☆ 4.5 Article

Molecular Diagnosis of Shrimp Allergy: Efficiency of Several Allergens to Predict Clinical Reactivity

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY-IN PRACTICE (2015)

期刊

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY-IN PRACTICE

卷 3, 期 4, 页码 521-+

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ELSEVIER

DOI: 10.1016/j.jaip.2015.02.001

关键词

Component-resolved diagnosis; Shellfish allergy; Epitope; Microarray; DBPCFC; Tropomyosin; Arginine kinase; Myosin light chain; Sarcoplasmic calcium-binding protein; Hemocyanin; Troponin C; Fatty-acid-binding protein

类别

Allergy Immunology

资金

Food Allergy Research and Education
Roslyn Jaffe Food Allergy Institute, Icahn School of Medicine at Mount Sinai, NY

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摘要

BACKGROUND: The diagnosis of shellfish allergy remains a challenge for clinicians. Several shellfish allergens have been characterized and their IgE epitopes identified. However, the clinical relevance of this sensitization is still not clear. OBJECTIVE: The objective of this study was to identify allergens and epitopes associated with clinical reactivity to shrimp. METHODS: Shrimp-sensitized subjects were recruited and grouped based on the history of shrimp-allergic reactions and challenge outcome. IgE reactivity to recombinant crustacean allergens, and IgE and IgG4 reactivity to peptides were determined. Subjects sensitized to dust mites and/or cockroach without shrimp sensitization or reported allergic reactions, as well as nonatopic individuals, were used as controls. RESULTS: A total of 86 subjects were recruited with a skin prick test to shrimp; 74 reported shrimp-allergic reactions, 58 were allergic (38 positive double-blind placebo-controlled food challenge and 20 recent anaphylaxis), and 16 were tolerant. All subjects without a history of reactions had negative challenges. The individuals with a positive challenge more frequently recognized tropomyosin and sarcoplasmic calcium-binding proteins than those found tolerant by the challenge. Especially a sarcoplasmic-calcium-binding-protein positive test is very likely to result in a positive challenge, though the frequency of recognition is low. Subjects with dust mite and/or cockroach allergy not sensitized to shrimp recognized arginine kinase and hemocyanin. Several epitopes of these allergens may be important in predicting clinical reactivity. CONCLUSION: Tropomyosin and sarcoplasmic-calciumbinding- protein sensitization is associated with clinical reactivity to shrimp. Myosin light chain testing may help in the diagnosis of clinical reactivity. Arginine kinase and hemocyanin appear to be cross-reacting allergens between shrimp and arthropods. Detection of IgE to these allergens and some of their epitopes may be better diagnostic tools in the routine workup of shrimp allergy. (C) 2015 American Academy of Allergy, Asthma & Immunology

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Comprehending the allergen repertoire of shrimp for precision molecular diagnosis of shrimp allergy

Christine Y. Y. Wai, Nicki Y. H. Leung, Agnes S. Y. Leung, Sai Ming Ngai, Punchama Pacharn, Yat Sun Yau, Jaime Sou Da Rosa Duque, Mike Y. W. Kwan, Orathai Jirapongsananuruk, Wai Hung Chan, Gilbert T. Chua, Qun Ui Lee, Surapon Piboonpocanun, Po Ki Ho, Joshua S. C. Wong, Shanshan Li, Kary J. Y. Xu, Gary W. K. Wong, Ka Hou Chu, Patrick S. C. Leung, Pakit Vichyanond, Ting Fan Leung

Summary: The clinical management of shrimp allergy is limited by the lack of accurate tests. In this study, the allergen repertoire of shrimp was comprehensively investigated, and the sensitization pattern and diagnostic value of these allergens in allergic subjects from different populations were compared using molecular diagnosis.

ALLERGY (2022)