Article
Immunology
Yanting Xu, Ziyi Xu, Xiaobin Gu, Yue Xie, Ran He, Jing Xu, Bo Jing, Xuerong Peng, Guangyou Yang
Summary: In this study, two arginine kinases (AKs) in Sarcoptes scabiei were found to play important roles in the host immune response. These AKs promote cell proliferation and inhibit cell apoptosis by activating the NF-κB signaling pathway and increasing the expression of Bcl-2 and Bcl-xl. Additionally, they also alter the immune balance by affecting the secretion of specific cytokines, participating in the allergic inflammation caused by S. scabiei.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Kristian Espeland, Andrius Kleinauskas, Petras Juzenas, Andreas Brech, Sagar Darvekar, Vlada Vasovic, Trond Warloe, Eidi Christensen, Jorgen Jahnsen, Qian Peng
Summary: This study investigates the effects of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) on cytokines and exosomes of human healthy peripheral blood mononuclear cells (PBMCs). The results show that ALA-PDT treatment can reduce the levels of cytokines and exosomes, indicating potential anti-inflammatory and immunomodulatory effects.
Article
Immunology
Wang Jianfang, Wang Hui, Kang Le
Summary: In this study, the expression and effect of LINC00870 in Mycobacterium tuberculosis (MTB) infection were investigated. The results showed that LINC00870 was significantly increased in MTB-infected peripheral blood mononuclear cells (PBMCs) and its overexpression inhibited Th1-secreted cytokines while promoted Th2-secreted cytokines. Furthermore, LINC00870 promoted the activation of JAK/STAT signaling in MTB-infected PBMCs. Higher levels of LINC00870 were also found in sputum and plasma samples from TB and LTBI patients. After three months of therapy, the levels of LINC00870 decreased in both TB and LTBI patients. Therefore, LINC00870 may be a novel biomarker for diagnosing and treating tuberculosis.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Cell Biology
Stefania Croci, Martina Bonacini, Giovanni Dolci, Marco Massari, Nicola Facciolongo, Elisa Pignatti, Alessandra Pisciotta, Gianluca Carnevale, Aurelio Negro, Giulia Cassone, Francesco Muratore, Lucia Belloni, Alessandro Zerbini, Carlo Salvarani
Summary: Mesenchymal stem cells from human dental pulp can modulate the production of cytokines in COVID-19 patients, suggesting a potential therapeutic use in COVID-19.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mona Amani, Elham Shokati, Kobra Entezami, Samaneh Khorrami, Mir Hadi Jazayeri, Elahe Safari
Summary: This study evaluated the immunomodulatory effects of Low Molecular Weight Garlic Proteins (LMWGP) on the proliferation of immune cells, induction of regulatory T cells, and cytokine secretion. The results showed that LMWGP increased PBMCs proliferation, decreased Tregs induction, and modulated cytokine secretion, suggesting its potential as a beneficial immunomodulatory supplement in cancer herbal medicine.
PROCESS BIOCHEMISTRY
(2021)
Article
Pharmacology & Pharmacy
Vera Vorstandlechner, Dragan Copic, Katharina Klas, Martin Direder, Bahar Golabi, Christine Radtke, Hendrik J. Ankersmit, Michael Mildner
Summary: This study demonstrates the anti-fibrotic effect of PBMCsec on cutaneous scars in both mouse and human experimental settings. PBMCsec regulates the expression of fibrosis-related genes, attenuates myofibroblast differentiation, and inhibits the breakdown of elastic fibers. These findings suggest that PBMCsec could be a novel therapeutic option for treating skin scarring.
Article
Materials Science, Multidisciplinary
Byeongyeon Kim, In-Ho Seo, Gwang Myeong Seo, Woo-Joong Kim, Eui-Cheol Shin, Sungyoung Choi
Summary: The study introduces a blood divider (B-DV) as a centrifuge tube insert to prevent RBC contamination during PBMC harvest, simplifying the separation process and enabling rapid, easy PBMC isolation.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Biochemistry & Molecular Biology
Kristian Espeland, Andrius Kleinauskas, Petras Juzenas, Sagar Darvekar, Vlada Vasovic, Trond Warloe, Eidi Christensen, Jorgen Jahnsen, Qian Peng
Summary: Photodynamic therapy (PDT) using 5-aminolevulinic acid (ALA) has been shown to induce apoptosis and necrosis in target lesions. This study investigated the effects of ALA-PDT on peripheral blood mononuclear cell (PBMC) subsets from patients with active Crohn's disease (CD). ALA-PDT killed monocytes and downregulated levels of cytokines and exosomes associated with inflammation, suggesting its potential as a treatment for CD and other immune-mediated diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Veterinary Sciences
Seo-Young Hwang, Ju-Hyun An, Kyung-Bo Kim, Jeong-Hwa Lee, Su-Min Park, Ye-In Oh, Hyung Kyu Chae, Hwa-Young Youn
Summary: This study evaluated the role of PBMCs in obesity-related chronic inflammation and found that the gene expression levels of inflammation-related adipokines and pro-inflammatory cytokines were altered in PBMCs, potentially contributing to the low-grade chronic inflammation observed in obesity.
VETERINARY MEDICINE AND SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Belay Tessema, Janine Riemer, Ulrich Sack, Brigitte Koenig
Summary: The results of CSA parameters measured in PBMCs are influenced by the PBMC isolation method, age, seasonal variation, and gender. Researchers and physicians should properly interpret the results of CSA parameters in PBMCs by considering these factors. Separate CSA evaluation standards should be used based on the isolation method, age, gender, and season-dependent factors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Lechuang Chen, Rui Chen, Min Yao, Zhimin Feng, Guoxiang Yuan, Fengchun Ye, Kien Nguyen, Jonathan Karn, Grace A. McComsey, Thomas M. McIntyre, Ge Jin
Summary: Elevated cytokine production in COVID-19 patients is associated with disease progression and severity. This study found that plasma exosomes of COVID-19 patients contain SARS-CoV-2 dsRNA, which can stimulate immune cells to produce inflammatory cytokines. These exosomes protect the dsRNA cargo and deliver it to recipient cells, inducing proinflammatory responses. Additionally, the study identified divergent signaling pathways in different immune cells in response to COVID-19 plasma exosomes.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Faruque Parvez, Fredine T. Lauer, Pam Factor-Litvak, Tariqul Islam, Mahbubul Eunus, M. Abu Horayara, Mizanour Rahman, Golam Sarwar, Habibul Ahsan, Joseph H. Graziano, Scott W. Burchiel
Summary: Limited evidence suggests that environmental exposure to arsenic affects the immune system in adults. Urinary arsenic and its metabolites have been found to modulate the number of T-helper 17 cells and IL-17A cytokine production. The associations between urinary arsenic and Th17 cell numbers are nonlinear in non-smoking women, while significant decreases in Th17 cell numbers are observed in smoking males. Vitamin D levels can modify these associations.
Article
Medicine, General & Internal
Keiichiro Yoshioka, Hironori Sato, Takeshi Kawasaki, Daisuke Ishii, Takuro Imamoto, Mitsuhiro Abe, Yoshinori Hasegawa, Osamu Ohara, Koichiro Tatsumi, Takuji Suzuki
Summary: RNA sequencing analysis revealed differential gene expression patterns in peripheral blood mononuclear cells (PBMCs) between patients with pulmonary sarcoidosis and healthy controls, with 270 differentially expressed genes involved in various biological processes related to the pathobiology of sarcoidosis. Enrichment analysis of gene ontology and KEGG pathways indicated the potential involvement of specific signaling pathways in sarcoidosis pathogenesis, providing insight into potential therapeutic targets for the disease.
FRONTIERS IN MEDICINE
(2022)
Article
Multidisciplinary Sciences
Maria D. I. Manunta, Giuseppe Lamorte, Francesca Ferrari, Elena Trombetta, Mario Tirone, Cristiana Bianco, Alessandra Cattaneo, Luigi Santoro, Guido Baselli, Manuela Brasca, Mahnoosh Ostadreza, Elisa Erba, Andrea Gori, Alessandra Bandera, Laura Porretti, Luca V. C. Valenti, Daniele Prati
Summary: The infection of SARS-CoV-2 virus causes COVID-19, which leads to changes in blood cell composition, particularly an abnormally high number of neutrophils in early cases, potentially affecting the severity of the disease.
SCIENTIFIC REPORTS
(2021)
Article
Agriculture, Dairy & Animal Science
Michaela Bunting, Bethan Challice, Amanda Gibson, Steven van Winden
Summary: This study investigated the association between copper levels and monocyte plasticity, showing that increased copper levels reduced cell viability and affected the types of monocytes in cows of different ages. CD14 expression influenced CD16 expression and nitric oxide production.
