Article
Biochemistry & Molecular Biology
Alicja W. Nowakowska, Jakub W. Wojciechowski, Natalia Szulc, Malgorzata Kotulska
Summary: Repetitivity and modularity are important for the functional properties of amyloids. Functional amyloids are proteins that have different characteristics from pathological amyloids. Repetitive sequences help regulate the aggregation propensity of functional amyloids and strengthen their desirable structural properties. Our study on bacterial functional amyloids suggests that similar mechanisms may be employed in other functional amyloids to control their fibril formation and stability.
JOURNAL OF STRUCTURAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Thorbjorn Sonderby, Nikolaos N. Louros, Ladan Khodaparast, Laleh Khodaparast, Daniel J. Madsen, William P. Olsen, Nele Moonen, Madhu Nagaraj, Vita Sereikaite, Kristian Stromgaard, Frederic Rousseau, Joost Schymkowitz, Daniel E. Otzen
Summary: Functional bacterial amyloid provides structural stability in biofilm, which can be targeted for anti-biofilm therapeutics. Aggregation-modulating peptides can be used to disrupt the stability and morphology of amyloid proteins, such as CsgA and FapC, resulting in reduced biofilm formation in E. coli and P. aeruginosa. This study demonstrates the potential of selective amyloid targeting in combating bacterial biofilm.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Sujeet S. Bhoite, Divya Kolli, Mark A. Gomulinski, Matthew R. Chapman
Summary: Bacterial biofilm formation can be influenced by the charge state of gatekeeper residues in the CsgA protein, which is a major component of curli amyloid fibers produced by enteric bacteria. A mechanism of gatekeeping is proposed where certain arginine and lysine residues interact with negatively charged aspartic acid residues, tempering CsgA fiber formation. This finding expands our understanding of the factors that regulate biofilm formation.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Microbiology
George Tetz, Victor Tetz
Summary: The study found that bacterial extracellular DNA can trigger Aβ protein aggregation, with the acceleration depending on the DNA concentration and bacterial strain. It suggests that bacterial DNA may play an important role in the pathogenesis of Alzheimer's disease.
Article
Biochemistry & Molecular Biology
Macarena Siri, Melisa Herrera, Alejandro J. Moyano, M. Soledad Celej
Summary: Bacterial biofilms represent an alternative lifestyle for bacteria, and understanding the role of amyloid fibrils in these communities can provide insights into controlling biofilm formation. The study focused on FapC as a novel target to control Pseudomonas biofilms, and highlighted the impact of alginate on the morphology of FapC fibrils. This research emphasizes the importance of considering the complexity of the extracellular matrix in studying microbial functional amyloids.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Review
Biochemistry & Molecular Biology
Thorbjorn Vincent Sonderby, Zahra Najarzadeh, Daniel Erik Otzen
Summary: Functional amyloid is produced by various organisms, particularly well-understood in bacteria. Its formation is influenced by repeat sequences and helper proteins. Smart peptides and small molecules can be used to reduce biofilm formation. Additionally, functional amyloid can form two-dimensional films on structured surfaces, suggesting potential applications in biosensors and nanobiomaterials.
Article
Microbiology
Shakilur Rahman, Amit Kumar Das
Summary: Staphylococcus aureus is a highly infectious pathogen that forms biofilms, reducing susceptibility to antibiotics and immune defenses. A study has identified a novel function of staphylococcal superantigen-like protein 10 (SSL10) in enhancing biofilm formation, through amyloid aggregation.
Article
Microbiology
Mnar Ghrayeb, Shahar Hayet, Neta Lester-Zer, Yael Levi-Kalisman, Liraz Chai
Summary: Functional amyloid proteins are essential components in microbial biofilms, providing mechanical stability, mediating cell adhesion, and regulating biofilm development. The morphology of amyloid fibers, such as TasA, can vary based on environmental conditions and may serve different functional roles in biofilms, from mechanical support to acting as antibiotic agents.
Article
Chemistry, Multidisciplinary
Yijing Tang, Dong Zhang, Xiong Gong, Jie Zheng
Summary: This study demonstrates a rational design strategy of an amyloid-aggregation-induced emission (AIE)-active molecule, G7-TBA, which serves as a multiple-target, dual-function amyloid probe and modulator for detecting, monitoring, and altering amyloid aggregation of different amyloid proteins.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Review
Biochemistry & Molecular Biology
Anna Sulatskaya, Anastasiia O. Kosolapova, Alexander G. Bobylev, Mikhail Belousov, Kirill S. Antonets, Maksim Sulatsky, Irina M. Kuznetsova, Konstantin K. Turoverov, Olesya Stepanenko, Anton A. Nizhnikov
Summary: Both amyloids and beta-barrel proteins have beta-sheet-rich structures, with the latter being able to form functional amyloids in vivo. These beta-barrel amyloid proteins can interact with each other and form toxic oligomers, potentially contributing to the development of amyloidoses. Rapidly growing discoveries suggest that the number and diversity of functions of amyloid-forming beta-barrel proteins are significantly greater than currently understood.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Michael C. Wilson, Qin Lu, Kaitlin R. Nachtrieb, Jackson S. Fuller, Chloe M. Skogg, Elizabeth A. Yates, Matthew D. Thum, Christopher R. So
Summary: This study demonstrates the chemical aggregation of common hydrophilic proteins into an aqueous adhesive similar to natural adhesives. By using multiple chemical denaturants, rapid gelation and further curing in artificial seawater can be achieved. The adhesive strengths of this waterborne adhesive are comparable to bioinspired adhesives that use organic solvents and hydrophobic components. This approach enables the deployment of a bioinspired adhesive at practical scales in marine environments, with sustainable production and low-cost materials.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Review
Biochemical Research Methods
R. Prabakaran, Puneet Rawat, Sandeep Kumar, M. Michael Gromiha
Summary: This review provides a rigorous performance analysis of nine prediction tools for protein and peptide aggregation, revealing the robustness of current prediction tools and their potential for improvement. Insights gained from this work offer critical guidance to the scientific community on the advantages and limitations of different aggregation prediction methods.
BRIEFINGS IN BIOINFORMATICS
(2021)
Article
Biochemistry & Molecular Biology
Alexander G. Bobylev, Roman S. Fadeev, Liya G. Bobyleva, Margarita I. Kobyakova, Yuri M. Shlyapnikov, Daniil V. Popov, Ivan M. Vikhlyantsev
Summary: The study found that amyloid aggregates of smooth-muscle titin can impair cell adhesion and lead to cell death. The surface roughness may be a key factor contributing to the highly antiadhesive properties. The negative impact of amyloid aggregates on cell adhesion is likely intrinsic to other amyloid proteins with similar structure and properties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Kamile Mikalauskaite, Mantas Ziaunys, Vytautas Smirnovas
Summary: This study examines the impact of the initial folding state of a protein on amyloid fibril formation, revealing a correlation between protein state and the kinetics and structural properties of the resulting fibrils.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Jiangtao Zhou, Ting Li, Mohammad Peydayesh, Mattia Usuelli, Viviane Lutz-Bueno, Jie Teng, Li Wang, Raffaele Mezzenga
Summary: Amyloid functional materials derived from oat globulin show diverse functionalities with rich polymorphism, reversible and irreversible fibrillization processes. These materials can be applied in water purification, sensors, and electrodes, with demonstrated sustainability against other protein sources for environmentally-efficient advanced materials and technologies.
Article
Immunology
Adnan K. Syed, Tamra J. Reed, Kaitlyn L. Clark, Blaise R. Boles, J. Michelle Kahlenberg
INFECTION AND IMMUNITY
(2015)
Article
Multidisciplinary Sciences
Alicia S. DeFrancesco, Nadezda Masloboeva, Adnan K. Syed, Aaron DeLoughery, Niels Bradshaw, Gene-Wei Li, Michael S. Gilmore, Suzanne Walker, Richard Losick
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Biotechnology & Applied Microbiology
William H. DePas, Adnan K. Syed, Margarita Sifuentes, John S. Lee, David Warshaw, Vinay Saggar, Gyoergyi Csankovszki, Blaise R. Boles, Matthew R. Chapman
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2014)
Article
Immunology
Kelly Schwartz, Matthew D. Sekedat, Adnan K. Syed, Brendan O'Hara, David E. Payne, Abigail Lamb, Blaise R. Boles
INFECTION AND IMMUNITY
(2014)
Article
Biochemistry & Molecular Biology
Benjamin Peret, Kamal Swarup, Alison Ferguson, Malvika Seth, Yaodong Yang, Stijn Dhondt, Nicholas James, Ilda Casimiro, Paula Perry, Adnan Syed, Haibing Yang, Jesica Reemmer, Edward Venison, Caroline Howells, Miguel A. Perez-Amador, Jeonga Yun, Jose Alonso, Gerrit T. S. Beemster, Laurent Laplaze, Angus Murphy, Malcolm J. Bennett, Erik Nielsen, Ranjan Swarup
Article
Microbiology
Adnan K. Syed, Sudeshna Ghosh, Nancy G. Love, Blaise R. Boles
Article
Microbiology
Kelly Schwartz, Adnan K. Syed, Rachel E. Stephenson, Alexander H. Rickard, Blaise R. Boles