Article
Cell Biology
Jangham Jung, Jaehun Kim, Tae-Lin Huh, Myungchull Rhee
Summary: TRIM46 is a RING finger E3 ligase that plays a role in neuronal polarity and axon specification. The zebrafish homologue trim46a is regulated by transcription factor Foxa2 and affects the development of the midbrain and MHB. Inhibition of SHH signaling pathway by cyclopamine leads to developmental defects in midbrain and MHB through trim46a regulation.
ANIMAL CELLS AND SYSTEMS
(2021)
Article
Cell Biology
Dongmei Tang, Yitong Lu, Na Zuo, Renchun Yan, Cheng Wu, Lijuan Wu, Shaofeng Liu, Yingzi He
Summary: This study demonstrates the important role of Kdm6b in the development of the zebrafish hearing organ, regulating the migration of primordium and deposition of neuromasts through mediating the gene expression of chemokines and Wnt and Fgf signaling pathways. Targeting Kdm6b may present as a novel therapeutic regimen for hearing disorders.
CELL BIOLOGY AND TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Luis Puelles, Matias Hidalgo-Sanchez
Summary: This essay examines the molecular evidence for the existence of the 'preisthmus' in vertebrates, focusing on the mouse. Through gene expression mappings, the study identifies consistent positive markers and negative markers at different embryonic and postnatal stages. The essay argues that the unique molecular and structural profile of the preisthmus is influenced by its proximity to the isthmic organizer and the presence of high levels of FGF8 and WNT1 morphogens. The study also confirms the specific regions and neuronal types derived from the preisthmus in the adult brain.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Tanya L. Brown, Emma C. Horton, Evan W. Craig, Camille E. A. Goo, Erik C. Black, Madeleine N. Hewitt, Nathaniel G. Yee, Everett T. Fan, David W. Raible, Jeffrey P. Rasmussen, Marianne E. Bronner
Summary: This study discovered Merkel cells in zebrafish epidermis and identified Atoh1a as a marker for zebrafish Merkel cells. Merkel cells in zebrafish develop from basal keratinocytes and exhibit neurosecretory and mechanosensory machinery, actin-rich microvilli, and complex with somatosensory axons, similar to mammalian Merkel cells. The study also found region-specific densities and distribution patterns of Merkel cells in different skin compartments.
Article
Multidisciplinary Sciences
M. Fethullah Simsek, Ertugrul M. Ozbudak
Summary: The study presents a flattened 3-D tissue culture method for live imaging of zebrafish tail explants, allowing for a proportional slowdown of axis elongation and untangling the chemical input of signaling gradients from the mechanistic input of axial elongation. This method can provide new insights for future studies on vertebrate segmentation and potential pharmaceutical perturbations.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Cell Biology
Michka Sharpe, Juan Manuel Gonzalez-Rosa, Felicia Wranitz, Spencer Jeffrey, Katherine Copenhaver, C. Geoffrey Burns, Caroline E. Burns
Summary: Cardiomyocyte proliferation is an important source of new myocardium during heart development and regeneration, and Ruvbl2 functions as a suppressor of cardiomyocyte proliferation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Developmental Biology
Benoit Haerlingen, Robert Opitz, Isabelle Vandernoot, Angelo Molinaro, Meghna Parakkal Shankar, Pierre Gillotay, Achim Trubiroha, Sabine Costagliola
Summary: Thyroid tissue is derived from ventral pharyngeal endoderm and defects in morphogenesis are a main cause of congenital thyroid diseases. Recent studies have revealed the importance of FGF and BMP signaling in thyroid specification, but the relationship between signaling cues and thyroid transcription factors is still unclear. By analyzing zebrafish embryos, researchers identified a population of Pax2a-expressing thyroid progenitor cells that show enhanced FGF signaling but lack Nkx2.4b expression and BMP signaling. Manipulation of FGF/BMP activities suggests that FGF signaling mainly regulates Pax2a expression, while BMP signaling regulates both Pax2a and Nkx2.4b expression. Similar expression dynamics were observed in mouse embryos, indicating that this refined model of thyroid cell specification is evolutionarily conserved in mammals.
Article
Biochemistry & Molecular Biology
Zulvikar Syambani Ulhaq, Yukiko Ogino, William Ka Fai Tse
Summary: Using zebrafish as a model, this study demonstrates that the absence of functional trappc11 leads to limb-girdle muscular dystrophy R18, characterized by compromised skeletal muscle function, muscle disorganization, and myofibrosis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Cell Biology
Chen Zhou, Danying Chen, Jianhan Ren, Delan Huang, Runze Li, Haotian Luo, Chenyu Guan, Yang Cao, Weicai Wang
Summary: The co-expression pattern of Lhx8 and Suv39h1 in dental mesenchyme is regulated by epithelium-mesenchymal signals, influencing the differentiation and proliferation of dental mesenchyme. The Lhx8/Suv39h1 complex represses odontoblast differentiation-related genes through methylating H3K9 on their promoters.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Biology
Filomena Caccavale, Giovanni Annona, Lucie Subirana, Hector Escriva, Stephanie Bertrand, Salvatore D'Aniello
Summary: During amphioxus embryogenesis, the functional cooperation between NO and RA is crucial for normal pharyngeal development, with NO modulating RA production and RA regulating Nos gene transcription. This reciprocal regulation of NO and RA pathways may be conserved in vertebrates.
Article
Biology
Giacomo L. Glotzer, Pietro Tardivo, Elly M. Tanaka
Summary: The expression of fibroblast growth factors (Fgf) ligands in the Apical Ectodermal Ridge (AER) is a conserved feature of limb development in vertebrates. However, the axolotl, a salamander species, has a unique mesenchymal expression of key Fgf ligands in its developing and regenerating limb. This study investigates the hypothesis that alterations in transcription factors and Wnt signaling during evolution have resulted in this unique expression pattern in the axolotl. The results suggest that a shift in tissue responsiveness to canonical Wnt signaling from epidermis to mesenchyme is one contributing factor to the mesenchymal expression of Fgf ligands in the axolotl.
Article
Biochemical Research Methods
Joel Dokmegang, Hanh Nguyen, Elena Kardash, Thierry Savy, Matteo Cavaliere, Nadine Peyrieras, Rene Doursat
Summary: The study aims to gain insights into the development of vertebrate limbs by understanding the mechanisms of zebrafish pectoral fin development. Results show that zebrafish pectoral fin cells have a preferential elongation axis, and the axis of cell polarity is a crucial factor in driving fin morphogenesis.
Article
Biochemistry & Molecular Biology
Ayumi Miyake, Takatoshi Ohmori, Yuka Murakawa
Summary: Fgf22 plays a crucial role in the formation of the zebrafish forebrain by determining ventral properties and suppressing oligodendrocyte differentiation.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Cell Biology
Ramona Dries, Annemarie Lange, Sebastian Heiny, Katja I. Berghaus, Martin Bastmeyer, Joachim Bentrop
Summary: The study demonstrates the importance of Neural Cell Adhesion Molecule Ncam1b in the development of the pLLS in zebrafish, as it plays a role in activating proliferation by triggering the expression of erm. Additionally, Ncam1b is crucial for directing primordial migration and strengthening the cohesion of proneuromast cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Tanveer Teranikar, Cameron Villarreal, Nabid Salehin, Toluwani Ijaseun, Jessica Lim, Cynthia Dominguez, Vivian Nguyen, Hung Cao, Cheng-Jen Chuong, Juhyun Lee
Summary: Quantitative assessment of structural and functional biomarkers in vivo is crucial for understanding congenital disorders. This study demonstrates the potential of LSFM as a non-invasive tool for evaluating cardiac mechano-transduction in zebrafish.