ricardo Amorim, Fernando Cagide, Sofia Benfeito, Catarina Oliveira, Fernanda Borges, Paulo J. Oliveira, Werner J. H. Koopman
Summary: The novel mitochondria-targeted variants of gallic and caffeic acid were found to increase ROS levels, stimulate NRF2 expression, and increase SOD2 and GSH levels.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)