Article
Biotechnology & Applied Microbiology
Thanawat Boontip, Rungaroon Waditee-Sirisattha, Kohsuke Honda, Suchada Chanprateep Napathorn
Summary: This study aimed to increase PHB production by enhancing the expression of a PHB biosynthesis operon derived from alkaline-tolerant strain C. necator A-04. Through careful optimization, soluble phaC(A-04) protein was successfully expressed using a fusion protein with TF under a cold shock promoter, leading to high PHB productivity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ilhan Cem Duru, Anne Ylinen, Sergei Belanov, Alan Avila Pulido, Lars Paulin, Petri Auvinen
Summary: This study sheds new light on the cold- and heat-shock response of psychrotrophic LAB. Candidate genes identified through gene network inference analysis could serve as targets for future research.
Article
Microbiology
Anna Maria Giuliodori, Riccardo Belardinelli, Melodie Duval, Raffaella Garofalo, Emma Schenckbecher, Vasili Hauryliuk, Eric Ennifar, Stefano Marzi
Summary: Escherichia coli CspA is an RNA binding protein that stimulates translation, including its own, during cold-shock. It specifically promotes the translation of cspA mRNA at low temperature by interacting with the folded conformation less accessible to the ribosome without inducing large structural rearrangements. This interaction allows the progression of ribosomes during the transition from translation initiation to elongation, and a similar mechanism may be responsible for CspA-dependent translation stimulation observed with other probed mRNAs.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Fan Jiang, Guofeng Chang, Zhenzhen Li, Mostafa Abouzaid, Xiaoyong Du, J. Joe Hull, Weihua Ma, Yongjun Lin
Summary: This study used bioinformatics approaches to evaluate the transcript levels of heat shock protein network in Chilo suppressalis under cold-induced stress. It identified highly cold-inducible proteins in C. suppressalis and found differences in transcriptional regulation between cold-acclimation and short-term cold-shock. The activation of HSP/co-chaperone network in overwintering larvae depends on their diapause state, and cold acclimation can enhance their cold tolerance.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Agriculture, Dairy & Animal Science
Ge Xue, Jingwen Yin, Ning Zhao, Yanhong Liu, Yajie Fu, Runxiang Zhang, Jun Bao, Jianhong Li
Summary: The study showed that intermittent mild cold stimulation (IMCS) can enhance the immune function of spleens and the adaptability to acute cold stress (ACS) in broilers. Among the treatments, CS5 demonstrated the most optimal results.
Article
Biotechnology & Applied Microbiology
Jingcong Liu, Shiyuan Wu, Lifang Feng, Yichu Wu, Junli Zhu
Summary: The aim of this study was to investigate the biofilm forming characteristics and stress resistance of psychrotrophic Pseudomonas at low temperatures. The results showed that the biofilm biomass of these bacteria at 4 degrees Celsius was significantly higher than at higher temperatures, and low temperature induced higher secretion of EPS. Additionally, the mature biofilms formed at 4 degrees Celsius exhibited enhanced resistance to thermal and chemical treatments. These findings suggest that the large secretion and protection of extracellular matrix at low temperatures are associated with the formation of mature biofilms and their stress resistance.
Review
Microbiology
Mansi Chauhan, Ayushi Kimothi, Avinash Sharma, Anita Pandey
Summary: The cold adapted microorganisms, psychrophiles/psychrotolerants, undergo cellular and biochemical modifications to cope with low temperature stress. These microorganisms play a crucial role in various ecological processes in cold environments. The functional capabilities of microorganisms in cold environments are determined by their strategies in cell metabolism, physicochemical constraints, and stress factors. Pseudomonas spp., a predominant group of microorganisms in cold environments, have been extensively studied for their plant growth promotion, biocontrol abilities, and biotechnological applications. This review summarizes the functional versatility of Pseudomonas, with a focus on their peculiarities and ecological and biotechnological applications.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Srijana Mukhia, Anil Kumar, Poonam Kumari, Rakshak Kumar
Summary: This study isolates cold-adapted bacteria with plant growth-promoting properties from a glacier ecosystem in the Sikkim Himalaya, suggesting their potential use in developing cold-active biofertilizers for mountain agriculture.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Microbiology
Changjie Bao, Muzi Li, Xuhui Zhao, Jia Shi, Yehui Liu, Na Zhang, Yuqi Zhou, Jie Ma, Guang Chen, Sitong Zhang, Huan Chen
Summary: The psychrotroph Pseudomonas fragi D12, a cold-tolerant bacterium, was isolated from tundra soil in the alpine zone of Changbai Mountain. Whole-genome sequencing, comparative genomics, and transcriptome analysis revealed 124 potential cold adaptation genes, including 19 unique genes. Three unique genes associated with pili protein were significantly upregulated at low temperatures, possibly contributing to the strong cold adaptability of P. fragi D12. The bacterium exhibited different cold adaptation mechanisms depending on the temperature change.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Mehdi Zarei, Sahar Elmi Anvari, Siavash Maktabi, Per Erik Joakim Saris, Amin Yousefvand
Summary: This study identified proteolytic, psychrotrophic bacteria in cold raw milk and quantified their proteolytic activity and biofilm-forming capacity. The results showed different levels of proteolytic activity among the identified isolates, with higher activity at 25°C compared to 7°C. The study also found significant variation in the biofilm-forming capacity of the bacteria.
Article
Biochemistry & Molecular Biology
Arancha Catalan-Moreno, Marta Cela, Pilar Menendez-Gil, Naiara Irurzun, Carlos J. Caballero, Isabelle Caldelari, Alejandro Toledo-Arana
Summary: The production of cold shock proteins CspB and CspC from Staphylococcus aureus is controlled by two paralogous RNA thermoswitches, which adopt alternative RNA structures at different temperatures, affecting the translation process. Simultaneous deletion of these genes or their RNA thermoswitches significantly decreases the growth rate of the bacteria at lower temperatures.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Engineering, Environmental
Yue Wang, Genji Yang, Bowen Wu, Yaozu Mi, Yang Zhou, Tian Ma, Hailian Zang, Chuanwu Xi, Cheng Yi, Chunyan Li
Summary: This study demonstrates the potential of psychrotrophic microorganisms, particularly Raoultella terrigena HC6, for simultaneous bioremediation and bioconversion of papermaking wastewater in cold regions. The HC6 strain exhibited high enzymatic activities for lignocellulose deconstruction at 15 degrees C, facilitated by the cold-inducible cold shock protein A (CspA). The mutant strain HC6-cspA overexpressing the cspA gene showed great performance in removing cellulose, hemicellulose, lignin, COD, and NO3- -N, while also producing 2,3-butanediol as a valuable byproduct.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Review
Biochemistry & Molecular Biology
Amit Chaudhary, Pankaj Kumar Chaurasia, Sandeep Kushwaha, Pallavi Chauhan, Aakash Chawade, Ashutosh Mani
Summary: Cold shock proteins are a conserved family of proteins that play important roles in low-temperature stress response and nucleic acid binding in bacteria. They are involved in various cellular and metabolic processes in prokaryotes and have been found to be crucial in the progression of cancer and Alzheimer's disease in humans.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Entomology
Asli Dageri, Mohammed Lengichow Kadir, Nurper Guz, Ayhan Ogreten, Muhammad Arshad
Summary: In this study, two cold-regulated genes, TgAFP and TgCSDP, were identified in T. granarium. The expression of these genes in response to temperature and cold stress was examined. The results suggest that these genes play crucial roles in the cold survival and adaptation of T. granarium.
JOURNAL OF STORED PRODUCTS RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Jinxiu Xu, Qiuying Li, Jingyang Zhang, Xuepeng Li, Tong Sun
Summary: Pseudomonas fluorescens PF08 genome contains five typical cold shock proteins (CSPs) which are highly conserved and play an important role in bacterial cold adaptation. These CSPs are small hydrophilic acidic proteins located in the cytoplasm, and they interact with proteins involved in virulence, stress responses (including to low temperature), cell growth, ribosome assembly, and RNA degradation. The secondary and tertiary structures of PfCSPs are characteristic of the CSP family, and they are predicted to have ligand binding sites and high functional similarity to CspD from Escherichia coli and Salmonella Typhimurium.
JOURNAL OF FOOD PROTECTION
(2021)
Article
Biotechnology & Applied Microbiology
Pankaj Kumar Mishra, Shekhar Chandra Bisht, Pooja Ruwari, Avupati Raja Naga Satya Subbanna, Jaideep Kumar Bisht, Jagdish Chandra Bhatt, Hari Shankar Gupta
ANNALS OF MICROBIOLOGY
(2017)
Article
Biotechnology & Applied Microbiology
Pankaj K. Mishra, Smita Mishra, Govindan Selvakumar, Shekhar C. Bisht, Jaideep K. Bisht, Samaresh Kundu, Hari Shankar Gupta
ANNALS OF MICROBIOLOGY
(2008)
Article
Microbiology
Pankaj Kumar Mishra, Shekhar Chandra Bisht, Pooja Ruwari, Govindan Selvakumar, Gopal Krishna Joshi, Jaideep Kumar Bisht, Jagdish Chandra Bhatt, Hari Shankar Gupta
ARCHIVES OF MICROBIOLOGY
(2011)
Article
Microbiology
Shekhar Chandra Bisht, Pankaj Kumar Mishra, Gopal Kishna Joshi
ARCHIVES OF MICROBIOLOGY
(2013)
Article
Ecology
Pankaj K. Mishra, Shekhar C. Bisht, Pooja Ruwari, Gopal K. Joshi, G. Singh, Jaideep K. Bisht, J. C. Bhatt
EUROPEAN JOURNAL OF SOIL BIOLOGY
(2011)
Article
Ecology
Tanuja, Shekhar C. Bisht, Pankaj K. Mishra
EUROPEAN JOURNAL OF SOIL BIOLOGY
(2013)
Article
Plant Sciences
Pankaj K. Mishra, Shekhar C. Bisht, Smita Mishra, G. Selvakumar, J. K. Bisht, H. S. Gupta
JOURNAL OF PLANT NUTRITION
(2012)
Article
Multidisciplinary Sciences
M. Y. Areeshi, Raju K. Mandal, Aditya K. Panda, Shekhar C. Bisht, Shafiul Haque
Review
Infectious Diseases
Mohammed Yahya Areeshi, Shekhar Chandra Bisht, Raju Kumar Mandal, Shafiul Haque
JOURNAL OF INFECTION IN DEVELOPING COUNTRIES
(2014)
Article
Agronomy
Pankaj Kumar Mishra, Shekhar Chandra Bisht, Krishnan Jeevanandan, Sanjay Kumar, Jaideep Kumar Bisht, Jagdish Chandra Bhatt
ARCHIVES OF AGRONOMY AND SOIL SCIENCE
(2014)
Article
Multidisciplinary Sciences
Shekhar Chandra Bisht, Gopal Kishna Joshi, Shafiul Haque, Pankaj Kumar Mishra
Review
Oncology
Shafiul Hague, Dinesh K. Yadav, Shekhar C. Bisht, Neelam Yadav, Vineeta Singh, Kashyap Kumar Dubey, Arshad Jawed, Mohd Wahid, Sajad Ahmad Dar
JOURNAL OF CHEMOTHERAPY
(2019)
Article
Biology
Pankaj K. Mishra, Smita Mishra, Shekhar C. Bisht, G. Selvakumar, S. Kundu, J. K. Bisht, Hari Shankar Gupta
BIOLOGICAL RESEARCH
(2009)