Article
Biochemistry & Molecular Biology
M. Paul Murphy, Valeria A. Buzinova, Carrie E. Johnson
Summary: Progress has been made in the treatment of Alzheimer's disease through the development of anti-A beta therapeutics, which have shown modest efficacy in slowing the progression of the disease. However, the puzzling issue remains as to why completely removing A beta does not fully stop the disease.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Review
Biochemistry & Molecular Biology
Liisa Lutter, Liam D. Aubrey, Wei-Feng Xue
Summary: Predicting the highly ordered three-dimensional structures of amyloid protein fibrils from their monomeric self-assembly precursors remains a challenging aspect of the classical protein folding problem, due to the polymorphic nature of amyloid assembly. Understanding the diversity and individuality of amyloid structures, as well as the link to biology and disease phenotypes, is essential. Current research focuses on resolving the structural basis and biological consequences of polymorphic amyloid assemblies using various techniques such as cryo-EM, ssNMR, and AFM.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Review
Medicine, General & Internal
Thamonwan Diteepeng, Federica del Monte, Marco Luciani
Summary: This review discusses the recent progress in understanding protein misfolding and compromised protein quality control in cardiovascular diseases, highlighting the lack of successful translation from preclinical models to clinical settings. More collaboration between basic scientists and clinicians is needed to develop specific biomarkers and therapeutic strategies to target proteotoxicity in CVD patients.
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Biotechnology & Applied Microbiology
Edward Chau, Jin Ryoun Kim
Summary: In this study, a new molecular probe, PG65-MIMO, was developed and shown to provide comprehensive information on the aggregation states of alpha-synuclein (alpha S). It fills the technological gap in alpha S detection and offers a promising tool for studying Parkinson's Disease.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biology
Pascal Dominic Rem, Vita Sereikaite, Diego Fernandez-Fernandez, Sebastian Reinartz, Daniel Ulrich, Thorsten Fritzius, Luca Trovo, Salome Roux, Ziyang Chen, Philippe Rondard, Jean-Philippe Pin, Jochen Schwenk, Bernd Fakler, Martin Gassmann, Tania Rinaldi Barkat, Kristian Stromgaard, Bernhard Bettler
Summary: Amyloid-beta precursor protein (APP) regulates neuronal activity through the release of secreted APP (sAPP) acting at cell surface receptors. A 17 amino acid peptide (APP17) derived from APP binds to the extracellular sushi domain 1 (SD1) of GABA(B) receptors (GBRs). However, APP17 does not influence GBR activity in heterologous cells, indicating that sAPP exerts its neuronal effects through receptors other than GBRs.
Article
Neurosciences
Johan N. K. Larsson, Sofie Nystroem, Per Hammarstrom
Summary: Neurodegenerative diseases are associated with the accumulation of misfolded proteins. HSP10, a molecular chaperone, plays an important role in protein homeostasis in these diseases by influencing fibril formation and structure.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Edward Chau, Hyunjoo Kim, Jineun Shin, Alberto Martinez, Jin Ryoun Kim
Summary: The study found that AM17 can not only inhibit the aggregation of alpha S monomers, but also disaggregate alpha S oligomers and fibrils, independent of copper ions. Resveratrol also showed similar inhibitory effects on alpha S aggregation only in the presence of copper ions.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Jin-Beom Si, Bokyung Kim, Jin Hae Kim
Summary: TTR is a crucial transporter of thyroid hormone and retinol binding protein in human plasma and cerebrospinal fluid, yet it is also known for its amyloidogenic nature leading to various amyloidoses. Research has shown that decreased stability of TTR's native tetrameric conformation is the main cause of these diseases, and recent multidisciplinary investigations have shed light on the mechanistic details of TTR amyloidogenic transformation. Special emphasis has been placed on identifying novel structural features in amyloidogenic species of TTR and discussing the proteolysis-induced fragmentation mechanism that promotes TTR amyloidosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Mueed Ur Rahman, Ashfaq Ur Rehman, Taaha Arshad, Hai-Feng Chen
Summary: The study reveals the disaggregation mechanism of Amyloid using molecular tweezer CLR01, showing that CLR01 can effectively disaggregate amyloid by interacting with Lysine and stretching out the N-terminal chain. Furthermore, CLR01 remodels the pentamer Prion into a compact structure, which may resist further oligomerization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Multidisciplinary
Oxana V. Galzitskaya, Olga M. Selivanova, Elena Y. Gorbunova, Leila G. Mustaeva, Viacheslav N. Azev, Alexey K. Surin
Summary: Under certain conditions, proteins and peptides can self-assemble into various supramolecular structures with potential pathological or functional implications. Fibrils, films, and amyloid gels are promising objects for nanobiotechnology research. Understanding the mechanism of structure formation and its influencing factors is crucial for developing nanobiomaterials with desired properties.
Article
Biochemistry & Molecular Biology
Kartikay Sharma, Sambhasan Banerjee, Dilan Savran, Cedric Rajes, Sebastian Wiese, Amandeep Girdhar, Nadine Schwierz, Christopher Lee, James Shorter, Matthias Schmidt, Lin Guo, Marcus Faendrich
Summary: In this study, the three-dimensional structure of amyloid fibrils from the hnRNPA1 protein was investigated using cryo-electron microscopy. The fibril core was found to be composed of a 45-residue segment from the low-complexity domain of the protein, while the remaining parts formed a fuzzy coat around the core. The fibril exhibited a pseudo-21 screw symmetry and contained disease-associated mutation sites. These findings suggest that the structure of full-length protein amyloid fibrils may be more complex than currently believed.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Joel N. Buxbaum, Angela Dispenzieri, David S. Eisenberg, Marcus Faendrich, Giampaolo Merlini, Maria J. M. Saraiva, Yoshiki Sekijima, Per Westermark
Summary: The Nomenclature Committee of the International Society of Amyloidosis has proposed a nomenclature recommendation, adding six new proteins to the list of human amyloid fibril proteins, including intracellular amyloid fibrils associated with neurodegenerative diseases.
AMYLOID-JOURNAL OF PROTEIN FOLDING DISORDERS
(2022)
Article
Biochemistry & Molecular Biology
Kamile Mikalauskaite, Mantas Ziaunys, Vytautas Smirnovas
Summary: This study examines the impact of the initial folding state of a protein on amyloid fibril formation, revealing a correlation between protein state and the kinetics and structural properties of the resulting fibrils.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Brett K-Y Chu, Ruei-Fong Tsai, Chien-Lun Hung, Yun-Hsuan Kuo, Eric H. -L Chen, Yun-Wei Chiang, Sunney Chan, Rita P. -Y Chen
Summary: This study used a combination of site-directed nitroxide spin-labeling, fibril seeding, and electron spin resonance (ESR) spectroscopy to determine the structure of in vitro-prepared full-length mouse prion fibrils. The formation of the amyloid core was found to be linked to an alpha-to-beta structural transformation involving the segment 160-224. This method is suitable for examining the structure of hetero-seeded amyloid fibrils.
Article
Biochemistry & Molecular Biology
Surbhi Chaudhary, Asmita Dhiman, Anil Patidar, Himanshu Malhotra, Sharmila Talukdar, Rahul Dilawari, Gaurav Kumar Chaubey, Radheshyam Modanwal, Chaaya Iyengar Raje, Manoj Raje
Summary: Studies have shown that the cytoplasmic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) can prevent protein aggregation, maintain proteins in a soluble state, and interact with cyPrP and httQ-103.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Virology
William E. Matchett, Stephanie S. Anguiano-Zarate, Pramod N. Nehete, Kathryn Shelton, Bharti P. Nehete, Guojun Yang, Stephanie Dorta-Estremera, Philip Barnette, Peng Xiao, Siddappa N. Byrareddy, Francois Villinger, Ann J. Hessell, Nancy L. Haigwood, K. Jagannadha Sastry, Michael A. Barry
JOURNAL OF VIROLOGY
(2019)
Article
Immunology
Gloria Sierra, Stephanie Dorta-Estremera, Venkatesh L. Hegde, Sita M. K. Nookala, Ananta Yanamandra, K. Jagannadha Sastry
Article
Oncology
Tatiana Karpinets, Travis N. Solley, Megan D. Mikkelson, Stephanie Dorta-Estremera, Sita S. Nookala, Andrea Y. Delgado Medrano, Joseph F. Petrosino, Melissa P. Mezzari, Jinghua Zhang, P. Andrew Futreal, K. Jagannadha Sastry, Lauren E. Colbert, Ann Klopp
CANCER PREVENTION RESEARCH
(2020)
Article
Biology
Travis T. Sims, Molly B. El Alam, Tatiana Karpinets, Stephanie Dorta-Estremera, Venkatesh L. Hegde, Sita Nookala, Kyoko Yoshida-Court, Xiaogang Wu, Greyson W. G. Biegert, Andrea Y. Delgado Medrano, Travis Solley, Mustapha Ahmed-Kaddar, Bhavana Chapman, K. Jagannadha Sastry, Melissa P. Mezzari, Joseph F. Petrosino, Lilie L. Lin, Lois Ramondetta, Anuja Jhingran, Kathleen M. Schmeler, Nadim J. Ajami, Jennifer Wargo, Lauren E. Colbert, Ann H. Klopp
Summary: The diversity of gut microbiota is associated with a favorable response to chemoradiation in cervical cancer patients. Variations in microbiota composition among patients correlate with short term and long-term survival outcomes. Analysis showed that patients with high microbiome diversity had increased tumor infiltration of certain immune cells throughout radiation therapy, suggesting a potential way to enhance treatment efficacy and outcomes.
COMMUNICATIONS BIOLOGY
(2021)
Review
Neurosciences
Paulino Ramirez, Gabrielle Zuniga, Wenyan Sun, Adrian Beckmann, Elizabeth Ochoa, Sarah L. DeVos, Bradley Hyman, Gabriel Chiu, Ethan R. Roy, Wei Cao, Miranda Orr, Virginie Buggia-Prevot, William J. Ray, Bess Frost
Summary: The study reveals that transposable elements are activated in the context of brain aging and tauopathy in mice, with endogenous retrovirus (ERV) class of retrotransposons being particularly enriched. Protein encoded by Intracisternal A-particle is found to be elevated in tau transgenic mouse brains. The research also suggests increased DNA copy number of transposable elements in the brains of tau transgenic mice, indicating potential retrotransposition activity in the context of tauopathy.
PROGRESS IN NEUROBIOLOGY
(2022)
Article
Oncology
Lauren E. Colbert, Molly B. El, Erica J. Lynn, Julianna Bronk, Tatiana Karpinets, Xiaogang Wu, Bhavana Chapman, Travis T. Sims, Daniel Lin, Ramez Kouzy, Julie Sammouri, Greyson Biegert, Andrea Y. Delgado Medrano, Adilene Olvera, K. Jagannadha Sastry, Patricia J. Eifel, Anuja Jhingran, Lilie Lin, Lois M. Ramondetta, Andrew P. Futreal, Amir A. Jazaeri, Kathleen M. Schmeler, Jingyan Yue, Aparna Mitra, Kyoko Yoshida-Court, Jennifer A. Wargo, Travis N. Solley, Venkatesh Hegde, Sita S. Nookala, Ananta Yanamandra, Stephanie Dorta-Estremera, Geena Mathew, Rohit Kavukuntla, Cassidy Papso, Mustapha Ahmed-Kaddar, Minsoo Kim, Jianhua Zhang, Alexandre Reuben, Emma B. Holliday, Bruce D. Minsky, Albert C. Koong, Eugene J. Koay, Prajnan Das, Cullen M. Taniguchi, Ann Klopp
Summary: Human papillomavirus (HPV) infection causes 600,000 new cancers worldwide each year. This study found that HPV-related cancers express tumor-specific antigens E6 and E7. The research also showed that HPV-specific T cells and receptor motifs expanded in the tumor microenvironment during treatment, and improved recurrence-free survival was associated with the expansion of HPV-responsive T cells.
CANCER IMMUNOLOGY RESEARCH
(2022)
Article
Microbiology
Jose J. Rosado-Franco, Albersy Armina-Rodriguez, Nicole Marzan-Rivera, Armando G. Burgos, Natalie Spiliopoulos, Stephanie M. Dorta-Estremera, Loyda B. Mendez, A. M. Espino
Summary: The study demonstrated that a low-dose of Fh15 can suppress the cytokine storm and other inflammatory markers during the early phase of sepsis induced in rhesus macaques, including preventing bacteremia, suppressing LPS levels in plasma, reducing the production of proinflammatory cytokines, and increasing innate immune cell populations in blood. Fh15 derived from Fasciola hepatica shows potential as an anti-inflammatory drug against sepsis in a nonhuman primate model, making it a promising candidate for drug development.
MICROBIOLOGY SPECTRUM
(2021)
Article
Immunology
Ethan R. Roy, Gabriel Chiu, Sanming Li, Nicholas E. Propson, Rupa Kanchi, Baiping Wang, Cristian Coarfa, Hui Zheng, Wei Cao
Summary: This study reveals the cellular response to type I interferon in Alzheimer's disease and suggests that IFN-I signaling plays a critical role in the neuroinflammatory network, leading to detrimental cellular states for memory and cognition.
Article
Cell Biology
Peng Zhao, Yuanzhong Xu, Lulin Jiang, Xuejun Fan, Leike Li, Xin Li, Hisashi Arase, Yingjun Zhao, Wei Cao, Hui Zheng, Huaxi Xu, Qingchun Tong, Ningyan Zhang, Zhiqiang An
Summary: This study demonstrated the feasibility of engineering multivalent TREM2 agonistic antibody coupled with TfR-mediated brain delivery to enhance microglia functions and reduce amyloid pathology in vitro and in vivo.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Editorial Material
Neurosciences
Ethan Roy, Wei Cao
MOLECULAR NEURODEGENERATION
(2022)
Article
Oncology
Jorge R. Galan-Ortiz, Kamila A. Andino del Valle, Abelardo A. Perez-Rosario, Daniel Castanon L. Pereira, Jennifer Diaz-Rivera, Pamela A. Merheb-Finianos, Stephanie M. Dorta-Estremera
Summary: B cells infiltrate HPV-positive oropharyngeal tumors, but their role during PD-1 blockade therapy needs further investigation. Using a mouse model for HPV-positive oropharyngeal cancer, B cells within tumors were characterized and their role during PD-1 blockade was determined.
FRONTIERS IN ONCOLOGY
(2023)
Article
Oncology
Anamaris Torres-Sanchez, Michael Rivera-Robles, Linette Castillo-Pichardo, Magaly Martinez-Ferrer, Stephanie M. Dorta-Estremera, Suranganie Dharmawardhane
Summary: Metastatic disease is the primary cause of mortality from epithelial cancers, especially breast cancer, and lacks effective treatments. In this study, inhibitors of Rac and Cdc42 were found to simultaneously target the migration of cancer cells and immunosuppressive inflammatory cells in the tumor microenvironment, leading to inhibition of breast cancer metastasis and growth.
FRONTIERS IN ONCOLOGY
(2023)
Editorial Material
Immunology
Stephanie M. Dorta-Estremera, Esther A. Peterson, Josue Perez-Santiago, JoAnn M. Sekiguchi
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Kyoko Yoshida-Court, Tatiana. V. V. Karpinets, Aparna Mitra, Travis. N. N. Solley, Stephanie Dorta-Estremera, Travis. T. T. Sims, Andrea. Y. Delgado Y. Medrano, Molly. B. B. El Alam, Mustapha Ahmed-Kaddar, Erica. J. J. Lynn, K. Jagannadha Sastry, Jianhua Zhang, Andrew Futreal, Alpa Nick, Karen Lu, Lauren. E. E. Colbert, Ann. H. H. Klopp
Summary: This study analyzed T cell immune-related characteristics and T cell receptor (TCR) repertoire in malignant ascites from high-grade epithelial ovarian cancer patients. Results showed that poor prognosis was associated with increased ratio of CD8+ T cells to regulatory T cells and altered TCR functions. Patients with better prognosis had TCRs that recognized cancer antigens and were enriched with TCR sequences predicted to recognize epithelial ovarian cancer antigens. A TCR motif that bound a specific neoantigen was identified and enriched in patients with excellent or good prognosis. These findings highlight the importance of effective antigen-specific immune response in ascitic fluid for a good outcome in high-grade epithelial ovarian cancer.
Article
Neurosciences
Sanming Li, Ethan R. Roy, Yanyu Wang, Trent Watkins, Wei Cao
Summary: Alzheimer's disease is a neurodegenerative disorder, and tau aggregation is closely linked to its clinical progression. This study overexpressed human tau in primary mouse neurons and observed significant axonal degeneration and cell death, providing convincing evidence for the association between tau-induced neurodegeneration and AD.
MOLECULAR NEUROBIOLOGY
(2023)