Article
Immunology
Zhiguang Wu, Barbara Shih, Joni Macdonald, Dominique Meunier, Kris Hogan, Cosmin Chintoan-Uta, Hazel Gilhooley, Tuanjun Hu, Mariana Beltran, Neil C. Henderson, Helen M. Sang, Mark P. Stevens, Michael J. Mcgrew, Adam Balic
Summary: This study characterized chicken splenic conventional dendritic cells (cDCs) using a gene-edited chicken line and single-cell RNA sequencing (scRNAseq). Unlike mammals, the chicken spleen contains a single cDC subset expressing chemokine receptor XCR1. The mature XCR1(+) cDCs in the chicken spleen are closely associated with CD8(+) T-cells, suggesting a conserved role in driving CD8(+) T-cell responses.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Medicine, Research & Experimental
Tingting Zhang, Adila Aipire, Yijie Li, Changying Guo, Jinyao Li
Summary: This review summarizes the mechanisms of cross-presentation (XPT) and its application in tumor immunotherapy. Dendritic cells enhance their antigen presentation capacity through receptor-mediated internalization of exogenous antigens, endosome escape, engagement of other XPT-related proteins, and the use of adjuvants, thereby improving the outcomes of DC-based therapy.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Immunology
Gaetan Barbet, Priyanka Nair-Gupta, Michael Schotsaert, Stephen T. Yeung, Julien Moretti, Fabian Seyffer, Giorgi Metreveli, Thomas Gardner, Angela Choi, Domenico Tortorella, Robert Tampe, Kamal M. Khanna, Adolfo Garcia-Sastre, J. Magarian Blander
Summary: The study explains how viruses target host cell antigen-processing pathways, focusing on MHC-I molecules and the re-routing of ERGIC-resident MHC molecules to phagosomal vesicles for cross-presentation. It suggests that even without TAP, protective CD8(+) T cells can still be mobilized during viral infection, highlighting a noncanonical cross-presentation pathway.
Article
Immunology
Demo Yemane Tesfaye, Sonja Bobic, Anna Lysen, Peter Csaba Huszthy, Arnar Gudjonsson, Ranveig Braathen, Bjarne Bogen, Even Fossum
Summary: Targeting antigen to cDC1s can enhance immune responses and influence immune polarization. Xcl1-fusion vaccines induce IgG2a/IgG2b antibody responses and rapid Th1 cell polarization, while fliC-fusion vaccines mainly induce IgG1 antibody response. Induction of IgG2a-dominated responses is important for optimal protection.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Immunology
Christophe Macri, Devi Jenika, Cassandra Ouslinis, Justine D. Mintern
Summary: Dendritic cells (DCs) are specialized antigen-presenting cells that play a critical role in adaptive immunity. Targeting antigen directly to DCs is a selective vaccination strategy that takes advantage of the antigen uptake and presentation functions of DC subsets. This review focuses on DC-targeted vaccination strategies aimed at inducing effective cross-presentation for CD8+ T cell immunity. Receptors highly expressed by mouse and human cDCs, such as DEC205, Clec9A, and XCR1, are explored. The outcomes of DC-targeted vaccination in mouse models and human clinical trials are discussed, highlighting its potential for the prevention and treatment of tumors and infectious diseases.
SEMINARS IN IMMUNOLOGY
(2023)
Article
Immunology
Diego Del Balzo, Anahi Capmany, Ignacio Cebrian, Maria Teresa Damiani
Summary: This study reveals that Chlamydia trachomatis can induce productive infections in murine dendritic cells and disrupt MHC-I trafficking, leading to impaired antigen cross-presentation ability.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Hussein Hamade, Jasmine T. Stamps, Dalton T. Stamps, Shyam K. More, Lisa S. Thomas, Anna Y. Blackwood, Nawele L. Lahcene, Sofi L. Castanon, Brenda C. Salumbides, Yosuke Shimodaira, Helen S. Goodridge, Stephan R. Targan, Kathrin S. Michelsen
Summary: Intestinal dendritic cells play an important role in metabolic syndrome by preserving beneficial microbiota and maintaining intestinal epithelial barrier function.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Paul Noe, Joy H. Wang, Kyu Chung, Zhiyong Cheng, Jessica J. Field, Xiaomeng Shen, Christa L. Cortesio, Cinthia V. Pastuskovas, Hyewon Phee, Kristin V. Tarbell, Jackson G. Egen, Amy-Jo Casbon
Summary: Targeting IFN directly to cDC1s can activate anti-tumor immunity, but the formation of drug antibodies (ADA) remains a major hurdle.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
J. Grabowska, A. J. Affandi, D. van Dinther, M. K. Nijen Twilhaar, K. Olesek, L. Hoogterp, M. Ambrosini, D. A. M. Heijnen, L. Klaase, A. Hidalgo, K. Asano, P. R. Crocker, G. Storm, Y. van Kooyk, J. M. M. den Haan
Summary: In this study, liposomes containing GM3 were shown to efficiently target CD169(+) macrophages, inducing specific immune responses including antigen-specific CD8(+) and CD4(+) T cell as well as B cell responses. Administration of GM3 liposomes containing ovalbumin resulted in delayed tumor growth and improved survival in B16-OVA tumor bearing mice. The study also highlighted the essential role of CD169(+) macrophages and cDC1s in inducing CD8(+) T cell immunity through liposomal vaccination.
JOURNAL OF CONTROLLED RELEASE
(2021)
Review
Oncology
Robin Reschke, Daniel J. Olson
Summary: The lack of T cells in tumors is one of the main reasons for the failure of immunotherapy. Strengthening innate immune mechanisms can improve the efficacy of tumor immunotherapy, such as delivering innate immune factors into the tumor microenvironment. These mechanisms include immune sensing in the tumor and efficient antigen presentation, as well as important cells and molecules that promote T cell inflamed tumor formation and immune therapy response.
Review
Immunology
Ray A. Ohara, Kenneth M. Murphy
Summary: Cross-priming, initially recognized in cytotoxic T lymphocyte (CTL) responses, involves the presentation of minor histocompatibility antigens by antigen presenting cells (APCs) derived from immunizing cells. As understanding of T cell receptor antigen recognition progressed, cross-priming was redefined as cross-presentation and expanded to include different forms of antigens and APCs not involved in in vivo CTL priming. In vitro cell models have been utilized for studying cross-presentation, but recent studies have shown differences between these models and in vivo APCs. Current research focuses on validating in vivo pathways and gene candidates for cross-presentation, and evaluating their contributions to CTL responses across different antigens and immunologic settings.
SEMINARS IN IMMUNOLOGY
(2023)
Article
Immunology
Frances Sjaastad, Matthew A. Huggins, Erin D. Lucas, Cara Skon-Hegg, Whitney Swanson, Matthew D. Martin, Oscar C. Salgado, Julie Xu, Mark Pierson, Thamotharampillai Dileepan, Tamara A. Kucaba, Sara E. Hamilton, Thomas S. Griffith
Summary: This study suggests that relying solely on specific pathogen-free laboratory mice may limit the effectiveness of vaccine testing, as these mice may have different immune system maturation compared to humans.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Cell Biology
Eva Domenjo-Vila, Valentina Casella, Ryutaro Iwabuchi, Even Fossum, Mireia Pedragosa, Quim Castellvi, Paula Cebollada Rica, Tsuneyasu Kaisho, Kazutaka Terahara, Gennady Bocharov, Jordi Argilaguet, Andreas Meyerhans
Summary: The contribution of XCR1+ dendritic cells (DCs) and SIRPa+ DCs in maintaining T cell function during exhaustion and immunotherapeutic interventions of chronic infections is poorly understood. In a mouse model of chronic LCMV infection, XCR1+ DCs were found to be more resistant to infection and highly activated compared to SIRPa+ DCs. Exploiting XCR1+ DCs through expansion or vaccination improved CD8+ T cell functionality and virus control. PD-L1 blockade was found to be more effective when combined with increased frequency of XCR1+ DCs, enhancing the functionality of exhausted CD8+ T cell subsets.
Article
Allergy
Janice J. Huang, Samantha B. Gaines, Mateo L. Amezcua, Tamar R. Lubell, Peter S. Dayan, Marissa Dale, Alexis D. Boneparth, Mark D. Hicar, Robert Winchester, Mark Gorelik
Summary: This study aimed to analyze the immunologic features of MIS-C patients compared to febrile controls. The results showed that elevated cytokines, activation of type 1 dendritic cells, and dysregulation of NK cells were key features in the pathophysiology of this syndrome.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Lisa G. M. Huis In 't Veld, Nataschja Ho, Melisssa Wassink, Martijn H. den Brok, Gosse J. Adema
Summary: Saponin-based adjuvants (SBAs) are a promising new type of adjuvant that can enhance dendritic cell-mediated antigen cross-presentation and activate CD8 + T cells by inducing lipid bodies. The study reveals that SBAs specifically induce the PERK pathway and depend on its activation for dendritic cell cross-presentation. Additionally, the MHCII(lo)CD11b(hi) subset of dendritic cells shows the highest responsiveness to SBAs.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Dermatology
Anna Brand, Nathalie Diener, Sonja P. Zahner, Christoph Tripp, Ronald A. Backer, Khalad Karram, Aimin Jiang, Ira Mellman, Patrizia Stoitzner, Bjoern E. Clausen
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2020)
Article
Oncology
Lydia Bellmann, Giuseppe Cappellano, Johanna F. Schachtl-Riess, Anastasia Prokopi, Athanasios Seretis, Daniela Ortner, Christoph H. Tripp, Constance E. Brinckerhoff, David W. Mullins, Patrizia Stoitzner
INTERNATIONAL JOURNAL OF CANCER
(2020)
Article
Dermatology
Bjoern E. Clausen, Nikolaus Romani, Patrizia Stoitzner
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2020)
Article
Immunology
Marcel Doerflinger, Yexuan Deng, Paul Whitney, Ranja Salvamoser, Sven Engel, Andrew J. Kueh, Lin Tai, Annabell Bachem, Elise Gressier, Niall D. Geoghegan, Stephen Wilcox, Kelly L. Rogers, Alexandra L. Garnham, Michael A. Dengler, Stefanie M. Bader, Gregor Ebert, Jaclyn S. Pearson, Dominic De Nardo, Nancy Wang, Chenying Yang, Milton Pereira, Clare E. Bryant, Richard A. Strugnell, James E. Vince, Marc Pellegrini, Andreas Strasser, Sammy Bedoui, Marco J. Herold
Article
Dermatology
Lydia Bellmann, Claudia Zelle-Rieser, Paul Milne, Anastasia Resteu, Christoph H. Tripp, Natascha Hermann-Kleiter, Viktoria Zaderer, Doris Wilflingseder, Paul Hoertnagl, Maria Theochari, Jessica Schulze, Mareike Rentzsch, Barbara Del Frari, Matthew Collin, Christoph Rademacher, Nikolaus Romani, Patrizia Stoitzner
Summary: Langerhans cells in the skin, critical for immune response and skin homeostasis, can be generated from monocytes with specific cytokines and ligands. These monocyte-derived LCs show potential for vaccine testing and can be an effective tool for future research.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2021)
Article
Immunology
Dominik Eichin, Alberto Pessia, Akira Takeda, Joni Laakkonen, Lydia Bellmann, Matti Kankainen, Beat A. Imhof, Patrizia Stoitzner, Jing Tang, Marko Salmi, Sirpa Jalkanen
Summary: CD73 plays an unknown role in dampening immune responses on afferent lymphatics, as revealed by this study. Knocking out or suppressing CD73 leads to upregulation of inflammation-associated genes on lymphatic endothelial cells and a more pro-inflammatory phenotype of interacting dendritic cells in vitro and in vivo. Anti-CD73 antibodies have negligible effects on gene expression of lymphatic- and blood-endothelial cells, indicating a low likelihood of endothelial cell-related adverse effects with CD73 targeting therapeutic antibodies.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
M. Isabel G. Lopez Sanchez, Mark Ziemann, Annabell Bachem, Rahul Makam, Jonathan G. Crowston, Carl A. Pinkert, Matthew McKenzie, Sammy Bedoui, Ian A. Trounce
Article
Oncology
Lukas Peiffer, Farnoush Farahpour, Ashwin Sriram, Ivelina Spassova, Daniel Hoffmann, Linda Kubat, Patrizia Stoitzner, Thilo Gambichler, Antje Sucker, Selma Ugurel, Dirk Schadendorf, Jurgen C. Becker
Summary: The study found that treatment with BRAF/MEK inhibitors in melanoma patients allows for an increased expansion of pre-existing melanoma-specific T cells by inducing T-bet and TCF7.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2021)
Article
Oncology
Anastasia Prokopi, Christoph H. Tripp, Bart Tummers, Florian Hornsteiner, Sarah Spoeck, Jeremy Chase Crawford, Derek R. Clements, Mirjana Efremova, Katharina Hutter, Lydia Bellmann, Giuseppe Cappellano, Bruno L. Cadilha, Sebastian Kobold, Louis Boon, Daniela Ortner, Zlatko Trajanoski, Suzie Chen, Tanja D. de Gruijl, Juliana Idoyaga, Douglas R. Green, Patrizia Stoitzner
Summary: Immunotherapy with checkpoint inhibitors has shown impressive results in patients with melanoma, but many still do not benefit from this treatment due to factors such as lack of tumor-infiltrating T cells and loss of intratumoral dendritic cells. In a melanoma mouse model, researchers found that tumor progression is characterized by upregulation of checkpoint molecules and gradual loss of dermal conventional DC 2 subset. Monotherapy with checkpoint blockade was not effective, but boosting DC numbers and activation increased tumor immunogenicity, leading to improved T cell function and delayed tumor growth when combined with antibodies against PD-1 and TIM-3. The study highlights the importance of skin DC in cancer immunotherapy and suggests that restoring DC function is key to enhancing tumor immunogenicity and responsiveness to checkpoint blockade therapy.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Article
Medicine, Research & Experimental
Georgia Deliyannis, Chinn Yi Wong, Hayley A. McQuilten, Annabell Bachem, Michele Clarke, Xiaoxiao Jia, Kylie Horrocks, Weiguang Zeng, Jason Girkin, Nichollas E. Scott, Sarah L. Londrigan, Patrick C. Reading, Nathan W. Bartlett, Katherine Kedzierska, Lorena E. Brown, Francesca Mercuri, Christophe Demaison, David C. Jackson, Brendon Y. Chua
Summary: The study shows that by controlling viral infection in the upper airways, especially through the activation of TLR2 in nasal passages, rapid protection can be provided against severe respiratory infection, especially for at-risk populations. The research emphasizes the importance of nasal epithelial cells and macrophages working together to prevent the spread of respiratory viruses to the vulnerable lower airways.
Article
Dermatology
Christoph H. Tripp, Hermann Voit, Angela An, Magdalena Seidl-Philipp, Johanna Krapf, Stephan Sigl, Nikolaus Romani, Barbara Del Frari, Patrizia Stoitzner
Summary: Dendritic cells are promising targets for immunotherapy of cancer, and laser-assisted epicutaneous immunization may be a valuable alternative to intradermal injection. However, the loading efficacy of DC needs to be further improved.
EXPERIMENTAL DERMATOLOGY
(2021)
Article
Immunology
Katharina Hochheiser, Florian Wiede, Teagan Wagner, David Freestone, Matthias H. Enders, Moshe Olshansky, Brendan Russ, Simone Nussing, Emma Bawden, Asolina Braun, Annabell Bachem, Elise Gressier, Robyn McConville, Simone L. Park, Claerwen M. Jones, Gayle M. Davey, David E. Gyorki, David Tscharke, Ian A. Parish, Stephen Turner, Marco J. Herold, Tony Tiganis, Sammy Bedoui, Thomas Gebhardt
Summary: The deficiency of Ptpn2 affects the formation and function of TRM cells in the skin. The repression of KLRG1 is necessary for optimal TRM cell formation.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Letter
Biochemistry & Molecular Biology
Sven Engel, Marcel Doerflinger, Ariane R. Lee, Andreas Strasser, Marco J. Herold, Sammy Bedoui, Annabell Bachem
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Oncology
Lisa Pipperger, Lydia Riepler, Janine Kimpel, Anita Siller, Patrizia Stoitzner, Zoltan Banki, Dorothee von Laer
Summary: This study examined the interaction of oncolytic viruses with dendritic cells in different species and subsets, showing varied infection, activation, and cytokine production patterns. Matured dendritic cells were less susceptible to VSV-GP infection, while VSV-dM51-GP induced maturation and inflammatory cytokine release. Different dendritic cell types exhibited distinct responses to VSV and VSV-GP variants, highlighting the importance of considering these differences in immunological mechanisms of oncolytic virotherapy.
Article
Dermatology
Klaus Eisendle, Georg Weinlich, Susanne Ebner, Markus Forstner, Daniela Reider, Claudia Zelle-Rieser, Christoph H. Tripp, Peter Fritsch, Patrizia Stoitzner, Nikolaus Romani, Van Anh Nguyen
JOURNAL DER DEUTSCHEN DERMATOLOGISCHEN GESELLSCHAFT
(2020)