Article
Veterinary Sciences
Zebang Xu, Xinxin Xu, Bin Yang, Yuling Mi, Jiakun Wang
Summary: Research has shown that it is feasible to establish organoids from single rumen epithelial cells, which is a novel in vitro system that may reduce the use of experimental animals and can be used to study host and pathogen interaction.
VETERINARY RESEARCH
(2023)
Article
Reproductive Biology
Junyuan Ma, Jine Wang, Songming Hu, Yang Li, Yaxin Zhang, Yahua Yang, Chongfa Yang, Shengdong Huo, Yanmei Yang, Yingpai Zhaxi, Wenxue Luo
Summary: Theca cells (TCs) in the ovary have a unique role in follicle structure and function, affecting follicle development and atresia. The study found that sheep TCs express melatonin receptors (MT1 and MT2). Different concentrations of melatonin (MT) were investigated for their effects on antioxidant levels, proliferation, apoptosis, and steroid hormone secretion in sheep TCs. The results showed that MT inhibited apoptosis and proliferation, increased progesterone secretion, and activated the PI3K/Akt pathway in TCs. These findings contribute to understanding the role of TCs in follicle development and ovarian hormone secretion.
Article
Reproductive Biology
Xiaodan Li, Xiaolei Yao, Haiqiang Xie, Mingtian Deng, Xiaoxiao Gao, Kaiping Deng, Yongjin Bao, Qi Wang, Feng Wang
Summary: This study revealed that SPATA6 is highly expressed in the testis of Hu sheep and localized in LCs and spermatogenic cells. SPATA6 plays a key role in regulating the proliferation, apoptosis, and testosterone biosynthesis of LCs, contributing to sheep spermatogenesis.
Article
Biology
Lauren E. Carter, David P. Cook, Curtis W. McCloskey, Melanie A. Grondin, David A. Landry, Tiffany Dang, Olga Collins, Lisa F. Gamwell, Holly A. Dempster, Barbara C. Vanderhyden
Summary: The authors used spheroids as a model to study the molecular signatures of ovarian surface epithelial cells exhibiting stemness phenotype in vitro, finding a high degree of heterogeneity in the transcriptional profiles of stem cells induced under different conditions. They suggest that maintenance of the ovarian surface epithelium may not require a single stem cell population but instead rely on heterogeneous stem cells induced transiently under diverse environmental cues, indicating a more complex and dynamic system in regulating the stem cell characteristics in this tissue.
COMMUNICATIONS BIOLOGY
(2021)
Review
Endocrinology & Metabolism
Sabrina J. Ritch, Carlos M. Telleria
Summary: Epithelial ovarian cancer is the deadliest gynecological disease, commonly diagnosed at late stages with metastasis. Understanding the unique microenvironments that promote EOC dissemination is crucial in improving patient outcomes.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Environmental Sciences
Alexander V. Sirotkin, Martina Macejkova, Adam Tarko, Zuzana Fabova, Saleh Alwasel, Abdel Halim Harrath
Summary: The study found that xylene suppresses ovarian cell functions, but buckwheat, rooibos, and vitex can mitigate these effects, acting as natural protectors against the adverse effects of xylene on ovarian cells.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Multidisciplinary Sciences
Menghe Liu, Katja Hummitzsch, Nicole A. Bastian, Monica D. Hartanti, Qianhui Wan, Helen F. Irving-Rodgers, Richard A. Anderson, Raymond J. Rodgers
Summary: During ovarian development, GREL cells and ovarian fibroblasts exhibit distinct gene expression profiles, which also differ from the cells they ultimately differentiate into.
Article
Multidisciplinary Sciences
Menghe Liu, Katja Hummitzsch, Nicole A. Bastian, Monica D. Hartanti, Qianhui Wan, Helen F. Irving-Rodgers, Richard A. Anderson, Raymond J. Rodgers
Summary: During ovarian development, GREL cells and fetal fibroblasts exhibit distinct morphological and gene expression profiles. Compared to fetal fibroblasts, GREL cells show higher expression of some genes and lower expression of others.
Article
Biochemistry & Molecular Biology
Juntae Kwon, Hyeongjwa Choi, Anna D. Ware, Bernadette Cecilia Morillo, Haiyang Wang, Kerrie B. Bouker, Xiongbin Lu, Todd Waldman, Cecil Han
Summary: Epithelial ovarian cancer is a lethal gynecologic malignancy, with USP13 gene amplification and overexpression being correlated with poor survival outcomes. In a mouse model, overexpression of USP13 accelerated ovarian tumorigenesis and led to decreased survival, enhanced peritoneal metastasis, and development of hemorrhagic ascites. Inhibition of USP13 decreased the viability of ovarian cancer cells and reduced tumorigenic and metastatic abilities. USP13 may be a potential therapeutic target for ovarian cancer.
Article
Medicine, Research & Experimental
Wyatt E. Lanik, Cliff J. Luke, Lila S. Nolan, Qingqing Gong, Lauren C. Frazer, Jamie M. Rimer, Sarah E. Gale, Raymond Luc, Shay S. Bidani, Carrie A. Sibbald, Angela N. Lewis, Belgacem Mihi, Pranjal Agrawal, Martin Goree, Marlie Maestas, Elise Hu, David G. Peters, Misty Good
Summary: Necrotizing enterocolitis (NEC) is a deadly gastrointestinal disease in premature infants. An in vitro model called Neonatal-Intestine-on-a-Chip was developed to mimic the key features of intestinal physiology in premature infants. This model named NEC-on-a-Chip, utilizing infant-derived microbiota, successfully replicated the pathophysiology of NEC and provides a valuable tool for studying the disease and testing new therapies.
Article
Biochemistry & Molecular Biology
Marie-Christin Beer, Heidrun Kuhrt, Leon Kohen, Peter Wiedemann, Andreas Bringmann, Margrit Hollborn
Summary: Dedifferentiation and proliferation of RPE cells are characteristics of retinal diseases. Kir4.2 channels play functional roles in maintaining the viability and proliferation of RPE cells. Upregulation of Kir4.2 mRNA occurs under hypoxia and extracellular hyperosmolarity conditions, while downregulation is induced by VEGF, blood serum, and thrombin. Silencing of Kir4.2 leads to decreased cell viability and proliferation.
Article
Agriculture, Dairy & Animal Science
Mingliang He, Weibo Zhang, Shan Wang, Ling Ge, Xiukai Cao, Shanhe Wang, Zehu Yuan, Xiaoyang Lv, Tesfaye Getachew, Joram M. Mwacharo, Aynalem Haile, Wei Sun
Summary: This study aimed to investigate the expression level of YAP1 in the skeletal muscle of Hu sheep and its regulatory mechanism on skeletal muscle satellite cells (SMSCs). The results showed that YAP1 was highly expressed in the skeletal muscle of Hu sheep, and its overexpression promoted SMSCs proliferation and inhibited differentiation, while interference of YAP1 had the opposite effect. Therefore, YAP1 is an important functional molecule in the growth and development of skeletal muscle.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Biology
Jingqiang Wang, Chunye Liu, Lingli He, Zhiyao Xie, Lanyue Bai, Wentao Yu, Zuoyun Wang, Yi Lu, Dong Gao, Junfen Fu, Lei Zhang, Yi Arial Zeng
Summary: The activation and proliferation of ovarian surface epithelium stem cells after ovulatory rupture is triggered by selective activation of YAP signaling and lower expression of Vgll4 in Procr progenitors. YAP activation promotes Procr transcription and the combination of increased cell division and Procr expression leads to expansion of Procr+ progenitors surrounding the rupture site.
Article
Agriculture, Dairy & Animal Science
Mingliang He, Hui Zhou, Tingyan Hu, Xiaoyang Lv, Shanhe Wang, Xiukai Cao, Zehu Yuan, Kai Quan, Tesfaye Getachew, Joram M. Mwacharo, Aynalem Haile, Wei Sun
Summary: This study aimed to investigate the role of melatonin in dermal papilla cells. Experimental results showed that treatment with 1000 pg/ml melatonin promoted cell proliferation, inhibited apoptosis, and enhanced cell cycle progress in dermal papilla cells.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Reproductive Biology
I. P. Silva, D. C. C. Brito, T. E. S. Silva, R. F. Silva, M. I. F. Guedes, J. Y. G. Silva, A. P. R. Rodrigues, R. R. Santos, J. R. Figueiredo
Summary: This study evaluated the effects of ZEN and ENL, alone or in combination, on the survival and morphology of in vitro cultured ovarian preantral follicles. The results showed that while ZEN impaired the morphology of follicles, ENL improved the cultured secondary follicles. However, the combination of ENL with ZEN impaired the quality of primary and secondary follicles.