Review
Immunology
Kristian Daniel Ralph Roth, Esther Veronika Wenzel, Maximilian Ruschig, Stephan Steinke, Nora Langreder, Philip Alexander Heine, Kai-Thomas Schneider, Rico Ballmann, Viola Fuehner, Philipp Kuhn, Thomas Schirrmann, Andre Frenzel, Stefan Duebel, Maren Schubert, Gustavo Marcal Schmidt Garcia Moreira, Federico Bertoglio, Giulio Russo, Michael Hust
Summary: Antibodies are crucial for the diagnosis and treatment of diseases caused by pathogens. Their importance has been highlighted during the COVID-19 pandemic, and new technologies like antibody phage display have advanced the discovery of human monoclonal antibodies for therapeutic purposes. These technologies have expanded the possibilities for generating antibodies against a wide range of pathogens and toxins.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biophysics
Olga I. Guliy, Stella S. Evstigneeva, Lev A. Dykman
Summary: Antibody phage display is a useful technique for producing antibodies to specific antigens, replacing the traditional hybrid-oma technology. It simplifies the process by manipulating DNA and bacteria, shortening the time and reducing the cost of antibody production. Antibodies prepared through phage display can be used as selective receptors in biosensors.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Microbiology
Shuai Hu, Guangbo Yang, Zhou Chen, Qiuye Li, Bin Liu, Ming Liu, Dawei Zhang, Shan Chang, Ren Kong
Summary: This study presents a method combining docking and site specific phage display to obtain single chain variable fragment (scFv) with high affinity to Salbutamol (SAL). Anti-SAL scFv antibodies were generated through phage display and new libraries were created by randomizing specific residues located on H3 and L3 based on docking results. High efficiency anti-SAL scFv antibodies were identified and used to develop a rapid and low-cost one step ELISA for SAL detection.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Yaqi Yang, Siji Nian, Lin Li, Xue Wen, Qin Liu, Bo Zhang, Yu Lan, Qing Yuan, Yingchun Ye
Summary: Enhanced EphA2 expression is a crucial target for anti-tumor therapy, with high affinity scFvs against EphA2 successfully screened from an immune library constructed using PBMCs from cancer patients. Modified antibodies showed improved affinity and the ability to bind to EphA2, tumor cells, and tissues, inhibiting tumor growth to some extent. This study highlights the potential of immune libraries from cancer patients in screening for high-affinity antibodies with therapeutic effects.
Article
Food Science & Technology
Hugo Valencia-Martinez, Timoteo Olamendi-Portugal, Rita Restano-Cassulini, Hugo Serrano-Posada, Fernando Zamudio, Lourival D. Possani, Lidia Riano-Umbarila, Baltazar Becerril
Summary: Centruroides huichol scorpion venom is lethal to mammals. In this study, four lethal toxins were characterized and a scFv HV was developed with improved affinity to Chui5 toxin. The scFv HV showed promising results in a rescue trial, protecting mice from lethal doses of venom. This research contributes to the development of a recombinant anti-venom against scorpion stings in Mexico.
Review
Biochemical Research Methods
Rui Wang, Hui-Da Li, Ying Cao, Zi-Yi Wang, Ting Yang, Jian-Hua Wang
Summary: Viruses, specifically M13 phage, are being utilized in biosensing and biomedicine due to their versatility, specific reactivities, and responsiveness to genetic modifications. M13 phages can be engineered and modified to create a multifunctional analysis platform, with different functional regions performing their tasks without interference. The unique filamentous morphology and flexibility of M13 phages enhance target affinity and signal amplification in analytical applications.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Wei Sun, Yan Zhang, Zhigang Ju
Summary: The use of phage display technology in mycotoxins detection has shown promising developments, providing novel choices for antibodies or hapten conjugates with simplicity, ease of use, and lower cost, making them environmentally friendly immune-reagents for the next generation of immunoassays.
Article
Fisheries
Haneul Seo, Andre Ditya Maulana Lubis, Tae-Jin Choi, Tae-Sung Jung, Taek-Kyun Lee, Sukchan Lee
Summary: Koi herpesvirus (KHV) is a highly contagious virus that causes high mortality in koi and common carp. Current diagnostic methods cannot detect KHV virus particles, so researchers have proposed a method using specific antibodies to detect the virus. The study found that F6 and C5 scFvs have sufficient binding affinity for KHV particles and can be used in immunoassays.
Review
Food Science & Technology
Long Li, Shuangmin Wu, Yu Si, Huaming Li, Xiaoyang Yin, Dapeng Peng
Summary: The single-chain fragment variable (scFv) is a reliable, efficient, and accurate method for analyzing small-molecule harmful substances in food. It offers advantages over traditional antibodies and can be further improved through efficient construction, mutation, and expression measures.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2022)
Article
Biochemistry & Molecular Biology
Jing Yi Lai, Angela Corona, Chong Lee Ng, Enzo Tramontano, Yee Siew Choong, Theam Soon Lim
Summary: Ebola virus is notorious for causing severe and deadly haemorrhagic fever. Effective diagnosis and treatment are needed due to the high fatality rate. Two monoclonal antibodies have been approved for EVD treatment. The viral surface glycoprotein is a common target, but VP35 could be a potential target as well.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Microbiology
Chenglong Ma, Chunyang Jiang, Dongping Zhao, Shuhao Li, Ronggui Li, Lei Li
Summary: Directed evolution is a widely-used engineering strategy for improving protein stability or biochemical functions through repeated rounds of mutation and selection. Using ELISA to check the displayed protein against the target and enhancing the signal through various approaches can improve the sensitivity of ELISA experiments when dealing with low-affinity ligand-protein interactions.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Pierre-Emmanuel Baurand, Jeremy Balland, Chloe Reynas, Melanie Ramseyer, Delphine Vivier, Pierre-Simon Bellaye, Bertrand Collin, Catherine Paul, Franck Denat, Kamal Asgarov, Jean-Rene Pallandre, Laurence Ringenbach
Summary: The study developed an ScFv mouse phage-display library to obtain small fragment antibodies against human LRRC15 for molecular imaging purposes, demonstrating the relevance for the fast-track development of small antibodies for imaging and/or immunotherapy purposes. The library had a high insertion rate and enrichment through screening, leading to the identification of specific candidates for further development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Eeva-Christine Brockmann, Mikko Pyykko, Heidi Hannula, Kamran Khan, Urpo Lamminmaki, Tuomas Huovinen
Summary: This study discusses the impact of loop length variation on the structural diversity of antibody libraries. Introducing loop length variation in specific antibody fragments can enhance affinity, especially in CDR-L1 and CDR-H2 loops. The results lay the foundation for developing novel synthetic antibody libraries with unique loop length combinations.
Article
Microbiology
Zamrina Baharudeen, Rahmah Noordin, Lim Theam Soon, Dinesh Balachandra, Nor Suhada Anuar, Fatin Hamimi Mustafa, Anizah Rahumatullah
Summary: This study successfully isolated and produced novel recombinant monoclonal antibodies against Toxocara canis recombinant TES-26 antigen. The antibodies showed high specificity and sensitivity, and demonstrated potential diagnostic and therapeutic applications.
Article
Nanoscience & Nanotechnology
Ji Hong Kim, Hye-Seon Jeong, Jaehyeon Hwang, Dae-Hyuk Kweon, Chang-Hyung Choi, Jong Pil Park
Summary: A sensor based on affinity peptide-immobilized hydrogel microspheres has been developed for the selective detection of influenza A virus. The sensor shows rapid and accurate detection capability, which is crucial for the effective control of pandemic flu outbreaks.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Virology
Binod Kumar, Arunava Roy, Kumari Asha, Neelam Sharma-Walia, Mairaj Ahmed Ansari, Bala Chandran
JOURNAL OF VIROLOGY
(2019)
Article
Infectious Diseases
Jitendra K. Biswal, Saravanan Subramaniam, Rajeev Ranjan, Kimberly VanderWaal, Aniket Sanyal, Brahmadev Pattnaik, Raj Kumar Singh
INFECTION GENETICS AND EVOLUTION
(2020)
Article
Virology
Mohanan Valiya Veettil, Gayathri Krishna, Arunava Roy, Anandita Ghosh, Dipanjan Dutta, Binod Kumar, Sayan Chakraborty, Anju Thoppil Raveendran, Neelam Walia, Bala Chandran
JOURNAL OF VIROLOGY
(2020)
Article
Microbiology
Shaheer Ahmad, Debapriya Bhattacharya, Neeta Gupta, Varsha Rawat, Sultan Tousif, Luc Van Kaer, Gobardhan Das
Article
Veterinary Sciences
G. Govindaraj, Ganesh Kumar, A. Krishnamohan, Raveendra Hegde, Nanda Kumar, Kokila Prabhakaran, Vinay Mohan Wadhwan, Naresh Kakker, T. Lokhande, Krishna Sharma, Amit Kanani, Limaye, K. Natchimuthu, P. N. Ananth, Arup Kumar De, Tanveer Ahmed Khan, Jyoti Misri, B. B. Dash, Rahman Habibur
Summary: The study investigated the important epidemiological parameters and farm-level economic costs of FMD incidence in cattle and buffaloes during 2013-14 to 2015-16 in various states of India. The highest number of affected villages and disease incidence was observed in non-FMD control programme implemented Madhya Pradesh and Assam states, respectively, whereas negligible incidence was in FMD-CP implemented Punjab state. The disease incidence was high during 2013-14 and declined during 2014-15 and 2015-16, respectively implied severe incidence scenario (2013-14) succeeded by moderate (2014-15) and mild (2015-16) scenarios.
PREVENTIVE VETERINARY MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Sonalika Mahajan, Gaurav Kumar Sharma, Kavita Bora, Bramhadev Pattnaik
Summary: This study identified host proteins interacting with FMDV 2C protein using the Y2H approach, revealing both known and novel interactions that enhance understanding of the 2C-host interface and may offer insights for potential therapeutics against FMDV.
JOURNAL OF GENERAL VIROLOGY
(2021)
Article
Cell Biology
Binod Kumar, Grant M. Hawkins, Tom Kicmal, Enya Qing, Emily Timm, Tom Gallagher
Summary: Researchers have developed and utilized a VLP system to identify features of SARS-CoV2 assembly and entry. They found that N proteins are crucial for VLP production and secretion, and the CTDs in virus particle assembly are critical features. Furthermore, VLPs were effective at monitoring virus-cell entry, depending on host cell susceptibility factors.
Article
Microbiology
Enya Qing, Tom Kicmal, Binod Kumar, Grant M. Hawkins, Emily Timm, Stanley Perlman, Tom Gallagher
Summary: Adaptive changes in the coronavirus spike proteins, especially in the N-terminal domains, can increase SARS-CoV-2 spike protein-mediated membrane fusion and cell entry, leading to amplified virus-cell binding. However, these changes may also reduce interdomain associations, making spike proteins susceptible to irreversible inactivation on virus-like particles.
Editorial Material
Immunology
Binod Kumar, Yean K. Yong, Vikas Sood
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Akshaya Ramachandran, Binod Kumar, Gulam Waris, David Everly
Summary: This study reveals that HCV-induced activation of the NLRP3 inflammasome in hepatocytes is regulated by posttranslational modifications, specifically deubiquitination by UCHL5. Inhibition of deubiquitination blocks NLRP3 inflammasome activation, IL-1 beta maturation, and reduces HCV replication. This highlights the crucial role of the NLRP3 inflammasome in HCV pathogenesis and suggests potential therapeutic targets for managing HCV infection.
MICROBIOLOGY SPECTRUM
(2021)
Article
Agriculture, Dairy & Animal Science
G. Govindaraj, Ganesh B. Kumar, A. Krishnamohan, Raveendra Hegde, S. Nandakumar, Kokila Prabhakaran, Vinay Mohan, Naresh Kakker, T. Lokhande, Krishna Sharma, Amit Kanani, A. Limaye, K. Natchimuthu, Tanveer Ahmed Khan, Jyoti Misri, B. B. Dash, B. Pattnaik, H. Rahman
INDIAN JOURNAL OF ANIMAL SCIENCES
(2020)
Review
Agriculture, Dairy & Animal Science
Sharanagouda S. Patil, Kuralayanapalya Puttahonnappa Suresh, Vikram Vashist, Awadhesh Prajapati, Bramhadev Pattnaik, Parimal Roy
Article
Microbiology
Miranda R. Bertram, Rachel M. Palinski, Steven J. Pauszek, Ethan J. Hartwig, George R. Smoliga, Jitendra K. Biswal, Rajeev Ranjan, Saravanan Subramaniam, Jajati K. Mohapatra, Biswajit Das, Ian H. Fish, Bramhadev Pattnaik, Luis L. Rodriguez, Jonathan Arzt
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2020)
Article
Agriculture, Dairy & Animal Science
S. D. Audarya, A. Sanyal, J. K. Mohapatra, L. K. Pandey, R. Kumar, M. Kankipati, Y. Shanmugam, A. Jangham, N. B. Singanallur, B. Pattnaik
Letter
Immunology
Devendra Mourya, Pragya Yadav, Manoranjan Rout, Brahmdev Pattnaik, Anita Shete, Deepak Patil
INDIAN JOURNAL OF MEDICAL RESEARCH
(2019)