期刊
JOURNAL OF THE AMERICAN HEART ASSOCIATION
卷 2, 期 5, 页码 -出版社
WILEY
DOI: 10.1161/JAHA.113.000391
关键词
atherosclerosis; CD4(+)LAP(+) Tregs; TGF-beta; tolerogenic dendritic cells; TSLP
资金
- National Natural Science Foundations of China [81070237, 81270354, 81160045]
Background-Thymic stromal lymphopoietin (TSLP) is a cytokine with multiple effects on the body. For one thing, TSLP induces Th2 immunoreaction and facilitates allergic reaction; for another, it promotes the differentiation of naturally occurring CD4(+)CD25(+)Foxp3(+) regulatory T cells (nTregs) and maintains immune tolerance. However, the exact role of TSLP in atherosclerosis remains unknown. Methods and Results-In vitro, we examined the phenotype of TSLP-conditioned bone marrow dendritic cells (TSLP-DCs) of apolipoprotein E-deficient (ApoE(-/-)) mice and their capacity to induce the differentiation of Tregs. Our results indicated that TSLP-DCs obtained the characteristics of tolerogenic dendritic cells and increased a generation of CD4(+) latency-associated peptide (LAP)(+) Tregs and nTregs when cocultured with naive T cells. In addition, the functional relevance of TSLP and TSLP-DCs in the development of atherosclerosis was also determined. Interestingly, we found that TSLP was almost absent in cardiovascular tissue of ApoE(-/-) mice, and TSLP administration increased the levels of antioxidized low-density lipoprotein IgM and IgG(1), but decreased the levels of IgG(2a) in plasma. Furthermore, mice treated with TSLP and TSLP-DCs developed significantly fewer (32.6% and 28.2%, respectively) atherosclerotic plaques in the aortic root compared with controls, along with increased numbers of CD4(+)LAP(+) Tregs and nTregs in the spleen and decreased inflammation in the aorta, which could be abrogated by anti-TGF-beta antibody. Conclusions-Our results revealed a protective role for TSLP in atherosclerosis that is possibly mediated by reestablishing a tolerogenic immune response, which may represent a novel possibility for treatment or prevention of atherosclerosis.
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