Article
Biochemical Research Methods
M. Caleb Marlin, Tayte Stephens, Christian Wright, Miles Smith, Kyle Wright, Joel M. Guthridge
Summary: Imaging mass cytometry (IMC) is a powerful spatial technology that allows for high-resolution imaging with multiple markers. However, it still faces challenges such as lower resolution and smaller regions of interest compared to other microscopy techniques. In order to overcome these limitations, the combination of H&E and multiplex immunofluorescence imaging is proposed to achieve higher resolution of structural and cellular compartments.
Article
Immunology
Marte Heimli, Siri Tennebo Flam, Hanne Sagsveen Hjorthaug, Don Trinh, Michael Frisk, Karl-Andreas Dumont, Teodora Ribarska, Xavier Tekpli, Mario Saare, Benedicte Alexandra Lie
Summary: To prevent autoimmune reactions, thymocytes expressing self-reactive T cell receptors (TCRs) are eliminated, but they may diverge into tolerogenic, agonist selected lineages. In this study, Cellular Indexing of Transcriptomes and Epitopes by sequencing (CITE-seq) and spatial transcriptomics were performed on pediatric human thymus to investigate thymocyte development and agonist selection. It was found that thymocytes expressing markers of strong TCR signaling followed a different developmental trajectory, while different agonist selected T cell populations exhibited variable timing of induction. Dendritic cells, B cells, and stromal cells were identified as contributors to agonist selection, influencing thymocytes at specific developmental stages within distinct spatial niches.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemical Research Methods
Caio Santos Bonilha
Summary: BCyto is an open-source R package/shiny app that allows cell biologists without coding skills to reliably analyze FCM data in R. It provides various useful tools and an intuitive interface for data visualization and analysis.
MOLECULAR AND CELLULAR PROBES
(2022)
Article
Medicine, Research & Experimental
Keke Liu, Yukun Guo, Qisheng You, Tristan Hormel, Thomas S. Hwang, Yali Jia
Summary: Conventional optical coherence tomography angiography has limitations in field of view, but recent improvements are exploring larger fields to enhance pathology detection. Normative measurements for key metrics like vessel density in peripheral retina are lacking. This study measured vessel density and intercapillary distance in different retinal regions and found significant differences between central macular and temporal regions, suggesting potential for identifying vascular pathology in the peripheral retina.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2021)
Article
Clinical Neurology
Florian Ingelfinger, Sinduya Krishnarajah, Michael Kramer, Sebastian G. Utz, Edoardo Galli, Mirjam Lutz, Pascale Zwicky, Ayse U. Akarca, Nicole Puertas Jurado, Can Ulutekin, David Bamert, Corinne C. Widmer, Luca Piccoli, Federica Sallusto, Nicolas G. Nunez, Teresa Marafioti, Didier Schneiter, Isabelle Opitz, Antonio Lanzavecchia, Hans H. Jung, Donatella De Feo, Sarah Mundt, Bettina Schreiner, Burkhard Becher
Summary: This study utilized high-dimensional single-cell mass and spectral cytometry to explore the immune dysregulation in Myasthenia gravis (MG) patients, identifying two dysregulated subsets of inflammatory T helper cells that were reduced in the blood of MG patients and inversely correlated with disease severity. These cellular markers rebounded in the blood of MG patients after thymus removal surgery, suggesting their potential role as markers of disease activity.
ACTA NEUROPATHOLOGICA
(2021)
Article
Immunology
Julien Verdier, Odessa-Maud Fayet, Edouard Hemery, Frederique Truffault, Natalia Pinzon, Sophie Demeret, Anthony Behin, Elie Fadel, Julien Guihaire, Aurelien Corneau, Catherine Blanc, Sonia Berrih-Aknin, Rozen Le Panse
Summary: Myasthenia Gravis (MG) is a neurological autoimmune disease characterized by muscle weaknesses caused by anti-acetylcholine receptor (AChR) autoantibodies. In this study, using mass cytometry, researchers examined PBMCs from MG patients and controls and found dysregulations in monocytes, innate lymphoid cells, and gamma delta T cells. The study also revealed the impact of the thymic environment on gamma delta T cell differentiation and the decreased activity of monocytes in MG patients.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Microbiology
Wei Jiang, Chao Li, Yanjun Li, Huadong Peng
Summary: Microbial lipids have gained significant attention in the field of metabolic engineering and synthetic biology due to their market growth and versatile applications. This review provides an overview of lipid synthesis modules in yeast and discusses various metabolic engineering strategies for enhancing lipid production. It also explores the potential impact of lipid pathway engineering on cellular physiological properties and suggests future trends for enhancing intracellular lipid production in yeast.
Article
Immunology
Benjamin Diethelm-Varela, Antonia Reyes, Yvonne Rosenstein, Jorge Kalil, Marcelo Hill, Guillermo Docena, Ignacio Anegon, Pablo A. Gonzalez, Alexis M. Kalergis
Summary: FOCIS Goes South is a course and workshop organized by FOCIS Centers of Excellence (FCEs) from across Latin America, focusing on advanced immunology, flow cytometry, as well as cutting-edge research in autoimmunity, tolerance, cancer, infectious diseases, and vaccines. The second edition of FOCIS Goes South took place virtually with over 950 registered participants due to the COVID-19 pandemic.
Article
Immunology
Camillo Bechi Genzano, Eugenia Bezzecchi, Debora Carnovale, Alessandra Mandelli, Elisa Morotti, Valeria Castorani, Valeria Favalli, Angela Stabilini, Vittoria Insalaco, Francesca Ragogna, Valentina Codazzi, Giulia Maria Scotti, Stefania Del Rosso, Benedetta Allegra Mazzi, Maurizio De Pellegrin, Andrea Giustina, Lorenzo Piemonti, Emanuele Bosi, Manuela Battaglia, Marco J. Morelli, Riccardo Bonfanti, Alessandra Petrelli
Summary: This study identified the circulating immune fingerprint of newly diagnosed pediatric T1D by analyzing flow cytometry data. It found an increase in naive CD4 T cells and plasmacytoid DCs, a decrease in CD56(bright)NK cells, and a non-selective decrease in CD3(+)CD56(+) regulatory T cells in T1D. This research provides valuable reference data for understanding the pathogenesis of T1D.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Hematology
Martin Pelletier, Yann Breton, Isabelle Allaeys, Yann Becker, Tom Benson, Eric Boilard
Summary: The study reveals that extracellular vesicles released by activated platelets contain mitochondria, which can be captured by neutrophils and monocytes, enhancing their mitochondrial function. These external mitochondria may support other metabolic functions through transcellular bioenergetics.
Article
Computer Science, Information Systems
Ali Jazayeri, Muge Capan, Julie Ivy, Ryan Arnold, Christopher C. Yang
Summary: Sepsis, a devastating multi-stage health condition with high mortality rate, has attracted attention from data science and machine learning communities. Analyzing data from adult patients, significant proximate failures of cellular and physiological responses were found to be better in predicting outcomes, which can inform clinical practices for better patient prediction and improvement.
IEEE JOURNAL OF BIOMEDICAL AND HEALTH INFORMATICS
(2021)
Article
Biochemical Research Methods
Prabina Kumar Meher, Anil Rai, Atmakuri Ramakrishna Rao
Summary: This study introduces a novel computational tool for predicting the multiple localization of mRNAs. Based on the proposed approach, an online prediction server mLoc-mRNA is accessible at http://cabgrid.res.in:8080/mlocmrna/. The developed approach is believed to supplement the existing tools and techniques for the localization prediction of mRNAs.
BMC BIOINFORMATICS
(2021)
Article
Biotechnology & Applied Microbiology
Sonali Nath, Debashis Patra, Anish Nag, Rita Kundu
Summary: This study aims to identify the most potent phytocompound present in the extract of Curcuma zanthorrhiza rhizome and determine its primary mode of action against HPV16-positive SiHa cervical cancer cells. The extract was found to inhibit cell proliferation in a dose-dependent manner and induce cytotoxicity through dysregulation of antioxidant defense system and activation of the PI3K/AKT/mTOR pathway, leading to autophagic cell death. This is the first report of Curcuma zanthorrhiza Roxb. inducing autophagy.
Article
Biochemistry & Molecular Biology
Albert Truong, Dru Myerscough, Ian Campbell, Joshua Atkinson, Jonathan J. Silberg
Summary: This study shows that flavodoxins (Flds) can support electron transfer to a ferredoxin-dependent sulfite reductase (SIR), and the pattern of insertion tolerance is influenced by interactions with oxidoreductase partners.
Article
Surgery
Tina Schmidt, Verena Klemis, David Schub, Sophie Schneitler, Matthias C. Reichert, Heinrike Wilkens, Urban Sester, Martina Sester, Janine Mihm
Summary: This study found that solid organ transplant recipients had lower levels of SARS-CoV-2-specific antibodies and T cells compared to controls after homologous or heterologous vaccination. However, heterologous vaccination showed promise in improving immune responses in transplant recipients, with higher induction of antibodies and CD4 T cells.
AMERICAN JOURNAL OF TRANSPLANTATION
(2021)
Article
Immunology
Dominic P. Golec, Laura M. Henao Caviedes, Troy A. Baldwin
JOURNAL OF IMMUNOLOGY
(2016)
Article
Immunology
Dominic P. Golec, Romy E. Hoeppli, Laura M. Henao Caviedes, Jillian McCann, Megan K. Levings, Troy A. Baldwin
JOURNAL OF EXPERIMENTAL MEDICINE
(2017)
Article
Immunology
Qian Nancy Hu, Alexander Y. W. Suen, Laura M. Henao Caviedes, Troy A. Baldwin
JOURNAL OF IMMUNOLOGY
(2017)
Article
Immunology
Mengqi Dong, Patricio Artusa, Stephanie A. Kelly, Marilaine Fournier, Troy A. Baldwin, Judith N. Mandl, Heather J. Melichar
JOURNAL OF IMMUNOLOGY
(2017)
Article
Oncology
Brittany A. Umer, Ryan S. Noyce, Brian C. Franczak, Mira M. Shenouda, Rees G. Kelly, Nicole A. Favis, Megan Desaulniers, Troy A. Baldwin, Mary M. Hitt, David H. Evans
CANCER IMMUNOLOGY RESEARCH
(2020)
Article
Immunology
Kevin Tsai, Yu-Hsuan Huang, Caixia Ma, Troy A. Baldwin, Kenneth W. Harder, Bruce A. Vallance, John J. Priatel
JOURNAL OF IMMUNOLOGY
(2020)
Article
Neurosciences
Wentong Long, Mohammad Fatehi, Shubham Soni, Rashmi Panigrahi, Koenraad Philippaert, Yi Yu, Rees Kelly, Brett Boonen, Amy Barr, Dominic Golec, Scott A. Campbell, Katarina Ondrusova, Matt Hubert, Troy Baldwin, M. Joanne Lemieux, Peter E. Light
JOURNAL OF PHYSIOLOGY-LONDON
(2020)
Article
Hematology
Olga Mykhailova, Tracey R. Turner, Carly Olafson, Anita Howell, Susan N. Nahirniak, Juanita Wizniak, Hanan Y. N. Gerges, Troy Baldwin, Gwen Clarke, Jason P. Acker
Summary: Irradiation of RBCs can effectively inactivate T lymphocytes to prevent TA-GVHD. Leukoreduction and storage of blood products may be an alternative prevention strategy. Viability of residual WBCs in stored RBCs remains high, although their proliferative ability decreases over time.
Article
Immunology
Patrick L. Stemkowski, Nataliya Bukhanova-Schulz, Troy Baldwin, Elena Posse de Chaves, Peter A. Smith
Summary: Peripheral nerve injury often leads to chronic neuropathic pain, which is initiated by an inflammatory response that causes persistent excitation of neurons. This study reveals the impact of 1aM IL-1β on the excitability of neurons, potentially mediated through type 1 interleukin receptors.
JOURNAL OF NEUROIMMUNOLOGY
(2021)
Article
Immunology
Kevin Joannou, Dominic P. Golec, Haiguang Wang, Laura M. Henao-Caviedes, Julia F. May, Rees G. Kelly, Rigel Chan, Stephen C. Jameson, Troy A. Baldwin
Summary: This study investigates the relationship between maturation and thymic export of adult-derived gamma delta thymocytes in mice. The findings reveal the existence of two populations of gamma delta T cell recent thymic emigrants (RTEs) in adult mice, with one population maturing outside the thymus and the other population completing maturation within the thymus. Additionally, a heterogeneous population of resident gamma delta thymocytes of unknown functional importance was identified.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Cell Biology
Jhon R. Enterina, Susmita Sarkar, Laura Streith, Jaesoo Jung, Britni M. Arlian, Sarah J. Meyer, Hiromu Takematsu, Changchun Xiao, Troy A. Baldwin, Lars Nitschke, Mark J. Shlomchick, James C. Paulson, Matthew S. Macauley
Summary: Germinal centers are critical for antibody affinity maturation, and glycan remodeling plays a key role in maintaining B cells in the germinal center. Downregulation of CD22 ligands on B cells in the germinal center leads to increased apoptosis, reduced production of memory B cells and plasma cells, and delayed affinity maturation of antibodies. These defects are CD22 dependent.
Review
Immunology
Kevin Joannou, Troy A. Baldwin
Summary: The immune system consists of various T-cell lineages distributed in both lymphoid and non-lymphoid tissues. The intestinal epithelium contains intraepithelial lymphocytes that play a crucial role in maintaining barrier homeostasis. This review focuses on T-cell receptor alpha beta (TCR alpha beta) CD8 alpha alpha intraepithelial lymphocytes, discussing their development and functions in the intestines. The available evidence suggests an ontogenic story starting from agonist selection of T cells in the thymus and ending with the specific signaling environment of the intestinal epithelium. The development of different waves of TCR alpha beta CD8 alpha alpha intraepithelial lymphocytes and their importance for intestinal epithelial homeostasis raise further key questions.
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
(2023)
Article
Immunology
Timothy N. Friedman, Olivia La Caprara, Celine Zhang, Kelly Lee, Julia May, Christian A. Faig, Troy Baldwin, Jason R. Plemel, Anna M. W. Taylor, Bradley J. Kerr
Summary: Decades of research have enhanced our understanding of the cellular mechanisms underlying chronic pain, but the biological variable of sex has been overlooked, limiting the clinical application of these breakthroughs. This study investigates the differences in chronic pain between male and female mice resulting from inflammatory activation of the innate immune system. The findings suggest that sex differences in cellular mechanisms may lead to more targeted approaches for clinical applications.
BRAIN BEHAVIOR AND IMMUNITY
(2023)
Meeting Abstract
Immunology
Adeolu Adegoke, John R. Sedy, Carl F. Ware, Troy A. Baldwin, Colin C. Anderson
JOURNAL OF IMMUNOLOGY
(2022)
Meeting Abstract
Immunology
M. Dong, P. Artusa, S. A. Kelly, M. Fournier, T. A. Baldwin, J. N. Mandl, H. J. Melichar
EUROPEAN JOURNAL OF IMMUNOLOGY
(2019)