期刊
FRONTIERS IN PLANT SCIENCE
卷 4, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2013.00339
关键词
molecular cloning; Agrobacterium binary vectors; synthetic biology; combinatorial libraries; Golden Gate Cloning; plant transformation
资金
- National Science Foundation [1157895]
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1157895] Funding Source: National Science Foundation
Tools that allow for rapid, accurate and inexpensive assembly of multi-component combinatorial libraries of DNA for transformation into plants will accelerate the progress of synthetic biology research. Recent innovations in molecular cloning methods has vastly expanded the repertoire with which plant biologists can engineer a transgene. Here we describe a new set of binary vectors for use in Agrobacterium-mediated plant transformation that utilizes the Golden-Gate Cloning approach. Our optimized protocol facilitates the rapid and inexpensive generation of multi-component transgenes for later introduction into plants.
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