Article
Biotechnology & Applied Microbiology
Sophie Steinhagen, Louise Kramar, Gunilla B. Toth
Summary: The identification of tube-forming species in the genus Ulva is often problematic due to insufficient genetic and morphological data. A new species, Ulva capillata, was identified and described based on molecular and morphological evidence, highlighting the importance of molecular investigation for accurate species identification in this genus.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Sophie Steinhagen, Samanta Hoffmann, Henrik Pavia, Gunilla B. Toth
Summary: Correct species identification is crucial for biodiversity assessment and conservation management. This study used molecular monitoring to analyze the species diversity and distribution of the green algal genus Ulva along the Atlantic-Baltic Sea salinity gradient. The study revealed the presence of 20 genetic entities, with 11 identified to species level. The presence of non-native species highlights the need for further monitoring to mitigate the formation of green tides.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Biodiversity Conservation
Robert J. Mrowicki, Juliet Brodie
Summary: This study reports the presence of a non-native seaweed, Ulva fenestrata, in the sub-Antarctic island of South Georgia and confirms its widespread presence in the Falkland Islands using molecular-assisted taxonomy. This is the second Southern Hemisphere record of U. fenestrata, emphasizing the importance of monitoring and robust biosecurity measures in South Georgia.
Article
Biochemistry & Molecular Biology
I. Gede Putu Wirawan, Ni Kadek Emi Sintha Dewi, Maria Malida Vernandes Sasadara, I. Gde Nengah Adhilaksman Sunyamurthi, I. Made Jawi, I. Nyoman Wijaya, Ida Ayu Putri Darmawati, I. Ketut Suada, Anak Agung Keswari Krisnandika
Summary: This study aimed to identify the indigenous Caulerpa algae, Bulung Boni and Bulung Anggur, and analyze their bioactive components. The results revealed the close relationship between Bulung Boni and Caulerpa cylindracea, while Bulung Anggur showed no close relationship to other Caulerpa species. GC-MS analysis identified multiple bioactive compounds with antioxidant, antibacterial, anticancer, anti-inflammation, and antidiabetic properties in these algae.
Article
Plant Sciences
Jianli Wang, Zhenfei Yan, Peng Zhong, Zhongbao Shen, Guofeng Yang, Lichao Ma
Summary: This study evaluated the utility of four candidate DNA barcoding regions in seven genera of Gramineae. It found that matK and ITS showed the highest discriminatory power, while rbcL and trnL-F were less effective. The study also demonstrated the feasibility of establishing a technical system for rapid and reliable identification of these seven genera, and provided crucial reference for analyzing the genetic diversity of grass germplasm resources.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Environmental Sciences
Alejandro Nistal-Garcia, Pedro Garcia-Garcia, Jorge Garcia-Giron, Maria Borrego-Ramos, Saul Blanco, Eloy Becares
Summary: The study compares the effectiveness of traditional morphological methods and DNA barcoding methods in studying diatom communities. The results show significant differences in the compositional variation of diatom metacommunities between the two approaches. Biases affecting the molecular approach may include incomplete reference databases and bioinformatics processing, while biases affecting the morphological method may be limited counting effort and the presence of cryptic species. Additionally, variation in diatom community composition is strongly related to the environmental template, but not significantly related to geographical distances.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biology
Awad A. Algarni
Summary: This study is the first to document the rare plant Aloe shadensis using genomic markers. It found that the matK gene and ITS region sequences were effective in identifying the plant.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Biodiversity Conservation
Gabrielle M. Kuba, Brenda Carpio-Aguilar, Jason Eklund, D. Wilson Freshwater
Summary: This study aimed to determine the diversity of Ulva species in the San Juan Islands using DNA barcoding. Four species were identified through sequencing and cluster analysis, and their identities were confirmed using holotype and topotype specimens. Additional sequencing efforts are needed to accurately identify more Ulva species and understand their true biodiversity.
Article
Plant Sciences
James T. Melton, Juan M. Lopez-Bautista
Summary: Ulva is a common algae in intertidal and freshwater ecosystems, with difficulties in morphological identification due to cryptic diversity and morphological plasticity. Molecular data has helped to better understand its diversity. A study in the Gulf of Mexico and Atlantic USA identified 16 Ulva clades, including 13 named clades and potentially three undescribed species. Only nine of the previously reported 24 Ulva taxa in the Western Atlantic were confirmed, with four species identified for the first time in the U.S. East and Gulf Coast.
JOURNAL OF PHYCOLOGY
(2021)
Article
Plant Sciences
Xiaomeng Mao, Wei Xie, Xinnian Li, Suhua Shi, Zixiao Guo
Summary: Mangrove ecosystems in China are under threat, and DNA barcoding technology can help in rapid species identification to enhance conservation efforts.
Article
Biotechnology & Applied Microbiology
Lan-Anh T. Tran, Christophe Vieira, Sophie Steinhagen, Christine A. Maggs, Masanori Hiraoka, Satoshi Shimada, Tu Van Nguyen, Olivier De Clerck, Frederik Leliaert
Summary: This study assessed different perspectives to establish a stable taxonomic framework for Ulva green seaweed, finding that species delimitation methods using the tufA gene were the most consistent. DNA-based species delimitation often disagreed with traditional morphology-based definitions, and biological species concepts were generally narrower than DNA-based delimitation. Although molecular markers improved our understanding of Ulva diversity, names associated with DNA sequences in public databases are unreliable, highlighting the need for further research in tropical regions.
JOURNAL OF APPLIED PHYCOLOGY
(2022)
Article
Environmental Sciences
Shanmei Zou, Yachao Bao, Xuemin Wu, Changhai Wang
Summary: The study evaluated the efficiency of four genetic markers in barcoding diatoms isolated from China, showing that multiple genetic markers can clearly differentiate most species and reveal potential cryptic species. COI, rbcL, and LSU sequences demonstrated high taxonomic resolution at the species level, while potential identification errors were found in public diatom sequences. Phylogenetic and character-based analysis provided consistent species identification and clearer species discrimination compared to the distance-based method.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Ecology
Jie Wang, Jinquan Zhao, Weisen Yu, Shuangyu Wang, Shuhua Bu, Xuexiang Shi, Xiuyun Zhang
Summary: The study suggests that DNA barcoding is a rapid and accurate method for identifying and tracing toxic plants. RbcL is recommended as the best single marker for clinical identification of toxic plants, and the BLAST method is suggested for analysis.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2021)
Article
Plant Sciences
Marcella Guennes Tavares de Oliveira, Sonia Maria Barreto Pereira, Ana Maria Benko-Iseppon, Valdir Queiroz Balbino, Wilson Jose da Silva, Caroline Feijao Ximenes, Maria de Fatima de Oliveira Carvalho, Valeria Cassano
Summary: This study evaluated the diversity of Bryopsis in northeastern Brazil using morphological and molecular data, finding incongruence between morphology and molecular analysis, indicating the presence of cryptic and polymorphic species in the genus. The research demonstrated that Bryopsis pennata is an extremely plastic species inclusive of specimens with morphotype B. plumosa, and also identified Bryopsis pennata var. secunda for the first time in northeastern Brazil.
ACTA BOTANICA BRASILICA
(2021)
Article
Plant Sciences
Almuthanna K. Alkaraki, Maisam A. Aldmoor, Jamil N. Lahham, Mohammed Awad
Summary: The study aimed to develop a DNA barcoding method to identify and characterize Daphne mucronata Royle and Thymelaea hirsuta plant species. The research found that DNA barcoding successfully identified the two plant species using chloroplast genes.