Letter
Plant Sciences
Vajiheh Safavi-Rizi
Summary: The research aims to develop genetically encoded sensors for nitric oxide bioimaging in plants.
Article
Cell Biology
Amy H. Ponsford, Thomas A. Ryan, Andrea Raimondi, Emanuele Cocucci, Susanne A. Wycislo, Florian Froehlich, Laura E. Swan, Massimiliano Stagi
Summary: Peripheral lysosomes are not less acidic compared to perinuclear lysosomes, and lysosomal pH has a certain impact on the transmission of lysosomal stress signals.
Review
Chemistry, Analytical
Cong Quang Vu, Satoshi Arai
Summary: Genetically encoded fluorescence lifetime biosensors provide a powerful tool for quantitative imaging, enabling precise measurement of cellular metabolites, molecular interactions, and dynamic cellular processes. This review gives an overview of the principles, applications, and advancements in quantitative imaging with genetically encoded fluorescence lifetime biosensors using fluorescence lifetime imaging microscopy (go-FLIM), highlighting the distinct advantages of fluorescence lifetime-based measurements.
Article
Chemistry, Analytical
Alexander S. Goryashchenko, Alexey A. Pakhomov, Anastasia Ryabova, Igor D. Romanishkin, Eugene G. Maksimov, Alexander N. Orsa, Oxana Serova, Andrey A. Mozhaev, Margarita A. Maksimova, Vladimir Martynov, Alexander G. Petrenko, Igor E. Deyev
Summary: A genetically encoded fluorescent sensor, SypHerExtra, was developed to measure extracellular media pH in a mildly alkaline range. This sensor can be used in live eukaryotic cells, along with another sensor, SypHer3s, which measures intracellular pH, for quantitative measurements using the FLIM method.
Review
Chemistry, Analytical
Yidan Mo, Huangmei Zhou, Jinming Xu, Xihang Chen, Lei Li, Sanjun Zhang
Summary: Genetically encoded biosensors based on fluorescent proteins provide a powerful tool for tracking analytes and cellular events. Fluorescence lifetime readout, which is independent of expression level and instruments, plays a vital role in accurate quantitative assessments in complex biological systems. This review provides an overview of the categorization and working mechanism of genetically encoded fluorescence lifetime biosensors, as well as their combination with fluorescence lifetime imaging technique and lifetime analysis methods, applications, and considerations for design and optimization.
Article
Plant Sciences
Rainer Waadt, Joerg Kudla, Hannes Kollist
Summary: Understanding plant physiology at the organismic scale involves analyzing biological processes down to the cellular and molecular levels. Fluorescent protein (FP) technology allows for direct monitoring of various cellular dynamics in plants, and multiplexing techniques enable simultaneous recording of multiple biological processes.
Editorial Material
Biochemical Research Methods
Justin W. Taraska
Summary: Protein-based molecular shape probes are bringing us closer to achieving a genetically encoded tagging system for cryogenic electron tomography.
Review
Biochemistry & Molecular Biology
Vera S. Ovechkina, Suren M. Zakian, Sergey P. Medvedev, Kamila R. Valetdinova
Summary: Genetically encoded fluorescent biosensors allow visualization of biological processes in real time, providing benefits such as monitoring drug action and screening at the single-cell resolution. Research aims to improve biomedical applications by exploring their potential and addressing challenges.
Review
Biochemical Research Methods
Crystian I. Massengill, Julian Day-Cooney, Tianyi Mao, Haining Zhong
Summary: cAMP is a key second messenger in biological functions, especially in regulating neuronal plasticity and excitability. Its signaling events are both cell-specific and subcellularly compartmentalized.
JOURNAL OF NEUROSCIENCE METHODS
(2021)
Article
Plant Sciences
Hortense Moreau, Isabelle Gaillard, Nadine Paris
Summary: Using genetically encoded tandems of fluorescent proteins, this study demonstrates the complex environment and pH heterogeneity within pavement cells. The newly designed acid-sensing tandems cover the pH range of 3-8 and show immediate responses to pH changes, providing insights into physiological conditions related to cell elongation and H+-ATPase activity.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Multidisciplinary Sciences
Dorothy Koveal, Paul C. Rosen, Dylan J. Meyer, Carlos Manlio Diaz-Garcia, Yongcheng Wang, Li-Heng Cai, Peter J. Chou, David A. Weitz, Gary Yellen
Summary: Combining droplet microfluidics and automated fluorescence imaging, a screening modality was developed to increase screening throughput and enable evaluation of multiple biosensor features simultaneously. This approach was used to generate a high-performance biosensor for lactate quantification.
NATURE COMMUNICATIONS
(2022)
Review
Developmental Biology
Akinobu Nakamura, Yuhei Goto, Yohei Kondo, Kazuhiro Aoki
Summary: This review provides an overview of the use of genetically encoded fluorescent biosensors for visualizing ERK activity in live cells, as well as their applications in developmental studies of model organisms. It discusses the current understanding of how ERK dynamics are encoded and decoded for cell fate decision-making.
Article
Biochemistry & Molecular Biology
Salma Saeed Khan, Yi Shen, Muhammad Qaiser Fatmi, Robert E. Campbell, Habib Bokhari
Summary: Genetically encoded biosensors based on engineered fluorescent proteins play a key role in monitoring specific ions and molecules in biological systems, with the potential to detect highly toxic arsenic ions. The development of prototype arsenic biosensors using a bacterial transcription factor scaffold opens up possibilities for improved performance in detecting As3+ in various biological and environmental systems.
Article
Biochemistry & Molecular Biology
Maryna Loewe, Sebastian Haensch, Eymen Hachani, Lutz Schmitt, Stefanie Weidtkamp-Peters, Alexej Kedrov
Summary: Biochemical processes in crowded cellular environments, such as membranes, are affected by macromolecular crowding. Researchers developed a fluorescence-based sensor to quantify macromolecular crowding at membrane interfaces, and validated its dynamics in Escherichia coli inner membrane vesicles.
Review
Chemistry, Analytical
Yidan Mo, Huangmei Zhou, Jinming Xu, Xihang Chen, Lei Li, Sanjun Zhang
Summary: Genetically encoded biosensors based on fluorescent proteins are powerful tools for tracking analytes and cellular events in living cells and organisms with high spatial and temporal resolution. Fluorescence lifetime readout provides absolute measurements, independent of biosensor expression level and instruments, making genetically encoded fluorescence lifetime biosensors vital for accurate quantitative assessments within intricate biological systems.
Article
Oncology
Chella Krishna Vadivel, Maria Gluud, Sara Torres-Rusillo, Lasse Boding, Andreas Willerslev-Olsen, Terkild B. Buus, Tea Kirkegaard Nielsen, Jenny L. Persson, Charlotte M. Bonefeld, Carsten Geisler, Thorbjorn Krejsgaard, Anja T. Fuglsang, Niels Odum, Anders Woetmann
Summary: This study demonstrates the nuclear localization of JAK3 in malignant T cells and its interactions with key nuclear proteins, suggesting a novel non-canonical role in cutaneous T cell lymphoma.
Article
Plant Sciences
Johannes Liesche, Christopher Vincent, Xiaoyu Han, Maciej Zwieniecki, Alexander Schulz, Chen Gao, Rodrigue Bravard, Sean Marker, Tomas Bohr
Summary: The study found that despite physical constraints on sugar export in long needles due to phloem anatomy, this limitation can be overcome through the grouping of transport conduits and a shift in the diurnal rhythm of sugar metabolism and export in needle tips. These mechanisms have a significant influence on leaf function and sugar transport efficiency.
Review
Genetics & Heredity
Amalie Scheel Tost, Astrid Kristensen, Lene Irene Olsen, Kristian Buhl Axelsen, Anja Thoe Fuglsang
Summary: The PSY peptide family, including PSY1 and seven homologs, plays important roles in plant development with high conservation in the active peptide domain. These peptides are found in all higher plants and mosses, potentially carrying sulfated tyrosine through post-translational modification and serving multiple functions in plant development.
Review
Biochemistry & Molecular Biology
Nanna W. Havshoi, Anja T. Fuglsang
Summary: This review evaluates the non-protein natural compounds with regulatory effects on the PM H+-ATPase, focusing on their mechanism of action and their potential as biologicals/growth regulators in future sustainable agricultural plant production.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Plant Sciences
Aart J. E. van Bel, Alexander Schulz, John W. Patrick
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Biology
Anett Steger, Maki Hayashi, Emil Wacenius Lauritzen, Klaus Herburger, Lana Shabala, Cuiwei Wang, Amalie Kofoed Bendtsen, Anton Frisgaard Norrevang, Kenneth Madriz-Ordenana, Shichao Ren, Mai Duy Luu Trinh, Hans Thordal-Christensen, Anja Thoe Fuglsang, Sergey Shabala, Jeppe Thulin osterberg, Michael Palmgren
Summary: The C-terminal autoinhibitory regulatory domain of plant PM H+-ATPases plays a crucial role in plant fitness by controlling growth and nutrient uptake, and may have been essential in the successful transition of plants from water to land.
COMMUNICATIONS BIOLOGY
(2022)
Article
Oncology
Mikkel Hoeberg, Julie Boertmann Noer, Mette Vixo Vistesen, Annette Bartels, Esben Matzen Bech, Sune Boris Nygard, Ulrik Lademann, Jan Stenvang, Siqi Liu, Anja Thoe Fuglsang, Nils Bruenner, Jose Manuel Afonso Moreira
Summary: Tissue inhibitor of metalloproteinases-1 (TIMP-1) plays a crucial role in regulating proteolytic activity and maintaining the extracellular matrix. This protein has been associated with multiple MMP-independent cytokine-like functions. Through a yeast two-hybrid screening, multiple interaction partners, including CD74, were identified, shedding light on the complex nature of TIMP-1 functions and their mechanisms.
MOLECULAR ONCOLOGY
(2023)
Article
Multidisciplinary Sciences
Deyang Xu, Niels Christian Holm Sanden, Line Lykke Hansen, Zeinu Mussa Belew, Svend Roesen Madsen, Lasse Meyer, Morten Egevang Jorgensen, Pascal Hunziker, Dorottya Veres, Christoph Crocoll, Alexander Schulz, Hussam Hassan Nour-Eldin, Barbara Ann Halkier
Summary: Plant membrane transporters control metabolite distribution, contributing to key agronomic traits. Mutations in importers can prevent the accumulation of anti-nutritional factors in edible parts, but may alter the distribution pattern within the plant. Engineering of exporters can prevent these changes while still improving the nutritional quality of the seeds.
Article
Plant Sciences
Chen Gao, Sean J. Marker, Carsten Gundlach, Henning F. Poulsen, Tomas Bohr, Alexander Schulz
Summary: This study investigates the transportation pathway of photoassimilates and nutrients in conifer needles and reveals the functional 3D structure of the transfusion tissue in the bundle sheath. The results highlight the crucial role of the bundle sheath in nutrient and assimilate exchange.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Marc Fradera-Soler, Jozef Mravec, Alexander Schulz, Rafael Taboryski, Bodil Jorgensen, Olwen M. Grace
Summary: Hydathode-mediated foliar water uptake (FWU) has been observed in the leaf-succulent genus Crassula, a diverse group of plants in southern Africa. This FWU ability is not restricted to arid environments and is independent of leaf surface wettability. It is an adaptation that allows Crassula species to harvest moisture from the atmosphere.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Chemistry, Multidisciplinary
Peter Klemmed Bjork, Nicolai Tidemand Johansen, Nanna Weise Havshoi, Silas Anselm Rasmussen, Johan orskov Ipsen, Thomas Isbrandt, Thomas Ostenfeld Larsen, Anja Thoe Fuglsang
Summary: The specific components trichorzin PA (tPA) II and tPA VI in Trichoderma spp. fungal extracts can enhance the activity of H+-ATPase on plant membranes, thereby promoting plant growth. These components belong to the class of peptaibols, which can form indiscriminate ion channels and exhibit pesticidal activity. Peptaibol-mediated H+-ATPase activation is a concentration-dependent balance.
Article
Plant Sciences
Niels Christian Sanden, Alexander Schulz
Summary: The transportation of photoassimilates in vascular plants is facilitated by the phloem, which involves mature sieve elements that lose cellular contents and provide a low resistance path. The protein machinery in these sieve elements is vital for maintaining flow, transmitting signals, and defending against pests. However, our knowledge of this protein population is limited. In this study, co-expression analysis and gene clusters from transcriptomic studies were used to identify potential sieve element proteins. The results provide valuable resources for studying uncharacterized genes in mature sieve elements.
PHYSIOLOGIA PLANTARUM
(2022)
Correction
Plant Sciences
Anja T. Fuglsang, Michael Palmgren
Article
Forestry
Xiaoyu Han, Chen Gao, Buyou Liang, Jingxuan Cui, Qiyu Xu, Alexander Schulz, Johannes Liesche
Summary: Sucrose is the central unit of carbon and energy in plants, and active intercellular transport is mediated by sucrose transporters (SUTs). In this study, SUTs were cloned from two gymnosperms and their transport activities were confirmed. Results suggest a role for CdSUT1G and CdSUT2 in supporting phloem transport under varying environmental conditions.
Article
Plant Sciences
Anja T. Fuglsang, Michael Palmgren
Summary: Plant plasma membrane H+-ATPases and Ca2+-ATPases are crucial for maintaining low cytoplasmic concentrations of H+ and Ca2+ respectively, essential for plant growth and development. They are finely regulated in response to external stimuli and physiological adaptations.
