Article
Hematology
Adeline Blandinieres, Anna M. Randi, Koralia E. Paschalaki, Coralie L. Guerin, Juan M. Melero-Martin, David M. Smadja
Summary: The study aims to provide expert consensus guidance on the isolation and culture of endothelial colony-forming cells (ECFCs). A survey was conducted among 21 experts from 10 different countries to attain a consensus on ECFC isolation and culture. The results indicate the need for standardization and validation of ECFC isolation and culture procedures for clinical laboratory practice.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Hematology
Gregoire Detriche, Nicolas Gendron, Aurelien Philippe, Maxime Gruest, Paul Billoir, Elisa Rossi, Coralie L. Guerin, Anna Lokajczyk, Severine Brabant, Dominique Prie, Tristan Mirault, David M. Smadja
Summary: Gonadotropins increase ECFC-related angiogenesis and may be involved in thrombin generation in cardiovascular disease. Gonadotropins may act as biomarkers; moreover, we hypothesize that gonadotropin-blocking strategies may be a novel interesting therapeutic approach in atherosclerotic cardiovascular disease.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Endocrinology & Metabolism
Shauni Loopmans, Ingrid Stockmans, Geert Carmeliet, Steve Stegen
Summary: This article describes a comprehensive procedure to isolate and culture skeletal stem and progenitor cells (SSPCs) from the metaphysis and endosteum. In vitro, metaphyseal/endosteal SSPCs possess self-renewing capacity and multipotency. Expanding metaphyseal/endosteal SSPCs under low oxygen conditions enhances their proliferation capacity while maintaining their progenitor properties.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Simon Toupance, Stephanie Simonici, Carlos Labat, Chloe Dumoulin, Tsung-Po Lai, Cecile Lakomy, Veronique Regnault, Patrick Lacolley, Francoise Dignat George, Florence Sabatier, Abraham Aviv, Athanase Benetos
Summary: The study found that individuals with longer telomeres have more self-renewing endothelial colony-forming cells. White blood cell telomere length can serve as a surrogate marker for endothelial repair capacity in clinical and laboratory practice, with potential applications.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2021)
Article
Medicine, General & Internal
Nadia Meyer, Lars Brodowski, Katja Richter, Constantin S. von Kaisenberg, Bianca Schroeder-Heurich, Frauke von Versen-Hoeynck
Summary: Pravastatin enhances the functional capacities of ECFCs and promotes angiogenic signaling and protein expression, improving endothelial health. These effects may offer a promising option for ameliorating endothelial dysfunction and understanding the pleiotropic function of statins.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Cell Biology
Suneel Rallapalli, Soma Guhathakurta, Purna S. Korrapati
Summary: The discovery of CPCs has raised hopes for heart cell therapy, but there are still issues with defining CPC types and validating methods for generating high-quality CPC populations. Long-term culture may alter CPC immunophenotypes, highlighting the need for validated molecular signatures to assess the impact of ex vivo expansion on cell therapy products.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Atanu Sen, Himani Thakkar, Vinnyfred Vincent, Sandeep Rai, Archna Singh, Sujata Mohanty, Ambuj Roy, Lakshmy Ramakrishnan
Summary: This study investigated the relationship between biopterins levels of endothelial colony forming cells (ECFCs) and endothelial progenitor cells (EPCs) in circulation, as well as the functional potential of ECFCs. The results suggest that biopterins play an important role in maintaining the functional integrity of EPCs and ECFCs in circulation.
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Stephanie Simoncini, Simon Toupance, Carlos Labat, Sylvie Gautier, Chloe Dumoulin, Laurent Arnaud, Maria G. Stathopoulou, Sophie Visvikis-Siest, Pascal M. Rossi, Athanase Benetos, Francoise Dignat-George, Florence Sabatier
Summary: This study investigates the relationship between atherosclerotic cardiovascular disease (ASCVD), inflammation markers, and circulating endothelial progenitor cells. The findings suggest that ASCVD impairs the colony forming capacity and angiogenic properties of endothelial colony forming cells (ECFC) in a severity-dependent manner. Additionally, alteration of ECFC is associated with increased senescent phenotype and IL-6 levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Ana Paula Toledo Del Rio, Jessica O. Frade-Guanaes, Stephanie Ospina-Prieto, Bruno K. L. Duarte, Manoel Barros Bertolo, Margareth C. Ozelo, Zoraida Sachetto
Summary: This study found that ECFCs from patients with PR3-positive GPA in remission demonstrated early losses of tube formation and reduced migration capacity, suggesting impairment of endothelial function.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Article
Multidisciplinary Sciences
Chang-Hyun Gil, Dibyendu Chakraborty, Cristiano P. Vieira, Nutan Prasain, Sergio Li Calzi, Seth D. Fortmann, Ping Hu, Kimihiko Banno, Mohamed Jamal, Chao Huang, Micheli S. Sielski, Yang Lin, Xinxin Huang, Mariana D. Dupont, Jason L. Floyd, Ram Prasad, Ana Leda F. Longhini, Trevor J. McGill, Hyung-Min Chung, Michael P. Murphy, Darrell N. Kotton, Michael E. Boulton, Mervin C. Yoder, Maria B. Grant
Summary: Human induced pluripotent stem cells (hiPSCs) can differentiate into specific mesoderm cells and restore vascular dysfunction in diabetic mice.
Review
Biochemistry & Molecular Biology
Amankeldi A. Salybekov, Shuzo Kobayashi, Takayuki Asahara
Summary: This article discusses the challenges and issues surrounding the use of endothelial progenitor cells (EPCs) as candidate cell sources for revascularization strategies. It also highlights the origins, sources, isolation, and identification techniques of EPCs, as well as the diversity of human and murine EPC markers. Furthermore, it summarizes the current and future prospects of the crosstalk between EPCs and the biology of hematopoietic cells, as well as culture techniques in the context of regeneration-associated cells (RACs).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
June Seok Heo, Sinyoung Kim
Summary: This study found that human adipose mesenchymal stem cell-derived exosomes have anti-inflammatory and pro-angiogenic effects. Exosomes inhibit inflammation by activating ROCK1 and PTEN expression, and promote angiogenesis by releasing miR-132 and miR-146a.
SCIENTIFIC REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Klara Komici, Angelica Perna, Aldo Rocca, Leonardo Bencivenga, Giuseppe Rengo, Germano Guerra
Summary: Rheumatoid Arthritis is a chronic autoimmune inflammatory disease that can lead to endothelial damage and dysfunction. Endothelial Progenitor Cells play a crucial role in vascular homeostasis and repair, but there are conflicting results regarding their number and function in RA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Paul Billoir, Adeline Blandinieres, Nicolas Gendron, Richard Chocron, Sven Gunther, Aurelien Philippe, Coralie L. Guerin, Dominique Israel-Biet, David M. Smadja
Summary: This study investigated hypercoagulability states in ECFCs from patients with IPF, finding higher thrombin potential in IPF-ECFCs and decreased expression of anticoagulant proteins on ECFCs membrane. This new finding suggests a potential role of ECFC in IPF pathophysiology and thrombotic-related disorders.
STEM CELL REVIEWS AND REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Paolo Armanetti, Anastasia Chilla, Francesca Margheri, Alessio Biagioni, Luca Menichetti, Giancarlo Margheri, Fulvio Ratto, Sonia Centi, Francesca Bianchini, Mirko Severi, Rita Traversi, Daniele Bani, Matteo Lulli, Tommaso Del Rosso, Alessandra Mocali, Elisabetta Rovida, Mario Del Rosso, Gabriella Fibbi, Anna Laurenzana
Summary: This study demonstrated that endothelial colony forming cells can effectively deliver gold nanoparticles to tumors, showing potential in cancer treatment. The AuNP-loaded ECFCs were able to migrate to tumor sites in a mouse model and remain in the tumor mass for an extended period without causing damage to healthy tissue. Additionally, the study showed that the ECFC-loaded AuNPs were efficiently cleared by the liver over time.