Article
Plant Sciences
Zheng Qin, Zi-Zhen Liang, Ya-Nan Wu, Xue-Qing Zhou, Meng Xu, Li-Wen Jiang, Sha Li, Yan Zhang
Summary: Ovules are the female reproductive organs of angiosperms, consisting of integuments surrounding embryo sacs. Communication between integument growth and embryo sac development is crucial for synchronization. This study reveals that symplastic signals through plasmodesmata (PDs) in integuments play a critical role in the development of female gametophytes. Interference with PD biogenesis leads to defective ovules that fail to attract pollen tubes and result in fertilization failure.
Article
Biochemistry & Molecular Biology
Jie Liu, Yao Liu, Shuang Wang, Yongqi Cui, Dawei Yan
Summary: This study reveals that heat stress induces callose accumulation at the plasmodesmata connecting sieve element and phloem pole pericycle, resulting in decreased root meristem size and inhibition of phloem unloading. It further identifies CALLOSE SYNTHASE 8 (CalS8) as a negative regulator of thermotolerance in Arabidopsis roots by decreasing plasmodesmal callose deposition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Nicolas Carlotto, Gabriel Robles-Luna, Alexander Nedo, Xu Wang, Alejandra Attorresi, Jeffrey Caplan, Jung Y. Lee, Ken Kobayashi
Summary: Defects in chloroplast RNA metabolism mediate plasmodesmata gating by suppressing plasmodesmata callose deposition.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Plant Sciences
Luigi Parrotta, Claudia Faleri, Cecilia Del Casino, Lavinia Mareri, Iris Aloisi, Gea Guerriero, Jean-Francois Hausman, Stefano Del Duca, Giampiero Cai
Summary: This article investigates the association between callose synthase and cytoskeleton in the pollen tube. The study confirms this association and demonstrates colocalization using immunogold labeling. The results suggest that callose synthase interacts with microtubules and exerts its activity in cooperation with the microtubular cytoskeleton.
PLANT CELL REPORTS
(2022)
Review
Plant Sciences
Jie Liu, Lin Zhang, Dawei Yan
Summary: Plasmodesmata play crucial roles in mediating symplastic communication in plants, facilitating cell-to-cell trafficking of molecules essential for plant development and stress responses. However, they can also be exploited by pathogens to infect hosts, prompting plants to develop defense mechanisms to regulate PD permeability. By understanding the interactions between pathogens and plants through PD, strategies for enhancing disease resistance can be proposed based on current knowledge.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Shuai Fang, Xiaoguang Shang, Qingfei He, Weixi Li, Xiaohui Song, Baohong Zhang, Wangzhen Guo
Summary: A cell wall-localized β-1,3-glucanase, GhGLU18, enhances polysaccharide metabolism and cell wall synthesis to promote fiber cell elongation and thickening in cotton. GhGLU18 specifically expressed in fibers and plays a role in fiber cell wall development.
Article
Plant Sciences
Lingmin Cai, Jie Liu, Shuchang Wang, Zihui Gong, Siyu Yang, Fengyuan Xu, Zhongyuan Hu, Mingfang Zhang, Jinghua Yang
Summary: The WPRb gene is associated with CGMMV resistance in watermelon by affecting the permeability of plasmodesmata. Editing of WPRb confers greater tolerance to CGMMV. Our findings enable us to genetically control CGMMV resistance in watermelon.
Review
Plant Sciences
Ning Li, Zeng Lin, Peiyao Yu, Yanling Zeng, Shenxiu Du, Li-Jun Huang
Summary: Callose, a linear polysaccharide, is synthesized in plant cell walls and is composed of beta-1,3-linked glucose residues with beta-1,6-linked branches. It is found in almost all plant tissues and is involved in various stages of plant growth and development. Callose is induced by heavy metal treatment, pathogen invasion, and mechanical wounding, and is synthesized by callose synthases on the cell membrane. Recent research on plant callose and its synthetizing enzymes sheds light on its important and versatile role in plant life activities.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Behnaz Saatian, Susanne E. E. Kohalmi, Yuhai Cui
Summary: Cell-to-cell communication is crucial for plant development and response to stress. Proteins called PDLPs and GSLs are important for regulating plasmodesmal permeability and intercellular communication. It has been observed that PDLP5 can limit plasmodesmal permeability through inducing callose accumulation, but the mechanism behind this process is still unclear.
PLANT SIGNALING & BEHAVIOR
(2023)
Review
Plant Sciences
Liam German, Richa Yeshvekar, Yoselin Benitez-Alfonso
Summary: Cell walls are critical for plant growth and development, providing support and protection. Callose, a type of glucan, accumulates in specialized cell wall microdomains, including plasmodesmata. It regulates important biological processes and affects the transport of signaling proteins and RNA molecules. The mechanisms controlling callose synthesis and degradation are still unresolved. This review explores recent literature on callose metabolism and its response to mutualistic symbionts and pathogenic elicitors.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Agronomy
Ronghua Qiu, Yang Liu, Zhengzheng Cai, Jieqiong Li, Chunyan Wu, Gang Wang, Chenchen Lin, Yulin Peng, Zhanlin Deng, Weiqi Tang, Weiren Wu, Yuanlin Duan
Summary: In this study, it was found that the OsGSL2 gene plays a crucial role in rice fertility, as well as embryo differentiation and endosperm-free nucleus positioning. This reveals a distinct function of the callose synthase gene in seed initiation in cereals.
Article
Plant Sciences
Junqing Wu, Wenru Sun, Chao Sun, Chunmiao Xu, Shuang Li, Pengxue Li, Huimin Xu, Danyang Zhu, Meng Li, Liling Yang, Jinbo Wei, Aya Hanzawa, Sumaiya Jannat Tapati, Reiko Uenoyama, Masao Miyazaki, Abidur Rahman, Shuang Wu
Summary: Fruit malformation is a widespread issue in fruit production, causing significant economic losses. Although farmers have long observed the association between chilling temperature before blooming and malformed fruits, the molecular mechanism behind this phenomenon remains unclear. In this study, we investigated how tomato fruit development responded to cold stress and found that short-term cold stress led to increased callose accumulation in both shoot apical and floral meristems. This caused symplastic isolation and altered intercellular movement of WUS, resulting in expanded stem cell population and malformed fruits. Our findings suggest that the callose dynamics during short-term cold stress involve the interplay of abscisic acid and gibberellin, similar to bud dormancy mechanisms. This study provides new insights into the regulation of fruit malformation under cold stress.
Review
Biochemistry & Molecular Biology
Youssef Chebli, Amir J. Bidhendi, Karuna Kapoor, Anja Geitmann
Summary: The plant cell wall serves as an important extracellular matrix that envelops cells, maintains their shape and structure, interacts with symbionts, and protects against external stresses. The assembly of this matrix is regulated by the cytoskeleton, which also plays a key role in perceiving mechanical cues and mediating intracellular responses related to cell wall structure changes. Delivery processes of cell wall precursors and their structural continuity are crucial for cell wall assembly, with various morphogenetic processes relying on cell wall assembly as a critical element.
Review
Multidisciplinary Sciences
Bin Wang, Mebeaselassie Andargie, Ruiqiu Fang
Summary: This review compares and analyzes the regulation of cellulose and callose synthesis in plant cell walls, with a particular emphasis on the future research direction of callose synthesis.
Article
Plant Sciences
Zhongpeng Li, Haris Variz, Yani Chen, Su-Ling Liu, Kyaw Aung
Summary: Pathogenic microorganisms deliver protein effectors to suppress host immune responses, recent findings show phytopathogens manipulating plant cell-to-cell communication channels to promote diseases. Several pathogen effectors regulate these communication channels in host cells, allowing for intercellular movement. Some effectors can move between infected and neighboring plant cells through these channels, though it is unclear if bacterial effectors can do the same.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Forestry
Ana I. Ribeiro-Barros, Katharina Pawlowski, Jose C. Ramalho
Summary: This mini-review provides an overview of salt-stress tolerance in the Casuarinaceae family, focusing on the contribution of root-nodule and arbuscular mycorrhizal symbioses, as well as related eco-physiological and molecular changes.
JOURNAL OF FOREST RESEARCH
(2022)
Article
Plant Sciences
Alexey S. Kiryushkin, Elena L. Ilina, Elizaveta D. Guseva, Katharina Pawlowski, Kirill N. Demchenko
Summary: CRISPR/Cas-mediated genome editing and hairy root transformation are fast and effective methods for studying plant gene function. This review outlines the recent advances achieved by combining these techniques and discusses the origins of hairy root transformation strains, components of CRISPR/Cas vectors, and the application of CRISPR/Cas genome editing in hairy roots. Additionally, a method for modifying a known vector is described.
Review
Plant Sciences
E. Tyutereva, A. Murtuzova, O. V. Voitsekhovskaja
Summary: In plant cells, the homeostatic control of energy balance involves the production and recycling of adenylates with macroergic bonds, ATP and ADP. Autophagy plays a crucial role in energy metabolism by actively degrading unwanted or damaged cell components and macromolecules. SnRK1 and TOR are central protein kinases that regulate the switch between anabolic and catabolic pathways. SnRK1 induces autophagy in response to a decline in cellular energy supply, while TOR promotes energy-demanding metabolic reactions and suppresses autophagy.
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
(2022)
Review
Biophysics
Marina Kozuleva, Boris Ivanov, Olga Voitsekhovskaja
Summary: The 'Regulation of Photosynthesis' session was a significant event at the Congress, featuring cutting-edge research topics in modern photosynthesis. The reports discussed various areas such as electron transport, the novel role of carbonic anhydrase in pH regulation, retrograde signaling, and non-invasive techniques for measuring photosynthetic response.
BIOPHYSICAL REVIEWS
(2022)
Article
Biochemistry & Molecular Biology
Julia Shumilina, Alexey S. Kiryushkin, Nadezhda Frolova, Valeria Mashkina, Elena L. Ilina, Vera A. Puchkova, Katerina Danko, Svetlana Silinskaya, Evgeny B. Serebryakov, Alena Soboleva, Tatiana Bilova, Anastasia Orlova, Elizaveta D. Guseva, Egor Repkin, Katharina Pawlowski, Andrej Frolov, Kirill N. Demchenko
Summary: The main role of RALF small signaling peptides is to control the alkalization of the apoplast for nutrient absorption improvement. However, the specific function of individual RALF peptides such as RALF34 is still unknown. In this study, we used cucumber transgenic hairy roots overexpressing CsRALF34 to investigate its role in the regulatory pathway. The results showed that CsRALF34 overexpression inhibited root growth and regulated cell proliferation, specifically blocking the G2/M transition in cucumber roots. We propose that CsRALF34 modulates ROS homeostasis and triggers controlled production of hydroxyl radicals, possibly associated with intracellular signal transduction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Alexey S. Kiryushkin, Elena L. Ilina, Elizaveta D. Guseva, Katharina Pawlowski, Kirill N. Demchenko
Summary: This study aimed to understand the signaling induced by RALF34 in cucumber. The expression of CsRALF34 was observed in all plant organs, and unlike AtRALF34, its expression was not regulated by auxin. CsTHESEUS1 showed constitutive expression in cucumber root tissues. The results suggest that CsRALF34 acts in a non-cell-autonomous manner and is not involved in lateral root initiation in cucumber.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yingting Xu, Yingchao Xu, Zhengwan Huang, Yucai Luo, Ruanling Gao, Jinghua Xue, Changli Lin, Katharina Pawlowski, Zhongyu Zhou, Xiaoyi Wei
Summary: In this study, three undescribed 3-pentanol glycosides were isolated and identified from the root nodules of Alnus cremastogyne. It was found that 3-pentanol glycosides were abundantly distributed in root nodules, but in low/zero levels in stems, roots, leaves, and fruits. The treatment of A. cremastogyne plants with root nodule suspension resulted in the emission of 3-pentanol. This study provides valuable information on specialized metabolites in the actinorhizal host and the signal exchange in the actinorhizal symbiosis between A. cremastogyne and Frankia.
Article
Food Science & Technology
Elena V. V. Tyutereva, Anna A. A. Dalinova, Kirill N. N. Demchenko, Valeriya A. A. Dmitrieva, Vsevolod R. R. Dubovik, Yuriy V. V. Lukinskiy, Galina V. V. Mitina, Olga V. V. Voitsekhovskaja, Alexander Berestetskiy
Summary: This study investigated the effects of two ten-membered lactones on different plants and found that these toxins caused leaf damage, decreased pigment content, and electrolyte leakage. Additionally, these compounds also affected mitochondrial membrane potential and cell division. The results of this study revealed the mechanisms of action of these phytotoxic macrolides.
Editorial Material
Plant Sciences
Andrea Genre, Katharina Pawlowski, Sabine Dagmar Zimmermann, Sergio Saia
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Ecology
Marina A. Romanova, Valentina V. Domashkina, Anastasiia I. Maksimova, Katharina Pawlowski, Olga V. Voitsekhovskaja
Summary: Recent advances in plant developmental genetics and transcriptomic data accumulation provide an exciting opportunity to explore the evolution of plant morphology. This study combines molecular and structural data to understand leaf origin in different plant lineages and addresses various aspects of leaf development and morphology. The data suggests that leaf initiation patterns correlate with shoot apical meristem (SAM) structure, while later stages of leaf development are not correlated with SAM structure or taxonomy. The study also discusses the expression of leaf development regulators and proposes a potential co-option of sporangium developmental program for the origin of microphylls and euphylls.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2023)