期刊
CHEMELECTROCHEM
卷 1, 期 11, 页码 1854-1858出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/celc.201402098
关键词
antibiotics; biofuel cells; biosensors; self-powered sensors; sulfonamide
资金
- European Commission [FP7-PEOPLE-2013-ITN 607793]
- Cluster of Excellence RESOLV - Deutsche Forschungsgemeinschaft [EXC 1069]
- Bundesministerium fur Bildung und Forschung (BMBF) [03IS2201F]
- Spanish Ministry of Education
A biofuel cell consisting of a self-powered sulfonamide immunosensor as biocathode and a cellobiose dehydrogenase (CDH)-based bioanode was developed for the determination of sulfonamide antibiotics in milk. A graphite-rod electrode was modified with proteinG for the immobilization of selective capture antibodies. A direct competitive immunoassay with a horseradish-peroxidase-labeled analog of the antibiotic and the 2,2-azino-bis(3-ethyl benzothiazoline-6-sulfonic acid) diammonium salt-mediated reduction of H2O2 allows quantification of antibiotic residues. CDH was co-immobilized with a toluidine-blue-modified redox polymer on a graphite electrode for the biocatalytic oxidation of lactose in milk. An open-circuit voltage of 676mV and a maximal power density of 6.9 mu Wcm(-2) were obtained. The power densities measured at 550mV (vs. the anode) as a function of antibiotic concentration in milk samples allowed the construction of a calibration curve with a detection limit for sulfapyridine as low as 2.4ngmL(-1).
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