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Biochemistry & Molecular Biology
Jamin B. Hein, Hieu T. Nguyen, Dimitriya H. Garvanska, Isha Nasa, Thomas Kruse, Yinnian Feng, Blanca Lopez Mendez, Norman Davey, Arminja N. Kettenbach, Polly M. Fordyce, Jakob Nilsson
Summary: Investigating the specificity of phosphoprotein phosphatases (PPPs) has been challenging, but this study introduces a novel approach, MRBLE:Dephos, to determine phosphatase preferences. Using this method, the authors identified amino acid preferences of residues surrounding the dephosphorylation site for PP1 and PP2A-B55, and discovered key dephosphorylation sites during mitotic exit that are consistent with MRBLE:Dephos results.
MOLECULAR SYSTEMS BIOLOGY
(2023)
Review
Cell Biology
Vincent Archambault, Jingjing Li, Virginie Emond-Fraser, Myreille Larouche
Summary: In most animal cell types, the disassembly of the interphase nucleus during mitotic entry is well-studied, but the mechanisms of nuclear reassembly at the end of mitosis are less understood. Recent research has made progress in deciphering the roles of dephosphorylation events in promoting nuclear envelope reassembly, chromosome decondensation, kinetochore disassembly, and interphase chromatin organization. The precise roles and regulation of protein phosphatases in this process, particularly the PP1 and PP2A groups, are being elucidated.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biology
Dilara Kocakaplan, Huseyin Karaburk, Cansu Dilege, Idil Kirdok, Seyma Nur Bektas, Ayse Koca Caydasi
Summary: In this study, a novel checkpoint component Glc7-Bud14 was identified, which promotes dephosphorylation of the SPOC effector protein Bfa1. The results suggest that two mechanisms act in parallel for a robust checkpoint response, where SPOC kinase Kin4 isolates Bfa1 away from inhibitory kinase Cdc5, and Glc7-Bud14 dephosphorylates Bfa1 to fully activate the checkpoint effector.
Article
Oncology
Kripa S. Keyan, Rania Alanany, Amira Kohil, Omar M. Khan
Summary: The E3 ubiquitin ligase TRIP12 is required for efficient mitosis and completion of cell division in cancer cells. Inhibition of TRIP12 enhances Taxol-induced cell death, depending on the action of FBW7 and MCL-1. Therefore, the TRIP12/FBW7/MCL-1 axis plays a crucial role in the response of cancer cells to Taxol.
Review
Biochemistry & Molecular Biology
Fabin Dang, Li Nie, Wenyi Wei
Summary: Cell cycle progression relies on the activation of cyclin-dependent kinases, regulated in part by ubiquitin-mediated proteolysis. Dysregulated proteolytic control can lead to uncontrolled cell proliferation and tumorigenesis. Targeting E3 ubiquitin ligases involved in cell cycle regulation may offer novel therapeutic strategies for cancer treatment.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Oncology
Melody Caillot, Hadjer Miloudi, Antoine Taly, Nuria Profitos-Peleja, Juliana C. Santos, Marcelo L. Ribeiro, Elsa Maitre, Simon Saule, Gael Roue, Fabrice Jardin, Brigitte Sola
Summary: By studying the impact of XPO1 gene mutation on protein function, it was found that the mutation renders lymphoma cells more sensitive to SINEs drugs. Additionally, the mutation also leads to mistrafficking of transcription factors between the nucleus and cytoplasm, affecting the response to ibrutinib.
MOLECULAR ONCOLOGY
(2023)
Review
Cell Biology
Michela Damizia, Ludovica Altieri, Patrizia Lavia
Summary: Nuclear import receptors play crucial regulatory roles in various cellular processes such as mitosis, ciliogenesis, and phase separation. Their expression levels have significant impacts on cellular functions and disease development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Azharul Islam, Anirban Chakraborty, Stefano Gambardella, Rosa Campopiano, Altaf H. Sarker, Istvan Boldogh, Tapas Hazra
Summary: Mutation in PNKP prevents its localization in the nucleus, leading to continuous activation of the DNA damage response and neurodegeneration in AOA4 patients.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Priya Amin, Sushil Awal, Suzanne Vigneron, Sylvain Roque, Francisca Mechali, Jean Claude Labbe, Thierry Lorca, Anna Castro
Summary: PP2A is a major serine/threonine phosphatase involved in cell signaling regulation, with different complexes like PP2A-B55 having specific substrate specificity and being negatively regulated by inhibitors and upstream kinases, defining temporal patterns of substrate dephosphorylation. This review aims to compile all knowledge of PP2A-B55, a key enzyme in cell homeostasis and cancer development.
Article
Biochemistry & Molecular Biology
Florentin Huguet, Ezgi Gokan, Helen A. A. Foster, Hasnat A. A. Amin, Paola Vagnarelli
Summary: Lamin A phosphorylation and de-phosphorylation are important regulatory mechanisms during cell division, which are involved in nuclear envelope disassembly and reformation, as well as gene expression regulation. Repo-Man/PP1 phosphatase mediates the de-phosphorylation of Lamin A S22, and their interaction is mediated by SUMOylation.
Article
Cell Biology
Shao-Ming Wang, Hsiang-En Wu, Yuko Yasui, Michal Geva, Michael Hayden, Tangui Maurice, Mauro Cozzolino, Tsung-Ping Su
Summary: Autophagy is a crucial cellular process with implications in various diseases. In this study, researchers discovered that the molecular chaperone SIGMAR1 is involved in the transport of TFEB into the nucleus by chaperoning the NP protein POM121, which is responsible for recruiting KPNB1. The disruption of this process in ALS-FTD patients with the C9orf72 subtype leads to impaired autophagy. However, overexpression of SIGMAR1 or POM121, as well as treatment with pridopidine, a SIGMAR1 agonist, can rescue these deficits, suggesting their potential therapeutic use.
Review
Cell Biology
Venecia A. A. Valdez, Lila Neahring, Sabine Petry, Sophie Dumont
Summary: Spindle assembly during cell division requires the self-organization of microtubules into a complex, bipolar structure that directs the movement of chromosomes. Recent advances reveal the emergent properties of the spindle, particularly its mechanical features, that facilitate robust assembly and chromosome segregation.
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Lingyu Hu, Haokai Xu, Xiaoguang Wang, Bin Wu, Fei Chen, Wei Chen, Yong Gao, Zhengxiang Zhong
Summary: Pancreatic cancer (PAAD) is a common malignancy with a poor survival rate. High expression of PPP1CB in PAAD tissue is associated with improved overall survival and may serve as an independent prognostic indicator for clinical outcome in patients with PAAD.
Article
Oncology
Yoshihito Ohhara, Ichiro Kinoshita, Akira Suzuki, Makoto Imagawa, Jun Taguchi, Takuro Noguchi, Satoshi Takeuchi, Yasushi Shimizu, Hideyuki Seki, Junichi Suzuki, Hirotoshi Dosaka-Akita
Summary: High expression of KPNA2 and KPNB1 in gastric cancer is significantly correlated with tumor depth, lymph node metastasis, lymphatic invasion, venous invasion and Ki-67 expression, and is associated with poor prognosis in patients.
Article
Biochemistry & Molecular Biology
Magdalena Foltman, Ivan Mendez, Joan J. Bech-Serra, Carolina de la Torre, Jennifer L. Brace, Eric L. Weiss, Maria Lucas, Ethel Queralt, Alberto Sanchez-Diaz
Summary: This article investigates the negative regulatory mechanism of cell separation by TORC1 in budding yeast, revealing that TORC1 controls the process of separation by phosphorylating Cbk1 and also affects the activation of Sec3 on the SNARE complex. This discovery sheds light on the crucial role of TORC1 in cell separation.
Article
Cell Biology
Luis F. Montano-Gutierrez, Shinya Ohta, Georg Kustatscher, William C. Earnshaw, Juri Rappsilber
MOLECULAR BIOLOGY OF THE CELL
(2017)
Article
Multidisciplinary Sciences
Giulia Vargiu, Alexandr A. Makarov, James Allan, Tatsuo Fukagawa, Daniel G. Booth, William C. Earnshaw
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Multidisciplinary Sciences
Ines J. de Castro, James Budzak, Maria L. Di Giacinto, Lorena Ligammari, Ezgi Gokhan, Christos Spanos, Daniela Moralli, Christine Richardson, Jose I. de las Heras, Silvia Salatino, Eric C. Schirmer, Katharine S. Ullman, Wendy A. Bickmore, Catherine Green, Juri Rappsilber, Sarah Lamble, Martin W. Goldberg, Veronica Vinciotti, Paola Vagnarelli
NATURE COMMUNICATIONS
(2017)
Article
Biochemical Research Methods
Nicholas C. O. Lee, Jung-Hyun Kim, Nikolai S. Petrov, Hee-Sheung Lee, Hiroshi Masumoto, William C. Earnshaw, Vladimir Larionov, Natalay Kouprina
ACS SYNTHETIC BIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Alisa Zhiteneva, Juan Jose Bonfiglio, Alexandr Makarov, Thomas Colby, Paola Vagnarelli, Eric C. Schirmer, Ivan Matic, William C. Earnshaw
Review
Biochemistry & Molecular Biology
Raquel Sales Gil, Ines J. de Castro, Jerusalem Berihun, Paola Vagnarelli
BIOCHEMICAL SOCIETY TRANSACTIONS
(2018)
Article
Biochemistry & Molecular Biology
Raquel Sales Gil, Paola Vagnarelli
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2019)
Article
Multidisciplinary Sciences
Johan H. Gibcus, Kumiko Samejima, Anton Goloborodko, Itaru Samejima, Natalia Naumova, Johannes Nuebler, Masato T. Kanemaki, Linfeng Xie, James R. Paulson, William C. Earnshaw, Leonid A. Mirny, Job Dekker
Editorial Material
Biochemistry & Molecular Biology
Paola Vagnarelli, Dario R. Alessi
Editorial Material
Biochemistry & Molecular Biology
Raquel Sales Gil, Paola Vagnarelli
TRENDS IN BIOCHEMICAL SCIENCES
(2018)
Review
Cell Biology
Florentin Huguet, Shane Flynn, Paola Vagnarelli
Meeting Abstract
Cell Biology
R. Sales Gil, H. Amin, V. Vinciotti, P. Vagnarelli
MOLECULAR BIOLOGY OF THE CELL
(2018)
Meeting Abstract
Cell Biology
E. Gokhan, P. Vagnarelli
MOLECULAR BIOLOGY OF THE CELL
(2018)
Review
Biochemistry & Molecular Biology
Oscar Molina, Natalay Kouprina, Hiroshi Masumoto, Vladimir Larionov, William C. Earnshaw
Article
Biochemistry & Molecular Biology
Frances Spiller, Bethan Medina-Pritchard, Maria Alba Abad, Martin A. Wear, Oscar Molina, William C. Earnshaw, A. Arockia Jeyaprakash
