期刊
GENES
卷 1, 期 3, 页码 484-494出版社
MDPI AG
DOI: 10.3390/genes1030484
关键词
meiosis; chromosome; cohesin; PDS5; synaptonemal complex
资金
- Uehara Memorial Foundation
- Japan Society for the Promotion of Science
- Swedish Cancer Society
- Swedish Research Council
- Torsten och Ragnar Soderbergs Stiftelser
- Karolinska Institutet
During the first meiotic prophase, the cohesin complex is localized to the chromosome axis and contributes to chromosome organization, pairing, synapsis, and recombination. The PDS5 protein, an accessory factor of the cohesin complex, is known to be a component of meiotic chromosome cores in fungi and to be implicated in meiotic chromosome structure and function. We found by immunoblotting experiments that a mammalian PDS5 protein, PDS5B, is abundantly expressed in mouse testis compared to other tissues. Immunofluorescence labeling experiments revealed that PDS5B is highly expressed in spermatogonia and that most PDS5B is depleted from chromatin as cells enter meiosis. During the first meiotic prophase, PDS5B associates with the axial cores of chromosomes. The axial association of PDS5B was observed also in the absence of synaptonemal complex proteins, such as SYCP1 and SYCP3, suggesting that PDS5B is an integral part of the chromosome axis as defined by the cohesin complex. These results suggest that PDS5B modulates cohesin functions in spermatocytes as well as in spermatogonia, contributing to meiotic chromosome structure and function.
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