Article
Biochemistry & Molecular Biology
Chih-Hsien Wu, Hsuan-Hwai Lin, Yi-Ying Wu, Yi-Lin Chiu, Li-Yen Huang, Cheng-Chung Cheng, Chung-Chi Yang, Tsung-Neng Tsai
Summary: Coronary artery disease is a major global issue. Patients with coronary artery disease often develop peptic ulcers when receiving aspirin treatment, making panendoscopy with biopsy necessary for diagnosis and treatment. A protocol for generating iPSCs from gastric fibroblasts via biopsy was established, with successful differentiation into functional cardiomyocytes demonstrated. This study represents the first in vitro generation of iPSCs from gastric fibroblasts.
Article
Medicine, Research & Experimental
Yu Gao, Liping Su, Yuhua Wei, Shihua Tan, Zhenyu Hu, Zhonghao Tao, Jean-Paul Kovalik, Tuck Wah Soong, Jianyi Zhang, Jun Pu, Lei Ye
Summary: Ascorbic acid (AA) treatment increases MLC2v protein expression and promotes the formation of ventricular-like cardiomyocytes (CMs) in hiPSC-CMs. However, the effect of AA is attenuated with inhibition of TET1/TET2-mediated DNA demethylation. This study provides new insights into the maturation and application of hiPSC-CMs.
Article
Cell Biology
Jingsi Yang, Nan Ding, Dandan Zhao, Yunsheng Yu, Chunlai Shao, Xuan Ni, Zhen-Ao Zhao, Zhen Li, Jianquan Chen, Zheng Ying, Miao Yu, Wei Lei, Shijun Hu
Summary: The study developed a new strategy of using EBSS treatment to mimic in vivo starvation event, promoting the maturation of hESC-CMs. Intermittent starvation through EBSS significantly enhanced structural, metabolic, and electrophysiological maturity of hESC-CMs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Liang Ye, Xinyuan Zhang, Qin Zhou, Bin Tan, Hao Xu, Qin Yi, Liang Yan, Min Xie, Yin Zhang, Jie Tian, Jing Zhu
Summary: Activation of AMPK improves the maturation of hiPSC-CMs by increasing the expression of CM-specific markers, improving myocardial structure, enhancing mitochondrial function, and promoting mitochondrial fusion.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell & Tissue Engineering
Jiangtao Li, Xin Feng, Xiang Wei
Summary: This review summarizes the methods of establishing cardiomyocyte models using induced pluripotent stem cells (iPSCs) and the progress in genome editing techniques. Because the currently cultured iPSC-CMs are immature, researchers have attempted various methods to promote their maturation. Many researchers have established iPSC-CM models of hypertrophic cardiomyopathy (HCM) and used diverse methods for measurements.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Sijia Ji, Wanzhi Tu, Chenwen Huang, Ziyang Chen, Xinyue Ren, Bingqing He, Xiaoyan Ding, Yuelei Chen, Xin Xie
Summary: This study reveals that the Aurora kinase inhibitor CYC116 significantly promotes the maturation of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs). It increases the expression of genes related to cardiomyocyte function, improves the organization of sarcomeres, increases the number of mitochondria, and enhances the physiological function of the cells.
MOLECULES AND CELLS
(2022)
Review
Biotechnology & Applied Microbiology
Yang Xiao, Yihuan Chen, Chunlai Shao, Yaning Wang, Shijun Hu, Wei Lei
Summary: Myocardial infarction is a common cardiovascular disease caused by ischemia leading to cardiomyocyte damage and scar tissue formation. Current research focuses on improving the transplantation efficacy of PSC-CMs to enhance the treatment of myocardial infarction.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell Biology
Maria Faleeva, Ivan Diakonov, Prashant Srivastava, Masoud Ramuz, Gaia Calamera, Kjetil Wessel Andressen, Nadja Bork, Lorenza Tsansizi, Marie-Victoire Cosson, Andreia Sofia Bernardo, Viacheslav Nikolaev, Julia Gorelik
Summary: This study investigates the development and signal compartmentation of the cGMP pathway during the differentiation of human pluripotent stem cell-derived cardiomyocytes. The results show that different cGMP pathways mature at different stages and are regulated by different phosphodiesterases. Furthermore, caveolae are found to play a crucial role in the maturation of the beta(3)-adrenoreceptor-cGMP pathway in prolonged cultures of hPSC-CMs.
Article
Biochemistry & Molecular Biology
Marzia De Bortoli, Viviana Meraviglia, Katarina Mackova, Laura S. Frommelt, Eva Konig, Johannes Rainer, Chiara Volani, Patrizia Benzoni, Maja Schlittler, Giada Cattelan, Benedetta M. Motta, Claudia Volpato, Werner Rauhe, Andrea Barbuti, Serena Zacchigna, Peter P. Pramstaller, Alessandra Rossini
Summary: This study used human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) to establish a model for incomplete penetrance in arrhythmogenic cardiomyopathy (ACM). The results showed molecular and functional differences between ACM and asymptomatic carriers of the same mutation (ASY) hiPSC-CMs, including a higher amount of mutated PKP2 mRNA, a lower expression of the connexin-43 protein, a lower overall density of sodium current, a higher intracellular lipid accumulation, and sarcomere disorganization in ACM compared to ASY hiPSC-CMs. Differentially expressed genes were also found, supporting a predisposition for a fatty phenotype in ACM hiPSC-CMs. This indicates that hiPSC-CMs are a suitable model to study incomplete penetrance in ACM.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Cell & Tissue Engineering
Nino Chirico, Elise L. Kessler, Renee G. C. Maas, Juntao Fang, Jiabin Qin, Inge Dokter, Mark Daniels, Tomo Saric, Klaus Neef, Jan-Willem Buikema, Zhiyong Lei, Pieter A. Doevendans, Joost P. G. Sluijter, Alain van Mil
Summary: In this study, a fast, easy, and economical method was developed to induce maturation of iPSC-CMs via PPAR/PGC-1 alpha activation using small molecule activators AA and GW. Treatment with AA or GW led to increased metabolic, structural, functional, and electrophysiological maturation of iPSC-CMs.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Cell Biology
M. Juliana Gomez-Garcia, Elya Quesnel, Rasha Al-attar, Andrew R. Laskary, Michael A. Laflamme
Summary: This review discusses the immature phenotype of human pluripotent stem cell derived cardiomyocytes (hPSC-CMs) and potential strategies for their maturation, considering the developmental processes and signaling cues involved in normal human cardiac maturation. Various approaches for hPSC-CM maturation, such as prolonged culture duration, metabolic manipulation, and tissue engineering, are summarized, along with evidence of maturation post-implantation in injured hearts and its implications for potential hPSC-based cardiac therapy.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Yuki Tanaka, Shin Kadota, Jian Zhao, Hideki Kobayashi, Satomi Okano, Masaki Izumi, Yusuke Honda, Hajime Ichimura, Naoko Shiba, Takeshi Uemura, Yuko Wada, Shinichiro Chuma, Tsutomu Nakada, Shugo Tohyama, Keiichi Fukuda, Mitsuhiko Yamada, Tatsuichiro Seto, Koichiro Kuwahara, Yuji Shiba
Summary: Long-term cultured mature hiPSC-CMs promote engraftment, maturation, and angiogenesis post-transplantation in infarcted rat hearts, enhancing the therapeutic potential of hiPSC-CMs.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cell & Tissue Engineering
Gabriel Peinkofer, Martina Maass, Kurt Pfannkuche, Agapios Sachinidis, Stephan Baldus, Juergen Hescheler, Tomo Saric, Marcel Halbach
Summary: The developmental stage at the time of transplantation plays a crucial role in the persistence and functional integration of transplanted iPSC-CM, with cells at day 14 of differentiation showing the highest persistence and adhesive capability in vivo.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cardiac & Cardiovascular Systems
Thomas Eschenhagen, Katrin Ridders, Florian Weinberger
Summary: Heart regeneration is essential for treating heart disease by remuscularizing lost myocardium. While there has been progress in the past decade, there are still many important questions to be answered in order to optimize this approach for regenerative therapy of heart failure.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2022)
Review
Cardiac & Cardiovascular Systems
Sophia E. Silver, Ryan W. Barrs, Ying Mei
Summary: Cardiovascular disease is a leading cause of global mortality, and alternative cardiac regenerative therapies, such as hPSC-derived cardiomyocytes, show promising therapeutic potential for heart repair. Despite challenges and limitations, solutions are being explored to address these issues towards successful clinical translation and future perspectives on hPSC-CM transplantation.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Engineering, Biomedical
Taiyun Chi, Jong Seok Park, Jessica C. Butts, Tracy A. Hookway, Amy Su, Chengjie Zhu, Mark P. Styczynski, Todd C. McDevitt, Hua Wang
IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS
(2015)
Article
Biochemical Research Methods
Tracy A. Hookway, Jessica C. Butts, Emily Lee, Hengli Tang, Todd C. McDevitt
Article
Multidisciplinary Sciences
Jessica C. Butts, Dylan A. McCreedy, Jorge Alexis Martinez-Vargas, Frederico N. Mendoza-Camacho, Tracy A. Hookway, Casey A. Gifford, Praveen Taneja, Linda Noble-Haeusslein, Todd C. McDevitt
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Multidisciplinary Sciences
Andrew S. Khalil, Xiaohua Yu, Angela W. Xie, Gianluca Fontana, Jennifer M. Umhoefer, Hunter J. Johnson, Tracy A. Hookway, Todd C. McDevitt, William L. Murphy
SCIENTIFIC REPORTS
(2017)
Article
Cell & Tissue Engineering
Hannah A. Strobel, Tracy A. Hookway, Marco Piola, Gianfranco Beniamino Fiore, Monica Soncini, Eben Alsberg, Marsha W. Rolle
TISSUE ENGINEERING PART A
(2018)
Editorial Material
Biotechnology & Applied Microbiology
Tracy A. Hookway
TRENDS IN BIOTECHNOLOGY
(2018)
Article
Multidisciplinary Sciences
Hannah A. Strobel, Elizabeth L. Calamari, Brittany Alphonse, Tracy A. Hookway, Marsha W. Rolle
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2018)
Article
Cell & Tissue Engineering
Tracy A. Hookway, Oriane B. Matthys, Federico N. Mendoza-Camacho, Sarah Rains, Jessica E. Sepulveda, David A. Joy, Todd C. McDevitt
TISSUE ENGINEERING PART A
(2019)
Article
Cell & Tissue Engineering
Diwakar Turaga, Oriane B. Matthys, Tracy A. Hookway, David A. Joy, Meredith Calvert, Todd C. McDevitt
TISSUE ENGINEERING PART C-METHODS
(2020)
Article
Cell & Tissue Engineering
Oriane B. Matthys, Tracy A. Hookway, Todd C. McDevitt
CURRENT STEM CELL REPORTS
(2016)
Proceedings Paper
Engineering, Electrical & Electronic
Muneeb Zia, Taiyun Chi, Chaoqi Zhang, Paragkumar Thadesar, Tracy Hookway, Joe Gonzalez, Todd McDevitt, Hua Wang, Muhannad S. Bakir
2015 IEEE INTERNATIONAL ELECTRON DEVICES MEETING (IEDM)
(2015)
Meeting Abstract
Cell & Tissue Engineering
T. Hookway, N. Votaw, T. McDevitt
TISSUE ENGINEERING PART A
(2015)
Proceedings Paper
Engineering, Electrical & Electronic
Jong Seok Park, Taiyun Chi, Jessica Butts, Tracy Hookway, Todd McDevitt, Hua Wang
2015 IEEE INTERNATIONAL SOLID-STATE CIRCUITS CONFERENCE DIGEST OF TECHNICAL PAPERS (ISSCC)
(2015)
Proceedings Paper
Computer Science, Information Systems
Hua Wang, Alborz Mahdavi, Jongseok Park, Taiyun Chi, Jessica Butts, Tracy A. Hookway, Todd McDevitt, David A. Tirrell, Ali Hajimiri
2014 IEEE BIOMEDICAL CIRCUITS AND SYSTEMS CONFERENCE (BIOCAS)
(2014)
Proceedings Paper
Engineering, Biomedical
Tracy A. Hookway, Doan Nguyen, Chunhui Xu, Mary B. Wagner, Todd C. McDevitt
2014 40TH ANNUAL NORTHEAST BIOENGINEERING CONFERENCE (NEBEC)
(2014)