期刊
JOURNAL OF OCEAN UNIVERSITY OF CHINA
卷 13, 期 1, 页码 153-156出版社
OCEAN UNIV CHINA
DOI: 10.1007/s11802-014-2011-0
关键词
fucoidan degradation; Flavobacteriaceae; 16S rDNA; HPSEC
类别
资金
- National Natural Science Foundation of China [30800858]
Fucoidan, a polysaccharide containing abundant fucose and sulfate ester group, was prepared from Laminaria japonica. In order to obtain fucoidan-degrading enzyme, bacteria capable of degrading fucoidan were screened from kelp. A bacterial strain named RC2-3 was obtained, which degraded fucoidan by the maximum extent of 54% +/- 1.3%, the highest among all bacterial isolates. High-performance size exclusion chromatography (HPSEC) showed that the molecular weight of fucoidan was gradually reduced by RC2-3 with culturing time, suggesting the production of fucoidan-degrading enzyme by RC2-3. Phylogenetic analysis of partial 16S ribosomal RNA gene (16S rDNA) sequence showed that RC2-3 belonged to the family Flavobacteriaceae. However, it showed different physiological and biochemical characteristics from the known Flavobacteriaceae members producing fucoidan-degrading enzyme, thus RC2-3 was proposed to be a new member of this family.
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