Article
Chemistry, Analytical
Isabel Meister, Pei Zhang, Anirban Sinha, C. Magnus Skold, Asa M. Wheelock, Takashi Izumi, Romanas Chaleckis, Craig E. Wheelock
Summary: This study developed a method for measuring specific gravity in urine using a refractive index detector (RID) in a 96-well-plate format, providing a new solution for metabolomic research on this noninvasive biofluid. By developing an automated LC-MS workflow and utilizing multiple technical internal standards to monitor data quality, over 540 urinary metabolites were successfully identified.
ANALYTICAL CHEMISTRY
(2021)
Article
Food Science & Technology
Thammaporn Junsai, Saranya Poapolathep, Samak Sutjarit, Mario Giorgi, Zhaowei Zhang, Antonio Francesco Logrieco, Peiwu Li, Amnart Poapolathep
Summary: This study investigated the occurrence of multi-mycotoxins in six types of vegetable oils available in Thailand, revealing variations in mycotoxin contamination among different oil samples. The levels of analyzed mycotoxins were found to be below regulatory limits, indicating a low health risk to consumers.
Article
Biochemical Research Methods
Cheryl Turner, Helga Refsum, Nasser E. Bastani
Summary: In this study, an analytical method based on liquid chromatography with electrospray ionization coupled to tandem mass spectrometry detection was developed for accurate quantification of various amino acids in human plasma. The method showed excellent linearity, accuracy, precision, sensitivity, and selectivity.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Food Science & Technology
Sufang Fan, Junmei Ma, Meirong Cao, Juan Wang, Leilei Zhang, Yan Zhang, Qiang Li, Jia Chen
Summary: A new liquid chromatography tandem mass spectrometry method was developed for the rapid and accurate determination of 15 pesticide residues in Chinese cabbage and cucumber samples. After QuEChERS extraction and online turbulent flow chromatography purification, the recoveries and matrix effects of pesticide residues were within acceptable ranges.
FOOD SCIENCE AND HUMAN WELLNESS
(2021)
Article
Chemistry, Applied
Yuanjia Chen, Zhuofeng Ke, Zhenlin Xu, Weilong Huang, Yuanming Sun, Hongtao Lei, Xiaoqun Wei
Summary: A rapid, low-cost, and selective liquid chromatography tandem mass spectrometry method was developed and validated for simultaneous determination of maneb group residues in fruit. The validated method showed sensitive quantification limits, steady recovery, and excellent precision. This method was successfully applied to analyze fruit samples and achieved satisfactory results.
Article
Biochemistry & Molecular Biology
Nicolas Sambiagio, Jean-Jacques Sauvain, Aurelie Berthet, Reto Auer, Anna Schoeni, Nancy B. Hopf
Summary: Human biomonitoring of oxidative stress often relies on urinary biomarkers such as 8-oxodG and 8-isoprostane, but inconsistent levels reported in scientific literature can be attributed to the various analytical methods used. A newly developed UPLC-MS/MS method allows for simultaneous quantification of both biomarkers with high accuracy and precision. This method shows promise for biomonitoring and epidemiological studies focusing on oxidative damage.
Article
Food Science & Technology
Xin Guan, Yuchao Feng, Decheng Suo, Zhiming Xiao, Shi Wang, Ying Liang, Xia Fan
Summary: In this study, a method for detecting 11 mycotoxins in maize was established using multiple-impurity adsorption purification (MIA) technologies and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The validated method was suitable for simultaneous determination of multiple mycotoxins in maize.
Article
Chemistry, Analytical
Alex Gomez-Gomez, MohammadA Sabbaghi, Noemi Haro, Joan Albanell, Silvia Menendez, Monica Gonzalez, Gabriel Gil-Gomez, Ana Rovira, Oscar J. Pozo
Summary: Formalin-fixed paraffin-embedded (FFPE) tissues are crucial in cancer research, but research on the detection of small molecules in FFPE samples is limited. This study aimed to explore the potential of targeted metabolomics in FFPE specimens and developed a LC-MS/MS method for the quantification of acidic metabolites. The method showed accurate and precise quantification of 40 metabolites in FFPE samples, with implications for cancer research.
Article
Chemistry, Applied
Peng Sun, Zhao Yu, Chao Wang, Shuo Li
Summary: In this study, the content of eight sterols in rapeseed, including cholesterol, cholestanol, brassicasterol, ergosterol, campesterol, stigmasterol, 8-sitosterol, and stigmastanol, was determined using pressurized liquid extraction coupled with gas chromatography-mass spectrometry. The results showed a good linear relationship between the peak area and the corresponding mass concentration of the eight sterols. The developed method was efficient, simple, and accurate, suitable for the qualitative and quantitative analysis of sterols in rapeseed.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Chemistry, Analytical
Anran Sheng, Qingqing Chen, Mengqi Yu, Ruiqi Xiao, Tianji Zhang, Zhiyu Wang, Robert J. Linhardt, Xiaojun Sun, Lan Jin, Lianli Chi
Summary: Glycosaminoglycans (GAGs) play a role in the treatment of many human diseases, especially in thrombosis, due to their anticoagulant activity. Novel electrophoresis-mass spectrometry methods have been developed to directly elucidate the structures of larger GAG chains with more biological importance, aiding in understanding the structure-activity relationship and quality control of GAG drugs.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Sophie Catanese, Raoul K. Khanna, Antoine Lefevre, Hugo Alarcan, Pierre-Jean Pisella, Patrick Emond, Helene Blasco
Summary: To apply metabo-lipidomics in ophthalmological and systemic diseases, it is necessary to standardize the preanalytical and analytical steps in human tears. This study compared different methods for each step of the workflow and found that methanol/water extraction provided the best results for metabolomics, while Folch extraction was superior for lipidomics. The use of microcapillary glass tube for sampling was superior to Schirmer strip for metabolomics. A volume of 5 mu L provided satisfactory metabo-lipidomic coverage. These findings will contribute to improving the metabo-lipidomic workflow in human tears for the identification of new biomarkers.
Article
Chemistry, Analytical
A. Arrizabalaga-Larranaga, S. Epigmenio-Chamu, F. J. Santos, E. Moyano
Summary: This paper describes the development of a rapid and sensitive method for detecting banned dyes in spice samples using UHPLC-MS/MS technology combined with three atmospheric pressure ionization sources. The proposed method demonstrated good performance in sample analysis, with low detection limits, good precision, and accurate quantitation.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Patrik Matlak, Hana Brozmanova, Pavel Sistik, Ivana Kacirova, Pavel Hradilek, Milan Grundmann
Summary: This study aimed to develop a LC-MS/MS method for quantifying natalizumab in human serum and cerebrospinal fluid (CSF) and applying it to clinical settings. By analyzing specific peptide sequences of natalizumab, the concentration was successfully determined. The method met all accuracy and precision acceptance criteria according to the EMA guideline and is suitable for clinical applications.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Review
Chemistry, Analytical
A. Fernez Asensio, E. Alvarez-Gonzalez, A. Rodriguez, L. M. Sierra, E. Blanco-Gonzalez
Summary: Dysregulation of TCA cycle enzymes leads to abnormal accumulation of oncometabolites, which contribute to cancer formation and progression. GC-MS and LC-MS are powerful analytical tools for accurate identification and determination of these metabolites.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Applied
Xuan Zhang, Changling Fang, Dongmei Huang, Guangxin Yang, Yunyu Tang, Yongfu Shi, Cong Kong, Pei Cao, Youqiong Cai
Summary: A method for the determination of 8 biogenic amines in aquatic products and their derived products was established by HPLC-MS/MS without derivatization. The method achieved good linearity, higher sensitivity, and demonstrated average recoveries in the range of 70.9% to 113.1% with low relative standard deviations. It was proven suitable for the detection of BAs in aquatic products and their products.