期刊
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
卷 -, 期 73, 页码 -出版社
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/50466
关键词
Medicine; Issue 73; Molecular Biology; Biomedical Engineering; Immunology; Ophthalmology; Surgery; Calcium Metabolism Disorders; Glucose Metabolism Disorders; Diabetes Mellitus; Hyperglycemia; Hyperinsulinism; Hypoglycemia; Transplantation; pancreatic islets; islet; intraocular; anterior chamber; eye; cornea; living window; in vivo imaging; immune responses; cannula; imaging; animal model
资金
- Diabetes Research Institute Foundation
- NIH/NIDDK/NIAID [F32DK083226, NIH RO3DK075487, U01DK089538]
- Karolinska Institutet
- Swedish Research Council
- Swedish Diabetes Foundation
- Family Erling-Persson Foundation
- Family Knut and Alice Wallenberg Foundation
- Skandia Insurance Company Ltd.
- VIBRANT [FP7-228933-2]
- Strategic Research Program in Diabetes at Karolinska Institutet
- Novo Nordisk Foundation
- Berth von Kantzow's Foundation
- Novo Nordisk Fonden [NNF12OC1016557] Funding Source: researchfish
Intravital imaging has emerged as an indispensable tool in biological research. In the process, many imaging techniques have been developed to study different biological processes in animals non-invasively. However, a major technical limitation in existing intravital imaging modalities is the inability to combine non-invasive, longitudinal imaging with single-cell resolution capabilities. We show here how transplantation into the anterior chamber of the eye circumvents such significant limitation offering a versatile experimental platform that enables non-invasive, longitudinal imaging with cellular resolution in vivo. We demonstrate the transplantation procedure in the mouse and provide representative results using a model with clinical relevance, namely pancreatic islet transplantation. In addition to enabling direct visualization in a variety of tissues transplanted into the anterior chamber of the eye, this approach provides a platform to screen drugs by performing long-term follow up and monitoring in target tissues. Because of its versatility, tissue/cell transplantation into the anterior chamber of the eye not only benefits transplantation therapies, it extends to other in vivo applications to study physiological and pathophysiological processes such as signal transduction and cancer or autoimmune disease development.
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