Article
Nanoscience & Nanotechnology
Xingxing Jiang, Minghui Yang, Juewen Liu
Summary: This study systematically investigated the effect of surface-modified gold nanoparticles (AuNPs) on loop-mediated isothermal amplification (LAMP). It was found that mixing AuNPs with primers delayed the reaction, while other mixing orders accelerated it. The performance of AuNPs on LAMP was most significant when they formed a protein-like surface. By using glutathione-capped AuNPs, a detection limit of around 100 copies/μL of target DNA was achieved, indicating a higher sensitivity in DNA sensing.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Huan-Huan Zhu, Yuan Li, Li-Xia Wu, Ke-Sheng Wang, Yue Zhang, Qiang-Yuan Fan, Zun-Zhen Ming, Wei-Qin Chen, Wei-Wei Liu
Summary: Loop-mediated isothermal amplification (LAMP) is a promising diagnostic tool for genetic amplification, known for its speed, simplicity, efficiency, and sensitivity. This research introduces an internal heating method for LAMP using magnetic nanoparticles (MNPs) called nano-LAMP, which can detect low concentrations of HPV and has been validated in clinical samples.
Review
Microbiology
Cassandra Suther, Sloane Stoufer, Yanjiao Zhou, Matthew D. Moore
Summary: Foodborne and enteric viruses impose a significant burden on public health and economy globally. Real time PCR, although considered a gold standard, has limitations in terms of portability, sample processing, and time to result. Isothermal amplification methods have shown promise as portable, sensitive, and rapid alternatives to PCR for detecting these viruses.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Alfonsina Fiore, Ida Treglia, Gianni Ciccaglioni, Marco Francesco Ortoffi, Antonietta Gattuso
Summary: Changing eating habits and rising demand of food have led to an increase in foodborne diseases, particularly in industrialized countries. Contaminated ready-to-eat food can transmit Listeria monocytogenes, a foodborne pathogen responsible for listeriosis. This study successfully applied a colorimetric Loop-mediated isothermal amplification (LAMP) assay to rapidly detect L. monocytogenes in experimentally contaminated RTE meat samples. The results showed high specificity and sensitivity compared to traditional methods, suggesting that colorimetric LAMP assays can be used as a screening tool for L. monocytogenes in ready-to-eat meat food.
Article
Immunology
Xingyun Wang, Guirong Wang, Yacui Wang, Shuting Quan, Hui Qi, Lin Sun, Chen Shen, Hairong Huang, Weiwei Jiao, Adong Shen
Summary: The study developed a simple and reliable method for detecting Mycobacterium tuberculosis complex (MTBC) using a multiplex LAMP-LFB assay, which demonstrated higher sensitivity than traditional culture and Xpert MTB/RIF tests, especially in resource limited settings and probable TB cases.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Xiaoqing Tong, Jiayi Wu, Li Mei, Yongjun Wang
Summary: This study investigated the dieback and basal stem necrosis disease in Chinese hickory plantations in China, resulting in a high incidence rate. Through molecular detection and loop-mediated isothermal amplification technology, a convenient tool was developed for field monitoring of the pathogenic Phytophthora cinnamomi.
Article
Biology
Sara M. Elnomrosy, Naglaa M. Hagag, Mohamed I. AbdAllah, Rafal Kolenda, Maciej Zacharski
Summary: This study compared the performance of LAMP and PCR techniques for sex identification of 21 parrot species, showing that LAMP, especially with PSI-W primers, is suitable for previously untested species and is more sensitive for female sex identification.
Article
Plant Sciences
Qingcong He, Dongwei Wang, Bei Tang, Jian Wang, Deyong Zhang, Yong Liu, Feixue Cheng
Summary: In this study, specific primers were designed for rapid identification and quantification of the plant-parasitic nematode Meloidogyne graminicola in soil. Both LAMP and real-time PCR showed higher sensitivity in detecting the nematode compared to conventional PCR. These methods have the potential to provide a useful platform for rapid species identification and quantification of economically important PPNs.
Article
Biochemical Research Methods
Yao Lu, Ma-chao Li, Hai-can Liu, Shi-qiang Lin, Xiu-qin Zhao, Zhi-guang Liu, Li-li Zhao, Kang-Lin Wan
Summary: The new detection method, combining multiple technologies, can detect MTBC and RIF resistance rapidly, simply, and accurately, with high specificity and sensitivity. This method does not require special equipment, is easy to operate, and is suitable for resource-limited environments.
ANALYTICAL BIOCHEMISTRY
(2021)
Review
Chemistry, Analytical
So-Hee Kim, So-Young Lee, Unji Kim, Se-Wook Oh
Summary: This review outlines several techniques for reducing false-positive LAMP results before and after amplification. Organic additives or pullulan can be used to decrease nonspecific amplification before amplification. Uracil-DNA-glycosylase and the hot-start effect with gold nanoparticles can eliminate carry-over contamination and reduce nonspecific amplification after amplification. The use of guide RNA and colorimetric change in DNAzyme can accurately confirm amplification results.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Anto J. U. K. John, Peijun J. W. He, Ioannis N. Katis, P. P. Galanis, Alice H. Iles, Robert W. Eason, Collin L. Sones
Summary: With the ongoing SARS-CoV-2 pandemic, the development of rapid and cost-effective diagnostic methods has become crucial. A testing strategy utilizing a sensitive RT-LAMP assay in commercially available glass capillaries has been developed, providing results in a short timeframe and reducing the burden on testing supply chains.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Multidisciplinary
Xianjing Zhang, Jun Guo, Bo Song, Feng Zhang
Summary: The polymerase chain reaction (PCR) is widely used in medical diagnosis and forensic identification. In this study, we discovered that metal ions can improve the specificity and yield of PCR amplification. We identified a spatiotemporal regulation mechanism of metal ions in PCR and proposed a new strategy for designing regulation kits for PCR-based biomedical applications.
Review
Materials Science, Multidisciplinary
Nazente Atceken, Muhammad Munzer Alseed, Sajjad Rahmani Dabbagh, Ali K. Yetisen, Savas Tasoglu
Summary: LAMP technology is a fast, efficient, and low-cost diagnostic method for pathogen detection, which can be applied to various bacteria, viruses, and parasitic pathogens. In addition, LAMP technology can also be used for SNP genotyping and mutation detection.
ADVANCED ENGINEERING MATERIALS
(2023)
Review
Cell Biology
Marianna Soroka, Barbara Wasowicz, Anna Rymaszewska
Summary: LAMP is a rapid amplification method that can produce up to 10(9) copies of amplified DNA within less than an hour, with high specificity and stable temperature operation. It can detect products faster than standard techniques and has the potential to be a rapid diagnostic tool for SARS-CoV-2.
Review
Chemistry, Analytical
Jee-Woong Park
Summary: Nucleic acids are crucial biomarkers for pathogen-mediated diseases and viruses. Polymerase chain reaction (PCR) is the gold standard for nucleic acid-based diagnostic applications, but its requirements limit its use as a rapid point-of-care technique. To overcome the challenges associated with PCR, many isothermal amplification methods have been developed for accurate nucleic acid detection. Among these methods, loop-mediated isothermal amplification (LAMP) is the most widely studied because of its rapid and specific amplification of nucleic acids.
Article
Pharmacology & Pharmacy
Margarida Ferreira-Silva, Catarina Faria-Silva, Manuela C. Carvalheiro, Sandra Simoes, H. Susana Marinho, Paulo Marcelino, Maria Celeste Campos, Josbert M. Metselaar, Eduarda Fernandes, Pedro V. Baptista, Alexandra R. Fernandes, Maria Luisa Corvo
Summary: A stable quercetin liposomal formulation was developed and characterized in this study. The anti-inflammatory potential of liposomal quercetin was evaluated in vitro and in vivo, demonstrating its effectiveness in treating hepatic IRI.
Editorial Material
Respiratory System
Sophia B. Georghiou, Timothy C. Rodwell, Alexei Korobitsyn, Said H. Abbadi, Kanchan Ajbani, Jan-Willem Alffenaar, David Alland, Nataly Alvarez, Sonke Andres, Elisa Ardizzoni, Alexandra Aubry, Rossella Baldan, Marie Ballif, Ivan Barilar, Erik C. Bottger, Soumitesh Chakravorty, Pauline M. Claxton, Daniela M. Cirillo, Inaki Comas, Chris Coulter, Claudia M. Denkinger, Brigitta Derendinger, Edward P. Desmond, Jurriaan E. M. de Steenwinkel, Keertan Dheda, Andreas H. Diacon, David L. Dolinger, Kelly E. Dooley, Matthias Egger, Maha R. Farhat, Lanfranco Fattorini, Iris Finci, Laure Fournier Le Ray, Victoria Furio, Ramona Groenheit, Tawanda Gumbo, Scott K. Heysell, Doris Hillemann, Harald Hoffmann, Po-Ren Hsueh, Yi Hu, Hairong Huang, Alamdar Hussain, Farzana Ismail, Kiyohiko Izumi, Tomasz Jagielski, John L. Johnson, Priti Kambli, Kone Kaniga, G. H. R. Eranga Karunaratne, Meenu Kaushal Sharma, Peter M. Keller, Ellis C. Kelly, Margarita Kholina, Mikashmi Kohli, Katharina Kranzer, Ian F. Laurenson, Jason Limberis, S-Y Grace Lin, Yongge Liu, Alexandre Lopez-Gavin, Anna Lyander, Diana Machado, Elena Martinez, Faisal Masood, Satoshi Mitarai, Nomonde R. Mvelase, Stefan Niemann, Vladyslav Nikolayevskyy, Florian P. Maurer, Matthias Merker, Paolo Miotto, Shaheed Omar, Ralf Otto-Knapp, Moises Palaci, Juan Jose Palacios Gutierrez, Sharon J. Peacock, Charles A. Peloquin, Jennifer Perera, Catherine Pierre-Audigier, Suporn Pholwat, James E. Posey, Therdsak Prammananan, Leen Rigouts, Jaime Robledo, Neesha Rockwood, Camilla Rodrigues, Max Salfinger, Marcos C. Schechter, Marva Seifert, Sarah Sengstake, Thomas Shinnick, Natalia Shubladze, Vitali Sintchenko, Frederick Sirgel, Sulochana Somasundaram, Timothy R. Sterling, Andrea Spitaleri, Elizabeth Streicher, Philip Supply, Erik Svensson, Elisa Tagliani, Sabira Tahseen, Akiko Takaki, Grant Theron, Gabriela Torrea, Armand Van Deun, Annelies Van Rie, Dick van Soolingen, Roger Vargas, Amour Venter, Nicolas Veziris, Cristina Villellas, Miguel Viveiros, Robin Warren, Shu'an Wen, Jim Werngren, Robert J. Wilkinson, Caie Yang, Tingting Zhang, Danila Zimenkov, Nazir Ismail, Thomas Schon, Claudio U. Koser, Soudeh Ehsani, Jakko van Ingen, F. Ferda Yilmaz
EUROPEAN RESPIRATORY JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Daniela Alexandre, Bernardo Teixeira, Andre Rico, Salete Valente, Ana Craveiro, Pedro Baptista, Carla Cruz
Summary: Lung cancer is the leading cause of cancer-related death worldwide. Non-small cell lung cancer diagnosis and treatment have advanced, but more sensitive and specific screening methods are needed. New molecular biomarkers, particularly miRNA, are emerging as potential non-invasive diagnostic agents. We developed a molecular beacon-based miRNA detection strategy for non-small cell lung cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Nanoscience & Nanotechnology
Beatriz B. Oliveira, Daniela Ferreira, Alexandra R. Fernandes, Pedro Viana Baptista
Summary: Advances in nanotechnology and medical science have led to the development and application of nanomaterials and nanoparticles in biomedicine. Gold nanoparticles have attracted significant interest due to their unique properties, and they hold great value for use in biodetection and diagnostics.
WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY
(2023)
Article
Infectious Diseases
Mariana Andrade, Ketlyn Oliveira, Catarina Morais, Patricia Abrantes, Constanca Pomba, Adriana E. Rosato, Isabel Couto, Sofia Santos Costa
Summary: This study reveals the frequent biofilm production by antimicrobial-resistant coagulase-positive staphylococci in companion animals, which is associated with a higher virulence potential and persistent or recurrent infections.
Article
Biochemistry & Molecular Biology
Carolina Ferreira, Patricia Abrantes, Sofia Santos Costa, Miguel Viveiros, Isabel Couto
Summary: This study identified the variability of norA within Staphylococcus aureus and its distribution among other staphylococci. It also demonstrated the applicability of a PCR-based algorithm to detect and differentiate norA alleles, and found the association of norA alleles with specific S. aureus clonal lineages.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Beatriz B. Oliveira, Beatriz Costa, Barbara Morao, Sandra Faias, Bruno Veigas, Lucilia Pebre Pereira, Cristina Albuquerque, Rui Maio, Marilia Cravo, Alexandra R. Fernandes, Pedro Viana Baptista
Summary: The success of personalized medicine relies on biomarker discovery for identifying patients who will benefit from specific targeted drugs. Liquid biopsies, particularly the analysis of circulating tumor DNA, are emerging as a useful tool for diagnosis, prognosis, and biomarker discovery. In this study, an ARMS-HRMA method was developed for detecting KRAS mutations, showing simplicity, shorter time to result, high sensitivity, and specificity compared to traditional sequencing and digital PCR methods. The proposed ARMS-HRMA method has the potential to improve liquid biopsy-based diagnosis and prognosis schemes.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Infectious Diseases
Gabrielle Froberg, Florian P. Maurer, Erja Chryssanthou, Louise Fernstrom, Hanaa Benmansour, Samira Boarbi, Anne Torunn Mengshoel, Peter Michael Keller, Miguel Viveiros, Diana Machado, Margaret M. Fitzgibbon, Simone Mok, Jim Werngren, Daniela Maria Cirillo, Fernando Alcaide, Hanne-Leena Hyyrylainen, Alexandra Aubry, Sonke Andres, Darshaalini Nadarajan, Erik Svensson, John Turnidge, Christian G. Giske, Gunnar Kahlmeter, Emmanuelle Cambau, Jakko van Ingen, Thomas Schon
Summary: The study evaluated the minimum inhibitory concentration (MIC) distributions of non-tuberculous mycobacteria (NTM) to establish antimicrobial susceptibility testing (AST) breakpoints. MIC distributions for drugs against Mycobacterium avium complex (MAC) and Mycobacterium abscessus (MAB) were collected from 12 laboratories. Epidemiological cut-off values (ECOFFs) and tentative ECOFFs (TECOFFs) were determined, highlighting the need for further method refinement in establishing clinical breakpoints for NTM.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Biochemistry & Molecular Biology
Ana F. Amendoeira, Andre Luz, Ruben Valente, Catarina Roma-Rodrigues, Hasrat Ali, Johan E. van Lier, Fernanda Marques, Pedro V. Baptista, Alexandra R. Fernandes
Summary: This study evaluated the cell uptake of Estradiol-BODIPY, 19-nortestosterone-BODIPY, and testosterone-BODIPY in breast cancer and prostate cancer cells, as well as in normal dermal fibroblasts. The results showed that the highest internalization occurred in cells expressing specific receptors for the compounds. Blocking experiments also revealed differences in non-specific cell uptake between cancer and normal cells, possibly due to differences in lipophilicity. The internalization process was energy-dependent and mediated by clathrin- and caveolae-endocytosis. Co-culture studies demonstrated that the compounds exhibited selectivity towards cancer cells. Furthermore, the compounds showed non-toxicity towards cancer and/or normal cells, and visible light irradiation induced cell death, indicating their potential use as PDT agents.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Medicinal
Katarzyna Choroba, Beatriz Filipe, Anna Switlicka, Mateusz Penkala, Barbara Machura, Alina Bienko, Sandra Cordeiro, Pedro V. Baptista, Alexandra R. Fernandes
Summary: The study focused on the anticancer properties of dipicolinate-basedvanadium(IV) complexes with different diimines. These complexes showed high cytotoxic effect against HCT116-DoxR cells and induced cell death through apoptosis and autophagy pathways. They also interacted with BSA protein and did not promote tumor cell migration or angiogenesis.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Beatriz J. Coelho, Joana P. Neto, Barbara Sieira, Andre T. Moura, Elvira Fortunato, Rodrigo Martins, Pedro V. Baptista, Rui Igreja, Hugo Aguas
Summary: Microfluidic-based platforms are powerful tools for chemical and biological assays. The fusion of different microfluidic technologies has the potential to overcome limitations and enhance their strengths.
Article
Polymer Science
Beatriz J. Coelho, Joana V. Pinto, Jorge Martins, Ana Rovisco, Pedro Barquinha, Elvira Fortunato, Pedro V. Baptista, Rodrigo Martins, Rui Igreja
Summary: Poly(p-xylylene) derivatives, also known as Parylenes, have been extensively studied for their thermal, structural, and electrical properties. This article explores the application of Parylene C in various electronic devices, including transistors, capacitors, and digital microfluidic devices. The results show that Parylene C can be used as a dielectric, substrate, and encapsulation layer in transistors, producing high performance devices with steep transfer curves, low gate leak currents, and good mobilities. The study also demonstrates the functionality of Parylene C in MIM structures and its capability to enable faster droplet motion and nucleic acid amplification reactions in DMF devices.
Article
Pharmacology & Pharmacy
Catarina Roma-Rodrigues, Alexandra R. Fernandes, Pedro V. Baptista
Summary: Neoangiogenesis is associated with poor prognosis in cancer, including chronic myeloid leukemia (CML). The small GTP-binding protein Rab11a plays a crucial role in the neoangiogenic process in CML patients, by controlling exosome secretion and regulating vascular endothelial factor receptors. By downregulating Rab11a mRNA in CML cells, the angiogenic potential of exosomes can be reduced, offering a potential therapeutic approach for inhibiting tumor angiogenesis.
Editorial Material
Public, Environmental & Occupational Health
Paulo Ferrinho, Miguel Viveiros, Ines Fronteira
Review
Biochemistry & Molecular Biology
Oscar A. Lenis Rojas, Sandra Cordeiro, Pedro V. Baptista, Alexandra R. Fernandes
Summary: The ruthenium arene fragment is a valuable source for designing anticancer drugs, and the co-ligand plays a crucial role in obtaining effective anticancer complexes. N-heterocyclic carbenes (NHCs), despite their versatility and ability to stabilize metal ions, have not been extensively explored. However, recent advancements have been made in the development of NHC ruthenium arene complexes as potent anticancer agents.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
