Article
Environmental Sciences
Duantao Cao, Shuhong He, Xin Li, Lihong Shi, Feiyan Wang, Sumei Yu, Shiji Xu, Chao Ju, Hua Fang, Yunlong Yu
Summary: The bacterial strain Arthrobacter sp. C2 was isolated for atrazine degradation, showing degradation rates of 0.34, 1.94, and 18.64 mg/L/d at different concentrations. The optimal conditions for degradation were at 30°C and pH 7.0-9.0, and a metabolic pathway for atrazine degradation was proposed. The introduction of strain C2 into soil can enhance atrazine degradation and eliminate its toxic effects on wheat growth.
Article
Agricultural Engineering
Abhishek Singh, Anand Kumar Pandey, Suresh Kumar Dubey
Summary: The study showed that Arthrobacter sp. BHU FT2 strain has a high potential for efficient degradation of isoprene, which could be utilized in large-scale bio-filtration units for isoprene degradation.
BIORESOURCE TECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Xin Zhang, Rongrong Zhu, Weilin Li, Junwei Ma, Hui Lin
Summary: This study investigated the antibiotic resistance pattern of a bacterium with substantial biodegradation capabilities, finding that it exhibited resistance to at least seven categories of antibiotics. The presence of oxytetracycline increased the tolerance of the bacterium to streptomycin sulphate. This research contributes to a deeper understanding of the behavioral characteristics of resistance genes and the adaptive evolution of drug-resistant bacteria.
SCIENTIFIC REPORTS
(2021)
Article
Environmental Sciences
Xiaoxu Wang, Belay Tafa Oba, Hui Wang, Qing Luo, Jiaxin Liu, Lanxin Tang, Miao Yang, Hao Wu, Lina Sun
Summary: A novel bacterium, Arthrobacter globiformis DC-1, capable of degrading DDT as its sole carbon and energy source, was isolated from DDT-contaminated agricultural soil. The bacterium can efficiently grow and degrade DDT at a wide range of concentrations, with the optimal degradation rate observed at 10 mg/LDDT with peptone as the carbon source at pH 7.5 and 30°C. This strain can also simultaneously degrade DDE, DDD, and DDT, with degradation rates reaching over 60% in 10 days. The biodegradation pathway of DDT by A. globiformis DC-1 involves dechlorination, dehydrochlorination, and mineralization to carbon dioxide.
Article
Environmental Sciences
Zhao Jiang, Shijie Deng, Lu Wang, Yang Hu, Bo Cao, Jun Lv, Jianhua Qu, Lei Wang, Yifan Wang, Ying Zhang
Summary: The study found that nicosulfuron inhibits the removal efficiency of atrazine, induces the over-production of ROS, resulting in damaged cell membranes, decreased cell viability, and reduced trzN transcription.
ENVIRONMENTAL POLLUTION
(2021)
Article
Environmental Sciences
Peng Wang, Tingchen Cui, Jialu Li, Na Liu, Mei Hong
Summary: The bacterium Arthrobacter sp. WN18, grown in Tetrahydrofuran (THF), showed efficient degradation of 1,4-dioxane (dioxane) with a THF to dioxane ratio of 3:1. It exhibited high tolerance to THF at concentrations up to 7.0 g/L and potential for dioxane removal both in pure culture and microcosms. Microcosm assays and bioaugmentation experiments confirmed WN18's ability to degrade dioxane effectively, indicating its potential for future field applications.
WATER AIR AND SOIL POLLUTION
(2021)
Article
Chemistry, Physical
Jiameng Zhang, Zhiliang Yu, Yaling Gao, Meini Wang, Kai Wang, Tao Pan
Summary: A newly isolated cadmium-resistant bacterial strain from herbicides-polluted soil in China has the ability to use atrazine as the sole carbon, nitrogen, and energy source for growth. This strain shows high degradation capability for atrazine and high resistance to cadmium.
Article
Engineering, Environmental
Xuejun Wang, Hao Wu, Xiaoyu Wang, Haixia Wang, Kankan Zhao, Bin Ma, Zhenmei Lu
Summary: DBP contamination decreases microbial community diversity and weakens potential interactions between microorganisms. DBP shifts the interactions between strain ZM05 and other microbes, and nondegrading bacterium Pseudomonas aeruginosa ZM03 has a positive interaction with strain ZM05 during DBP degradation under pH stress.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Agricultural Engineering
Ispreet Kaur, Vivek Kumar Gaur, Saloni Rishi, Vandana Anand, Shashank Kumar Mishra, Rajeev Gaur, Anju Patel, Suchi Srivastava, Praveen C. Verma, Pankaj Kumar Srivastava
Summary: In this study, the biodegradation potential of 9 Ascomycetes fungi isolated from a hexachlorocyclohexane dumpsite soil for lindane was tested. The strain Pleurostoma richardsiae (FN5) showed a lindane biodegradation rate constant (K value) of 0.144 d-1 and a half-life of 4.8 days. The study also revealed the formation of intermediate metabolites and the potential pathway involved in lindane biodegradation by the novel fungal strain FN5.
BIORESOURCE TECHNOLOGY
(2023)
Article
Environmental Sciences
Haiping Gu, Jie Yan, Yuhao Liu, Xuewei Yu, Yan Feng, Xuanyi Yang, Su Shiung Lam, Mu. Naushad, Cheng Li, Christian Sonne
Summary: Bioaugmentation was used to obtain a microbiome capable of degrading polycyclic aromatic hydrocarbons (PAHs). The study found that acclimating microorganisms to soil supplemented with phenanthrene led to the enrichment of PAH-degrading bacteria, including Actinobacteriota and genera such as Streptomyces, Rhodococcus, Nocardioides, Sphingomonas, and Mycobacterium. The degradation efficiency of phenanthrene in aqueous and soil cultures inoculated with phenanthrene-acclimated soil was found to be high. This research provides theoretical and practical support for the bioremediation of PAHs in the environment.
ENVIRONMENTAL RESEARCH
(2023)
Review
Chemistry, Analytical
Yizheng Li, Qinghui Liu, Muhammad Junaid, Guanglong Chen, Jun Wang
Summary: In recent years, there has been growing concern over the prevalence and accumulation of microplastics (MPs) in the soil environment due to their resistance to degradation and long-term ecological impacts. However, there is still a lack of critical reviews on the ecological effects and effective remediation methods of MPs in soil compared to aquatic ecosystems. This review summarizes the distribution characteristics, sources, transportation, and identification techniques of MPs in soil, as well as the interactions between MPs contamination and soil ecosystems. It also discusses the effects and degradation mechanisms of different organisms on MPs degradation, highlighting the potential breakthrough of transforming enzyme stability and performance through computer protein design. This review provides guidance for further exploration of feasible approaches and advanced remediation strategies for MPs contamination in the future.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Environmental Sciences
Fang Wang, Longfeng Wei, Meiting Guo, Wansheng Yang, Zhu Li, Deyu Hu, Ping Lu
Summary: This study investigated the effect of microorganisms on the degradation of BIT residues in different soil conditions using 16S rRNA gene sequencing. The results showed significant changes in the structure and abundance of bacterial communities in BIT soils, while there was no significant difference in the fungal community. Certain bacterial phyla played a crucial role in the degradation of BIT, and soil organic matter and pH had significant effects on the microbial community.
Review
Environmental Sciences
Junyu Zhu, Guowen Dong, Fu Feng, Jing Ye, Ching-Hua Liao, Chih-Hung Wu, Sheng-Chung Chen
Summary: This article focuses on the main sources of MPs in soil and highlights that MP generation is not solely a byproduct of plastic production, but can also result from the impact of biological and abiotic factors during the transport process. The article presents a new perspective on the degradation of MPs in soil, suggesting that the main transformation mediated by abiotic factors occurs on the soil surface, while the main biodegradation occurs in the soil interior.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Rabia Najme, Shulin Zhuang, Jiguo Qiu, Zhenmei Lu
Summary: The bacterium Arthrobacter sp. strain NOR5 was found to completely degrade nornicotine under optimal conditions, forming a novel metabolite 2,6DHPONor. This study provides important information for understanding nornicotine metabolism.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Engineering, Environmental
Kankana Kundu, Aileen Melsbach, Benjamin Heckel, Sarah Schneidemann, Dheeraj Kanapathi, Sviatlana Marozava, Juliane Merl-Pham, Martin Elsner
Summary: This study investigates the adaptation of bacteria to low concentrations of pollutants by studying the degradation of 4-chlorophenol (4-CP) by Arthrobacter chlorophenolicus A6. The researchers found that at lower concentrations, there was an increase in isotope fractionation of 4-CP, indicating a change in enzyme activity. Surprisingly, high concentrations were associated with increased cell wall permeability. These findings suggest that bacteria adapt to low concentrations through regulation of enzyme activity and provide insights into bacterial adaptation mechanisms at low concentrations.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Environmental Sciences
Sandra Mosquera, Ioannis Stergiopoulos, Johan H. J. Leveau
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2020)
Article
Microbiology
Hung K. Doan, Maria L. Antequera-Gomez, Atul N. Parikh, Johan H. J. Leveau
FRONTIERS IN MICROBIOLOGY
(2020)
Review
Biotechnology & Applied Microbiology
Tyler S. Laird, Neptali Flores, Johan H. J. Leveau
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Fidele N. Akum, Ravi Kumar, Gary Lai, Catherine H. Williams, Hung K. Doan, Johan H. J. Leveau
Summary: A bacterial mixture of Collimonas arenae Cal35 and Bacillus velezensis FZB42 showed synergistic effects in protecting tomato plants from a soilborne fungal pathogen, Fusarium oxysporum f.sp. lycopersici, while the individual strains were less effective. Mutants with impaired ability to halt fungal growth highlighted the importance of specific gene clusters in Cal35 for producing an antifungal compound called carenaemins, which contributed significantly to the observed synergy with FZB42. The study suggests the potential for enhancing the efficacy of Bacillus-based biocontrol products by supplementing them with Collimonas bacteria.
MICROBIAL BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Sandra Mosquera, Johan H. J. Leveau, Ioannis Stergiopoulos
Summary: Our study reveals that prolonged exposure to Collimonas fungivorans selects for fungal lineages with reduced germinability and slower germination, even in the absence of the bacterium. Conversely, absence of the bacteria leads to fungal lineages with increased germinability and faster germination. Responses of fungi to biotic factors related to antagonism may be predictable and reversible to some extent.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Maria Luisa Antequera-Gomez, Luis Diaz-Martinez, Juan Antonio Guadix, Ana Maria Sanchez-Tevar, Sara Sopena-Torres, Jesus Hierrezuelo, Hung K. Doan, Johan H. J. Leveau, Antonio deVicente, Diego Romero
Summary: Bacillus cereus is a common food-borne pathogen that can cause food poisoning in humans, with symptoms of vomiting or diarrhea. The study found that sporulation is necessary for the survival of B. cereus in leaves, but not essential in ready-to-eat vegetables. It also highlighted the underestimated number of food poisoning cases associated with B. cereus and the need to revise detection protocols based on spores and toxins.
MICROBIAL BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ryan Kawakita, Johan H. J. Leveau, Tina Jeoh
Summary: Research has shown that adjusting the temperature, spray rate, and alginate concentration during the spray-drying encapsulation process of alginate microcapsules (CLAMs) can improve the yield and survival rate of the product. Additionally, using modified starch in the CLAMs encapsulation can prolong the shelf survival of the bacteria to 3 weeks.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Muhammad Arshad, Tahir Naqqash, Muhammad Tahir, Johan H. Leveau, Ahmad Zaheer, Syeda Anjum Tahira, Nasir Ahmad Saeed, Shaheen Asad, Muhammad Sajid
Summary: This study compared the bacterial populations, root exudates, and soil enzyme activities in nontransgenic and AVP1-transgenic wheat rhizosphere. The results showed that the AVP1 gene in transgenic wheat had no apparent adverse effects on the soil environment and bacterial communities.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Review
Microbiology
Gillian E. Bergmann, Johan H. J. Leveau
Summary: Microorganisms can influence the germination and fitness of plant seeds, thereby affecting plant health and community dynamics. However, the variability of seed-associated microbiota across individuals, plant species, and environments makes it challenging to identify the dominant processes underlying the assembly, composition, and influence of these communities. This study proposes that metacommunity ecology provides a useful framework for studying the microbiota of developing seeds, incorporating principles of filtering, species interactions, and dispersal at different scales.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Engineering, Chemical
Jiyoon Yi, Johan H. J. Leveau, Nitin Nitin
Summary: Conventional washing and sanitizing methods have limited effectiveness in killing bacteria on leafy greens. This study uses numerical simulation and experimental validation to show the impact of leaf surface topography and chlorine delivery systems on the inactivation of leaf-attached bacteria.
JOURNAL OF FOOD ENGINEERING
(2022)
Article
Plant Sciences
Peter M. Henry, Samuel I. Koehler, Sukhwinder Kaur, Lynn Epstein, Jeffrey P. Mitchell, Thomas R. Gordon, Johan H. J. Leveau
Summary: This study developed a high-throughput translation elongation factor 1 alpha (EF-1 alpha) amplicon-sequencing method for the fungal genus Fusarium. The analysis revealed that nonpathogenic strains of F. oxysporum did not uniformly colonize crops in the root communities. Changes in bulk soil Fusarium communities were minimal after one year of crop rotation and conservation tillage, suggesting that climate and soil physicochemical properties have a greater impact on Fusarium community structure.
PHYTOBIOMES JOURNAL
(2022)
Article
Microbiology
Stephane Uroz, Oceane Geisler, Laure Fauchery, Raphael Lami, Alice M. S. Rodrigues, Emmanuelle Morin, Johan H. J. Leveau, Philippe Oger
Summary: Collimonas bacteria are well-adapted to nutrient-poor environments and have various abilities such as chitin hydrolysis, antifungal metabolite production, mineral weathering, and biocontrol of plant fungal diseases. This study investigated the role of N-acyl homoserine lactones (AHLs) in Collimonas ecology through transcriptomic analyses and functional assays. The results showed that AHLs play a role in environment colonization, metabolism readjustment, and microbial interactions, as well as identified genes and metabolic pathways related to AHL production and regulation.
FEMS MICROBIOLOGY ECOLOGY
(2022)
Article
Microbiology
Nadav Kashtan, Benjamin Bushong, Johan H. J. Leveau
Summary: A key challenge in microbiome science is the scale mismatch problem, where the scale of sampling and averaging microbial communities is different from the scale of interactions between individual microorganisms and their environment. This perspective explores how economists deal with the scale mismatch problem and how their approaches could benefit the field of microbiomics.
Article
Plant Sciences
Hung K. Doan, Viviane N. Ngassam, Sean F. Gilmore, Robin Tecon, Atul N. Parikh, Johan H. J. Leveau
PHYTOBIOMES JOURNAL
(2020)
Article
Plant Sciences
Hung K. Doan, Nilesh N. Maharaj, Kaitlyn N. Kelly, Eugene M. Miyao, R. Michael Davis, Johan H. J. Leveau
PHYTOBIOMES JOURNAL
(2020)