Article
Biochemistry & Molecular Biology
Roberta Genova, Pablo Laborda, Trinidad Cuesta, Jose Luis Martinez, Fernando Sanz-Garcia
Summary: Understanding the consequences of drug resistance in bacterial physiology is crucial for exploiting its weaknesses. Collateral sensitivity, a potentially exploitable phenotype, may not be conserved among different isolates. Identifying robust and conserved collateral sensitivity patterns is important for translating this knowledge into clinical practice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Chelsey M. VanDrisse, Rosalie Lipsh-Sokolik, Olga Khersonsky, Sarel J. Fleishman, Dianne K. Newman
Summary: This study demonstrates that by engineering the structure of PodA protein and combining it with tobramycin, the killing effect on P. aeruginosa can be enhanced, especially on biofilms that are resistant to conventional antibiotic treatment.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Microbiology
Telma de Sousa, Michel Hebraud, Olimpia Alves, Eliana Costa, Luis Maltez, Jose Eduardo Pereira, Angela Martins, Gilberto Igrejas, Patricia Poeta
Summary: A study conducted at the Medical Center of Tras-os-Montes and Alto Douro (CHTMAD) found that Pseudomonas aeruginosa strains causing urinary tract infections are resistant to multiple antibiotics and have high biofilm formation and motility capabilities, posing a serious public health problem.
Article
Chemistry, Multidisciplinary
Carly J. Carter, Krishna Pillai, Samina Badar, Ahmed H. Mekkawy, Javed Akhter, Thomas Jefferies, Sarah J. Valle, David L. Morris
Summary: The study found that the combination of Bromelain and NAC, known as BromAc, had good dissolution efficacy rates of 80% and above on biofilms produced by three strains of Pseudomonas aeruginosa. The combination exhibited significant impact on all three strains, with Bromelain degrading biofilms more effectively while NAC showed growth enhancement in two strains. The results suggest that BromAc may be a promising treatment option for eradicating biofilms formed by Pseudomonas aeruginosa strains.
APPLIED SCIENCES-BASEL
(2021)
Article
Pharmacology & Pharmacy
Nuoyan Wang, Xuemin Chen, Jing Luo, Fei Deng, Fuguo Shi, Qin Wu, Yasi Huang, Qin Ouyang, Rongxin Qin, Hong Zhou
Summary: This study investigated the antibacterial sensitization effect of DHA27 combined with tobramycin in tobramycin-resistant Pseudomonas aeruginosa. The combination showed significant antibacterial effects on many resistant strains and reduced bacterial load in the spleen and lungs in a mouse model. The study also revealed that the effect was related to the inhibition of mRNA expression of aminoglycoside-modifying enzymes.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Microbiology
Theerthankar Das, Biswanath Das, Brandon Clark Young, Vina Aldilla, Shekh Sabir, Basmah Almohaywi, Mark Willcox, Mike Manefield, Naresh Kumar
Summary: The presence of ascorbic acid can interact with pyocyanin, resulting in shifts in its absorbance peaks and structure. This interaction impairs the binding of pyocyanin to DNA and disrupts biofilm formation. In combination with furanone-30, ascorbic acid further inhibits quorum sensing and enhances antibiotic-mediated bacterial killing, reducing the virulence and biofilm formation of Pseudomonas aeruginosa. The antioxidant properties of ascorbic acid play a crucial role in these interactions and their effects.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Microbiology
Rui Yin, Juanli Cheng, Jingyao Wang, Panxin Li, Jinshui Lin
Summary: Pseudomonas aeruginosa, a major pathogen in human infections, forms biofilms due to the secretion of extracellular polymeric substances, leading to antibiotic resistance. New treatment technologies, such as antimicrobial peptides and bacteriophage therapy, have been developed to target P. aeruginosa biofilms.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Instruments & Instrumentation
Delia Boffoli, Federica Bellato, Greta Avancini, Pratik Gurnani, Gokhan Yilmaz, Manuel Romero, Shaun Robertson, Francesca Moret, Federica Sandrelli, Paolo Caliceti, Stefano Salmaso, Miguel Camara, Giuseppe Mantovani, Francesca Mastrotto
Summary: Carbohydrate-based materials, particularly synthetic glycopolymers, are attractive for their low toxicity and immunogenicity, and can be used as multivalent ligands to target sugar-binding proteins. This study utilized RAFT polymerisation to synthesize novel diblock copolymers capable of complexing aminoglycoside antibiotic tobramycin for targeted drug delivery against Pseudomonas aeruginosa biofilms. The tobramycin-loaded complexes efficiently suppressed in vitro biofilm formation, highlighting their potential in combating nosocomial infections.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2022)
Article
Microbiology
So-Young Ham, Han-Shin Kim, Min Jee Jo, Jeong-Hoon Lee, Youngjoo Byun, Gang-Jee Ko, Hee-Deung Park
Summary: The combination of 6-gingerol analog and tobramycin showed stronger inhibitory effects on biofilm formation and virulence factor production compared to individual treatments in P. aeruginosa infections. This combined treatment reduced infectivity without inducing cytotoxic effects in human lung epithelial cells. The adjuvant effects of this combination may lead to improved therapeutic development in chronic infections.
MICROBIOLOGY SPECTRUM
(2021)
Review
Microbiology
Dina Zheng, Phillip J. Bergen, Cornelia B. Landersdorfer, Elizabeth B. Hirsch
Summary: Multidrug-resistant Pseudomonas aeruginosa is a serious public health threat, and fosfomycin may be a therapeutic alternative. However, the lack of susceptibility breakpoints for fosfomycin and various resistance mechanisms limit its clinical use and efficacy.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Fereshteh Jabalameli, Mohammad Emaneini, Reza Beigverdi, Shahnaz Halimi, Maryam Siroosi
Summary: This study investigated the effects of nitrate, arginine, and ferrous on antibiotic recalcitrance in Pseudomonas aeruginosa biofilms. The results showed that these supplements reduced the number of viable cells, with ferrous significantly increasing biofilm susceptibility to antibiotics in all strains. Additionally, treating a strain with both ferrous and amikacin significantly decreased the expression of the mexY gene, which is involved in antibiotic resistance.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2023)
Article
Microbiology
Florence Chijindu Ugwuanyi, Abraham Ajayi, David Ajiboye Ojo, Adeyemi Isaac Adeleye, Stella Ifeanyi Smith
Summary: The study found that Pseudomonas aeruginosa strains isolated from clinical samples in Abeokuta Ogun state Nigeria exhibited multidrug resistance, biofilm formation, and efflux pump capabilities. Various relationships between biofilm formation and resistance to different antibiotics were observed.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2021)
Article
Infectious Diseases
Paul A. Akinduti, Onome W. George, Hannah U. Ohore, Olusegun E. Ariyo, Samuel T. Popoola, Adenike I. Adeleye, Kazeem S. Akinwande, Jacob O. Popoola, Solomon O. Rotimi, Fredrick O. Olufemi, Conrad A. Omonhinmin, Grace I. Olasehinde
Summary: This study explores the role of biofilm and efflux pump activities in high-level resistance of Pseudomonas aeruginosa strains in clinical infection. It finds that biofilm formers and strains with high efflux pump activity are associated with multidrug resistance. Furthermore, the study identifies specific risk factors for MDR-P. aeruginosa infection. The findings highlight the importance of addressing biofilm formation and efflux pump activity for clinical management and infection control.
Article
Infectious Diseases
Karan Gandhi, Shiv Dhiman, Rajat Arora, Danzel Marie Ramirez, Danyel Ramirez, Gilbert Arthur, Frank Schweizer
Summary: Metal ions, including Fe3+, influence the binding of antibiotics and the uptake of antibiotics by bacteria. Amphiphilic tobramycins, a new class of antibiotic potentiators, synergize with various antibiotics against Gram-negative bacteria. In this study, iron-deferoxamine conjugates were prepared and tested for their antibacterial activity and synergistic effects with antibiotics. The conjugates showed strong synergistic effects with outer-membrane-impermeable antibiotics and selectively improved the susceptibility of multidrug-resistant bacteria to tetracyclines. However, the antibiotic potentiating effect was not influenced by the Fe3+ concentration.
Article
Biochemical Research Methods
Salome Sauvage, Charlotte Gaviard, Ali Tahrioui, Laurent Coquet, Hung Le, Stephane Alexandre, Ahmed Ben Abdelkrim, Emeline Bouffartigues, Olivier Lesouhaitier, Sylvie Chevalier, Thierry Jouenne, Julie Hardouin
Summary: In this study, the impact of various carbon source supplementations on the physiology of the Pseudomonas aeruginosa PA14 strain was investigated using a global proteomic approach and phenotypic assays. The results showed that citrate supplementation enhanced the virulence, motility, biofilm development, and antibiotic resistance of the strain.
JOURNAL OF PROTEOME RESEARCH
(2022)