Article
Plant Sciences
Hannah J. Lusk, Nicholas Neumann, Madeline Colter, Mary R. Roth, Pamela Tamura, Libin Yao, Sunitha Shiva, Jyoti Shah, Kathrin Schrick, Timothy Durrett, Ruth Welti
Summary: A mass spectrometry-based screening identified a mutant Salk_109175C with altered leaf chloroplast glycerolipid composition. Further analysis revealed that the altered lipid composition phenotype was caused by a splice-site mutation in the At4g30950 locus, which resulted in the functional loss of the plastidial omega-6 desaturase FAD6.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Plant Sciences
Xiaxiang Zhang, Yanli Gao, Lili Zhuang, Bingru Huang
Summary: PA priming can regulate plant tolerance to heat stress and specific lipid species are associated with enhanced heat tolerance. These findings are important for improving heat tolerance in plants and identifying lipid biomarkers.
PLANT GROWTH REGULATION
(2023)
Article
Plant Sciences
Sang-Chul Kim, Shuaibing Yao, Qun Zhang, Xuemin Wang
Summary: This study reveals the cellular and molecular mechanism of heat-induced nuclear translocation of cytosolic GAPC in Arabidopsis thaliana. PLD delta and PA play critical roles in this process, mediated by lipid-protein interaction. These findings suggest a cellular conduit linking stress perturbations at cell membranes to nuclear functions in plants coping with heat stress.
Article
Plant Sciences
Ze Wang, Min Chen, Huan Yang, Zhengdao Hu, Youfeng Yu, Hao Xu, Shunping Yan, Keke Yi, Jing Li
Summary: Haploid production by outcrossing with inducers is a key technology in breeding. Manipulating centromere-specific histone H3 (CENH3/CENPA) has led to the development of a promising haploid inducer. Temperature can improve pollen vigor and haploid induction efficiency, enabling the efficient induction of maternal and paternal haploids.
Article
Biochemical Research Methods
Giorgis Isaac, Ian D. Wilson, Robert S. Plumb
Summary: This study investigated the application of hybrid surface technology (HST) on the metal surfaces of an ACQUITYTM UPLCTM system and column to mitigate the issues of chelation, poor peak shape, and analyte loss caused by acidic phospholipids. The results showed significant improvements in sensitivity, recovery, and peak shape when using HST for the analysis of various phospholipids. The HST system and column particularly improved chromatographic performance at low analyte concentrations. The application of HST also led to reduced peak tailing, narrower peak widths, and increased signal intensities. Similar improvements in data quality were observed when analyzing chicken egg phosphatidic acid (PA) and brain porcine phosphatidylserine (PS) extracts.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Review
Plant Sciences
Oludoyin Adeseun Adigun, Muhammad Nadeem, Thu Huong Pham, Linda Elizabeth Jewell, Mumtaz Cheema, Raymond Thomas
Summary: Plant pathogens are a significant threat to food security and the agricultural economy, with understanding and utilizing plant immune systems being crucial for controlling pathogens and promoting sustainable agriculture. Lipids play a key role in plant defense against pathogens, yet there is limited information available on lipid signaling targets in the scientific literature.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Biochemistry & Molecular Biology
Hieu Sy Vu, Sunitha Shiva, Thilani Samarakoon, Maoyin Li, Sujon Sarowar, Mary R. Roth, Pamela Tamura, Samuel Honey, Kaleb Lowe, Hollie Porras, Neema Prakash, Charles A. Roach, Morgan Stuke, Xuemin Wang, Jyoti Shah, Gary Gadbury, Haiyan Wang, Ruth Welti
Summary: Although the effect of certain lipids on plant freezing tolerance is understood, the impact of many other plant lipids is still unknown. Pre-acclimating plants to non-freezing cold temperature before exposure to freezing temperatures can enhance the outcome of plants. A study on Arabidopsis thaliana plants under different treatments revealed correlations between lipid levels immediately post-freezing and ion leakage, allowing for hypotheses to be generated about the functions of specific lipids. Furthermore, analysis of lipid levels in plants with gene mutations identified differences in lipid composition in the opr3-2 mutant compared to wild-type plants under the same treatments.
Article
Plant Sciences
H. Jochen Schenk, Joseph M. Michaud, Kerri Mocko, Susana Espino, Tatiana Melendres, Mary R. Roth, Ruth Welti, Lucian Kaack, Steven Jansen
Summary: This study analyzed the lipid composition of xylem sap from different plant species and found that phospholipids, galactolipids, and sulfolipids were present in the sap, originating mainly from living vessel cells. Seasonal changes in lipid composition were observed in one species and lipids were found to coat vessel surfaces of certain plant species. The findings suggest that apoplastic xylem lipids are a common feature in angiosperms and may affect water transport dynamics in xylem.
Article
Plant Sciences
Yingjie Bian, Qiuguo Li, Xiaxiang Zhang, Tian Hao, Nanqing Liu, Zhimin Yang, Jingjin Yu
Summary: Heat stress is a detrimental abiotic stress that impedes plant growth and development. Previous studies found that plant roots are more sensitive to heat stress compared to leaves, but the underlying mechanisms are unclear. This study aims to determine whether the differential responses of leaves and roots to heat stress are associated with membrane lipid reprogramming.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Irma Magaly Rivas Serna, Michal Sitina, Gorazd B. Stokin, Jose R. Medina-Inojosa, Francisco Lopez-Jimenez, Juan P. Gonzalez-Rivas, Manlio Vinciguerra
Summary: The study found that good cardiovascular health metrics are associated with a specific pattern of phospho- and sphingolipids. Circulatory lipid profiling may be a potential biomarker to refine cardiovascular health status in primary prevention strategies.
Article
Biochemical Research Methods
Adriana Zardini Buzatto, Abeer Malkawi, Essa M. Sabi, Ahmed H. Mujamammi, Liang Li, Anas M. Abdel Rahman
Summary: Dexamethasone, a synthetic glucocorticoid used to treat abnormal immune responses and inflammation, can have severe adverse effects with prolonged high-dose usage. Comprehensive lipidomic analyses on rat tissues showed significant alterations in phosphatidic acids, glycerophospholipids, plasmalogens, and fatty acids following long-term dexamethasone treatment.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Multidisciplinary Sciences
Shao-Wei Qin, Liang-Hong Bao, Zhi-Gui He, Cai-Lin Li, Hong-gui La, Li-Feng Zhao
Summary: The SPL family plays a significant role in the morphogenesis of heteromorphic leaves in Populus euphratica. They are involved in regulating growth, stress response, photoperiod, and photosynthesis. Non-coding RNAs can promote the expression of SPL genes by co-decoying miR156 in leaf morphogenesis.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Lorena Aranda-Cano, Raquel Valderrama, Mounira Chaki, Juan C. Begara-Morales, Manuel Melguizo, Juan B. Barroso
Summary: The non-enzymatic interaction of polyunsaturated fatty acids with nitric oxide (NO) and derived species results in the formation of nitrated fatty acids (NO2-FAs). These signaling molecules play important roles in plant development and defense responses against abiotic stress conditions. The study identifies previously unknown storage biomolecules of NO2-FAs in Arabidopsis thaliana and determines their distribution during plant development. The esterification of NO2-FAs in phospholipids and proteins highlights their involvement in biomembrane dynamics and signaling processes, respectively.
Article
Biochemistry & Molecular Biology
Daiki Hayashi, Varnavas D. Mouchlis, Edward A. Dennis
Summary: Glycerophospholipids are major components of cell membranes with diverse fatty acyl chain compositions and polar head groups. Phospholipase A(2) enzymes play a critical role in metabolism by hydrolyzing glycerophospholipids, and different human PLA(2) enzymes show distinct preferences for sn-1 acyl chain linkages.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2022)
Article
Multidisciplinary Sciences
Felipe K. Ricachenevsky, Tracy Punshon, David E. Salt, Janette P. Fett, Mary Lou Guerinot
Summary: Zinc is a key micronutrient for plants and animals, and understanding its homeostasis can have positive impacts on agriculture and human health. This study found that the accumulation of zinc in the base of trichomes on leaves is influenced by the overall zinc concentration in the plant, offering a basis for further research on the molecular mechanisms involved in zinc spatial distribution in plants.
SCIENTIFIC REPORTS
(2021)
Article
Plant Sciences
H. Jochen Schenk, Joseph M. Michaud, Kerri Mocko, Susana Espino, Tatiana Melendres, Mary R. Roth, Ruth Welti, Lucian Kaack, Steven Jansen
Summary: This study analyzed the lipid composition of xylem sap from different plant species and found that phospholipids, galactolipids, and sulfolipids were present in the sap, originating mainly from living vessel cells. Seasonal changes in lipid composition were observed in one species and lipids were found to coat vessel surfaces of certain plant species. The findings suggest that apoplastic xylem lipids are a common feature in angiosperms and may affect water transport dynamics in xylem.
Article
Biotechnology & Applied Microbiology
Guangqin Cai, Geliang Wang, Sang-Chul Kim, Jianwu Li, Yongming Zhou, Xuemin Wang
Summary: The study found that increasing the expression levels of FAX1 and ABCA9 could potentially improve oil production and lipid metabolism in Camelina sativa, with their overexpression affecting seed size and lipid synthesis genes.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Review
Biochemistry & Molecular Biology
Yu Song, Zolian S. Zoong Lwe, Pallikonda Arachchige Dona Bashanee Vinusha Wickramasinghe, Ruth Welti
Summary: Head group-acylated chloroplast lipids were discovered in the 1960s, but the interest in this field was renewed about 15 years ago with the discovery of Arabidopsides E and G, leading to the application of mass spectrometry to identify additional oxidized and non-oxidized chloroplast lipids. Despite many advances, there are still critical questions remaining about the biological functions of AGAP1 and its head group-acylated products.
Editorial Material
Biochemistry & Molecular Biology
Joseph M. Jez
Summary: Understanding the pathways of chlorophyll breakdown and the fate of phytol in plants reveals insights into the color changes observed in global plant life during the fall season. Recent research has identified phytenal as an intermediate in plant phytol catabolism, opening up opportunities for further investigation into this complex metabolic system with implications for plant health and agriculture.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Hieu Sy Vu, Sunitha Shiva, Thilani Samarakoon, Maoyin Li, Sujon Sarowar, Mary R. Roth, Pamela Tamura, Samuel Honey, Kaleb Lowe, Hollie Porras, Neema Prakash, Charles A. Roach, Morgan Stuke, Xuemin Wang, Jyoti Shah, Gary Gadbury, Haiyan Wang, Ruth Welti
Summary: Although the effect of certain lipids on plant freezing tolerance is understood, the impact of many other plant lipids is still unknown. Pre-acclimating plants to non-freezing cold temperature before exposure to freezing temperatures can enhance the outcome of plants. A study on Arabidopsis thaliana plants under different treatments revealed correlations between lipid levels immediately post-freezing and ion leakage, allowing for hypotheses to be generated about the functions of specific lipids. Furthermore, analysis of lipid levels in plants with gene mutations identified differences in lipid composition in the opr3-2 mutant compared to wild-type plants under the same treatments.
Article
Plant Sciences
Hannah J. Lusk, Nicholas Neumann, Madeline Colter, Mary R. Roth, Pamela Tamura, Libin Yao, Sunitha Shiva, Jyoti Shah, Kathrin Schrick, Timothy Durrett, Ruth Welti
Summary: A mass spectrometry-based screening identified a mutant Salk_109175C with altered leaf chloroplast glycerolipid composition. Further analysis revealed that the altered lipid composition phenotype was caused by a splice-site mutation in the At4g30950 locus, which resulted in the functional loss of the plastidial omega-6 desaturase FAD6.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Nutrition & Dietetics
Susumu Ishiguro, Mary Roth, Ruth Welti, Mayme Loyd, Ravindra Thakkar, Morgan Phillips, Nicole Robben, Deepa Upreti, Ayaka Nakashima, Kengo Suzuki, Jeffrey Comer, Masaaki Tamura
Summary: This study found that the cell walls of Chlorella sorokiniana contain a lipopolysaccharide-like material, which can stimulate the growth of bone marrow cells and splenocytes, and increase the expression of specific cell subpopulations and cytokines.
Article
Biochemistry & Molecular Biology
Prasad Parchuri, Anjali Pappanoor, Abdulrahman Naeem, Timothy P. Durrett, Ruth Welti, R. V. Sreedhar
Summary: Buglossoides arvensis is a burgeoning oilseed crop with a unique combination of omega-3 and omega-6 poly-unsaturated fatty acids. During seed development, DGATs play a crucial role in enriching TAGs with PUFA compared to PDATs.
Article
Multidisciplinary Sciences
Ruyi Fan, Fen Zhao, Zhou Gong, Yanke Chen, Bao Yang, Chen Zhou, Jie Zhang, Zhangmeng Du, Xuemin Wang, Ping Yin, Liang Guo, Zhu Liu
Summary: This study reports the crystal structure of Arabidopsis NPC4 and reveals that the C-terminal domain (CTD) plays a critical role in NPC4 activity. Through structure-guided biochemical studies, the authors elucidate the molecular mechanism of phospholipid hydrolysis by NPC4 and provide new insights into the phospholipase family members.
NATURE COMMUNICATIONS
(2023)
Correction
Multidisciplinary Sciences
Ruyi Fan, Fen Zhao, Zhou Gong, Yanke Chen, Bao Yang, Chen Zhou, Jie Zhang, Zhangmeng Du, Xuemin Wang, Ping Yin, Liang Guo, Zhu Liu
NATURE COMMUNICATIONS
(2023)
Article
Food Science & Technology
Colin K. Y. Chun, Mary Roth, Ruth Welti, Mark P. Richards, Wei-Wen Hsu, Travis O'Quinn, Michael D. Chao
Summary: This study aimed to investigate the impact of phospholipase A2 (PLA2) and its antibody (aPLA2) on phospholipid hydrolysis, lipid oxidation, and antioxidant capacity in beef. The results showed that PLA2 decreased key phospholipid classes and increased the release of polyunsaturated fatty acids (FFAs). Despite exhibiting strong antioxidant capacity, PLA2 treatment led to increased lipid oxidation. On the other hand, aPLA2 treatment showed potential in reducing lipid oxidation.
Article
Biochemistry & Molecular Biology
Jianwu Li, Yuan Su, Charles A. Shapiro, Daniel P. Schachtman, Xuemin Wang
Summary: A study found that phosphorus deficiency increases seed oil content and alters fatty acid composition of Camelina seeds, but greatly reduces seed production. This suggests that achieving a high yield and quality of Camelina seed oil is closely linked to the phosphorus status of the soil.
Article
Cell Biology
Sang-Chul Kim, Kristen N. Edgeworth, Dmitri A. Nusinow, Xuemin Wang
Summary: This study reveals that the central clock regulators LHY and CCA1 play a role in the initial step of fatty acid synthesis in Arabidopsis. LHY enhances fatty acid synthesis, and overexpression of LHY leads to an increase in triacylglycerol accumulation in seeds.
Article
Biochemistry & Molecular Biology
Bao Yang, Maoyin Li, Anne Phillips, Long Li, Usman Ali, Qing Li, Shaoping Lu, Yueyun Hong, Xuemin Wang, Liang Guo
Summary: Phosphate is essential for plant growth, and NPC4 enzyme plays a positive role in root growth by hydrolyzing and remodeling sphingolipids during phosphate deficiency in Arabidopsis.