Article
Biochemistry & Molecular Biology
Guenter A. Mueller, Timo D. Mueller
Summary: This study investigated the intercellular transfer of GPI-APs, which found that full-length GPI-APs can be transferred between cells and regulate metabolism by upregulating lipid and glycogen synthesis. The transfer and synthesis were inhibited by serum proteins and promoted by PIGs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Zhongwu Guo
Summary: GSL and GPI are two major glycolipids expressed by eukaryotic cells, and their metabolisms are similar. Furthermore, GSLs and GPI-APs are localized in lipid rafts, which are cholesterol-rich regions of the cell membrane where many signaling molecules are compartmentalized. Thus, the interactions between GSLs, GPI-APs, and other molecules in lipid rafts are inevitable.
Article
Biochemical Research Methods
Yang Zhou, Fei Zhao, Bo Zheng, Shihai Tang, Juan Gong, Bin He, Zhi Zhang, Na Jiang, Huijuan Zha, Jun Luo
Summary: This study develops a new electrochemical method for the feasible detection of cardiomyocyte-derived extracellular vesicles (EVs) in biological fluids. The method employs a peptide-anchored biomimetic interface to achieve efficient and selective detection of target EVs. The results demonstrate a wide linear detection range and good performance in biological fluids.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Yi-Shi Liu, Emmanuel Matabaro, Xiao-Dong Gao, Morihisa Fujita
Summary: The study established a GPS system using GPI-anchored proteins to select cells producing high levels of target secretory proteins, providing a promising method to improve recombinant protein production. It successfully eliminated GPI-anchored proteins by knocking out genes, resulting in secretion of all proteins.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2021)
Article
Plant Sciences
Cesar Bernat-Silvestre, Yingxuan Ma, Kim Johnson, Alejandro Ferrando, Fernando Aniento, Maria Jesus Marcote
Summary: This study investigates the function of Arabidopsis Per1/PGAP3 genes, and finds that PGAP3A is essential for the efficient transport of GPI-anchored proteins from the ER to the plasma membrane/cell wall. The loss of PGAP3A function increases susceptibility to salt and osmotic stresses, possibly due to altered localization of GPI-APs. In addition, PGAP3B is shown to be a functional ortholog of Per1p.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Microbiology
Relber A. Goncales, Ayda L. M. Salamanca, Luiz R. B. Junior, Kleber S. F. Silva, Elton J. R. de Vasconcelos, Thaila F. dos Reis, Ricardo C. Castro, Patricia de C. Ruy, Barbara Romagnoli, Jeronimo Ruiz, Maristela Pereira, Celia M. Soares, Paulo S. R. Coelho
Summary: The aim of this study was to predict GPI-anchored proteins in Paracoccidioides brasiliensis and Paracoccidioides lutzii genomes using bioinformatics tools and proteomic analysis. A total of 100 GPI-anchored proteins were predicted, with 57 classified in functional categories and 43 conserved proteins with unknown functions. Four proteins identified by in silico analyses were also found in the cell wall proteome. These findings provide important resources for future research on GPI-anchored proteins in Paracoccidioides spp. to develop new diagnostic tools, drugs, and immunological tests.
FUTURE MICROBIOLOGY
(2021)
Article
Plant Sciences
Cesar Bernat-Silvestre, Judit Sanchez-Simarro, Yingxuan Ma, Javier Montero-Pau, Kim Johnson, Fernando Aniento, Maria Jesus Marcote
Summary: Glycosylphosphatidylinositol (GPI)-anchored proteins are crucial for various plant processes, and lipid remodeling is essential for their export to the cell surface. Arabidopsis PGAP1 acts as a GPI inositol-deacylase, facilitating the efficient ER export and transport of GPI-APs.
Article
Biochemistry & Molecular Biology
Ahmed Hammad Mirza, Liujuan Cui, Shuyan Zhang, Pingsheng Liu
Summary: Brown adipose tissue (BAT) contains lipid droplet-anchored mitochondria (LDAM) which are functional across different temperatures, with comparative proteomics analysis revealing differential protein expressions between LDAM and cytoplasmic mitochondria (CM) at different temperatures, indicating a preorganized and functional organelle complex.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Article
Biochemistry & Molecular Biology
Shu-Ya Cao, Yi-Shi Liu, Xiao-Dong Gao, Taroh Kinoshita, Morihisa Fujita
Summary: Protein modification by glycosylphosphatidylinositol (GPI) occurs in the endoplasmic reticulum (ER) and GPI-anchored proteins (GPI-APs) are transported from the ER to the cell surface through the Golgi apparatus. The loss of TMEM41B, an ER-localized lipid scramblase, restores the sensitivity of GPI-APs to bacterial phospholipase C (PI-PLC) and delays the transport of GPI-APs and transmembrane proteins. Additionally, the turnover of PGAP1, mediated by ER-associated degradation, is slowed in TMEM41B-KO cells.
JOURNAL OF BIOCHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Guenter A. Mueller, Timo D. Mueller
Summary: Glycosylphosphatidylinositol-anchored proteins (GPI-APs) are anchored to the outer leaflet of plasma membranes through covalent linkage to a highly conserved glycolipid. They can be released into the surrounding environment, undergoing distinct arrangements upon loss of their GPI anchor or incorporation into various complexes. In mammals, the (patho)physiological roles of released GPI-APs depend on their release mechanisms, cell types, and tissues involved, and are controlled by their removal from circulation.
Article
Biochemistry & Molecular Biology
Marianne Zaruba, Lena Roschitz, Haider Sami, Manfred Ogris, Wilhelm Gerner, Christoph Metzner
Summary: Extracellular vesicles produced by cells, including bacteria, can be functionalized for various applications, such as gene therapy, vaccine production and diagnostics. By modifying outer membrane vesicles of E. coli with GPI-anchored proteins, eukaryotic proteins can be displayed on a prokaryotic platform, potentially creating a versatile modular platform for novel vaccine strategies.
Article
Endocrinology & Metabolism
Arthe Raajendiran, Christoph Krisp, David P. De Souza, Geraldine Ooi, Paul R. Burton, Renea A. Taylor, Mark P. Molloy, Matthew J. Watt
Summary: Adipose tissue plays a crucial role in energy balance and metabolism regulation, with differences in protein composition leading to metabolic variation in different regions. Upper-body adipocytes are associated with glycolysis, lipogenesis, and oxidative stress, while lower-body adipocytes show enhanced activation of PPARa and mitochondrial oxidative phosphorylation processes.
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2021)
Article
Biotechnology & Applied Microbiology
Magnus Halldor Gislason, Henrik Nielsen, Jose Juan Almagro Armenteros, Alexander Rosenberg Johansen
Summary: GPI anchors are crucial for linking proteins to the outer face of the plasma membrane in eukaryotic cells. Researchers have developed a new method, NetGPI, based on recurrent neural networks and attention mechanism, for predicting GPI anchoring signals. NetGPI outperforms existing methods in discriminating between GPI-anchored proteins and other secretory proteins.
CURRENT RESEARCH IN BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Gunter A. Mueller, Andreas Lechner, Matthias H. Tschoep, Timo D. Mueller
Summary: The release of GPI-APs into the blood in response to metabolic stress and aging is influenced by interaction with serum proteins like GPLD1, affecting cell surface integrity. Upregulation of GPLD1 interaction with GPI-AP complexes is associated with hyperglycemic/hyperinsulinemic conditions. Serum proteins could modulate the translocation and lysis of GPI-APs.
Review
Immunology
Jeu Park, Jee Hyung Sohn, Sang Mun Han, Yoon Jeong Park, Jin Young Huh, Sung Sik Choe, Jae Bum Kim
Summary: Adipose tissue is an immunologically active organ that interacts with various immune cells, with lipid metabolites playing a key role in determining adipose immune responses. The interactions between adipocytes and immune cells in adipose tissue control adipocyte fate and functions, particularly in response to metabolic stimuli. Additionally, adipose stem cells have been found to play a role in regulating adipose tissue immunity.
FRONTIERS IN IMMUNOLOGY
(2021)
