期刊
FRONTIERS IN PLANT SCIENCE
卷 4, 期 -, 页码 -出版社
FRONTIERS RESEARCH FOUNDATION
DOI: 10.3389/fpls.2013.00153
关键词
mitochondria; OXPHOS system; respiratory chain; NADH dehydrogenase; blue-native; BN/SDS/SDS-PAGE; Arabidopsis thaliana
资金
- Wege in die Forschung II program offered by Leibniz University Hannover
- Deutsche Forschungsgemeinschaft (DFG)
- Leibniz Universitat Hannover
Complex I has a unique structure in plants and includes extra subunits. Here, we present a novel study to define its protein constituents. Mitochondria were isolated from Arabidopsis thaliana cell cultures, leaves, and roots. Subunits of complex I were resolved by 3D blue-native (BN)/SDS/SDS-PAGE and identified by mass spectrometry. Overall, 55 distinct proteins were found, seven of which occur in pairs of isoforms. We present evidence that Arabidopsis complex I consists of 49 distinct types of subunits, 40 of which represent homologs of bovine complex I. The nine other subunits represent special proteins absent in the animal linage of eukaryotes, most prominently a group of subunits related to bacterial gamma-type carbonic anhydrases. A GelMap http://www.gelmap.de/arabidopsis-3d-complex-i/ is presented for promoting future complex I research in Arabidopsis thaliana.
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