期刊
NANOSCALE RESEARCH LETTERS
卷 8, 期 -, 页码 -出版社
SPRINGER
DOI: 10.1186/1556-276X-8-72
关键词
Vaccines; Gold nanoparticles; ELISPOTs; Immunotherapy; Dendritic cells; Self-assembled monolayer
资金
- Cell and Gene Therapy Center
- Cancer Prevention Research Institute of Texas (CPRIT)
- Department of Defense Congressionally Directed Medical Research Program [USAMRAA W81XWH-07-1-0428]
- Ruth L. Kirschstein National Research Service Awards [F30CA165686]
- Medical Scientist Training Program at Baylor College of Medicine
Nanocarriers have been explored to improve the delivery of tumor antigens to dendritic cells (DCs). Gold nanoparticles are attractive nanocarriers because they are inert, non-toxic, and can be readily endocytosed by DCs. Here, we designed novel gold-based nanovaccines (AuNVs) using a simple self-assembling bottom-up conjugation method to generate high-peptide density delivery and effective immune responses with limited toxicity. AuNVs were synthesized using a self-assembling conjugation method and optimized using DC-to-splenocyte interferon-gamma enzyme-linked immunosorbent spot assays. The AuNV design has shown successful peptide conjugation with approximately 90% yield while remaining smaller than 80 nm in diameter. DCs uptake AuNVs with minimal toxicity and are able to process the vaccine peptides on the particles to stimulate cytotoxic T lymphocytes (CTLs). These high-peptide density AuNVs can stimulate CTLs better than free peptides and have great potential as carriers for various vaccine types.
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