Article
Immunology
Cecile Apert, Ariel O. Galindo-Albarran, Sarah Castan, Claire Detraves, Heloise Michaud, Nicola McJannett, Bart Haegeman, Simon Fillatreau, Bernard Malissen, Georg Hollander, Saulius Zuklys, Jeremy C. Santamaria, Olivier P. Joffre, Paola Romagnoli, Joost P. M. van Meerwijk
Summary: This study reevaluated the role of IL-2 and IL-15 in the development of regulatory T-lymphocytes (Treg) and found that IL-2 and IL-15 play important non-redundant quantitative and qualitative roles in intrathymic Treg development.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Carlotta Barahona de Brito, Amiya Kumar Patra
Summary: Transcription factors of the nuclear factor of activated T cell (NFAT) family play a crucial role in T cell function, particularly in the development and maintenance of regulatory T cells. This study found that NFAT factors are critical for the phenotype and survival of regulatory T cells in the thymus and peripheral lymphoid organs, but dispensable for their development.
Article
Immunology
Laura Passeri, Grazia Andolfi, Virginia Bassi, Fabio Russo, Giorgia Giacomini, Cecilia Laudisa, Ilaria Marrocco, Luca Cesana, Marina Di Stefano, Lorella Fanti, Paola Sgaramella, Serena Vitale, Chiara Ziparo, Renata Auricchio, Graziano Barera, Giovanni Di Nardo, Riccardo Troncone, Carmen Gianfrani, Andrea Annoni, Laura Passerini, Silvia Gregori
Summary: Tolerogenic dendritic cells are generated by genetic engineering of monocytes with lentiviral vectors encoding immunodominant antigens and IL-10. These cells downregulate antigen-specific T cell responses and induce antigen-specific regulatory T cells, offering a potential platform for controlling T-cell mediated diseases. In preclinical models, the administration of these tolerogenic dendritic cells prevents the development of type 1 diabetes.
JOURNAL OF AUTOIMMUNITY
(2023)
Article
Biochemistry & Molecular Biology
Sara Fraticelli, Marco Lucioni, Giuseppe Neri, Deborah Marchiori, Caterina Cristinelli, Michele Merli, Rodolfo Monaco, Tiziana Borra, Antonio Lazzaro, Silvia Uccella, Luca Arcaini, Marco Paulli
Summary: Castleman disease (CD) is a rare lymphoproliferative disorder with various clinico-pathological subtypes. The pathogenesis of CD is still uncertain, but alterations in T-cell subsets may contribute to the development of CD. In this study, the distribution of T-cell subsets in the clinico-pathological spectrum of CD was investigated. The results showed decreased CD4/CD8 ratio and lower number of FOXP3+ T-reg cells in CD cases compared to non-specific reactive lymph nodes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Mohammad Afzal Khan, Ghazi Abdulmalik Ashoor, Talal Shamma, Fatimah Alanazi, Abdullah Altuhami, Shadab Kazmi, Hala Abdalrahman Ahmed, Abdullah Mohammed Assiri, Dieter Clemens Broering
Summary: Interleukin-10 plays a crucial role in maintaining immunotolerance and promoting graft microvascular repair and fibrosis suppression. IL-10 depletion exacerbates airway epithelial injury and fibrosis, while IL-10 reconstitution facilitates airway epithelial repair and fibrosis suppression.
Article
Medicine, Research & Experimental
Lisa Y. Beppu, Raja Gopal Reddy Mooli, Xiaoyao Qu, Giovanni J. Marrero, Christopher A. Finley, Allen N. Fooks, Zachary P. Mullen, Adolfo B. Frias, Ian Sipula, Bingxian Xie, Katherine E. Helfrich, Simon C. Watkins, Amanda C. Poholek, Sadeesh K. Ramakrishnan, Michael J. Jurczak, Louise M. D'Cruz
Summary: IL-10, produced by CD4(+)Foxp3(+) regulatory T cells, can suppress adipocyte beiging, leading to reduced insulin resistance and obesity. Loss of IL-10 in Tregs, through the transcription factor Blimp-1, resulted in improved metabolic outcomes in high-fat diet-fed mice. This highlights the important role of Treg-derived IL-10 in regulating adipose tissue homeostasis.
Article
Biochemistry & Molecular Biology
Laura Perucho, Laura Icardi, Elisabetta Di Simone, Veronica Basso, Alessandra Agresti, Amaia Vilas Zornoza, Teresa Lozano, Felipe Prosper, Juan Jose Lasarte, Anna Mondino
Summary: Sin3A plays a crucial role in regulating the differentiation of Th17 cells and the development of lymphocytes.
Article
Biology
Dong Hyun Kim, Hee Young Kim, Sunjung Cho, Su-Jin Yoo, Won-Ju Kim, Hye Ran Yeon, Kyungho Choi, Je-Min Choi, Seong Wook Kang, Won-Woo Lee
Summary: The study demonstrates the crucial role of IL-1RII receptor in regulating IL-17 responses, affecting the functionality of CD4(+) T cells by limiting IL-1 beta responsiveness.
Article
Multidisciplinary Sciences
Akimichi Inaba, Zewen Kelvin Tuong, Tian X. X. Zhao, Andrew P. P. Stewart, Rebeccah Mathews, Lucy Truman, Rouchelle Sriranjan, Jane Kennet, Kourosh Saeb-Parsy, Linda Wicker, Frank Waldron-Lynch, Joseph Cheriyan, John A. A. Todd, Ziad Mallat, Menna R. R. Clatworthy
Summary: Activation of B cells expressing CD25 can be enhanced by low-dose recombinant IL-2, leading to increased secretion of IL-10. Administration of low dose IL-2 to patients results in an increase in IL-10-producing B cells. Single-cell RNA sequencing reveals enrichment of regulatory B cell gene signature in plasma cell populations from IL-2-treated patients, with significant down-regulation of the transcriptional repressor BACH2. BACH2 suppression leads to increased B cell IL-10, suggesting its role in promoting an immunoregulatory phenotype in B cells through IL-2 modulation.
NATURE COMMUNICATIONS
(2023)
Article
Allergy
Sofia Tagkareli, Maria Salagianni, Ioanna-Evdokia Galani, Maria Manioudaki, Eleftherios Pavlos, Kalliopi Thanopoulou, Evangelos Andreakos
Summary: The study identified a novel regulatory T-cell population expressing CD103, which is programmed to prevent excessive type 2 inflammation and maintain homeostasis in the respiratory tract. This has important therapeutic implications for allergic and autoimmune disorders.
Article
Oncology
Wan-Chung Hu
Summary: The study utilized IL-10 and IL-15 to activate the TH alpha beta-mediated immune response for treating breast cancer and neuroblastoma, showing a reduction in tumor volume and increase in survival. This approach proves to be a promising strategy for cancer treatment.
FRONTIERS IN ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Melba Munoz, Ahmed N. Hegazy, Tobias M. Brunner, Vivien Holecska, Roman M. Marek, Anja Froehlich, Max Loehning
Summary: Th2 cells lacking T-bet exhibit strong immunomodulatory potential by suppressing antigen-specific CD8(+) T cell responses via IL-10. These cells protect mice against virus-induced type 1 diabetes and hinder effector and cytotoxic CD8(+) T cell development by downregulating dendritic cell activation. IL-10-secreting Th2 cells could potentially be used as a therapeutic approach for T cell-mediated inflammatory disorders.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Oncology
Christine Weissenborn, Sophie von Lenthe, Nicole Hinz, Stefanie Langwisch, Mandy Busse, Anne Schumacher, Ana C. Zenclussen, Stefan Fest
Summary: This study found that transient ablation of Tregs hindered the growth of neuroblastoma (NB), while the absence of Bregs had no effect on NB growth, providing important evidence for targeting Tregs as a potential therapy for this aggressive childhood tumor.
INTERNATIONAL JOURNAL OF CANCER
(2022)
Article
Dermatology
Keisuke Kobayashi, Sakiko Chikazawa, Yue Chen, So Suzuki, Nao Ichimasu, Kazumoto Katagiri
Summary: Sex hormones, particularly estrogen, have dual effects on the pathogenesis of psoriasis. Estrogen can suppress inflammation by enhancing IL-10 production, but can also enhance inflammation by inducing IL-22 and IL-23 expression.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Marta Fortunato, Giada Amodio, Silvia Gregori
Summary: Tolerogenic dendritic cells (tolDC) play a central role in immune homeostasis and peripheral tolerance. We developed a protocol to generate genetically engineered tolDC overexpressing IL-10 (DCIL-10) and found that they can modulate cytotoxic CD8(+) T cell responses by reducing proliferation and activation, and inducing anergic CD8(+) T cells without signs of exhaustion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Andrea Annoni, Silvia Gregori, Luigi Naldini, Alessio Cantore
CELLULAR IMMUNOLOGY
(2019)
Review
Immunology
Michela Comi, Giada Amodio, Silvia Gregori
FRONTIERS IN IMMUNOLOGY
(2018)
Article
Immunology
Anke Fuchs, Mateusz Gliwinski, Nathali Grageda, Rachel Spiering, Abul K. Abbas, Silke Appel, Rosa Bacchetta, Manuela Battaglia, David Berglund, Bruce Blazar, Jeffrey A. Bluestone, Martin Bornhaeuser, Anja ten Brinke, Todd M. Brusko, Nathalie Cools, Maria Cristina Cuturi, Edward Geissler, Nick Giannoukakis, Karolina Golab, David A. Hafler, S. Marieke van Ham, Joanna Hester, Keli Hippen, Mauro Di Ianni, Natasa Ilic, John Isaacs, Fadi Issa, Dorota Iwaszkiewicz-Grzes, Elmar Jaeckel, Irma Joosten, David Klatzmann, Hans Koenen, Cees van Kooten, Olle Korsgren, Karsten Kretschmer, Megan Levings, Natalia Maria Marek-Trzonkowska, Marc Martinez-Llordella, Djordje Miljkovic, Kingston H. G. Mills, Joana P. Miranda, Ciriaco A. Piccirillo, Amy L. Putnam, Thomas Ritter, Maria Grazia Roncarolo, Shimon Sakaguchi, Silvia Sanchez-Ramon, Birgit Sawitzki, Ljiljana Sofronic-Milosavljevic, Megan Sykes, Qizhi Tang, Marta Vives-Pi, Herman Waldmann, Piotr Witkowski, Kathryn J. Wood, Silvia Gregori, Catharien M. U. Hilkens, Giovanna Lombardi, Phillip Lord, Eva M. Martinez-Caceres, Piotr Trzonkowski
FRONTIERS IN IMMUNOLOGY
(2018)
Article
Immunology
Laurence Pellerin, Ping Chen, Silvia Gregori, Gabriela Hernandez-Hoyos, Rosa Bacchetta, Maria Grazia Roncarolo
FRONTIERS IN IMMUNOLOGY
(2018)
Article
Multidisciplinary Sciences
Fabrizia Bonacina, David Coe, Guosu Wang, Maria P. Longhi, Andrea Baragetti, Annalisa Moregola, Katia Garlaschelli, Patrizia Uboldi, Fabio Pellegatta, Liliana Grigore, Lorenzo Da Dalt, Andrea Annoni, Silvia Gregori, Qingzhong Xiao, Donatella Caruso, Nico Mitro, Alberico L. Catapano, Federica M. Marelli-Berg, Giuseppe D. Norata
NATURE COMMUNICATIONS
(2018)
Article
Oncology
Giada Amodio, Joanna Cichy, Patricia Conde, Gianluca Matteoli, Aurelie Moreau, Jordi Ochando, Barbaros H. Oral, Michaela Pekarova, Elizabeth J. Ryan, Johannes Roth, Yahya Sohrabi, Maria-Cristina Cuturi, Silvia Gregori
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2019)
Review
Immunology
Maria Grazia Roncarolo, Silvia Gregori, Rosa Bacchetta, Manuela Battaglia, Nicola Gagliani
Article
Cell Biology
Marco Andreani, Silvia Gregori
Article
Immunology
Michela Comi, Daniele Avancini, Francesca Santoni de Sio, Matteo Villa, Molly Javier Uyeda, Matteo Floris, Daniela Tomasoni, Alessandro Bulfone, Maria Grazia Roncarolo, Silvia Gregori
CELLULAR & MOLECULAR IMMUNOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Giorgia Squeri, Laura Passerini, Francesca Ferro, Cecilia Laudisa, Daniela Tomasoni, Federica Deodato, Maria Alice Donati, Serena Gasperini, Alessandro Aiuti, Maria Ester Bernardo, Bernhard Gentner, Luigi Naldini, Andrea Annoni, Alessandra Biffi, Silvia Gregori
Article
Medicine, Research & Experimental
Fabio Russo, Eliana Ruggiero, Rosalia Curto, Laura Passeri, Francesca Sanvito, Ileana Bortolomai, Anna Villa, Silvia Gregori, Andrea Annoni
Summary: The study developed a gene transfer approach to modify the T cell repertoire in thymic epithelial cells, correcting the autoimmune response in type 1 diabetes. The strategy involved intrathymic injection of a lentiviral vector to achieve stable transgene expression in thymic epithelial cells, leading to successful correction of the disease in mice models.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2022)
Article
Andrology
Andrea Salonia, Marina Pontillo, Paolo Capogrosso, Edoardo Pozzi, Anna Maria Ferrara, Alice Cotelessa, Federico Belladelli, Christian Corsini, Silvia Gregori, Isaline Rowe, Cristina Carenzi, Giuseppe A. Ramirez, Cristina Tresoldi, Massimo Locatelli, Giulio Cavalli, Lorenzo Dagna, Antonella Castagna, Alberto Zangrillo, Moreno Tresoldi, Giovanni Landoni, Patrizia Rovere-Querini, Fabio Ciceri, Francesco Montorsi
Summary: Total testosterone levels gradually increase in men after recovery from COVID-19. However, nearly 30% of men still have low testosterone levels suggestive of hypogonadism during a minimum 12-month follow-up period.
Article
Biochemistry & Molecular Biology
Marta Fortunato, Giada Amodio, Silvia Gregori
Summary: Tolerogenic dendritic cells (tolDC) play a central role in immune homeostasis and peripheral tolerance. We developed a protocol to generate genetically engineered tolDC overexpressing IL-10 (DCIL-10) and found that they can modulate cytotoxic CD8(+) T cell responses by reducing proliferation and activation, and inducing anergic CD8(+) T cells without signs of exhaustion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Daniele Avancini, Alessandro Testori, Lucia Fresolone, Grazia Andolfi, Michela Vuono, Vittorio Martinelli, Francesca R. Santoni de Sio, Silvia Gregori
Summary: IL-10 induces the generation of tolerogenic dendritic cells (tolDC) from monocytes through the activation of aryl hydrocarbon receptor (AHR). AHR activity downstream of IL-10 signaling is required for the induction of tolerogenic activities in DCs, and this IL-10/AHR genomic signature is active in circulating DCs in healthy individuals. However, in multiple sclerosis patients, an altered IL-10/AHR signature is observed, which correlates with functional defects and reduced frequencies of IL-10-induced tolDCs in vitro and in vivo.
Article
Biophysics
R. Crocchiolo, O. Ringden, J-O Bay, D. Blaise, B. Omasic, B. Mazzi, C. Picard, S. Trinca, L. Barkholt, J. Peccatori, S. Gregori, G. Amodio, K. Fleischhauer, F. Ciceri, M. Bregni
BONE MARROW TRANSPLANTATION
(2018)
