Review
Biotechnology & Applied Microbiology
Adolf Acheampong, Lamei Li, Shereen M. Elsherbiny, Yahui Wu, Mohammed Sharif Swallah, Precious Bondzie-Quaye, Qing Huang
Summary: This review investigates strategies to enhance Astaxanthin production from H. pluvialis, focusing on genetic and environmental factors and transcriptomic dynamics. Genetic engineering and culture optimization are effective approaches to increase Astaxanthin production, while new technologies may become principal strategies in the future.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Agricultural Engineering
Baobei Wang, Xueshan Pan, Fang Wang, Lulu Liu, Jing Jia
Summary: This study analyzed the biochemical compositions and proteomics of mixotrophic Haematococcus pluvialis under different light conditions. The findings showed that high light exposure increased astaxanthin, carbohydrate, and fatty acid contents, and regulated multiple metabolic pathways. A regulatory model for mixotrophic Haematococcus pluvialis coping with high light stress was proposed.
BIORESOURCE TECHNOLOGY
(2022)
Review
Agricultural Engineering
Yuanyua Ren, Jinquan Deng, Junchao Huang, Zhaoming Wu, Lanbo Yi, Yuge Bi, Feng Chen
Summary: Astaxanthin, an important antioxidant and nutraceutical compound, is widely used in dietary supplements and cosmetics. The refinery of astaxanthin from Haematococcus pluvialis is a growing industrial sector, as it can accumulate astaxanthin up to 5% of dry weight. Further research on production optimization and joint-production with lipids could make algal-derived astaxanthin more accessible for various applications.
BIORESOURCE TECHNOLOGY
(2021)
Review
Agricultural Engineering
Byung Sun Yu, So Young Lee, Sang Jun Sim
Summary: Industrialization has led to excessive CO2 emissions, causing unnatural warming and triggering a need for biological conversion of CO2. While H. pluvialis has high market value, challenges such as contamination risks in large-scale cultivation due to slow growth rate need to be addressed.
BIORESOURCE TECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Petras Venckus, Ieva Endriukaityte, Kotryna Cekuolyte, Renata Gudiukaite, Andrius Pakalniskis, Egle Lastauskiene
Summary: This study tested the effects of silver nanoparticles on Haematococcus pluvialis and found that high concentrations of AgNPs significantly reduced culture productivity in green-stage cultures, while the impact on red-stage cultures was smaller. The addition of AgNPs also led to changes in cell morphology, including deformations, disrupted cytokinesis, and induced sexual reproduction.
Article
Agricultural Engineering
Chunli Yu, Hui-Ping Wang, Tengsheng Qiao, Yongteng Zhao, Xuya Yu
Summary: The study found that intermittent succinic acid feeding method in fed-batch culture significantly promoted the production of astaxanthin and lipids in Haematococcus pluvialis, resulting in the highest productivity levels.
BIORESOURCE TECHNOLOGY
(2021)
Article
Green & Sustainable Science & Technology
Gannoru Kankanamalage Sanuji Hasara Nishshanka, Vinoj Chamilka Liyanaarachchi, Pemaththu Hewa Viraj Nimarshana, Thilini Udayangani Ariyadasa, Jo-Shu Chang
Summary: The microalga Haematococcus pluvialis has high biotechnological potential for producing various valuable compounds. Biorefining, which integrates multiple production processes in a single facility, can maximize the utilization of feedstock and reduce resource consumption. This review discusses the potential of biorefining H. pluvialis to promote more sustainable and cleaner production technologies.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Agricultural Engineering
Yongteng Zhao, Hui-Ping Wang, Chunli Yu, Wei Ding, Benyong Han, Shuxiang Geng, Delu Ning, Ting Ma, Xuya Yu
Summary: The study revealed that melatonin treatment upregulated the biosynthesis of astaxanthin and lipids in Haematococcus pluvialis, affecting the accumulation process through the regulation of gene expression and metabolic pathways. The treatment may promote coproduction of astaxanthin and lipids by regulating the carbon-nitrogen balance and antioxidant system.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Jae Been Lee, Yun Hwan Park, Min Seo Jeon, Sok Kim, Yoon-E Choi
Summary: In this study, the researchers successfully increased the production of astaxanthin in Haematococcus pluvialis by combining polyethylenimine (PEI) with chitosan. The polyethylenimine concentration had an effect on astaxanthin accumulation, while the molecular weight of chitosan did not. The treatment also increased the reactive oxygen species content in cells, leading to the upregulation of enzymes involved in astaxanthin biosynthesis. The PEI-chitosan fiber treatment showed high recyclability and maintained over 90% astaxanthin production efficiency.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Agricultural Engineering
Hang Guo, Tao Li, Yongteng Zhao, Xuya Yu
Summary: Copper treatment significantly increased astaxanthin and lipid synthesis in Haematococcus pluvialis, leading to upregulation of biosynthesis genes and antioxidant enzyme-related genes, as well as a decrease in reactive oxygen species levels. Additionally, copper induced the activation of protein kinases, facilitating the production of astaxanthin and lipids in the algae.
BIORESOURCE TECHNOLOGY
(2021)
Article
Agricultural Engineering
Vaibhav Sunil Tambat, Anil Kumar Patel, Reeta Rani Singhania, Akash Pralhad Vadrale, Archana Tiwari, Chiu-Wen Chen, Cheng-Di Dong
Summary: This study focused on designing and optimizing a two-stage mixotrophic bioprocess using Chlorella zofingiensis to produce astaxanthin. Gradual increase in light intensity and micronutrient concentration during the first stage and stress conditions during the second stage were found to enhance biomass and astaxanthin yield. The study also showed the potential of using Chlorella zofingiensis for commercial production of astaxanthin and lipid, with promising sustainability features.
BIORESOURCE TECHNOLOGY
(2023)
Article
Chemistry, Applied
Walter Pitacco, Chiara Samori, Laura Pezzolesi, Virginia Gori, Antonio Grillo, Matteo Tiecco, Martina Vagnoni, Paola Galletti
Summary: Three novel hydrophobic deep eutectic solvents (DESs) based on oleic acid and terpenes were prepared and used to extract astaxanthin. The DESs showed high efficiency in extracting astaxanthin and their chemical composition was safe and edible. The carotenoid profile obtained using DESs was similar to that obtained using traditional organic solvents. The thymol:oleic acid DES (TAO) exhibited superior antioxidant properties and could preserve astaxanthin content after light exposure, making it a promising formulation for the food ingredients/additives industry.
Review
Agricultural Engineering
Bolam Kim, Soo Youn Lee, Aditya Lakshmi Narasimhan, Sangui Kim, You-Kwan Oh
Summary: The green microalga Haematococcus pluvialis is a valuable source of astaxanthin, a powerful antioxidant widely used in various industries. The unique cyst-like cell with a rigid three-layered cell wall formed during astaxanthin accumulation presents challenges for extraction. Various cell disruption methods and downstream processes for sustainable development are also discussed in this review.
BIORESOURCE TECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Kebi Wu, Kezhen Ying, Jin Zhou, Dai Liu, Lu Liu, Yi Tao, James Hanotu, Xiaoshan Zhu, Zhonghua Cai
Summary: This study aimed to address the issue of limited carbon supply affecting the growth rate of Haematococcus pluvialis in mass production, by designing a novel microbubble-driven photobioreactor that improved CO2 transfer efficiency and cell growth rate.
Article
Chemistry, Multidisciplinary
Hana-Nur Fitriana, Soo-Youn Lee, Sun-A Choi, Ji-Ye Lee, Bo-Lam Kim, Jin-Suk Lee, You-Kwan Oh
Summary: This study investigated the technical feasibility of inducing astaxanthin biosynthesis in H. pluvialis using electric stimulation in a two-chamber bioelectrochemical system. An optimal voltage of 4 V (anodic current: 30 mA) effectively stimulated astaxanthin biosynthesis, resulting in a 36.9% increase in astaxanthin content in treated cells compared to untreated cells. Proper regulation of bioelectrochemical parameters can improve astaxanthin production in H. pluvialis culture using electric treatment.
APPLIED SCIENCES-BASEL
(2021)
Article
Energy & Fuels
Yao Wang, Yi-Ying Lee, Tonya M. Santaus, Charles E. Newcomb, Jin Liu, Chris D. Geddes, Chengwu Zhang, Qiang Hu, Yantao Li
BIOENERGY RESEARCH
(2017)
Review
Biotechnology & Applied Microbiology
Sangram K. Lenka, Nicole Carbonaro, Rudolph Park, Stephen M. Miller, Ian Thorpe, Yantao Li
BIOTECHNOLOGY ADVANCES
(2016)
Article
Biotechnology & Applied Microbiology
Jin Liu, Yi-Ying Lee, Xuemei Mao, Yantao Li
JOURNAL OF APPLIED PHYCOLOGY
(2017)
Article
Plant Sciences
Jin Liu, Danxiang Han, Kangsup Yoon, Qiang Hu, Yantao Li
Article
Biochemistry & Molecular Biology
Yi Xin, Yandu Lu, Yi-Ying Lee, Li Wei, Jing Jia, Qintao Wang, Dongmei Wang, Fali Bai, Hanhua Hu, Qiang Hu, Jin Liu, Yantao Li, Jian Xu
Article
Plant Sciences
Yu Zhang, Ying Ye, Wei Ding, Xuemei Mao, Yantao Li, Henri Gerken, Jin Liu
Article
Plant Sciences
Yanhai Gong, Nam K. Kang, Young U. Kim, Zengbin Wang, Li Wei, Yi Xin, Chen Shen, Qintao Wang, Wuxin You, Jong-Min Lim, Suk-Won Jeong, Youn-Il Park, Hee-Mock Oh, Kehou Pan, Eric Poliner, Guanpin Yang, Yonghua Li-Beisson, Yantao Li, Qiang Hu, Ansgar Poetsch, Eva M. Farre, Yong K. Chang, Won-Joong Jeong, Byeong-ryool Jeong, Jian Xu
Article
Engineering, Environmental
Zixuan Wang, Yi-Ying Lee, David Scherr, Ryan S. Senger, Yantao Li, Zhen He
Review
Biochemical Research Methods
Hanzhi Lin, Yantao Li, Russell T. Hill
Summary: This article reviews the recent advances in using phytohormones to promote microalgal growth and discusses the biosynthesis of phytohormones in microalgal-bacterial associations and their implications for algal physiology and metabolite production.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Plant Sciences
Juan Yang, Jin Liu, Yufang Pan, Eric Marechal, Alberto Amato, Meijing Liu, Yangmin Gong, Yantao Li, Hanhua Hu
Summary: The oleaginous alga Nannochloropsis oceanica stores specific phosphatidyldiacylethanolamines for triacylglycerol production under stress. The NoPDAT pathway is parallel and independent of the NoDGAT pathway for oil production in N. oceanica. LU-PE can serve as an alternative carbon sink for photosynthetically assimilated carbon in N. oceanica when PDAT-mediated TAG biosynthesis is compromised or under stress in the presence of high CO2 levels.
Review
Environmental Sciences
Pengfei Cheng, Yantao Li, Chun Wang, Jiameng Guo, Chengxu Zhou, Renchuan Zhang, Yiwei Ma, Xiaochen Ma, Lu Wang, Yanling Cheng, Xiaojun Yan, Roger Ruan
Summary: Marine microalgae have the potential to be used as feedstock for biofuels and other valuable compounds, but currently it is not economically viable. Developing efficient cultivation systems and production modes, as well as screening dominant algal strains, are necessary for utilizing algal biomass for biorefinery applications.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Plant Sciences
Yi-Ying Lee, Rudolph Park, Stephen M. Miller, Yantao Li
Summary: Genetic compensation is proposed as a mechanism to explain phenotypic differences between gene knockouts and knockdowns. This study demonstrates examples of genetic compensation in microalgae and suggests its physiological relevance in TAG biosynthesis under stress. These findings provide guidance for future genetic engineering and mutant characterization efforts.
Editorial Material
Plant Sciences
Yantao Li
JOURNAL OF PHYCOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Hehong Wei, Ying Shi, Xiaonian Ma, Yufang Pan, Hanhua Hu, Yantao Li, Ming Luo, Henri Gerken, Jin Liu
BIOTECHNOLOGY FOR BIOFUELS
(2017)
Article
Shailendra Kumar Singh, Samuel R. Major, Haiyuan Cai, Feng Chen, Russell T. Hill, Yantao Li
GENOME ANNOUNCEMENTS
(2018)