Article
Immunology
Allyson E. Shea, Jolie A. Stocki, Stephanie D. Himpsl, Sara N. Smith, Harry L. T. Mobley
Summary: Uropathogenic Escherichia coli (UPEC) causes urinary tract infections (UTI) in nearly half of women worldwide, and the role of the ila locus in UPEC uropathogenesis remains unclear. This study found that the ila locus is related to bladder cell adherence and invasion, and its loss may affect the fitness of UPEC during UTI.
INFECTION AND IMMUNITY
(2022)
Article
Microbiology
Caroline Mullineaux-Sanders, Danielle Carson, Eve G. D. Hopkins, Izabela Glegola-Madejska, Alejandra Escobar-Zepeda, Hilary P. Browne, Trevor D. Lawley, Gad Frankel
Summary: The gut microbiota plays a crucial role in combating enteric pathogens, and the phenomenon of antibiotic-induced bacterial commensalization suggests that commensal species can provide colonization resistance. This highlights the significance of studying and utilizing commensal bacteria in researching and preventing intestinal infections.
Review
Biochemistry & Molecular Biology
Julia Sanchez-Garrido, David Ruano-Gallego, Jyoti S. Choudhary, Gad Frankel
Summary: Recent studies have shown that T3SS effectors interact with each other in the host, revealing their interdependency and context-dependent essentiality, which is of great significance for understanding the pathogenesis of the pathogens.
TRENDS IN MICROBIOLOGY
(2022)
Article
Oncology
Yue Liu, Kai Fu, Eric M. Wier, Yifan Lei, Andrea Hodgson, Dongqing Xu, Xue Xia, Dandan Zheng, Hua Ding, Cynthia L. Sears, Jian Yang, Fengyi Wan
Summary: This study identified a novel genotoxin called UshA in attaching/effacing pathogens, which triggers DNA damage and initiates tumorigenic transformation during bacterial infections. Furthermore, it was found that UshA plays a critical role in accelerating colon tumorigenesis in mice. These findings highlight the importance of UshA in the development of colon cancer caused by bacterial infections.
Article
Microbiology
Alyson R. Warr, Carole J. Kuehl, Matthew K. Waldor
Summary: The study demonstrates that the Shiga toxin (Stx) released by Enterohemorrhagic Escherichia coli (EHEC) markedly remodels how epithelial cells respond to infection, particularly in terms of the intestinal innate immune response.
Article
Immunology
Lydia A. Sweet, Sharon K. Kuss-Duerkop, A. Marijke Keestra-Gounder
Summary: ER stress and the IRE1 alpha pathway play crucial roles in inducing inflammation during bacterial infection.
INFECTION AND IMMUNITY
(2022)
Article
Microbiology
Alejandro Huerta-Saquero, Itziar Chapartegui-Gonzalez, Sarah Bowser, Nittaya Khakhum, Jacob L. Stockton, Alfredo G. Torres
Summary: This study demonstrates the potential of using P22 viral-like particles as nanocontainers for delivering immunogenic proteins of enterohemorrhagic Escherichia coli (EHEC) and creating effective vaccines against EHEC infections. The EscC-P22 particles were able to induce specific antibodies in mice, enhance phagocytic activity against EHEC, and reduce bacterial adherence to epithelial cells, indicating their functionality. These findings suggest that EscC-P22-based particles could be a promising nanovaccine candidate for immunization against EHEC O157:H7 infections.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Lang Peng, Rexford Mawunyo Dumevi, Marco Chitto, Nadja Haarmann, Petya Berger, Gerald Koudelka, Herbert Schmidt, Alexander Mellmann, Ulrich Dobrindt, Michael Berger
Summary: This study proposes a new method for genetic manipulation of enterohemorrhagic Escherichia coli (EHEC) that is more efficient than traditional methods. The method increases recombineering efficiency by 3-4 times in EHEC O157:H7 EDL933 Delta stx1/2, but shows similar efficiency in Escherichia coli K-12 MG1655. Additionally, the study suggests that the inhibition of the RecFOR pathway indirectly blocks phage induction and improves recombineering efficiency.
Article
Gastroenterology & Hepatology
Naheed Choudhry, Flora Scott, Meghan Edgar, Gareth J. Sanger, Paul Kelly
Summary: Environmental enteropathy is associated with chronic low-grade intestinal inflammation and disrupted epithelial barrier integrity, leading to bacterial translocation. Contra-pathogenicity therapies can mitigate enteropathogen-mediated damage to the intestinal epithelial barrier.
DIGESTIVE DISEASES AND SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
M. Beukema, K. Ishisono, J. de Waard, M. M. Faas, P. de Vos, K. Kitaguchi
Summary: Pectin exhibits antimicrobial effects, protecting the epithelial barrier from damage caused by C. rodentium, and also reduces its adhesive properties to epithelial cells.
Review
Gastroenterology & Hepatology
Lingyan Jiang, Wen Yang, Xinlei Jiang, Ting Yao, Lu Wang, Bin Yang
Summary: EHEC O157 carries 177 unique genomic islands, termed O islands, which play a crucial role in its pathogenicity by providing virulence factors, effectors, and regulatory proteins.
Article
Gastroenterology & Hepatology
Jennifer Lising Roxas, Shylaja Ramamurthy, Katie Cocchi, Ilga Rutins, Anusha Harishankar, Al Agellon, John Scott Wilbur, Gresa Sylejmani, Gayatri Vedantam, V. K. Viswanathan
Summary: Bacterial pathogens manipulate host cell structures and functions, contributing to disease symptoms. Enteropathogenic Escherichia coli (EPEC) secretes proteins that alter host cell behavior. Two secreted proteins, EspZ and EspH, have contrasting effects on host mitochondria. EspZ interacts with FIS1 and protects the host mitochondrial network, enhancing host cell viability. In contrast, EspH increases FIS1 and leads to significant mitochondrial fragmentation and host cell death, facilitating pathogen dispersal.
Review
Food Science & Technology
Xiaoyan Zhao, Yeting Sun, Yue Ma, Yujia Xu, Hongyang Guan, Dan Wang
Summary: Enterohemorrhagic Escherichia coli (EHEC) is a major bacterial pathogen that can survive in food such as meat and vegetables. Understanding the impact of vegetable characteristics on the adhesion and proliferation processes of EHEC is important for developing control measures. This review discusses the amount, methods, and pathways of adhesion, as well as the proliferation and biofilm formation processes of EHEC during vegetable contamination. Factors such as vegetable types, cultivars, tissue characteristics, and damage levels can affect EHEC adhesion on vegetables.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Biochemistry & Molecular Biology
Jorge Soria-Bustos, Zeus Saldana-Ahuactzi, Partha Samadder, Jorge A. Yanez-Santos, Ygnacio Martinez Laguna, Maria L. Cedillo-Ramirez, Jorge A. Giron
Summary: This study investigates the relationship between flagella expression and the type III secretion system (T3SS) in enteropathogenic Escherichia coli (EPEC). The results suggest that the assembly of an intact T3SS is required for flagella production, and certain regulator genes play a role in activating or inhibiting flagella expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Veterinary Sciences
Delphine Bibbal, Philippe Ruiz, Panagiotis Sapountzis, Christine Mazuy-Cruchaudet, Estelle Loukiadis, Frederic Auvray, Evelyne Forano, Hubert Brugere
Summary: This study aimed to identify farms with persistent circulation of EHEC O157:H7 in cattle. The results showed that persistent circulation may be due to the presence of a few predominant strains or repeated exposure of cattle to various strains. In addition, differences were found in the fecal microbial communities of shedders and non-shedders, with different bacterial indicators identified.
FRONTIERS IN VETERINARY SCIENCE
(2022)