Article
Pharmacology & Pharmacy
Jorid B. Sorlia, Alexander C. O. Jensen, Alicja Mortensen, Jozef Szarek, Claudia A. T. Gutierrez, Lucas Givelet, Katrin Loeschner, Charis Loizides, Iosif Hafez, George Biskos, Ulla Vogel, Niels Hadrup
Summary: By conducting nose-only inhalation tests on mice, we found that the air concentration of molybdenum disulphide particles has no observed adverse effect on body weight gain and genotoxicity below 13mg/m³, while the effects on respiratory function suggest this level as the lowest observed adverse effect concentration.
Article
Multidisciplinary Sciences
Mirko Marino, Letizia Gigliotti, Peter Moller, Patrizia Riso, Marisa Porrini, Cristian Del Bo
Summary: The comet assay is used to assess DNA damage in tissues and biological samples. This study found that storage time had varying effects on DNA damage levels in PBMCs and WB samples, with significant increases observed in WB samples stored for over 6 months. WB samples showed higher levels of DNA damage compared to PBMCs.
SCIENTIFIC REPORTS
(2021)
Article
Public, Environmental & Occupational Health
Martin Roursgaard, Monika Hezareh Rothmann, Juliane Schulte, Ioanna Karadimou, Elena Marinelli, Peter Moller
Summary: This study investigated the extraction of nanoplastic particles from food containers and found that these nanoparticles can cause genotoxicity in cell cultures, while their cytotoxic effects were limited.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Food Science & Technology
Merve Bacanli, Ozgur Esim, Hakan Erdogan, Meral Sarper, Onur Erdem, Yalcin Ozkan
Summary: This study evaluated the cytotoxicity and genotoxicity of paclitaxel nanoencapsulated with PLGA in human neuroblastoma cells. Results showed that PLGA nanoparticles can be considered as a biocompatible carrier system for drug delivery and might be a promising agent against neuroblastoma.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Article
Biology
Taiki Sato, Kazuomi Sato
Summary: This study evaluated the impact of 5-aminolevulinic acid (5-ALA) and blue light on DNA damage. The results showed that blue light irradiation severely damaged the genomic DNA of both cancer cells and non-cancer cells in the presence of 5-ALA.
Article
Pharmacology & Pharmacy
Atinuke F. Ojo, Cheng Peng, Jack C. Ng
Summary: This study evaluates the genotoxicity of PFAS mixtures in a human cell line system. The results show that PFAS and their mixtures cause a concentration-dependent decrease in cell viability and a modest increase in cellular DNA damage under these conditions.
Article
Toxicology
Ezgi Eyluel Bankoglu, Franzisca Stipp, Johanna Gerber, Florian Seyfried, August Heidland, Udo Bahner, Helga Stopper
Summary: The comet assay is a widely used method for assessing DNA damage and repair activity in various samples, with cryopreserved blood preparations found to be suitable for such analysis. The study also revealed that ex vivo hydrogen peroxide challenge had optimal effects in PBMCs, while repair comet assays worked well in fresh PBMCs.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Toxicology
Ji-Eun Seo, Xiaoqing Guo, Dayton M. Petibone, Sharon D. Shelton, Ying Chen, Xilin Li, Volodymyr Tryndyak, Stephanie L. Smith-Roe, Kristine L. Witt, Nan Mei, Mugimane G. Manjanatha
Summary: The study confirmed the induction of micronuclei by black cohosh extract in TK6 and HepG2 cells, as well as DNA damage in TK6 cells. Exposure to 125 µg/ml BCE (24 h) led to G1/S arrest in TK6 cells, accompanied by apoptosis and increased expression of related proteins.
TOXICOLOGICAL SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Arshad H. Mir, Vinod K. Dumka, Faheem Sultan, Milindmitra K. Lonare
Summary: The components of oral contraceptive pills, drospirenone and ethinylestradiol, cause DNA damage in breast cells and bone marrow cells. This damage may lead to cancer development in women who take these drugs for a long period of time.
DRUG AND CHEMICAL TOXICOLOGY
(2022)
Review
Environmental Sciences
Aryelle Canedo, Thiago Lopes Rocha
Summary: Zebrafish have become a popular model for assessing the genotoxicity of pollutants due to their orthologous genes and involvement in repair pathways. Comet assay is the main technique used in these studies, but standardization is needed for better interpretation and comparisons. Understanding repair mechanisms in zebrafish subjected to DNA damage is essential for further research on genotoxic effects caused by pollutants.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Environmental Sciences
Marcelino Benvindo-Souza, Daiany Folador Sotero, Cirley Gomes Araujo dos Santos, Rhayane Alves de Assis, Rinneu Elias Borges, Lia Raquel de Souza Santos, Daniela de Melo e Silva
Summary: Pollution from mining activities can cause genotoxic damage to bats, particularly in species like Carollia perspicillata and Glossophaga soricina. High levels of elements such as Mn, Cr, Pb, and Zn were found in water samples from the mining area. The study suggests that bats in mining areas are susceptible to increased DNA damage, similar to what has been observed in other species.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Environmental Sciences
Felipe de Araujo Nascimento, Daniela de Melo e Silva, Thays Millena Alves Pedroso, Jheneffer Sonara Aguiar Ramos, Michelle Rocha Parise
Summary: Occupational exposure to pesticides in farmers results in significant DNA damage, showing a notable impact and variability in the studies. Smokers who are occupationally exposed to pesticides have slightly reduced DNA damage compared to non-smokers, indicating some genetic toxicity. The study demonstrates a higher risk of occupationally exposed rural workers developing related diseases due to the genotoxic effect of pesticides.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Toxicology
Kevin Hogeveen, Sylvie Huet, Charlene Besnard, J. Sam Murray, D. Tim Harwood, Andy Selwood, Valerie Fessard
Summary: Portimine, a recently identified cyclic imine produced by the dinoflagellate Vulcanodinium rugosum, has been described as a potent apoptotic agent and may potentially induce DNA replication stress. Experimental results show that in HepaRG cells, Portimine induced phosphorylation of H2AX, but had no effects on other DNA damage endpoints.
TOXICOLOGY IN VITRO
(2021)
Article
Environmental Sciences
Vehbi Atahan Togay, Gulcin Yavuz Turel, Dilek Asci celik, Meltem ozgocmen, Eda Evgen Tuluceoglu, Ismail Sen, Yusuf Ayvaz
Summary: This study found that cyprodinil and thiacloprid exposure can lead to increased DNA damage risk in zebrafish, as demonstrated by comet assay. These pesticides were identified as genotoxic agents that require further investigation.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Irma Virant-Klun, Senka Imamovic-Kumalic, Bojana Pinter
Summary: Exposure to endocrine-disrupting chemicals can lead to oxidative stress and disruption of the endocrine system, affecting human reproduction and development. This review examines the association between endocrine disruptors and oxidative stress-related male infertility. It finds that bisphenols and phthalates have detrimental effects on semen quality and sperm DNA integrity.
Article
Toxicology
Hanna Katarina Lilith Johansson, Terje Svingen, Julie Boberg, Paul A. Fowler, David Stead, Anne Marie Vinggaard, Panagiotis Filis
ARCHIVES OF TOXICOLOGY
(2020)
Article
Chemistry, Applied
Lene Duedahl-Olesen, Tommy L. Cederberg, Tue Christensen, Sisse Fagt, Arvid Fromberg, Kit Granby, Max Hansen, Julie Boberg, Jens J. Sloth, Annette Petersen
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2020)
Article
Biochemistry & Molecular Biology
Majorie B. M. van Duursen, Julie Boberg, Sofie Christiansen, Lisa Connolly, Pauliina Damdimopoulou, Panagiotis Filis, Paul A. Fowler, Bart M. Gadella, Jan Holte, Kersti Jaager, Hanna K. L. Johansson, Tianyi Li, Severine Mazaud-Guittot, Anne-Simone Parent, Andres Salumets, Ana M. Soto, Terje Svingen, Agne Velthut-Meikas, Eva Bay Wedebye, Yuling Xie, Martin van den Berg
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Review
Medicine, Legal
Niels Hadrup, Anoop K. Sharma, Katrin Loeschner, Nicklas R. Jacobsen
REGULATORY TOXICOLOGY AND PHARMACOLOGY
(2020)
Review
Medicine, Legal
Niels Hadrup, Marie Frederiksen, Anoop K. Sharma
Summary: Boron is widely used as a flame retardant in insulation products, and high exposure may lead to unwanted health effects. It is mainly absorbed through the gastrointestinal tract after oral exposure, with intact skin serving as a barrier. Boron is primarily excreted via urine, and skin exposure can be fatal. Data on inhalation toxicity is limited, but skin exposure to boric acid has proven fatal in some cases.
REGULATORY TOXICOLOGY AND PHARMACOLOGY
(2021)
Article
Environmental Sciences
Monica Kam Draskau, Aurelie Lardenois, Bertrand Evrard, Julie Boberg, Frederic Chalmel, Terje Svingen
Summary: Research has shown that azole fungicides triticonazole and flusilazole can disrupt male reproductive development by different mechanisms, potentially causing feminization effects such as short AGD in fetal rats, which may be due to disturbed androgen signaling or direct targeting of steroidogenic enzyme activity in the testis.
Article
Reproductive Biology
Hanna K. L. Johansson, Sofie Christiansen, Monica Kam Draskau, Terje Svingen, Julie Boberg
Summary: Developmental exposure to endocrine disrupting chemicals can have negative effects on reproductive health in rats, with males being more affected than females. More sensitive test methods are needed to protect the reproductive health of females from harmful chemicals.
REPRODUCTIVE TOXICOLOGY
(2021)
Article
Pharmacology & Pharmacy
Monica Kam Draskau, Anna Kjerstine Rosenmai, Martin Scholze, Mikael Pedersen, Julie Boberg, Sofie Christiansen, Terje Svingen
Summary: Clotrimazole is a widely used antifungal drug, but studies have shown that its use during pregnancy can significantly alter hormone levels, potentially affecting the reproductive system of the fetus.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2021)
Review
Chemistry, Multidisciplinary
Niels Hadrup, Anoop K. Sharma, Nicklas R. Jacobsen, Katrin Loeschner
Summary: Some implantable medical devices contain silver, which can be released into surrounding tissues and bodily fluids. Studies show that silver can distribute to blood and other tissues, potentially passing the blood-brain barrier. Deposited as nano-sized particles, implanted silver can cause local reactions, including argyria.
DRUG AND CHEMICAL TOXICOLOGY
(2022)
Article
Food Science & Technology
Julie Boberg, Lea Bredsdorff, Annette Petersen, Nathalie Lobl, Bodil Hamborg Jensen, Anne Marie Vinggaard, Elsa Nielsen
Summary: Researchers have developed fit-for-purpose criteria for grouping of chemicals and a web-based tool for mixture risk assessment, which includes hazard and exposure estimates for over 200 chemicals in foods and environment. The tool can be used to assess mixture risks and to identify main drivers of risk, emphasizing the importance of solid scientific insight for result interpretation.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Article
Pharmacology & Pharmacy
Julie Boberg, Hanna K. L. Johansson, Delphine Franssen, Johannah H. Cramer, Diana Usai, Mikael Pedersen, Anne-Simone Parent, Terje Svingen
Summary: This study examined the effects of perinatal exposure to anti-androgens DEHP and procymidone on ovarian development in female rat offspring. The results showed an increase in newly recruited follicles and altered steroid hormone levels in prepubertal animals, as well as a trend towards accelerated loss of follicle reserves in adulthood. These findings suggest that exposure to these chemicals may have severe consequences for female fertility.
Review
Pharmacology & Pharmacy
Niels Hadrup, Jorid B. Sorli, Anoop K. Sharma
Summary: Molybdenum, lithium, and tungsten are constituents of many products and exposure to these elements potentially occurs at work. Dose descriptors for inhalation toxicity were identified and the potential for genotoxicity and carcinogenicity was assessed.
Article
Environmental Sciences
Yvette Dirven, Dag Markus Eide, Erika Witasp Henriksson, Rune Hjorth, Anoop Kumar Sharma, Anne Graupner, Gunnar Brunborg, Jarle Ballangby, Anne Mette Zenner Boisen, Stellan Swedmark, Kristine Bjerve Gutzkow, Ann-Karin Olsen
Summary: The in vivo comet assay is commonly used to measure genotoxicity, but it is not recommended for assessing testicular germ cells according to the current OECD test guideline (TG 489) due to the presence of somatic cells. This study provides a proof-of-concept to selectively analyze different testicular cell populations using the comet assay, enabling the assessment of genotoxicity in germ cells. The developed framework allows for the identification of round spermatids and primary spermatocytes based on differences in DNA content and appearance.
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS
(2023)
Meeting Abstract
Environmental Sciences
Y. Dirven, D. M. Eide, A. Sharma, K. B. Gutzkow, Ann Karin Olsen
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS
(2022)
Article
Toxicology
Marianne Dybdahl, David Benjamin Selesko, Ulla Ramer Mikkelsen
Summary: The safety of Lacprodan (R) BLG was evaluated for genotoxicity and subchronic toxicity, showing no mutagenic potential in bacterial reverse mutation assay or clastogenic or aneugenic potential in an in vitro micronucleus assay. In a 90-day subchronic toxicity study on Wistar rats, no treatment-related adverse effects were observed at dose levels up to 1000 mg/kg bw/day, establishing the NOAEL for Lacprodan (R) BLG.
TOXICOLOGY REPORTS
(2021)
