Article
Biochemistry & Molecular Biology
Shengfu Zhong, Huai Yang, Chen Chen, Tianheng Ren, Zhi Li, Feiquan Tan, Peigao Luo
Summary: The lack of available leaf color mutants in crops has hindered the understanding of photosynthesis mechanisms and limited improvements in crop yield through enhanced photosynthetic efficiency. This study identifies a temperature-sensitive albino mutant, CN19M06, which produces chlorophyll-deficient leaves at temperatures below 10 degrees C. Genetic analysis suggests that the albino phenotype is controlled by a recessive nuclear gene, TSCA1, and molecular linkage analysis narrows down its location to a specific region of chromosome 2AL. The putative candidate gene for TSCA1, TraesC-S2A01G487900, is related to chlorophyll metabolism and temperature sensitivity. Overall, CN19M06 holds great potential for studying the molecular mechanism of photosynthesis and monitoring temperature changes in wheat production.
Editorial Material
Cell Biology
Sebastian J. Hofer, YongTian Liang, Andreas Zimmermann, Sabrina Schroeder, Jorn Dengjel, Guido Kroemer, Tobias Eisenberg, Stephan J. Sigrist, Frank Madeo
Summary: Spermidine is a natural polyamine that plays a central role in promoting cellular autophagy, prolonging lifespan, and improving mitochondrial function. Dietary supplementation of spermidine enhances the hypusination of protein eEF5/EIF5A, boosts cerebral mitochondrial function and cognition.
Article
Cell Biology
Eriko Hamasaki, Natsuki Wakita, Hiroki Yasuoka, Hikaru Nagaoka, Masayuki Morita, Eizo Takashima, Takayuki Uchihashi, Tetsuya Takeda, Tadashi Abe, Ji-Won Lee, Tadahiro Iimura, Moin A. Saleem, Naohisa Ogo, Akira Asai, Akihiro Narita, Kohji Takei, Hiroshi Yamada
Summary: This study investigates the molecular mechanisms underlying the regulation of actin by dynamin in human podocytes. The results suggest that the affinity of the K562E mutant for lipid membranes and its atypical self-assembling properties lead to actin disorganization in podocytes. Furthermore, the interaction between actin and membranes mediated by dynamin is critical for actin bundle formation and podocyte morphology and functions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Neurosciences
Ceazar Nave, Logan Roberts, Patrick Hwu, Jerson D. Estrella, Thanh C. Vo, Thanh H. Nguyen, Tony Thai Bui, Daniel J. Rindner, Nicholas Pervolarakis, Paul J. Shaw, Tanya L. Leise, Todd C. Holmes
Summary: The study developed a method for imaging oscillations in fruit fly brain cells and found that weekend light shifts can disrupt neuronal synchrony and rhythmicity. Weekend light shifts were shown to cause desynchronization in circadian neuronal oscillators, leading to observed behavioral defects in sleep, learning, and memory.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Michael Marquis, Rachel I. Wilson
Summary: Activity of dopamine neurons in different regions and cell types of the Drosophila brain is associated with learning, locomotion, and odor. These findings suggest a potential role of synaptic plasticity in dopamine neuron function.
Article
Biology
Jianjian Zhao, Xuchen Zhang, Bohan Zhao, Wantong Hu, Tongxin Diao, Liyuan Wang, Yi Zhong, Qian Li
Summary: Animals have strategies to cope with proactive and retroactive interference when learning different tasks consecutively. In this study, researchers investigated the molecular mechanisms of proactive and retroactive interference in Drosophila. They found that proactive interference is more sensitive to the inter-task interval (ITI) and that manipulating the protein tyrosine phosphatase SHP2 affects proactive interference but not retroactive interference or single learning tasks. These findings suggest that sequential learning of different tasks triggers distinct molecular mechanisms to tune proactive and retroactive interference.
Article
Biochemistry & Molecular Biology
Ergina Vourkou, Vassilis Paspaliaris, Anna Bourouliti, Maria-Christina Zerva, Engie Prifti, Katerina Papanikolopoulou, Efthimios M. C. Skoulakis
Summary: The accumulation of highly post-translationally modified tau proteins is a hallmark of tauopathies, including Alzheimer's disease. Different isoforms of tau have distinct distributions in the brain and are involved in different tauopathies. In a Drosophila study, expression of the six human brain tau isoforms showed isoform-specific effects on neuronal dysfunction, vulnerability to oxidative stress, and life span, suggesting the importance of functional differentiation of tau isoforms in tauopathies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Peng Hu, Yi Wen, Yueying Wang, Hao Wu, Junge Wang, Kaixiong Wu, Bingze Chai, Lixin Zhu, Guangheng Zhang, Zhenyu Gao, Deyong Ren, Li Zhu, Longbiao Guo, Dali Zeng, Jing Xu, Song Yan, Qian Qian, Yuchun Rao, Jiang Hu
Summary: The study identified that under low temperature conditions, mutations in the SCR8 gene may activate plant defense response, leading to a crown root growth defect.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Infectious Diseases
Daniel J. Wolter, Alison Scott, Catherine R. Armbruster, Dale Whittington, John S. Edgar, Xuan Qin, Anne Marie Buccat, Sharon McNamara, Marcella Blackledge, Adam Waalkes, Stephen J. Salipante, Robert K. Ernst, Lucas R. Hoffman
Summary: This study describes the discovery and investigation of an antibiotic-dependent, temperature-sensitive Pseudomonas aeruginosa mutant in the respiratory tract of a CF patient, highlighting the clinical challenges bacterial adaptations can present, including dependency on antibiotics and limitations of laboratory detection methods.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Olga G. Zatsepina, Lyubov N. Chuvakova, Ekaterina A. Nikitina, Alexander P. Rezvykh, Alexey S. Zakluta, Svetlana V. Sarantseva, Nina V. Surina, Alexander L. Ksenofontov, Ludmila A. Baratova, Viktoria Y. Shilova, Michael B. Evgen'ev
Summary: This study investigated the role of hydrogen sulfide (H2S) in learning and memory processes by examining the effects of gene deletions in Drosophila melanogaster. The results showed that the deletion of the CBS gene completely blocked short- and long-term memory formation, while the deletion of the CSE gene impaired long-term memory retention. Transcriptome profiling revealed down-regulation of genes involved in learning and memory, reproductive behavior, cognition, and the oxidation-reduction process in strains with CBS deletion, highlighting the importance of H2S production in these vital processes.
Article
Biochemistry & Molecular Biology
Na Lv, Ying Wang, Yongfeng Liu, Jiaming Tang, Qiang Lei, Yizhuo Wang, Hongen Wei
Summary: This study observed the effects of Pax2 gene deletion on learning and memory ability and neural structure, and used single-cell transcriptome sequencing to reveal possible molecular mechanisms.
ACS CHEMICAL NEUROSCIENCE
(2022)
Article
Multidisciplinary Sciences
Jia Jia, Lei He, Junfei Yang, Yichun Shuai, Jingjing Yang, Yalan Wu, Xin Liu, Tianli Chen, Guaxiu Wang, Xingyu Wang, Xiaoxu Song, Zhaowen Ding, Yan Zhu, Li Zhang, Peng Chen, Hongtao Qin
Summary: Chronic stress can induce severe cognitive impairments in Drosophila melanogaster, with long-lasting learning and memory deficits accompanied by depression-like behaviors. Excessive dopaminergic activity is shown to increase susceptibility to chronic stress-induced learning deficits, with specific dopaminergic neurons and mushroom body output neurons playing key roles in regulating this susceptibility. Imaging studies suggest that dopaminergic activity is essential for the development of maladaptations in the mushroom body network induced by chronic stress, leading to CSLD.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
Carlotta Pribbenow, Yi-Chun Chen, M-Marcel Heim, Desiree Laber, Silas Reubold, Eric Reynolds, Isabella Balles, Tania Fernandez-d Alquicira, Raquel Suarez-Grimalt, Lisa Scheunemann, Carolin Rauch, Tanja Matkovic, Joerg Roesner, Gregor Lichtner, Sridhar R. Jagannathan, David Owald
Summary: This study found that there is postsynaptic plasticity at cholinergic output synapses in the Drosophila mushroom bodies, which is similar to the memory formation mechanism in vertebrates. The nicotinic acetylcholine receptor subunit alpha 5 plays a key role in appetitive memory induction, while the alpha 2 subunits mediate memory expression.
Review
Entomology
Thu N. M. Nguyen, Amanda Choo, Simon W. Baxter
Summary: The sterile insect technique (SIT) is a pest control strategy that involves mass-rearing, sterilization, and release of male insects to reduce the population of insects that pose threats to agriculture or human health. Developing genetic sexing strains with conditional temperature sensitive lethal mutations offers potential to eliminate female embryos through heat treatment. The challenge remains in developing robust sex-separation systems for SIT programs to improve efficiency and cost-effectiveness.
Article
Entomology
Md Zeeshan Ali, Anushree Anushree, Anwar L. Bilgrami, Aarif Ahsan, Mohammad Shamsul Ola, Rizwanul Haque, Jawaid Ahsan
Summary: Phenylacetaldehyde (PAH), an aromatic odorant, serves as an important food and courtship cue for adult fruit flies. Our study found that larvae are attracted to PAH in a dose-dependent manner and can be trained with PAH as a marker for positive and negative reinforcement. Unlike adult flies, larvae primarily sense PAH through odorant receptors. This study provides valuable insights into how larvae perceive and process PAH odorant at the neural level.
JOURNAL OF INSECT SCIENCE
(2023)
Article
Cell Biology
Samantha J. Hindle, Roeben N. Munji, Elena Dolghih, Garrett Gaskins, Souvinh Orng, Hiroshi Ishimoto, Allison Soung, Michael DeSalvo, Toshihiro Kitamoto, Michael J. Keiser, Matthew P. Jacobson, Richard Daneman, Roland J. Bainton
Article
Neurosciences
Daichi Yamada, Hiroshi Ishimoto, Xiaodong Li, Tsunehiko Kohashi, Yuki Ishikawa, Azusa Kamikouchi
JOURNAL OF NEUROSCIENCE
(2018)
Article
Multidisciplinary Sciences
Hiroshi Ishimoto, Hiroko Sano
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2018)
Article
Genetics & Heredity
Junko Kasuya, Atulya Iyengar, Hung-Lin Chen, Patrick Lansdon, Chun-Fang Wu, Toshihiro Kitamoto
JOURNAL OF NEUROGENETICS
(2019)
Article
Biochemistry & Molecular Biology
Hiroshi Ishimoto, Azusa Kamikouchi
Article
Genetics & Heredity
Emily Petruccelli, Arianna Lark, James A. Mrkvicka, Toshihiro Kitamoto
JOURNAL OF NEUROGENETICS
(2020)
Article
Neurosciences
Show Inami, Shoma Sato, Shu Kondo, Hiromu Tanimoto, Toshihiro Kitamoto, Takaomi Sakai
JOURNAL OF NEUROSCIENCE
(2020)
Article
Genetics & Heredity
Hung-Lin Chen, Junko Kasuya, Patrick Lansdon, Garrett Kaas, Hanxi Tang, Maggie Sodders, Toshihiro Kitamoto
G3-GENES GENOMES GENETICS
(2020)
Article
Biochemistry & Molecular Biology
Daniel White, Raquel P. de Sousa Abreu, Andrew Blake, Jeremy Murphy, Shardae Showell, Toshihiro Kitamoto, Hakeem O. Lawal
NEUROCHEMISTRY INTERNATIONAL
(2020)
Review
Biochemistry & Molecular Biology
Hiroshi Ishimoto, Azusa Kamikouchi
Summary: The brain integrates multiple information sources during courtship to make a final decision on mating, with research on this complex behavior being conducted through genetic manipulation and analyses in Drosophila model experimental system. While much knowledge has been accumulated on genes and neural circuits controlling sexual behaviors in male flies, research progress on mechanisms in female flies has been slower. Focus is given in this review to pre-mating behavior in female Drosophila melanogaster, exploring the neural and molecular mechanisms behind sensory integration and behavior expression towards courting males.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Show Inami, Tomohito Sato, Yuto Kurata, Yuki Suzuki, Toshihiro Kitamoto, Takaomi Sakai
Summary: The developmental regulator Ap plays dual roles in memory consolidation in clock neurons and memory maintenance in MBs in Drosophila, indicating its unique function in the neurobiological processes of LTM.
Article
Multidisciplinary Sciences
Taro Ko, Hiroki Murakami, Azusa Kamikouchi, Hiroshi Ishimoto
Summary: Lactic acid bacteria (LAB) have multiple influences on host brain function through the production of active metabolites in the gut. However, little is known about the biogenic effects of LAB on host brain function. In this study, we found that the Lactobacillus plantarum SBT2227 promoted sleep in Drosophila melanogaster and the involvement of neuropeptide F in this sleep-promoting action.
Article
Neurosciences
Junko Kasuya, Wayne Johnson, Hung -Lin Chen, Toshihiro Kitamoto
Summary: Dietary modifications have a significant impact on the expression of neurological phenotypes caused by genetic defects. Supplementing the diet with milk whey or milk lipids can effectively suppress hyperexcitable phenotypes in Drosophila mutants, with alpha-linolenic acid playing a contributing role. Lipid supplementation can modify neural development to compensate for genetic defects. Our findings lay the foundation for further research on the mechanisms by which dietary lipids modify abnormalities in neural development.
Review
Biochemistry & Molecular Biology
Arianna Lark, Toshihiro Kitamoto, Jean-Rene Martin
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2017)