Review
Biochemistry & Molecular Biology
Min Heui Yoo, A-Ram Lee, Kyoung-Sik Moon
Summary: Cell therapy products have limitations such as storage instability and toxicity issues. Extracellular vesicles (EVs) secreted from cells show potential for therapeutic agent development. EVs contain various materials and avoid safety concerns related to tumorigenicity. However, non-clinical studies and safety evaluations of EVs are challenging.
Article
Biochemical Research Methods
Virginie Stevenin, Jacques Neefjes
Summary: The links between bacterial infections and cancer are actively being investigated. This study presents a cost-effective soft agar colony formation assay to quantify cell transformation after Salmonella Typhimurium infection. The protocol includes details on infection, seeding cells in soft agar, and automated cell colony enumeration. This assay can be adapted for studying other bacteria or host cells.
Review
Biochemistry & Molecular Biology
Nefeli Lagopati, Konstantinos Belogiannis, Andriani Angelopoulou, Angelos Papaspyropoulos, Vassilis Gorgoulis
Summary: P14ARF is a well-characterized tumor suppressor involved in regulating cell cycle arrest and apoptosis in response to oncogenic stimuli. It is frequently lost through methylation in a variety of human malignancies and has been linked to melanomas and glioblastomas through mutations and deletions. In addition to its role in tumorigenesis, P14ARF is also involved in developmental processes such as spermatogenesis, mammary gland development, ocular development, and angiogenesis.
Article
Oncology
Hiu Yeung Lau, Jingyi Tang, Patrick J. Casey, Mei Wang
Summary: The anchorage-independent soft-agar colony formation assay has been widely used as a bridge between adherent cell cultures and animal tumor studies to predict the tumorigenicity of cancer cells. However, the extraction of biological macromolecules from cells embedded in soft-agar matrices has been a challenge. New methods have been developed to extract DNA, RNA, and proteins directly from cells grown in soft agar, enabling molecular signaling analysis.
Article
Cell Biology
Xiangnan Zhang, Shuo Liang, Enze Wang, Ning Tao
Summary: The study aimed to build cellular aggregates of TS/A and normal fibroblasts or CAFs, and verify the value of this model in drug screening and the effect of CD44 on aggregate formation. The results showed that cellular aggregates were successfully formed in the improved culture medium, CAFs promoted the formation of cancer cell aggregates, eugenol had value in drug screening, and CD44 had an important effect on aggregate formation.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Review
Multidisciplinary Sciences
Abdulmajeed F. Alrefaei
Summary: Frizzled receptors play crucial roles in development and disease, with their dysfunction leading to various diseases and potential therapeutic targets.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Lubna Khreesha, Abdallah Barjas Qaswal, Baheth Al Omari, Moath Ahmad Albliwi, Omar Ababneh, Ahmad Albanna, Abdelrahman Abunab'ah, Mohammad Iswaid, Salameh Alarood, Hasan Guzu, Ghadeer Alshawabkeh, Fuad Mohammed Zayed, Mohammad Awad Abuhilaleh, Mohammad Nayel Al-Jbour, Salameh Obeidat, Aiman Suleiman
Summary: This study presents a quantum tunneling model to explain the depolarizing action of lithium ions through closed gates of voltage-gated sodium channels, showing that this model provides a more comprehensive explanation of lithium-induced membrane depolarization compared to the classical model. Additionally, the quantum model distinguishes between the two lithium isotopes (Li-6 and Li-7) in terms of depolarization and highlights the high quantum membrane conductance achieved by lithium ions at the resting state.
