The stem of Indian borage (Plectranthus amboinicus) was found to be an antioxidant rich fraction as evaluated by in vitro models such as DPPH free radical scavenging, reducing power assay, superoxide anion radical scavenging, and total antioxidant capacity. The extract also exhibited antiplatelet aggregation ability, antibacterial activity, and antiproliferative effect against cancer cell lines: Caco-2, HCT-15, and MCF-7. Phytochemical evaluation of the extract revealed the occurrence of total phenolics (49.91mg GAE/g extract), total flavonoids (26.6mg RE/g extract), and condensed tannins (0.7mg TAE/g extract). Among the major phenolics, rosmarinic acid (6.160mg/g extract) was predominant, followed by caffeic acid (0.770mg/g extract), rutin (0.324mg/g extract), gallic acid (0.260mg/g extract), quercetin (0.15mg/g extract), and p-coumaric acid (0.104mg/g extract). The appreciable biological activity and presence of biomolecules in the methanolic extract of stem indicate its potential application as functional food ingredients and nutraceuticals.
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