Article
Immunology
Nicolas Krause, Joerg Mengwasser, Elpida Phithak, Francisca Beato, Marc Appis, Edgar Louis Milford, Johan Pratschke, Igor Sauer, Anja Kuehl, Arndt Vogel, Michael Goodyear, Linda Hammerich, Frank Tacke, Johanna Faith Haas, Tobias Mueller, Nalan Utku
Summary: IR1 cells are a subset of T regulatory cells that have immune suppressive functions and can inhibit proliferation in mixed lymphocyte reactions. They deliver regulatory signals by binding to their ligand, HLA-DR alpha 2, and have the potential to modulate immune dysregulation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Yue Zhang, Yan Yan, Jingjing Meng, Mohit Girotra, Sundaram Ramakrishnan, Sabita Roy
Summary: Extracellular vesicles (EVs) serve as effective mediators of intercellular communication between enterocytes and immune cells, modulating inflammatory responses through microRNA, especially Let-7. Studies using in vitro and murine models demonstrated that EVs derived from intestinal organoids can reduce inflammation and alleviate colitis symptoms, with opioid use exacerbating immune responses in inflammatory diseases.
MUCOSAL IMMUNOLOGY
(2021)
Review
Immunology
Yuzhi Lu, Ni Xia, Xiang Cheng
Summary: Regulatory T cells (Tregs) play a crucial role in the development of chronic heart failure, suppressing excessive inflammatory responses and promoting stable scar formation in the early stage of heart injury. However, in chronic heart failure, the functions and phenotypes of Tregs change, transforming into a cell type that is harmful to the heart.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Ke Jin, Simon Parreau, Kenneth J. Warrington, Matthew J. Koster, Gerald J. Berry, Joerg J. Goronzy, Cornelia M. Weyand
Summary: Vasculitides, a group of diseases causing vascular damage, result in loss of immune privilege and uncontrolled inflammation. Deficiencies in immunosuppressive regulatory T cells (Treg) play a crucial role, with different vasculitides being affected by distinct mechanisms. Exploring the mechanisms behind insufficient immunosuppression and vascular inflammation holds promise for novel therapeutic interventions.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Immunology
Meihong Da, Luxia Chen, Alexander Enk, Sabine Ring, Karsten Mahnke
Summary: Adenosine (Ado) has immunosuppressive effects, and the ectoenzyme CD73 is crucial for the production of extracellular Ado. CD73 is highly expressed in regulatory T cells (Tregs) and upregulated after activation. Ado generated by CD73(+) Tregs plays a role in immune suppression of dendritic cells, monocytes, and T cells, making CD73 a novel checkpoint inhibitor in immunosuppressive environments such as tumors. Therapeutic intervention targeting CD73 is currently under investigation.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Immunology
Paola de Candia, Claudio Procaccini, Claudia Russo, Maria Teresa Lepore, Giuseppe Matarese
Summary: Regulatory T cells, as key metabolic sensors, play a crucial role in controlling immune state and have promising therapeutic potential for autoimmune and metabolic disorders.
Review
Biochemistry & Molecular Biology
Natalia M. Krajewska, Remi Fiancette, Ye H. Oo
Summary: Immune-mediated cholangiopathies are characterized by the destruction of bile ducts, leading to liver inflammation and fibrosis. Regulatory T cells (Tregs) play a crucial role in these diseases, but their function can be impaired by the inflamed microenvironment. Mast cells (MCs) are also involved in the pathogenesis by releasing pro-inflammatory mediators. MCs can indirectly influence Treg function and there are also direct interactions between MCs and Tregs. Understanding the crosstalk between Tregs and MCs is important for the progression of autoimmune cholangiopathy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Immunology
Qi Jiang, Guocan Yang, Qi Liu, Shengjun Wang, Dawei Cui
Summary: Rheumatoid arthritis is a systemic and heterogeneous autoimmune disease characterized by symmetrical polyarthritis, with dysfunction of regulatory T (Treg) cells potentially contributing to the breakdown of self-tolerance. The ideal treatment strategy for RA should focus on re-inducing self-tolerance to prevent obvious tissue injury.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Lourdes Rocamora-Reverte, Franz Leonard Melzer, Reinhard Wuerzner, Birgit Weinberger
Summary: The immune system is a tightly regulated network, where regulatory T cells play an important role in maintaining immune homeostasis. Different types of T cells have regulatory functions in modulating immune responses, and the aging process can affect the composition of immune cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Nutrition & Dietetics
Natalia Diaz-Garrido, Josefa Badia, Laura Baldoma
Summary: This study aimed to understand the mechanisms of extracellular vesicles (BEVs) in driving specific immune responses. The production of cytokines and exosomes by dendritic cells (DCs) challenged with BEVs from different strains of E. coli was analyzed. The results showed that the strains had differential effects on the production of specific cytokines and the composition of immune-related miRNA in the exosomes.
Review
Immunology
Jingli Lu, Yan Liang, Haiyang Meng, Ailing Zhang, Junjie Zhao, Chengliang Zhang
Summary: The article discusses the metabolic and epigenetic features of Treg cells, as well as the impact of metabolites on epigenetic changes in Treg cells, which in turn affect the activation, differentiation, and function of Treg cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Yiran Qiu, Shouyu Ke, Jieqiong Chen, Zhizhen Qin, Wenle Zhang, Yaqin Yuan, Dehua Meng, Gang Zhao, Kejin Wu, Bin Li, Dan Li
Summary: This review summarizes the latest findings on the dynamic connections and reciprocal regulations of non-lymphoid Treg cell subsets in the immune escape of tumour cells in solid tumors, providing new insights for the development of next-generation engineered T cell-based immune treatments for solid tumors.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Immunology
Jaehwan Kim, Ariana Moreno, James G. G. Krueger
Summary: Psoriasis vulgaris is a common inflammatory disease affecting 7.5 million adults in the US, with immunopathogenesis paradigms rapidly evolving due to advancements in technology. Single-cell RNA sequencing technology is now being applied to clinical trials of biologics to test the expansion of regulatory immune cell subsets involved in skin homeostasis.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Immunology
Jing Lu, Taotao Liang, Ping Li, Qingsong Yin
Summary: The tumor microenvironment (TME) plays a crucial role in various aspects of tumor progression and clinical outcomes. Understanding the status of TME, the involvement of immune cells, and the key factors driving T cell dysfunction is essential for developing effective therapies. This study focuses on the regulatory effects of Interferon regulatory factor 4 (IRF4) on immune cells in the TME and highlights its potential as a therapeutic target for reversing T cell exhaustion and promoting anti-tumor immunity.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yuichiro Nakata, Kento Miura, Norimasa Yamasaki, Sawako Ogata, Shuka Miura, Naohisa Hosomi, Osamu Kaminuma
Summary: This study investigated the expression and function of nicotinic acetylcholine receptors (nAChRs) in murine-induced regulatory T (Treg) cells. The results showed that nicotine suppressed the production of transforming growth factor-beta 1 (TGF-β 1) in iTreg cells, but did not affect interleukin (IL)-10 production and proliferation. Different subtypes of nAChRs were expressed in different T-cell subsets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Adam G. Laing, Giorgia Fanelli, Andrei Ramirez-Valdez, Robert I. Lechler, Giovanna Lombardi, Paul T. Sharpe
Article
Immunology
Dominic A. Boardman, Rosa Garcia, Sabine M. Ivison, Brian Bressler, T. G. Murali Dhar, Qihong Zhao, Megan K. Levings
EUROPEAN JOURNAL OF IMMUNOLOGY
(2020)
Article
Cell Biology
Sim Lai Tung, Giorgia Fanelli, Robert Ian Matthews, Jordan Bazoer, Marilena Letizia, Gema Vizcay-Barrena, Farid N. Faruqu, Christina Philippeos, Rosalind Hannen, Khuloud T. Al-Jamal, Giovanna Lombardi, Lesley Ann Smyth
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Review
Medicine, Research & Experimental
Anastasia Polycarpou, Mark Howard, Conrad A. Farrar, Roseanna Greenlaw, Giorgia Fanelli, Russell Wallis, Linda S. Klavinskis, Steven Sacks
EMBO MOLECULAR MEDICINE
(2020)
Article
Biochemical Research Methods
Dan Wu, May Q. Wong, Jens Vent-Schmidt, Dominic A. Boardman, Theodore S. Steiner, Megan K. Levings
Summary: This study describes a new method for purifying, expanding, and retrovirally transducing mouse Tregs, and confirms the feasibility of the method in experiments. By using this method, it is possible to study transduced Tregs in animal models, providing a new avenue for research on genetically engineered Treg therapy for various inflammatory diseases.
JOURNAL OF IMMUNOLOGICAL METHODS
(2021)
Article
Immunology
James L. Reading, Valerie D. Roobrouck, Caroline M. Hull, Pablo Daniel Becker, Jelle Beyens, Alice Valentin-Torres, Dominic Boardman, Estefania Nova Lamperti, Samantha Stubblefield, Giovanna Lombardi, Robert Deans, Anthony E. Ting, Timothy Tree
Summary: Recent clinical experience has shown that using MAPC cell co-culture can enhance the ex vivo expansion efficiency of Treg cells, reducing manufacturing time and increasing yield. MulTreg cells are easier to expand in patients with autoimmune diseases, indicating potential clinical utility.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Andrea Cossarizza, Hyun-Dong Chang, Andreas Radbruch, Sergio Abrignani, Richard Addo, Muebeccel Akdis, Immanuel Andrae, Francesco Andreata, Francesco Annunziato, Eduardo Arranz, Petra Bacher, Sudipto Bari, Vincenzo Barnaba, Joana Barros-Martins, Dirk Baumjohann, Cristian G. Beccaria, David Bernardo, Dominic A. Boardman, Jessica Borger, Chotima Boettcher, Leonie Brockmann, Marie Burns, Dirk H. Busch, Garth Cameron, Ilenia Cammarata, Antonino Cassotta, Yinshui Chang, Fernando Gabriel Chirdo, Eleni Christakou, Luka Cicin-Sain, Laura Cook, Alexandra J. Corbett, Rebecca Cornelis, Lorenzo Cosmi, Martin S. Davey, Sara De Biasi, Gabriele De Simone, Genny del Zotto, Michael Delacher, Francesca Di Rosa, James Di Santo, Andreas Diefenbach, Jun Dong, Thomas Doerner, Regine J. Dress, Charles-Antoine Dutertre, Sidonia B. G. Eckle, Pascale Eede, Maximilien Evrard, Christine S. Falk, Markus Feuerer, Simon Fillatreau, Aida Fiz-Lopez, Marie Follo, Gemma A. Foulds, Julia Froebel, Nicola Gagliani, Giovanni Galletti, Anastasia Gangaev, Natalio Garbi, Jose Antonio Garrote, Jens Geginat, Nicholas A. Gherardin, Lara Gibellini, Florent Ginhoux, Dale I. Godfrey, Paola Gruarin, Claudia Haftmann, Leo Hansmann, Christopher M. Harpur, Adrian C. Hayday, Guido Heine, Daniela Carolina Hernandez, Martin Herrmann, Oliver Hoelsken, Qing Huang, Samuel Huber, Johanna E. Huber, Jochen Huehn, Michael Hundemer, William Y. K. Hwang, Matteo Iannacone, Sabine M. Ivison, Hans-Martin Jaeck, Peter K. Jani, Baerbel Keller, Nina Kessler, Steven Ketelaars, Laura Knop, Jasmin Knopf, Hui-Fern Koay, Katja Kobow, Katharina Kriegsmann, H. Kristyanto, Andreas Krueger, Jenny F. Kuehne, Heike Kunze-Schumacher, Pia Kvistborg, Immanuel Kwok, Daniela Latorre, Daniel Lenz, Megan K. Levings, Andreia C. Lino, Francesco Liotta, Heather M. Long, Enrico Lugli, Katherine N. MacDonald, Laura Maggi, Mala K. Maini, Florian Mair, Calin Manta, Rudolf Armin Manz, Mir-Farzin Mashreghi, Alessio Mazzoni, James McCluskey, Henrik E. Mei, Fritz Melchers, Susanne Melzer, Dirk Mielenz, Leticia Monin, Lorenzo Moretta, Gabriele Multhoff, Luis Enrique Munoz, Miguel Munoz-Ruiz, Franziska Muscate, Ambra Natalini, Katrin Neumann, Lai Guan Ng, Antonia Niedobitek, Jana Niemz, Larissa Nogueira Almeida, Samuele Notarbartolo, Lennard Ostendorf, Laura J. Pallett, Amit A. Patel, Gulce Itir Percin, Giovanna Peruzzi, Marcello Pinti, A. Graham Pockley, Katharina Pracht, Immo Prinz, Irma Pujol-Autonell, Nadia Pulvirenti, Linda Quatrini, Kylie M. Quinn, Helena Radbruch, Hefin Rhys, Maria B. Rodrigo, Chiara Romagnani, Carina Saggau, Shimon Sakaguchi, Federica Sallusto, Lieke Sanderink, Inga Sandrock, Christine Schauer, Alexander Scheffold, Hans U. Scherer, Matthias Schiemann, Frank A. Schildberg, Kilian Schober, Janina Schoen, Wolfgang Schuh, Thomas Schueler, Axel R. Schulz, Sebastian Schulz, Julia Schulze, Sonia Simonetti, Jeeshan Singh, Katarzyna M. Sitnik, Regina Stark, Sarah Starossom, Christina Stehle, Franziska Szelinski, Leonard Tan, Attila Tarnok, Julia Tornack, Timothy I. M. Tree, Jasper J. P. van Beek, Willem van de Veen, Klaas van Gisbergen, Chiara Vasco, Nikita A. Verheyden, Anouk von Borstel, Kirsten A. Ward-Hartstonge, Klaus Warnatz, Claudia Waskow, Annika Wiedemann, Anneke Wilharm, James Wing, Oliver Wirz, Jens Wittner, Jennie H. M. Yang, Juhao Yang
Summary: The third edition of Flow Cytometry Guidelines provides comprehensive information on phenotypes and functional assays of major human and murine immune cell subsets, as well as clinical sample analysis and applications in various diseases. It also includes expert tips, tricks, and pitfalls to avoid, making it an essential handbook for researchers.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Review
Allergy
Dominic A. Boardman, Megan K. Levings
Summary: This article reviews the application of gene editing technologies in Treg cell therapy, focusing on preclinical strategies to enhance the efficacy, function, and survival of Treg cells. It explores methods for generating immunoregulatory cells ex vivo, discusses emerging strategies such as using chimeric antigen receptors for antigen specificity, and outlines concepts for repurposing conventional T cells to target and destroy autoreactive and alloreactive lymphocytes. The article also discusses the key obstacles currently limiting the clinical adoption of Treg cell therapy and proposes potential future research directions.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Article
Cell Biology
Marco Romano, Raul Elgueta, Daniel McCluskey, Ana Maria Ortega-Prieto, Emilie Stolarczyk, Francesco Dazzi, Baltasar Lucendo-Villarin, Jose Meseguer-Ripolles, James Williams, Giorgia Fanelli, David C. Hay, Fiona M. Watt, Giovanna Lombardi
Summary: Regenerative medicine aims to replace damaged tissues by stimulating tissue repair or transplanting cells. Pluripotent stem cells have the potential for unlimited cell production, making them valuable for regenerative medicine. However, immune responses pose a challenge to success. This study investigates the immunological properties of human pluripotent stem cell-derived hepatocyte-like cells and shows their potential for in vitro immunoregulation.
Article
Immunology
Dominic A. Boardman, May Q. Wong, William D. Rees, Dan Wu, Megan E. Himmel, Paul C. Orban, Jens Vent-Schmidt, Nicholas C. Zachos, Theodore S. Steiner, Megan K. Levings
Summary: Regulatory T cell (Treg) therapy is a promising strategy for treating inflammatory bowel disease (IBD). A novel chimeric antigen receptor (CAR) specific for flagellin derived from Escherichia coli H18 (FliC) was developed to confer FliC-specificity to human Tregs. FliC-CAR Tregs showed specificity and functionality, and demonstrated potential in treating IBD by suppressing inflammation and promoting the establishment of colon-derived epithelial cell monolayers.
JOURNAL OF AUTOIMMUNITY
(2023)
Editorial Material
Surgery
Manjurul Haque, Dominic A. Boardman, Megan K. Levings
AMERICAN JOURNAL OF TRANSPLANTATION
(2023)
Article
Cell Biology
Giorgia Fanelli, Marco Romano, Giovanna Lombardi, Steven H. Sacks
Summary: Retinal pigment epithelium (RPE) cell allotransplantation is a potential solution for retinal diseases. However, the RPE-complement system triggered by CL-11 could hinder the success of transplantation due to the inflammatory response and T cell recognition promotion. We found that CL-11 inhibited T cell proliferation by binding to T cells and downregulating CD28 expression. Additionally, soluble CL-11 secreted by stressed RPE cells may contribute to the immunosuppressive properties of RPE cells.
Article
Biology
Manjurul Haque, Dominic A. Boardman, Avery J. Lam, Katherine N. MacDonald, Lieke Sanderink, Qing Huang, Vivian C. W. Fung, Sabine Ivison, Majid Mojibian, Megan K. Levings
Summary: Graft-versus-host disease (GvHD) is a significant complication of allogeneic hematopoietic stem cell transplantation, and there is a need to recapitulate its effects in preclinical, in vivo systems. Humanized mouse models of GvHD are commonly used for this purpose, where mice are reconstituted with human immune cells and develop a condition known as xenoGvHD. This study provides detailed methods for inducing xenoGvHD in immunodeficient mice using human peripheral blood mononuclear cells, and also includes comprehensive instructions for T cell preparation, mouse monitoring, data collection, interpretation, and reporting. The protocol presented here aims to standardize the xenoGvHD model and facilitate comparison of data across different studies.
Article
Biology
Qing Huang, Avery J. Lam, Dominic A. Boardman, Nicholas A. J. Dawson, Megan K. Levings
Summary: Regulatory T cells (Tregs) can suppress immune responses through various mechanisms and are used as cellular therapy to induce tolerance. This protocol describes a method to measure the suppression of CD80 and CD86 expression on dendritic cells by human Tregs, providing a way to assess their functional activity.
Review
Immunology
Marco Romano, Giorgia Fanelli, Caraugh Jane Albany, Giulio Giganti, Giovanna Lombardi
FRONTIERS IN IMMUNOLOGY
(2019)
