期刊
JOURNAL OF NANOBIOTECHNOLOGY
卷 11, 期 -, 页码 -出版社
BMC
DOI: 10.1186/1477-3155-11-17
关键词
Gold nanoclusters; Silica; Folic acid; Fluorescent imaging; X-ray computed tomography
资金
- National Key Basic Research Program (973 Project) [2010CB933901, 2011CB933100]
- National 863 Hi-tech Project [2007AA022004]
- Important National Science & Technology Specific Projects [2009ZX10004-311]
- National Natural Scientific Fund [81225010, 20803040]
- Special Project for Nanotechnology from Shanghai [1052 nm04100]
- Shanghai Science and Technology Fund [10XD1406100]
- Shanghai Jiao Tong University Innovation Fund for Postgraduates
Background: Gastric cancer is 2th most common cancer in China, and is still the second most common cause of cancer-related death in the world. Successful development of safe and effective nanoprobes for in vivo gastric cancer targeting imaging is a big challenge. This study is aimed to develop folic acid (FA)-conjugated silica coated gold nanoclusters (AuNCs) for targeted dual-modal fluorescent and X-ray computed tomography imaging (CT) of in vivo gastric cancer cells. Method: AuNCs were prepared, silica was coated on the surface of AuNCs, then folic acid was covalently anchored on the surface of AuNCs, resultant FA-conjugated AuNCs@SiO2 nanoprobes were investigated their cytotoxicity by MTT method, and their targeted ability to FR(+) MGC803 cells and FR(-) GES-1 cells. Nude mice model loaded with MGC803 cells were prepared, prepared nanoprobes were injected into nude mice via tail vein, and then were imaged by fluorescent and X-ray computed tomography (CT) imaging. Results: FA-conjugated AuNCs@SiO2 nanoprobes exhibited good biocompatibility, and could target actively the FR(+) MGC-803 cells and in vivo gastric cancer tissues with 5 mm in diameter in nude mice models, exhibited excellent red emitting fluorescence imaging and CT imaging. Conclusion: The high-performance FA-conjugated AuNCs@SiO2 nanoprobes can target in vivo gastric cancer cells, can be used for fluorescent and CT dual-mode imaging, and may own great potential in applications such as targeted dual-mode imaging of in vivo early gastric cancer and other tumors with FR positive expression in near future.
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