期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 464, 期 1, 页码 83-88出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2015.05.114
关键词
DNA methylation; Nilaparvata lugens; Gene expression; RNA interference; Female fecundity
资金
- National Basic Research Program of China [2012CB114102]
DNA methylation is an ancient epigenetic modification present in all three domains of life. However, the understanding of DNA methylation in insects is limited. Here, we amplified the full-length transcripts of the DNA methyltransferases Nlu-Dnmt1 and Nlu-Dnmt3, indicating that a complete DNA methylation toolkit exists in the brown planthopper, Nilaparvata lugens, a destructive pest in rice production. Nlu-DNMT1 and Nlu-DNMT3 had the conserved motifs and domains of the DNA methyltransferase family. Nlu-Dnmt1 and Nlu-Dnmt3 were highly expressed in the mated and gravid female adults but weakly expressed in larvae, male adults, and virgin female adults. Silencing Nlu-Dnmt1 and Nlu-Dnmt3 in gravid brachypterous female adults led to fewer offspring, suggesting that DNA methylation regulates female fecundity in insects. (C) 2015 Elsevier Inc. All rights reserved.
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