A re-sequencing based assessment of genomic heterogeneity and fast neutron-induced deletions in a common be an cultivar

A small fast neutron (FN) mutant population has been established from Phaseolus vulgaris cv. Red Hawk. Weleve ragedthe available P. vulgaris genomes equenceand hight hrough put next generation DNA sequencing to examine the genomic structure of five P. vulgaris cv. Red Hawk FN mutants with striking visual phenotypes. Analysis of these genomes identified three classes of structural variation( SV); between cultivar variation, natural variation within the FN mutant population, and FN induced mutagenesis. Our analyses focused on the latter two classes. We identified 23 large deletions(> 40bp) common to multiple individuals, illustrating residual heterogeneity and regions of SV within the common be ancv. Red Hawk. An additional 18 large deletions were identified in individual mutant plants. These deletions, ranging in size from 40 bpto 43,000 bp, are potentially the result of FN mutagenesis. Six of the 18 deletions lienear or withing enecding regions, identifying potential candidate genes causing the mutant phenotype.